Experiment: 5-Hydroxymethylfurfural 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2310 and Pf6N2E2_2311 overlap by 1 nucleotides Pf6N2E2_2311 and Pf6N2E2_2312 are separated by 18 nucleotides Pf6N2E2_2312 and Pf6N2E2_2313 overlap by 4 nucleotides Pf6N2E2_2313 and Pf6N2E2_2314 are separated by 147 nucleotides Pf6N2E2_2314 and Pf6N2E2_2315 overlap by 1 nucleotides
Pf6N2E2_2310 - Cytochrome c oxidase subunit CcoN (EC 1.9.3.1), at 2,734,242 to 2,735,684
_2310
Pf6N2E2_2311 - Cytochrome c oxidase subunit CcoO (EC 1.9.3.1), at 2,735,684 to 2,736,292
_2311
Pf6N2E2_2312 - Cytochrome c oxidase subunit CcoQ (EC 1.9.3.1), at 2,736,311 to 2,736,484
_2312
Pf6N2E2_2313 - Cytochrome c oxidase subunit CcoP (EC 1.9.3.1), at 2,736,481 to 2,737,464
_2313
Pf6N2E2_2314 - VapB protein (antitoxin to VapC), at 2,737,612 to 2,737,866
_2314
Pf6N2E2_2315 - VapC toxin protein, at 2,737,866 to 2,738,327
_2315
Position (kb)
2736
2737
2738 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2735.505 kb on + strand, within Pf6N2E2_2310 at 2735.505 kb on + strand, within Pf6N2E2_2310 at 2735.506 kb on - strand, within Pf6N2E2_2310 at 2735.601 kb on + strand at 2735.601 kb on + strand at 2735.602 kb on - strand at 2735.602 kb on - strand at 2735.611 kb on - strand at 2735.669 kb on - strand at 2735.701 kb on - strand at 2735.797 kb on + strand, within Pf6N2E2_2311 at 2735.798 kb on - strand, within Pf6N2E2_2311 at 2735.858 kb on + strand at 2735.858 kb on + strand, within Pf6N2E2_2311 at 2735.858 kb on + strand, within Pf6N2E2_2311 at 2735.858 kb on + strand, within Pf6N2E2_2311 at 2735.858 kb on + strand, within Pf6N2E2_2311 at 2735.858 kb on + strand, within Pf6N2E2_2311 at 2735.858 kb on + strand, within Pf6N2E2_2311 at 2735.858 kb on + strand, within Pf6N2E2_2311 at 2735.858 kb on + strand, within Pf6N2E2_2311 at 2735.858 kb on + strand, within Pf6N2E2_2311 at 2735.858 kb on + strand, within Pf6N2E2_2311 at 2735.858 kb on + strand, within Pf6N2E2_2311 at 2735.858 kb on + strand, within Pf6N2E2_2311 at 2735.859 kb on - strand, within Pf6N2E2_2311 at 2735.859 kb on - strand, within Pf6N2E2_2311 at 2735.859 kb on - strand, within Pf6N2E2_2311 at 2735.859 kb on - strand at 2735.859 kb on - strand, within Pf6N2E2_2311 at 2735.859 kb on - strand, within Pf6N2E2_2311 at 2735.927 kb on + strand, within Pf6N2E2_2311 at 2735.927 kb on + strand, within Pf6N2E2_2311 at 2735.937 kb on - strand, within Pf6N2E2_2311 at 2735.992 kb on + strand, within Pf6N2E2_2311 