Experiment: 5-Hydroxymethylfurfural 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2073 and Pf6N2E2_2074 overlap by 17 nucleotides Pf6N2E2_2074 and Pf6N2E2_2075 are separated by 158 nucleotides Pf6N2E2_2075 and Pf6N2E2_2076 are separated by 226 nucleotides Pf6N2E2_2076 and Pf6N2E2_2077 are separated by 51 nucleotides
Pf6N2E2_2073 - Sulfite reductase [NADPH] hemoprotein beta-component (EC 1.8.1.2), at 2,466,228 to 2,467,976
_2073
Pf6N2E2_2074 - required for sulfate utilization, putative electron transport protein for sulfite reductase (from data) , at 2,467,960 to 2,468,454
_2074
Pf6N2E2_2075 - Peptidase, U7 family, at 2,468,613 to 2,469,644
_2075
Pf6N2E2_2076 - Phosphoglycerate mutase family protein, at 2,469,871 to 2,470,581
_2076
Pf6N2E2_2077 - SCP-2 sterol transfer family protein, at 2,470,633 to 2,470,947
_2077
Position (kb)
2468
2469
2470 Strain fitness (log2 ratio)
-2
-1
0
1 at 2467.648 kb on - strand, within Pf6N2E2_2073 at 2467.764 kb on + strand, within Pf6N2E2_2073 at 2467.764 kb on + strand, within Pf6N2E2_2073 at 2467.878 kb on + strand at 2467.878 kb on + strand at 2467.878 kb on + strand at 2467.879 kb on - strand at 2467.892 kb on + strand at 2467.892 kb on + strand at 2467.920 kb on + strand at 2467.920 kb on + strand at 2467.920 kb on + strand at 2467.921 kb on - strand at 2467.921 kb on - strand at 2467.921 kb on - strand at 2467.928 kb on + strand at 2467.928 kb on + strand at 2467.928 kb on + strand at 2467.928 kb on + strand at 2467.959 kb on + strand at 2467.959 kb on + strand at 2467.960 kb on - strand at 2468.314 kb on + strand, within Pf6N2E2_2074 at 2468.315 kb on - strand, within Pf6N2E2_2074 at 2468.407 kb on + strand at 2468.407 kb on + strand at 2468.408 kb on - strand at 2468.603 kb on - strand at 2468.603 kb on - strand at 2468.615 kb on - strand at 2468.615 kb on - strand at 2468.723 kb on - strand, within Pf6N2E2_2075 at 2468.735 kb on - strand, within Pf6N2E2_2075 at 2468.873 kb on - strand, within Pf6N2E2_2075 at 2469.214 kb on + strand, within Pf6N2E2_2075 at 2469.215 kb on - strand, within Pf6N2E2_2075 at 2469.255 kb on + strand, within Pf6N2E2_2075 at 2469.256 kb on - strand, within Pf6N2E2_2075 at 2469.256 kb on - strand, within Pf6N2E2_2075 at 2469.256 kb on - strand, within Pf6N2E2_2075 at 2469.256 kb on - strand, within Pf6N2E2_2075 at 2469.256 kb on - strand, within Pf6N2E2_2075 at 2469.256 kb on - strand, within Pf6N2E2_2075 at 2469.256 kb on - strand, within Pf6N2E2_2075 at 2469.480 kb on + strand, within Pf6N2E2_2075 at 2469.481 kb on - strand, within Pf6N2E2_2075 at 2469.491 kb on - strand, within Pf6N2E2_2075 at 2469.656 kb on - strand at 2469.656 kb on - strand at 2469.701 kb on - strand at 2469.742 kb on + strand at 2469.743 kb on - strand at 2469.743 kb on - strand at 2469.743 kb on - strand at 2469.743 kb on - strand at 2469.796 kb on + strand at 2469.797 kb on - strand at 2469.797 kb on - strand at 2469.853 kb on + strand at 2469.854 kb on - strand at 2469.879 kb on + strand at 2469.879 kb on + strand at 2469.880 kb on - strand at 2469.880 kb on - strand at 2469.880 kb on - strand at 2469.880 kb on - strand at 2469.880 kb on - strand at 2469.883 kb on + strand at 2469.883 kb on + strand at 2469.884 kb on - strand at 2469.884 kb on - strand at 2469.884 kb on - strand at 2469.884 kb on - strand at 2469.884 kb on - strand at 2469.884 kb on - strand at 2469.925 kb on + strand at 2469.926 kb on - strand at 2469.926 kb on - strand at 2469.998 kb on + strand, within Pf6N2E2_2076 at 2470.590 kb on + strand at 2470.595 kb on + strand at 2470.596 kb on - strand at 2470.609 kb on - strand at 2470.609 kb on - strand at 2470.609 kb on - strand at 2470.609 kb on - strand at 2470.609 kb on - strand at 2470.609 kb on - strand at 2470.619 kb on + strand at 2470.620 kb on - strand at 2470.620 kb on - strand at 2470.620 kb on - strand at 2470.620 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 5-Hydroxymethylfurfural 10 mM remove 2,467,648 - Pf6N2E2_2073 0.81 -1.2 2,467,764 + Pf6N2E2_2073 0.88 +0.7 2,467,764 + Pf6N2E2_2073 0.88 +0.2 2,467,878 + -1.2 2,467,878 + +0.8 2,467,878 + -0.2 2,467,879 - -1.6 2,467,892 + +0.2 2,467,892 + -0.5 2,467,920 + +0.3 2,467,920 + +0.9 2,467,920 + +0.8 2,467,921 - -0.8 2,467,921 - +0.1 2,467,921 - -0.3 2,467,928 + -0.1 2,467,928 + -1.5 2,467,928 + +0.0 2,467,928 + +0.5 2,467,959 + -0.2 2,467,959 + +1.2 2,467,960 - +1.2 2,468,314 + Pf6N2E2_2074 0.72 +0.3 2,468,315 - Pf6N2E2_2074 0.72 +0.5 2,468,407 + +1.3 2,468,407 + +0.6 2,468,408 - -0.1 2,468,603 - +0.3 2,468,603 - -2.3 2,468,615 - -0.0 2,468,615 - +0.2 2,468,723 - Pf6N2E2_2075 0.11 +0.5 2,468,735 - Pf6N2E2_2075 0.12 -0.3 2,468,873 - Pf6N2E2_2075 0.25 -0.6 2,469,214 + Pf6N2E2_2075 0.58 -0.1 2,469,215 - Pf6N2E2_2075 0.58 +0.3 2,469,255 + Pf6N2E2_2075 0.62 -0.3 2,469,256 - Pf6N2E2_2075 0.62 +1.5 2,469,256 - Pf6N2E2_2075 0.62 +0.5 2,469,256 - Pf6N2E2_2075 0.62 +0.1 2,469,256 - Pf6N2E2_2075 0.62 +0.6 2,469,256 - Pf6N2E2_2075 0.62 -0.2 2,469,256 - Pf6N2E2_2075 0.62 -0.8 2,469,256 - Pf6N2E2_2075 0.62 -0.2 2,469,480 + Pf6N2E2_2075 0.84 -0.5 2,469,481 - Pf6N2E2_2075 0.84 +0.0 2,469,491 - Pf6N2E2_2075 0.85 -0.2 2,469,656 - +0.2 2,469,656 - +0.1 2,469,701 - -0.4 2,469,742 + +0.9 2,469,743 - -0.3 2,469,743 - +0.0 2,469,743 - -0.8 2,469,743 - +0.1 2,469,796 + +0.1 2,469,797 - -0.4 2,469,797 - -0.4 2,469,853 + +0.9 2,469,854 - -2.0 2,469,879 + +0.1 2,469,879 + +0.1 2,469,880 - -0.1 2,469,880 - -0.1 2,469,880 - -0.4 2,469,880 - -2.1 2,469,880 - +0.1 2,469,883 + +0.4 2,469,883 + -0.7 2,469,884 - -2.2 2,469,884 - +0.9 2,469,884 - -1.5 2,469,884 - -1.1 2,469,884 - -1.9 2,469,884 - +0.2 2,469,925 + +0.4 2,469,926 - +0.2 2,469,926 - +0.3 2,469,998 + Pf6N2E2_2076 0.18 +0.0 2,470,590 + +0.7 2,470,595 + -0.2 2,470,596 - -0.8 2,470,609 - -0.3 2,470,609 - -0.9 2,470,609 - -1.0 2,470,609 - -1.6 2,470,609 - -0.5 2,470,609 - -0.7 2,470,619 + -0.0 2,470,620 - +0.2 2,470,620 - -0.5 2,470,620 - -2.5 2,470,620 - +0.1
Or see this region's nucleotide sequence