Experiment: 5-Hydroxymethylfurfural 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2316 and Pf6N2E2_2317 are separated by 11 nucleotides Pf6N2E2_2317 and Pf6N2E2_2318 are separated by 179 nucleotides Pf6N2E2_2318 and Pf6N2E2_2319 are separated by 24 nucleotides Pf6N2E2_2319 and Pf6N2E2_2320 overlap by 8 nucleotides
Pf6N2E2_2316 - Type cbb3 cytochrome oxidase biogenesis protein CcoG, involved in Cu oxidation, at 2,738,597 to 2,740,012
_2316
Pf6N2E2_2317 - Putative analog of CcoH, COG3198, at 2,740,024 to 2,740,563
_2317
Pf6N2E2_2318 - Type cbb3 cytochrome oxidase biogenesis protein CcoI; Copper-translocating P-type ATPase (EC 3.6.3.4), at 2,740,743 to 2,743,193
_2318
Pf6N2E2_2319 - Type cbb3 cytochrome oxidase biogenesis protein CcoS, involved in heme b insertion, at 2,743,218 to 2,743,436
_2319
Pf6N2E2_2320 - Heavy-metal-associated domain (N-terminus) and membrane-bounded cytochrome biogenesis cycZ-like domain, possible membrane copper tolerance protein, at 2,743,429 to 2,744,112
_2320
Position (kb)
2740
2741
2742
2743
2744 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2739.787 kb on + strand, within Pf6N2E2_2316 at 2739.787 kb on + strand, within Pf6N2E2_2316 at 2739.787 kb on + strand, within Pf6N2E2_2316 at 2739.788 kb on - strand, within Pf6N2E2_2316 at 2739.788 kb on - strand, within Pf6N2E2_2316 at 2739.788 kb on - strand, within Pf6N2E2_2316 at 2739.788 kb on - strand, within Pf6N2E2_2316 at 2739.788 kb on - strand, within Pf6N2E2_2316 at 2739.788 kb on - strand, within Pf6N2E2_2316 at 2739.791 kb on + strand, within Pf6N2E2_2316 at 2739.791 kb on + strand, within Pf6N2E2_2316 at 2739.791 kb on + strand, within Pf6N2E2_2316 at 2739.791 kb on + strand, within Pf6N2E2_2316 at 2739.791 kb on + strand, within Pf6N2E2_2316 at 2739.791 kb on + strand, within Pf6N2E2_2316 at 2739.791 kb on + strand, within Pf6N2E2_2316 at 2739.791 kb on + strand, within Pf6N2E2_2316 at 2739.791 kb on + strand, within Pf6N2E2_2316 at 2739.791 kb on + strand, within Pf6N2E2_2316 at 2739.792 kb on - strand, within Pf6N2E2_2316 at 2739.792 kb on - strand, within Pf6N2E2_2316 at 2739.792 kb on - strand, within Pf6N2E2_2316 at 2739.792 kb on - strand, within Pf6N2E2_2316 at 2739.792 kb on - strand, within Pf6N2E2_2316 at 2739.792 kb on - strand, within Pf6N2E2_2316 at 2739.792 kb on - strand, within Pf6N2E2_2316 at 2739.792 kb on - strand, within Pf6N2E2_2316 at 2739.869 kb on + strand, within Pf6N2E2_2316 at 2739.870 kb on - strand, within Pf6N2E2_2316 at 2739.870 kb on - strand, within Pf6N2E2_2316 at 2739.870 kb on - strand, within Pf6N2E2_2316 at 2739.870 kb on - strand, within Pf6N2E2_2316 at 2739.966 kb on + strand at 2739.966 kb on + strand at 2739.967 kb on - strand at 2739.967 kb on - strand at 2740.054 kb on + strand at 2740.054 kb on + strand at 2740.055 kb on - strand at 2740.055 kb on - strand at 2740.055 kb on - strand at 2740.168 kb on + strand, within Pf6N2E2_2317 at 2740.168 kb on + strand, within Pf6N2E2_2317 at 2740.168 kb on + strand, within Pf6N2E2_2317 at 2740.168 kb on + strand, within Pf6N2E2_2317 at 2740.169 kb on - strand, within Pf6N2E2_2317 at 2740.169 kb on - strand, within Pf6N2E2_2317 at 2740.171 kb on + strand, within Pf6N2E2_2317 