Strain Fitness in Methanococcus maripaludis S2 around MMP_RS02190

Experiment: H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMMP_RS02185 and MMP_RS02190 are separated by 70 nucleotidesMMP_RS02190 and MMP_RS02195 are separated by 34 nucleotides MMP_RS02185: MMP_RS02185 - ribose-phosphate diphosphokinase, at 408,933 to 409,796 _RS02185 MMP_RS02190: MMP_RS02190 - sulfopyruvate decarboxylase subunit alpha, at 409,867 to 410,370 _RS02190 MMP_RS02195: MMP_RS02195 - tRNA (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-methylthiotransferase, at 410,405 to 411,682 _RS02195 Position (kb) 409 410 411Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 408.887 kb on - strandat 409.043 kb on + strandat 409.045 kb on + strandat 409.088 kb on + strandat 409.769 kb on + strandat 409.798 kb on - strandat 409.798 kb on - strandat 409.903 kb on - strandat 409.930 kb on + strand, within MMP_RS02190at 410.106 kb on - strand, within MMP_RS02190at 410.274 kb on + strand, within MMP_RS02190at 410.349 kb on + strandat 410.371 kb on + strandat 410.527 kb on + strandat 410.622 kb on - strand, within MMP_RS02195at 410.845 kb on - strand, within MMP_RS02195at 410.872 kb on - strand, within MMP_RS02195at 410.884 kb on + strand, within MMP_RS02195at 411.366 kb on - strand, within MMP_RS02195

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Per-strain Table

Position Strand Gene LocusTag Fraction H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2
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408,887 - -2.5
409,043 + -2.7
409,045 + -3.0
409,088 + -2.8
409,769 + -0.5
409,798 - -2.5
409,798 - -3.1
409,903 - -2.2
409,930 + MMP_RS02190 0.12 -2.2
410,106 - MMP_RS02190 0.47 -2.8
410,274 + MMP_RS02190 0.81 -2.0
410,349 + -1.0
410,371 + -1.1
410,527 + -1.1
410,622 - MMP_RS02195 0.17 -3.6
410,845 - MMP_RS02195 0.34 -1.8
410,872 - MMP_RS02195 0.37 -3.8
410,884 + MMP_RS02195 0.37 -2.3
411,366 - MMP_RS02195 0.75 -2.9

Or see this region's nucleotide sequence