Experiment: H2 N-free with 2g/L casaminos
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS07715 and MMJJ_RS07720 are separated by 39 nucleotides MMJJ_RS07720 and MMJJ_RS07725 are separated by 33 nucleotides MMJJ_RS07725 and MMJJ_RS07730 are separated by 233 nucleotides MMJJ_RS07730 and MMJJ_RS07735 are separated by 18 nucleotides MMJJ_RS07735 and MMJJ_RS07740 are separated by 120 nucleotides
MMJJ_RS07715: MMJJ_RS07715 - UbiD family decarboxylase, at 1,415,721 to 1,416,998
_RS07715
MMJJ_RS07720: MMJJ_RS07720 - UPF0058 family protein, at 1,417,038 to 1,417,331
_RS07720
MMJJ_RS07725: MMJJ_RS07725 - DNA primase DnaG, at 1,417,365 to 1,418,675
_RS07725
MMJJ_RS07730: MMJJ_RS07730 - tRNA-Gly, at 1,418,909 to 1,418,981
_RS07730
MMJJ_RS07735: MMJJ_RS07735 - tRNA-Gly, at 1,419,000 to 1,419,072
_RS07735
MMJJ_RS07740: MMJJ_RS07740 - DUF2334 domain-containing protein, at 1,419,193 to 1,419,993
_RS07740
Position (kb)
1417
1418
1419 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1416.607 kb on + strand, within MMJJ_RS07715 at 1416.637 kb on + strand, within MMJJ_RS07715 at 1416.677 kb on + strand, within MMJJ_RS07715 at 1416.802 kb on + strand, within MMJJ_RS07715 at 1417.159 kb on + strand, within MMJJ_RS07720 at 1417.175 kb on - strand, within MMJJ_RS07720 at 1417.175 kb on - strand, within MMJJ_RS07720 at 1417.287 kb on + strand, within MMJJ_RS07720 at 1417.306 kb on + strand at 1417.485 kb on - strand at 1417.620 kb on - strand, within MMJJ_RS07725 at 1417.667 kb on - strand, within MMJJ_RS07725 at 1417.680 kb on - strand, within MMJJ_RS07725 at 1417.680 kb on - strand, within MMJJ_RS07725 at 1417.682 kb on - strand, within MMJJ_RS07725 at 1417.683 kb on + strand, within MMJJ_RS07725 at 1417.704 kb on - strand at 1417.714 kb on + strand at 1417.716 kb on - strand, within MMJJ_RS07725 at 1417.716 kb on - strand, within MMJJ_RS07725 at 1417.718 kb on - strand, within MMJJ_RS07725 at 1417.719 kb on + strand, within MMJJ_RS07725 at 1417.833 kb on - strand, within MMJJ_RS07725 at 1417.853 kb on - strand, within MMJJ_RS07725 at 1417.908 kb on - strand, within MMJJ_RS07725 at 1418.101 kb on + strand, within MMJJ_RS07725 at 1418.124 kb on - strand at 1418.162 kb on - strand, within MMJJ_RS07725 at 1418.372 kb on - strand, within MMJJ_RS07725 at 1418.645 kb on + strand at 1418.651 kb on - strand at 1418.676 kb on + strand at 1418.737 kb on - strand at 1418.752 kb on - strand at 1418.753 kb on + strand at 1418.753 kb on + strand at 1418.756 kb on - strand at 1418.760 kb on - strand at 1418.760 kb on - strand at 1418.761 kb on + strand at 1418.766 kb on + strand at 1418.766 kb on + strand at 1418.774 kb on - strand at 1418.774 kb on - strand at 1418.775 kb on + strand at 1418.775 kb on + strand at 1418.775 kb on + strand at 1418.776 kb on - strand at 1418.776 kb on - strand at 1418.777 kb on + strand at 1418.777 kb on + strand at 1418.777 kb on + strand at 1418.778 kb on - strand at 1418.778 kb on - strand at 1418.778 kb on - strand at 1418.778 kb on - strand at 1418.778 kb on - strand at 1418.778 kb on - strand at 1418.778 kb on - strand at 1418.779 kb on + strand at 1418.780 kb on - strand at 1418.780 kb on - strand at 1418.781 kb on + strand at 1418.784 kb on - strand at 1418.784 kb on - strand at 1418.784 kb on - strand at 1418.785 kb on + strand at 1418.785 kb on + strand at 1418.787 kb on - strand at 1418.787 kb on - strand at 1418.787 kb on - strand at 1418.823 kb on - strand at 1419.044 kb on - strand, within MMJJ_RS07735 at 1419.080 kb on + strand at 1419.080 kb on + strand at 1419.129 kb on - strand at 1419.129 kb on - strand at 1419.129 kb on - strand at 1419.130 kb on + strand at 1419.130 kb on + strand at 1419.130 kb on + strand at 1419.130 kb on + strand at 1419.130 kb on + strand at 1419.131 kb on - strand at 1419.131 kb on - strand at 1419.131 kb on - strand at 1419.131 kb on - strand at 1419.132 kb on + strand at 1419.132 kb on + strand at 1419.132 kb on + strand at 1419.132 kb on + strand at 1419.132 kb on + strand at 1419.132 kb on + strand at 1419.132 kb on + strand at 1419.133 kb on - strand at 1419.133 kb on - strand at 1419.133 kb on - strand at 1419.133 kb on - strand at 1419.148 kb on - strand at 1419.149 kb on + strand at 1419.158 kb on - strand at 1419.159 kb on + strand at 1419.162 kb on - strand at 1419.163 kb on + strand at 1419.165 kb on + strand at 1419.175 kb on + strand at 1419.184 kb on - strand at 1419.184 kb on - strand at 1419.185 kb on + strand at 1419.185 kb on + strand at 1419.228 kb on + strand at 1419.319 kb on + strand, within MMJJ_RS07740 at 1419.328 kb on - strand, within MMJJ_RS07740 at 1419.359 kb on - strand, within MMJJ_RS07740 at 1419.359 kb on - strand, within MMJJ_RS07740 at 1419.360 kb on + strand, within MMJJ_RS07740 at 1419.364 kb on + strand, within MMJJ_RS07740 at 1419.370 kb on - strand, within MMJJ_RS07740 at 1419.371 kb on + strand, within MMJJ_RS07740 at 1419.425 kb on + strand, within MMJJ_RS07740 at 1419.484 kb on - strand, within MMJJ_RS07740 at 1419.485 kb on + strand, within MMJJ_RS07740 at 1419.534 kb on - strand, within MMJJ_RS07740 at 1419.534 kb on - strand, within MMJJ_RS07740 at 1419.613 kb on + strand, within MMJJ_RS07740
