Experiment: 5-Hydroxymethylfurfural 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2382 and Pf6N2E2_2383 are separated by 108 nucleotides Pf6N2E2_2383 and Pf6N2E2_2384 are separated by 295 nucleotides Pf6N2E2_2384 and Pf6N2E2_2385 are separated by 2 nucleotides Pf6N2E2_2385 and Pf6N2E2_2386 are separated by 63 nucleotides
Pf6N2E2_2382 - Major porin and structural outer membrane porin OprF, at 2,821,381 to 2,822,415
_2382
Pf6N2E2_2383 - DNA-directed RNA polymerase specialized sigma subunit, sigma24-like, at 2,822,524 to 2,822,910
_2383
Pf6N2E2_2384 - CmpX, at 2,823,206 to 2,824,030
_2384
Pf6N2E2_2385 - CrfX protein, at 2,824,033 to 2,824,278
_2385
Pf6N2E2_2386 - Magnesium and cobalt transport protein CorA, at 2,824,342 to 2,825,337
_2386
Position (kb)
2823
2824
2825 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2822.498 kb on - strand at 2822.504 kb on - strand at 2822.504 kb on - strand at 2823.095 kb on - strand at 2823.136 kb on - strand at 2823.185 kb on - strand at 2823.185 kb on - strand at 2823.185 kb on - strand at 2823.185 kb on - strand at 2823.185 kb on - strand at 2823.355 kb on - strand, within Pf6N2E2_2384 at 2823.370 kb on - strand, within Pf6N2E2_2384 at 2823.370 kb on - strand, within Pf6N2E2_2384 at 2823.370 kb on - strand, within Pf6N2E2_2384 at 2823.518 kb on - strand, within Pf6N2E2_2384 at 2823.518 kb on - strand, within Pf6N2E2_2384 at 2823.518 kb on - strand, within Pf6N2E2_2384 at 2823.689 kb on - strand, within Pf6N2E2_2384 at 2823.756 kb on - strand, within Pf6N2E2_2384 at 2823.756 kb on - strand, within Pf6N2E2_2384 at 2823.756 kb on - strand, within Pf6N2E2_2384 at 2823.965 kb on - strand at 2823.965 kb on - strand at 2823.969 kb on - strand at 2824.030 kb on + strand at 2824.031 kb on - strand at 2824.045 kb on - strand at 2824.060 kb on - strand, within Pf6N2E2_2385 at 2824.523 kb on + strand, within Pf6N2E2_2386 at 2824.523 kb on + strand, within Pf6N2E2_2386 at 2824.524 kb on - strand, within Pf6N2E2_2386 at 2824.650 kb on - strand, within Pf6N2E2_2386 at 2824.787 kb on + strand, within Pf6N2E2_2386 at 2824.787 kb on + strand, within Pf6N2E2_2386 at 2824.788 kb on - strand, within Pf6N2E2_2386 at 2824.788 kb on - strand, within Pf6N2E2_2386 at 2824.788 kb on - strand, within Pf6N2E2_2386 at 2824.886 kb on + strand, within Pf6N2E2_2386 at 2824.887 kb on - strand, within Pf6N2E2_2386
Per-strain Table
Position Strand Gene LocusTag Fraction 5-Hydroxymethylfurfural 10 mM remove 2,822,498 - -1.1 2,822,504 - -1.0 2,822,504 - +0.3 2,823,095 - -0.6 2,823,136 - +0.7 2,823,185 - -1.0 2,823,185 - +0.3 2,823,185 - -0.4 2,823,185 - -0.2 2,823,185 - +0.3 2,823,355 - Pf6N2E2_2384 0.18 +0.5 2,823,370 - Pf6N2E2_2384 0.20 -2.2 2,823,370 - Pf6N2E2_2384 0.20 -0.8 2,823,370 - Pf6N2E2_2384 0.20 -1.3 2,823,518 - Pf6N2E2_2384 0.38 -2.2 2,823,518 - Pf6N2E2_2384 0.38 -3.1 2,823,518 - Pf6N2E2_2384 0.38 -1.8 2,823,689 - Pf6N2E2_2384 0.59 -0.4 2,823,756 - Pf6N2E2_2384 0.67 -1.7 2,823,756 - Pf6N2E2_2384 0.67 -0.5 2,823,756 - Pf6N2E2_2384 0.67 -0.3 2,823,965 - -1.7 2,823,965 - +0.0 2,823,969 - -0.1 2,824,030 + +1.4 2,824,031 - -0.7 2,824,045 - -0.5 2,824,060 - Pf6N2E2_2385 0.11 -1.1 2,824,523 + Pf6N2E2_2386 0.18 -1.0 2,824,523 + Pf6N2E2_2386 0.18 +1.0 2,824,524 - Pf6N2E2_2386 0.18 +0.4 2,824,650 - Pf6N2E2_2386 0.31 -0.5 2,824,787 + Pf6N2E2_2386 0.45 -0.6 2,824,787 + Pf6N2E2_2386 0.45 +0.2 2,824,788 - Pf6N2E2_2386 0.45 +0.3 2,824,788 - Pf6N2E2_2386 0.45 +0.6 2,824,788 - Pf6N2E2_2386 0.45 -1.9 2,824,886 + Pf6N2E2_2386 0.55 +0.4 2,824,887 - Pf6N2E2_2386 0.55 -0.6
Or see this region's nucleotide sequence