at 2736.056 kb on + strand, within Pf6N2E2_2311 at 2736.056 kb on + strand, within Pf6N2E2_2311 at 2736.056 kb on + strand, within Pf6N2E2_2311 at 2736.056 kb on + strand, within Pf6N2E2_2311 at 2736.056 kb on + strand, within Pf6N2E2_2311 at 2736.056 kb on + strand, within Pf6N2E2_2311 at 2736.057 kb on - strand, within Pf6N2E2_2311 at 2736.179 kb on + strand, within Pf6N2E2_2311 at 2736.466 kb on + strand, within Pf6N2E2_2312 at 2736.466 kb on + strand, within Pf6N2E2_2312 at 2736.466 kb on + strand, within Pf6N2E2_2312 at 2736.628 kb on + strand, within Pf6N2E2_2313 at 2736.745 kb on + strand, within Pf6N2E2_2313 at 2736.745 kb on + strand, within Pf6N2E2_2313 at 2736.751 kb on + strand, within Pf6N2E2_2313 at 2736.752 kb on - strand, within Pf6N2E2_2313 at 2737.301 kb on - strand, within Pf6N2E2_2313 at 2737.301 kb on - strand, within Pf6N2E2_2313 at 2737.411 kb on + strand at 2737.411 kb on + strand at 2737.418 kb on - strand at 2737.418 kb on - strand at 2737.480 kb on + strand at 2737.549 kb on - strand at 2737.549 kb on - strand at 2737.573 kb on - strand at 2737.576 kb on - strand at 2737.584 kb on + strand at 2737.626 kb on + strand at 2737.627 kb on - strand at 2737.731 kb on - strand, within Pf6N2E2_2314 at 2737.745 kb on - strand, within Pf6N2E2_2314 at 2737.745 kb on - strand, within Pf6N2E2_2314 at 2737.869 kb on + strand at 2737.869 kb on + strand at 2737.870 kb on - strand at 2737.870 kb on - strand at 2737.870 kb on - strand at 2737.870 kb on - strand at 2737.870 kb on - strand at 2737.870 kb on - strand at 2737.870 kb on - strand at 2737.883 kb on + strand at 2737.884 kb on - strand at 2737.884 kb on - strand at 2737.884 kb on - strand at 2737.927 kb on + strand, within Pf6N2E2_2315 at 2737.928 kb on - strand, within Pf6N2E2_2315 at 2737.965 kb on + strand, within Pf6N2E2_2315 at 2737.966 kb on - strand, within Pf6N2E2_2315 at 2737.966 kb on - strand, within Pf6N2E2_2315 at 2737.979 kb on + strand, within Pf6N2E2_2315 at 2737.980 kb on - strand, within Pf6N2E2_2315 at 2737.984 kb on + strand, within Pf6N2E2_2315 at 2738.006 kb on - strand, within Pf6N2E2_2315 at 2738.210 kb on - strand, within Pf6N2E2_2315 at 2738.325 kb on + strand at 2738.326 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 5-Hydroxymethylfurfural 10 mM remove 2,735,505 + Pf6N2E2_2310 0.88 -1.4 2,735,505 + Pf6N2E2_2310 0.88 -0.9 2,735,506 - Pf6N2E2_2310 0.88 -2.4 2,735,601 + -2.1 2,735,601 + -1.2 2,735,602 - +1.6 2,735,602 - -1.4 