Experiment: 5-Hydroxymethylfurfural 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1623 and Pf6N2E2_1624 are separated by 36 nucleotides Pf6N2E2_1624 and Pf6N2E2_1625 are separated by 203 nucleotides Pf6N2E2_1625 and Pf6N2E2_1626 are separated by 7 nucleotides
Pf6N2E2_1623 - Siderophore biosynthesis diaminobutyrate--2-oxoglutarate aminotransferase (EC 2.6.1.76), at 1,899,027 to 1,900,457
_1623
Pf6N2E2_1624 - MbtH-like protein, at 1,900,494 to 1,900,709
_1624
Pf6N2E2_1625 - Ferric reductase (1.6.99.14), at 1,900,913 to 1,901,650
_1625
Pf6N2E2_1626 - Putative ATP-binding component of a transport system, at 1,901,658 to 1,903,301
_1626
Position (kb)
1900
1901
1902 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1900.276 kb on + strand, within Pf6N2E2_1623 at 1900.276 kb on + strand, within Pf6N2E2_1623 at 1900.277 kb on - strand, within Pf6N2E2_1623 at 1900.277 kb on - strand, within Pf6N2E2_1623 at 1900.434 kb on + strand at 1900.434 kb on + strand at 1900.435 kb on - strand at 1900.435 kb on - strand at 1900.473 kb on + strand at 1900.509 kb on + strand at 1900.509 kb on + strand at 1900.510 kb on - strand at 1900.510 kb on - strand at 1900.567 kb on + strand, within Pf6N2E2_1624 at 1900.567 kb on + strand, within Pf6N2E2_1624 at 1900.568 kb on - strand, within Pf6N2E2_1624 at 1900.568 kb on - strand, within Pf6N2E2_1624 at 1900.672 kb on - strand, within Pf6N2E2_1624 at 1900.672 kb on - strand, within Pf6N2E2_1624 at 1900.751 kb on - strand at 1900.855 kb on - strand at 1900.855 kb on - strand at 1900.856 kb on + strand at 1900.856 kb on + strand at 1900.857 kb on - strand at 1900.857 kb on - strand at 1901.001 kb on + strand, within Pf6N2E2_1625 at 1901.031 kb on - strand, within Pf6N2E2_1625 at 1901.291 kb on - strand, within Pf6N2E2_1625 at 1901.383 kb on + strand, within Pf6N2E2_1625 at 1901.384 kb on - strand, within Pf6N2E2_1625 at 1901.648 kb on + strand at 1901.648 kb on + strand at 1901.659 kb on + strand at 1901.698 kb on + strand at 1901.716 kb on + strand at 1901.729 kb on - strand at 1901.753 kb on - strand at 1901.821 kb on + strand at 1901.821 kb on + strand at 1901.822 kb on - strand at 1901.905 kb on - strand, within Pf6N2E2_1626 at 1902.172 kb on + strand, within Pf6N2E2_1626 at 1902.172 kb on + strand, within Pf6N2E2_1626 at 1902.173 kb on - strand, within Pf6N2E2_1626 at 1902.173 kb on - strand, within Pf6N2E2_1626 at 1902.499 kb on + strand, within Pf6N2E2_1626 at 1902.499 kb on + strand, within Pf6N2E2_1626 at 1902.558 kb on - strand, within Pf6N2E2_1626 at 1902.567 kb on + strand, within Pf6N2E2_1626
Per-strain Table
Position Strand Gene LocusTag Fraction 5-Hydroxymethylfurfural 10 mM remove 1,900,276 + Pf6N2E2_1623 0.87 +0.9 1,900,276 + Pf6N2E2_1623 0.87 -1.0 1,900,277 - Pf6N2E2_1623 0.87 -0.5 1,900,277 - Pf6N2E2_1623 0.87 +0.4 1,900,434 + +0.5 1,900,434 + +0.0 1,900,435 - +1.3 1,900,435 - -0.6 1,900,473 + -0.2 1,900,509 + +0.5 1,900,509 + -0.6 1,900,510 - -0.4 1,900,510 - +0.5 1,900,567 + Pf6N2E2_1624 0.34 +0.3 1,900,567 + Pf6N2E2_1624 0.34 +0.4 1,900,568 - Pf6N2E2_1624 0.34 +0.9 1,900,568 - Pf6N2E2_1624 0.34 +0.3 1,900,672 - Pf6N2E2_1624 0.82 -0.4 1,900,672 - Pf6N2E2_1624 0.82 -0.3 1,900,751 - -0.4 1,900,855 - +1.1 1,900,855 - -1.5 1,900,856 + -0.5 1,900,856 + +0.5 1,900,857 - -2.5 1,900,857 - +0.3 1,901,001 + Pf6N2E2_1625 0.12 +0.0 1,901,031 - Pf6N2E2_1625 0.16 -0.4 1,901,291 - Pf6N2E2_1625 0.51 -1.9 1,901,383 + Pf6N2E2_1625 0.64 -2.0 1,901,384 - Pf6N2E2_1625 0.64 -1.5 1,901,648 + +0.2 1,901,648 + +0.5 1,901,659 + +0.5 1,901,698 + -0.5 1,901,716 + +0.2 1,901,729 - -3.0 1,901,753 - -0.3 1,901,821 + +0.3 1,901,821 + -0.1 1,901,822 - -1.5 1,901,905 - Pf6N2E2_1626 0.15 -0.4 1,902,172 + Pf6N2E2_1626 0.31 -1.0 1,902,172 + Pf6N2E2_1626 0.31 -0.1 1,902,173 - Pf6N2E2_1626 0.31 -0.6 1,902,173 - Pf6N2E2_1626 0.31 -0.6 1,902,499 + Pf6N2E2_1626 0.51 -1.0 1,902,499 + Pf6N2E2_1626 0.51 +0.4 1,902,558 - Pf6N2E2_1626 0.55 -1.7 1,902,567 + Pf6N2E2_1626 0.55 +0.1
Or see this region's nucleotide sequence