Experiment: H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS00715 and MMP_RS00720 are separated by 27 nucleotides MMP_RS00720 and MMP_RS00725 are separated by 40 nucleotides MMP_RS00725 and MMP_RS00730 are separated by 28 nucleotides
MMP_RS00715: MMP_RS00715 - formate-dependent phosphoribosylglycinamide formyltransferase, at 129,070 to 130,236
_RS00715
MMP_RS00720: MMP_RS00720 - metal-dependent hydrolase, at 130,264 to 130,938
_RS00720
MMP_RS00725: MMP_RS00725 - 5 10-methenyltetrahydromethanopterin hydrogenase cofactor biosynthesis protein HmdC, at 130,979 to 132,511
_RS00725
MMP_RS00730: MMP_RS00730 - 5 10-methenyltetrahydromethanopterin hydrogenase cofactor biosynthesis protein HmdB, at 132,540 to 133,565
_RS00730
Position (kb) 130
131
132
133 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 130.010 kb on - strand at 130.115 kb on - strand at 130.146 kb on - strand at 130.284 kb on + strand at 130.292 kb on + strand at 130.477 kb on + strand, within MMP_RS00720 at 130.534 kb on + strand, within MMP_RS00720 at 130.650 kb on - strand, within MMP_RS00720 at 130.683 kb on - strand, within MMP_RS00720 at 130.684 kb on + strand, within MMP_RS00720 at 130.685 kb on - strand, within MMP_RS00720 at 130.748 kb on + strand, within MMP_RS00720 at 130.751 kb on - strand, within MMP_RS00720 at 130.752 kb on + strand, within MMP_RS00720 at 130.773 kb on - strand, within MMP_RS00720 at 130.790 kb on - strand, within MMP_RS00720 at 130.791 kb on + strand, within MMP_RS00720 at 130.807 kb on - strand, within MMP_RS00720 at 130.808 kb on + strand, within MMP_RS00720 at 130.819 kb on - strand, within MMP_RS00720 at 130.831 kb on - strand, within MMP_RS00720 at 130.887 kb on - strand at 130.907 kb on + strand at 130.918 kb on + strand at 130.986 kb on + strand at 131.041 kb on - strand at 131.092 kb on - strand at 131.101 kb on + strand at 131.101 kb on + strand at 131.101 kb on + strand at 131.123 kb on - strand at 131.173 kb on + strand, within MMP_RS00725 at 131.173 kb on + strand, within MMP_RS00725 at 131.174 kb on - strand, within MMP_RS00725 at 131.174 kb on - strand, within MMP_RS00725 at 131.175 kb on + strand, within MMP_RS00725 at 131.612 kb on + strand, within MMP_RS00725 at 131.657 kb on + strand, within MMP_RS00725 at 131.738 kb on - strand, within MMP_RS00725 at 131.786 kb on - strand, within MMP_RS00725 at 132.136 kb on - strand, within MMP_RS00725 at 132.392 kb on - strand at 132.439 kb on - strand at 132.582 kb on - strand at 132.583 kb on + strand at 132.583 kb on + strand at 132.583 kb on + strand at 132.583 kb on + strand at 132.899 kb on - strand, within MMP_RS00730 at 132.942 kb on - strand, within MMP_RS00730 at 133.070 kb on - strand, within MMP_RS00730 at 133.104 kb on - strand, within MMP_RS00730 at 133.174 kb on + strand, within MMP_RS00730 at 133.288 kb on + strand, within MMP_RS00730 at 133.362 kb on - strand, within MMP_RS00730 at 133.441 kb on - strand, within MMP_RS00730 at 133.490 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2 remove 130,010 - -2.4 130,115 - -2.3 130,146 - -2.5 130,284 + -0.1 130,292 + -0.8 130,477 + MMP_RS00720 0.32 -0.8 130,534 + MMP_RS00720 0.40 -0.5 130,650 - MMP_RS00720 0.57 -0.9 130,683 - MMP_RS00720 0.62 -0.8 130,684 + MMP_RS00720 0.62 -1.1 130,685 - MMP_RS00720 0.62 -0.7 130,748 + MMP_RS00720 0.72 -1.2 130,751 - MMP_RS00720 0.72 -1.4 130,752 + MMP_RS00720 0.72 -1.8 130,773 - MMP_RS00720 0.75 -0.3 130,790 - MMP_RS00720 0.78 -0.4 130,791 + MMP_RS00720 0.78 -1.5 130,807 - MMP_RS00720 0.80 -1.1 130,808 + MMP_RS00720 0.81 -1.1 130,819 - MMP_RS00720 0.82 -0.6 130,831 - MMP_RS00720 0.84 -1.5 130,887 - -0.9 130,907 + -0.7 130,918 + -1.0 130,986 + -0.2 131,041 - -1.8 131,092 - -3.1 131,101 + -1.8 131,101 + -2.1 131,101 + -1.1 131,123 - -1.9 131,173 + MMP_RS00725 0.13 -1.8 131,173 + MMP_RS00725 0.13 -1.5 131,174 - MMP_RS00725 0.13 -1.6 131,174 - MMP_RS00725 0.13 -3.8 131,175 + MMP_RS00725 0.13 -1.1 131,612 + MMP_RS00725 0.41 -1.2 131,657 + MMP_RS00725 0.44 -1.3 131,738 - MMP_RS00725 0.50 -1.9 131,786 - MMP_RS00725 0.53 -1.2 132,136 - MMP_RS00725 0.75 -1.6 132,392 - -1.2 132,439 - -1.5 132,582 - -1.0 132,583 + -1.7 132,583 + -1.9 132,583 + -1.8 132,583 + -1.2 132,899 - MMP_RS00730 0.35 -1.8 132,942 - MMP_RS00730 0.39 -1.4 133,070 - MMP_RS00730 0.52 -0.8 133,104 - MMP_RS00730 0.55 -1.4 133,174 + MMP_RS00730 0.62 -3.3 133,288 + MMP_RS00730 0.73 -1.7 133,362 - MMP_RS00730 0.80 -1.2 133,441 - MMP_RS00730 0.88 -1.6 133,490 - -1.3
Or see this region's nucleotide sequence