Experiment: H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS03015 and MMP_RS03020 are separated by 131 nucleotides MMP_RS03020 and MMP_RS03025 are separated by 110 nucleotides MMP_RS03025 and MMP_RS03030 are separated by 541 nucleotides MMP_RS03030 and MMP_RS03035 are separated by 17 nucleotides
MMP_RS03015: MMP_RS03015 - GTP 3' 8-cyclase MoaA, at 570,696 to 571,589
_RS03015
MMP_RS03020: MMP_RS03020 - peptidylprolyl isomerase, at 571,721 to 572,407
_RS03020
MMP_RS03025: MMP_RS03025 - molybdenum cofactor guanylyltransferase, at 572,518 to 573,162
_RS03025
MMP_RS03030: MMP_RS03030 - Asp-tRNA(Asn) amidotransferase subunit GatC, at 573,704 to 573,943
_RS03030
MMP_RS03035: MMP_RS03035 - 4-hydroxy-tetrahydrodipicolinate synthase, at 573,961 to 574,830
_RS03035
Position (kb) 572
573
574 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 571.668 kb on + strand at 571.709 kb on - strand at 571.723 kb on + strand at 571.863 kb on + strand, within MMP_RS03020 at 571.863 kb on + strand, within MMP_RS03020 at 571.863 kb on + strand, within MMP_RS03020 at 571.868 kb on - strand, within MMP_RS03020 at 571.941 kb on + strand, within MMP_RS03020 at 571.964 kb on - strand, within MMP_RS03020 at 572.054 kb on - strand, within MMP_RS03020 at 572.430 kb on - strand at 572.461 kb on - strand at 572.461 kb on - strand at 572.479 kb on - strand at 572.479 kb on - strand at 572.499 kb on + strand at 572.518 kb on + strand at 572.532 kb on - strand at 572.533 kb on + strand at 572.538 kb on + strand at 572.604 kb on + strand, within MMP_RS03025 at 572.614 kb on + strand, within MMP_RS03025 at 572.714 kb on + strand, within MMP_RS03025 at 572.716 kb on - strand, within MMP_RS03025 at 572.742 kb on - strand, within MMP_RS03025 at 572.867 kb on - strand, within MMP_RS03025 at 572.900 kb on - strand, within MMP_RS03025 at 572.901 kb on + strand, within MMP_RS03025 at 572.918 kb on - strand, within MMP_RS03025 at 572.918 kb on - strand, within MMP_RS03025 at 572.919 kb on + strand, within MMP_RS03025 at 572.919 kb on + strand, within MMP_RS03025 at 572.944 kb on + strand, within MMP_RS03025 at 572.965 kb on - strand, within MMP_RS03025 at 573.107 kb on - strand at 573.107 kb on - strand at 573.107 kb on - strand at 573.111 kb on - strand at 573.128 kb on - strand at 573.172 kb on - strand at 573.193 kb on + strand at 573.193 kb on + strand at 573.200 kb on + strand at 573.200 kb on + strand at 573.200 kb on + strand at 573.228 kb on - strand at 573.260 kb on - strand at 573.290 kb on - strand at 573.309 kb on - strand at 573.310 kb on + strand at 573.310 kb on + strand at 573.350 kb on + strand at 573.354 kb on - strand at 573.381 kb on - strand at 573.396 kb on - strand at 573.396 kb on - strand at 573.396 kb on - strand at 573.424 kb on - strand at 573.425 kb on + strand at 573.425 kb on + strand at 573.425 kb on + strand at 573.426 kb on - strand at 573.426 kb on - strand at 573.427 kb on + strand at 573.428 kb on - strand at 573.429 kb on + strand at 573.526 kb on - strand at 573.527 kb on + strand at 573.562 kb on + strand at 573.565 kb on - strand at 573.565 kb on - strand at 573.565 kb on - strand at 573.566 kb on + strand at 573.566 kb on + strand at 573.604 kb on + strand at 573.754 kb on - strand at 573.943 kb on - strand at 574.069 kb on + strand, within MMP_RS03035
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2 remove 571,668 + +0.3 571,709 - +0.2 571,723 + -0.4 571,863 + MMP_RS03020 0.21 -0.3 571,863 + MMP_RS03020 0.21 -0.8 571,863 + MMP_RS03020 0.21 -1.6 571,868 - MMP_RS03020 0.21 -0.7 571,941 + MMP_RS03020 0.32 -0.7 571,964 - MMP_RS03020 0.35 +0.4 572,054 - MMP_RS03020 0.48 -0.6 572,430 - -0.1 572,461 - -1.2 572,461 - -0.4 572,479 - -0.9 572,479 - -1.5 572,499 + -0.5 572,518 + -0.9 572,532 - -2.1 572,533 + -2.0 572,538 + -2.2 572,604 + MMP_RS03025 0.13 -1.5 572,614 + MMP_RS03025 0.15 -1.9 572,714 + MMP_RS03025 0.30 -1.7 572,716 - MMP_RS03025 0.31 -1.8 572,742 - MMP_RS03025 0.35 -0.7 572,867 - MMP_RS03025 0.54 -1.4 572,900 - MMP_RS03025 0.59 -1.7 572,901 + MMP_RS03025 0.59 -1.0 572,918 - MMP_RS03025 0.62 -1.0 572,918 - MMP_RS03025 0.62 -3.7 572,919 + MMP_RS03025 0.62 -3.7 572,919 + MMP_RS03025 0.62 -0.4 572,944 + MMP_RS03025 0.66 -1.6 572,965 - MMP_RS03025 0.69 -1.6 573,107 - -1.8 573,107 - -1.2 573,107 - -1.4 573,111 - -3.2 573,128 - -1.7 573,172 - -0.3 573,193 + -0.3 573,193 + -0.1 573,200 + -0.4 573,200 + -0.2 573,200 + +0.6 573,228 - -0.2 573,260 - +0.2 573,290 - -0.2 573,309 - +0.1 573,310 + -0.8 573,310 + -0.6 573,350 + -0.2 573,354 - -0.1 573,381 - -0.1 573,396 - -0.1 573,396 - +0.0 573,396 - +0.2 573,424 - +0.2 573,425 + +0.1 573,425 + +0.4 573,425 + +0.3 573,426 - -0.1 573,426 - +0.2 573,427 + +0.6 573,428 - +0.3 573,429 + -0.4 573,526 - +0.4 573,527 + -0.1 573,562 + -0.4 573,565 - -0.6 573,565 - -1.4 573,565 - -0.5 573,566 + +0.0 573,566 + -0.3 573,604 + -2.8 573,754 - -2.8 573,943 - -1.8 574,069 + MMP_RS03035 0.12 -1.7
Or see this region's nucleotide sequence