at 2740.171 kb on + strand, within Pf6N2E2_2317 at 2740.172 kb on - strand, within Pf6N2E2_2317 at 2740.263 kb on + strand, within Pf6N2E2_2317 at 2740.561 kb on + strand at 2740.561 kb on + strand at 2740.561 kb on + strand at 2740.625 kb on + strand at 2740.625 kb on + strand at 2740.625 kb on + strand at 2740.764 kb on + strand at 2740.893 kb on + strand at 2740.896 kb on + strand at 2740.896 kb on + strand at 2740.897 kb on - strand at 2740.897 kb on - strand at 2740.968 kb on + strand at 2740.968 kb on + strand at 2740.968 kb on + strand at 2740.969 kb on - strand at 2740.969 kb on - strand at 2740.969 kb on - strand at 2740.969 kb on - strand at 2740.969 kb on - strand at 2741.086 kb on + strand, within Pf6N2E2_2318 at 2741.086 kb on + strand, within Pf6N2E2_2318 at 2741.087 kb on - strand, within Pf6N2E2_2318 at 2741.087 kb on - strand, within Pf6N2E2_2318 at 2741.146 kb on + strand, within Pf6N2E2_2318 at 2741.146 kb on + strand, within Pf6N2E2_2318 at 2741.147 kb on - strand, within Pf6N2E2_2318 at 2741.147 kb on - strand, within Pf6N2E2_2318 at 2741.147 kb on - strand, within Pf6N2E2_2318 at 2741.147 kb on - strand, within Pf6N2E2_2318 at 2741.424 kb on + strand, within Pf6N2E2_2318 at 2741.424 kb on + strand at 2741.424 kb on + strand, within Pf6N2E2_2318 at 2741.424 kb on + strand, within Pf6N2E2_2318 at 2741.424 kb on + strand, within Pf6N2E2_2318 at 2741.425 kb on - strand, within Pf6N2E2_2318 at 2741.425 kb on - strand, within Pf6N2E2_2318 at 2741.425 kb on - strand, within Pf6N2E2_2318 at 2741.425 kb on - strand, within Pf6N2E2_2318 at 2741.425 kb on - strand, within Pf6N2E2_2318 at 2741.425 kb on - strand, within Pf6N2E2_2318 at 2741.426 kb on + strand, within Pf6N2E2_2318 at 2741.520 kb on + strand, within Pf6N2E2_2318 at 2741.520 kb on + strand, within Pf6N2E2_2318 at 2741.520 kb on + strand, within Pf6N2E2_2318 at 2741.547 kb on - strand, within Pf6N2E2_2318 at 2741.566 kb on - strand, within Pf6N2E2_2318 at 2741.566 kb on - strand, within Pf6N2E2_2318 at 2741.578 kb on + strand, within Pf6N2E2_2318 at 2741.844 kb on + strand, within Pf6N2E2_2318 at 2742.392 kb on + strand, within Pf6N2E2_2318 at 2742.392 kb on + strand, within Pf6N2E2_2318 at 2743.020 kb on + strand at 2743.020 kb on + strand at 2743.020 kb on + strand at 2743.021 kb on - strand at 2743.021 kb on - strand at 2743.021 kb on - strand at 2743.021 kb on - strand at 2743.062 kb on - strand at 2743.334 kb on + strand, within Pf6N2E2_2319 at 2743.582 kb on + strand, within Pf6N2E2_2320 at 2743.582 kb on + strand, within Pf6N2E2_2320 at 2743.606 kb on + strand, within Pf6N2E2_2320 at 2743.607 kb on - strand, within Pf6N2E2_2320 at 2743.607 kb on - strand, within Pf6N2E2_2320 at 2743.717 kb on + strand, within Pf6N2E2_2320 at 2743.718 kb on - strand, within Pf6N2E2_2320
Per-strain Table