2,735,611 - -2.0 2,735,669 - -0.3 2,735,701 - +0.9 2,735,797 + Pf6N2E2_2311 0.19 -2.8 2,735,798 - Pf6N2E2_2311 0.19 -1.9 2,735,858 + +1.2 2,735,858 + Pf6N2E2_2311 0.29 -1.9 2,735,858 + Pf6N2E2_2311 0.29 -1.7 2,735,858 + Pf6N2E2_2311 0.29 -1.5 2,735,858 + Pf6N2E2_2311 0.29 -2.8 2,735,858 + Pf6N2E2_2311 0.29 -1.2 2,735,858 + Pf6N2E2_2311 0.29 -0.6 2,735,858 + Pf6N2E2_2311 0.29 -3.1 2,735,858 + Pf6N2E2_2311 0.29 -1.2 2,735,858 + Pf6N2E2_2311 0.29 -0.3 2,735,858 + Pf6N2E2_2311 0.29 -1.4 2,735,858 + Pf6N2E2_2311 0.29 -0.3 2,735,858 + Pf6N2E2_2311 0.29 +0.4 2,735,859 - Pf6N2E2_2311 0.29 -0.9 2,735,859 - Pf6N2E2_2311 0.29 +0.9 2,735,859 - Pf6N2E2_2311 0.29 -1.4 2,735,859 - +1.8 2,735,859 - Pf6N2E2_2311 0.29 +0.6 2,735,859 - Pf6N2E2_2311 0.29 -2.7 2,735,927 + Pf6N2E2_2311 0.40 -2.2 2,735,927 + Pf6N2E2_2311 0.40 -1.4 2,735,937 - Pf6N2E2_2311 0.42 -0.4 2,735,992 + Pf6N2E2_2311 0.51 -2.1 2,736,056 + Pf6N2E2_2311 0.61 -2.3 2,736,056 + Pf6N2E2_2311 0.61 +0.2 2,736,056 + Pf6N2E2_2311 0.61 -1.7 2,736,056 + Pf6N2E2_2311 0.61 +0.2 2,736,056 + Pf6N2E2_2311 0.61 -0.0 2,736,056 + Pf6N2E2_2311 0.61 -3.1 2,736,057 - Pf6N2E2_2311 0.61 -2.6 2,736,179 + Pf6N2E2_2311 0.81 -1.0 2,736,466 + Pf6N2E2_2312 0.89 -0.6 2,736,466 + Pf6N2E2_2312 0.89 +0.1 2,736,466 + Pf6N2E2_2312 0.89 -1.2 2,736,628 + Pf6N2E2_2313 0.15 -1.5 2,736,745 + Pf6N2E2_2313 0.27 -1.2 2,736,745 + Pf6N2E2_2313 0.27 -1.4 2,736,751 + Pf6N2E2_2313 0.27 -2.2 2,736,752 - Pf6N2E2_2313 0.28 +0.0 2,737,301 - Pf6N2E2_2313 0.83 -0.8 2,737,301 - Pf6N2E2_2313 0.83 -1.6 2,737,411 + -0.1 2,737,411 + -0.7 2,737,418 - -1.1 2,737,418 - -2.3 2,737,480 + +0.6 2,737,549 - -0.5 2,737,549 - +0.2 2,737,573 - -2.0 2,737,576 - +0.3 2,737,584 + -0.3 2,737,626 + +0.6 2,737,627 - -0.4 2,737,731 - Pf6N2E2_2314 0.47 +0.2 2,737,745 - Pf6N2E2_2314 0.52 +0.5 2,737,745 - Pf6N2E2_2314 0.52 +0.6 2,737,869 + -0.3 2,737,869 + +1.0 2,737,870 - +1.7 2,737,870 - +0.2 2,737,870 - -0.2 2,737,870 - -0.5 2,737,870 - -1.1 2,737,870 - +1.3 2,737,870 - +0.3 2,737,883 + -0.1 2,737,884 - -0.0 2,737,884 - +0.1 2,737,884 - -1.2 2,737,927 + Pf6N2E2_2315 0.13 +0.3 2,737,928 - Pf6N2E2_2315 0.13 -0.5 2,737,965 + Pf6N2E2_2315 0.21 -0.8 2,737,966 - Pf6N2E2_2315 0.22 -1.4 2,737,966 - Pf6N2E2_2315 0.22 -0.5 2,737,979 + Pf6N2E2_2315 0.24 +1.4 2,737,980 - Pf6N2E2_2315 0.25 +0.6 2,737,984 + Pf6N2E2_2315 0.26 +0.5 2,738,006 - Pf6N2E2_2315 0.30 +0.6 2,738,210 - Pf6N2E2_2315 0.74 -1.9 2,738,325 + +1.0 2,738,326 - +1.3
Or see this region's nucleotide sequence