Experiment: H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS02120 and MMP_RS02125 are separated by 41 nucleotides MMP_RS02125 and MMP_RS02130 are separated by 10 nucleotides MMP_RS02130 and MMP_RS02135 are separated by 9 nucleotides MMP_RS02135 and MMP_RS02140 are separated by 35 nucleotides
MMP_RS02120: MMP_RS02120 - alanine--tRNA ligase, at 394,599 to 397,277
_RS02120
MMP_RS02125: MMP_RS02125 - pantoate kinase, at 397,319 to 398,191
_RS02125
MMP_RS02130: MMP_RS02130 - zinc metalloprotease HtpX, at 398,202 to 399,059
_RS02130
MMP_RS02135: MMP_RS02135 - DUF5612 domain-containing protein, at 399,069 to 399,722
_RS02135
MMP_RS02140: MMP_RS02140 - methionine synthase, at 399,758 to 400,696
_RS02140
Position (kb) 398
399
400 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 397.309 kb on + strand at 397.336 kb on - strand at 397.361 kb on + strand at 397.870 kb on - strand at 397.871 kb on + strand at 397.872 kb on - strand at 397.873 kb on + strand at 397.900 kb on + strand at 397.998 kb on - strand at 397.998 kb on - strand at 398.136 kb on - strand at 398.190 kb on + strand at 398.211 kb on + strand at 398.268 kb on - strand at 398.269 kb on + strand at 398.271 kb on + strand at 398.271 kb on + strand at 398.271 kb on + strand at 398.300 kb on + strand, within MMP_RS02130 at 398.323 kb on - strand, within MMP_RS02130 at 398.324 kb on + strand, within MMP_RS02130 at 398.351 kb on + strand, within MMP_RS02130 at 398.364 kb on - strand, within MMP_RS02130 at 398.365 kb on + strand, within MMP_RS02130 at 398.365 kb on + strand, within MMP_RS02130 at 398.378 kb on + strand, within MMP_RS02130 at 398.522 kb on + strand, within MMP_RS02130 at 398.535 kb on + strand, within MMP_RS02130 at 398.602 kb on - strand, within MMP_RS02130 at 398.603 kb on + strand, within MMP_RS02130 at 398.617 kb on - strand, within MMP_RS02130 at 398.617 kb on - strand, within MMP_RS02130 at 398.618 kb on + strand, within MMP_RS02130 at 398.632 kb on - strand, within MMP_RS02130 at 398.661 kb on - strand, within MMP_RS02130 at 398.661 kb on - strand, within MMP_RS02130 at 398.662 kb on + strand, within MMP_RS02130 at 398.856 kb on - strand, within MMP_RS02130 at 399.089 kb on + strand at 399.119 kb on - strand at 399.158 kb on + strand, within MMP_RS02135 at 399.162 kb on - strand, within MMP_RS02135 at 399.162 kb on - strand, within MMP_RS02135 at 399.163 kb on + strand, within MMP_RS02135 at 399.192 kb on + strand, within MMP_RS02135 at 399.207 kb on + strand, within MMP_RS02135 at 399.423 kb on + strand, within MMP_RS02135 at 399.587 kb on + strand, within MMP_RS02135 at 399.694 kb on - strand at 399.695 kb on + strand at 399.789 kb on - strand at 399.850 kb on - strand at 399.948 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2 remove 397,309 + -2.1 397,336 - -2.7 397,361 + -2.1 397,870 - -2.4 397,871 + -3.5 397,872 - -2.6 397,873 + -2.4 397,900 + -1.2 397,998 - -4.2 397,998 - -2.3 398,136 - -2.1 398,190 + -0.8 398,211 + -0.2 398,268 - +0.2 398,269 + +0.1 398,271 + -0.1 398,271 + +0.2 398,271 + +0.0 398,300 + MMP_RS02130 0.11 -0.0 398,323 - MMP_RS02130 0.14 -0.0 398,324 + MMP_RS02130 0.14 +0.0 398,351 + MMP_RS02130 0.17 -0.1 398,364 - MMP_RS02130 0.19 -0.1 398,365 + MMP_RS02130 0.19 -0.4 398,365 + MMP_RS02130 0.19 +0.1 398,378 + MMP_RS02130 0.21 +0.0 398,522 + MMP_RS02130 0.37 -2.1 398,535 + MMP_RS02130 0.39 -1.6 398,602 - MMP_RS02130 0.47 -3.0 398,603 + MMP_RS02130 0.47 -1.1 398,617 - MMP_RS02130 0.48 -0.6 398,617 - MMP_RS02130 0.48 -2.6 398,618 + MMP_RS02130 0.48 -0.4 398,632 - MMP_RS02130 0.50 -2.4 398,661 - MMP_RS02130 0.53 -1.8 398,661 - MMP_RS02130 0.53 -2.1 398,662 + MMP_RS02130 0.54 -0.5 398,856 - MMP_RS02130 0.76 -3.7 399,089 + -1.3 399,119 - -2.7 399,158 + MMP_RS02135 0.14 -2.3 399,162 - MMP_RS02135 0.14 -2.2 399,162 - MMP_RS02135 0.14 -2.7 399,163 + MMP_RS02135 0.14 -2.2 399,192 + MMP_RS02135 0.19 -1.4 399,207 + MMP_RS02135 0.21 -1.9 399,423 + MMP_RS02135 0.54 -1.4 399,587 + MMP_RS02135 0.79 -2.2 399,694 - -2.7 399,695 + -2.2 399,789 - -2.9 399,850 - -2.6 399,948 - -2.8
Or see this region's nucleotide sequence