Position Strand Gene LocusTag Fraction 5-Hydroxymethylfurfural 10 mM remove 2,739,787 + Pf6N2E2_2316 0.84 +0.0 2,739,787 + Pf6N2E2_2316 0.84 -0.1 2,739,787 + Pf6N2E2_2316 0.84 -1.2 2,739,788 - Pf6N2E2_2316 0.84 -0.3 2,739,788 - Pf6N2E2_2316 0.84 -0.9 2,739,788 - Pf6N2E2_2316 0.84 -1.4 2,739,788 - Pf6N2E2_2316 0.84 -1.0 2,739,788 - Pf6N2E2_2316 0.84 -1.0 2,739,788 - Pf6N2E2_2316 0.84 -2.6 2,739,791 + Pf6N2E2_2316 0.84 -0.5 2,739,791 + Pf6N2E2_2316 0.84 -0.1 2,739,791 + Pf6N2E2_2316 0.84 -0.5 2,739,791 + Pf6N2E2_2316 0.84 -1.7 2,739,791 + Pf6N2E2_2316 0.84 -0.5 2,739,791 + Pf6N2E2_2316 0.84 -1.1 2,739,791 + Pf6N2E2_2316 0.84 -0.9 2,739,791 + Pf6N2E2_2316 0.84 -0.6 2,739,791 + Pf6N2E2_2316 0.84 -1.0 2,739,791 + Pf6N2E2_2316 0.84 -0.3 2,739,792 - Pf6N2E2_2316 0.84 -1.1 2,739,792 - Pf6N2E2_2316 0.84 -1.5 2,739,792 - Pf6N2E2_2316 0.84 -0.7 2,739,792 - Pf6N2E2_2316 0.84 +0.6 2,739,792 - Pf6N2E2_2316 0.84 -1.5 2,739,792 - Pf6N2E2_2316 0.84 -0.3 2,739,792 - Pf6N2E2_2316 0.84 -2.0 2,739,792 - Pf6N2E2_2316 0.84 +0.5 2,739,869 + Pf6N2E2_2316 0.90 +0.1 2,739,870 - Pf6N2E2_2316 0.90 -2.3 2,739,870 - Pf6N2E2_2316 0.90 -0.8 2,739,870 - Pf6N2E2_2316 0.90 -1.2 2,739,870 - Pf6N2E2_2316 0.90 -0.9 2,739,966 + -1.0 2,739,966 + -0.4 2,739,967 - -1.1 2,739,967 - +1.1 2,740,054 + -0.3 2,740,054 + +2.8 2,740,055 - +0.0 2,740,055 - -2.1 2,740,055 - -1.0 2,740,168 + Pf6N2E2_2317 0.27 +0.2 2,740,168 + Pf6N2E2_2317 0.27 -1.5 2,740,168 + Pf6N2E2_2317 0.27 -0.0 2,740,168 + Pf6N2E2_2317 0.27 -2.0 2,740,169 - Pf6N2E2_2317 0.27 -1.9 2,740,169 - Pf6N2E2_2317 0.27 -1.6 2,740,171 + Pf6N2E2_2317 0.27 +0.0 2,740,171 + Pf6N2E2_2317 0.27 -0.8 2,740,172 - Pf6N2E2_2317 0.27 -0.5 2,740,263 + Pf6N2E2_2317 0.44 -1.8 2,740,561 + +0.6 2,740,561 + -1.0 2,740,561 + +0.4 2,740,625 + +0.4 2,740,625 + -0.1 2,740,625 + -0.1 2,740,764 + -1.0 2,740,893 + -3.0 2,740,896 + -2.0 2,740,896 + -1.3 2,740,897 - -2.2 2,740,897 - -2.8 2,740,968 + -2.3 2,740,968 + +0.0 2,740,968 + +0.0 2,740,969 - -2.8 2,740,969 - -1.7 2,740,969 - -0.2 2,740,969 - +0.5 2,740,969 - -2.6 2,741,086 + Pf6N2E2_2318 0.14 -2.3 2,741,086 + Pf6N2E2_2318 0.14 -2.7 2,741,087 - Pf6N2E2_2318 0.14 -1.8 2,741,087 - Pf6N2E2_2318 0.14 -3.2 2,741,146 + Pf6N2E2_2318 0.16 -2.0 2,741,146 + Pf6N2E2_2318 0.16 -2.2 2,741,147 - Pf6N2E2_2318 0.16 -1.7 2,741,147 - Pf6N2E2_2318 0.16 -2.5 2,741,147 - Pf6N2E2_2318 0.16 -1.2 2,741,147 - Pf6N2E2_2318 0.16 -2.5 2,741,424 + Pf6N2E2_2318 0.28 -2.2 2,741,424 + +1.2 2,741,424 + Pf6N2E2_2318 0.28 -3.0 2,741,424 + Pf6N2E2_2318 0.28 -1.9 2,741,424 + Pf6N2E2_2318 0.28 -0.9 2,741,425 - Pf6N2E2_2318 0.28 -0.9 2,741,425 - Pf6N2E2_2318 0.28 -3.4 2,741,425 - Pf6N2E2_2318 0.28 -1.7 2,741,425 - Pf6N2E2_2318 0.28 -1.4 2,741,425 - Pf6N2E2_2318 0.28 -3.5 2,741,425 - Pf6N2E2_2318 0.28 -3.3 2,741,426 + Pf6N2E2_2318 0.28 -0.6 2,741,520 + Pf6N2E2_2318 0.32 -0.4 2,741,520 + Pf6N2E2_2318 0.32 -3.4 2,741,520 + Pf6N2E2_2318 0.32 -1.5 2,741,547 - Pf6N2E2_2318 0.33 -1.9 2,741,566 - Pf6N2E2_2318 0.34 -1.4 2,741,566 - Pf6N2E2_2318 0.34 -0.6 2,741,578 + Pf6N2E2_2318 0.34 -2.6 2,741,844 + Pf6N2E2_2318 0.45 -1.7 2,742,392 + Pf6N2E2_2318 0.67 -3.0 2,742,392 + Pf6N2E2_2318 0.67 -1.5 2,743,020 + -1.2 2,743,020 + -2.1 2,743,020 + -2.5 2,743,021 - -1.2 2,743,021 - +0.8 2,743,021 - -0.4 2,743,021 - -2.4 2,743,062 - -0.4 2,743,334 + Pf6N2E2_2319 0.53 -1.1 2,743,582 + Pf6N2E2_2320 0.22 -2.3 2,743,582 + Pf6N2E2_2320 0.22 +0.2 2,743,606 + Pf6N2E2_2320 0.26 -0.6 2,743,607 - Pf6N2E2_2320 0.26 -1.8 2,743,607 - Pf6N2E2_2320 0.26 -2.7 2,743,717 + Pf6N2E2_2320 0.42 -1.0 2,743,718 - Pf6N2E2_2320 0.42 -3.7
Or see this region's nucleotide sequence