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 2g/L casaminos remove 1,416,607 + MMJJ_RS07715 0.69 -2.1 1,416,637 + MMJJ_RS07715 0.72 -4.3 1,416,677 + MMJJ_RS07715 0.75 -0.6 1,416,802 + MMJJ_RS07715 0.85 +1.1 1,417,159 + MMJJ_RS07720 0.41 -0.9 1,417,175 - MMJJ_RS07720 0.47 -2.3 1,417,175 - MMJJ_RS07720 0.47 -4.1 1,417,287 + MMJJ_RS07720 0.85 +0.8 1,417,306 + -3.6 1,417,485 - -4.1 1,417,620 - MMJJ_RS07725 0.19 -1.5 1,417,667 - MMJJ_RS07725 0.23 -2.3 1,417,680 - MMJJ_RS07725 0.24 -2.7 1,417,680 - MMJJ_RS07725 0.24 -1.7 1,417,682 - MMJJ_RS07725 0.24 -2.1 1,417,683 + MMJJ_RS07725 0.24 -0.9 1,417,704 - -1.3 1,417,714 + -1.3 1,417,716 - MMJJ_RS07725 0.27 -3.5 1,417,716 - MMJJ_RS07725 0.27 -1.7 1,417,718 - MMJJ_RS07725 0.27 -1.3 1,417,719 + MMJJ_RS07725 0.27 -1.9 1,417,833 - MMJJ_RS07725 0.36 -3.6 1,417,853 - MMJJ_RS07725 0.37 -3.9 1,417,908 - MMJJ_RS07725 0.41 -2.9 1,418,101 + MMJJ_RS07725 0.56 -1.7 1,418,124 - -1.5 1,418,162 - MMJJ_RS07725 0.61 -1.9 1,418,372 - MMJJ_RS07725 0.77 -3.4 1,418,645 + -0.6 1,418,651 - -1.6 1,418,676 + -3.8 1,418,737 - -2.1 1,418,752 - -3.1 1,418,753 + -0.9 1,418,753 + -1.7 1,418,756 - -0.5 1,418,760 - -2.3 1,418,760 - -2.0 1,418,761 + -2.4 1,418,766 + -0.2 1,418,766 + -0.9 1,418,774 - +0.9 1,418,774 - -0.1 1,418,775 + +0.3 1,418,775 + -0.5 1,418,775 + +0.9 1,418,776 - -1.2 1,418,776 - -1.5 1,418,777 + -1.3 1,418,777 + +0.6 1,418,777 + +1.2 1,418,778 - +0.5 1,418,778 - +0.2 1,418,778 - -1.9 1,418,778 - -0.7 1,418,778 - +0.9 1,418,778 - -0.4 1,418,778 - +0.3 1,418,779 + -0.6 1,418,780 - +0.1 1,418,780 - +0.9 1,418,781 + +1.0 1,418,784 - +0.6 1,418,784 - -0.3 1,418,784 - +0.6 1,418,785 + +1.0 1,418,785 + -0.4 1,418,787 - -0.6 1,418,787 - -1.7 1,418,787 - +1.2 1,418,823 - -0.8 1,419,044 - MMJJ_RS07735 0.60 -2.3 1,419,080 + +0.6 1,419,080 + +1.5 1,419,129 - -1.0 1,419,129 - +0.3 1,419,129 - -0.3 1,419,130 + -0.9 1,419,130 + +0.2 1,419,130 + +0.6 1,419,130 + +0.5 1,419,130 + +1.0 1,419,131 - +0.0 1,419,131 - +0.8 1,419,131 - +1.2 1,419,131 - +1.0 1,419,132 + +0.7 1,419,132 + +0.2 1,419,132 + +0.4 1,419,132 + +1.1 1,419,132 + +1.6 1,419,132 + -1.0 1,419,132 + +0.9 1,419,133 - -0.4 1,419,133 - -0.3 1,419,133 - +0.1 1,419,133 - -1.5 1,419,148 - -0.9 1,419,149 + +1.1 1,419,158 - -0.7 1,419,159 + +0.2 1,419,162 - +0.2 1,419,163 + -0.0 1,419,165 + -0.1 1,419,175 + +0.1 1,419,184 - +1.8 1,419,184 - +0.5 1,419,185 + -0.5 1,419,185 + +1.7 1,419,228 + +0.6 1,419,319 + MMJJ_RS07740 0.16 +0.2 1,419,328 - MMJJ_RS07740 0.17 -0.7 1,419,359 - MMJJ_RS07740 0.21 +0.1 1,419,359 - MMJJ_RS07740 0.21 +1.1 1,419,360 + MMJJ_RS07740 0.21 -2.9 1,419,364 + MMJJ_RS07740 0.21 -0.1 1,419,370 - MMJJ_RS07740 0.22 +0.3 1,419,371 + MMJJ_RS07740 0.22 -0.3 1,419,425 + MMJJ_RS07740 0.29 -0.1 1,419,484 - MMJJ_RS07740 0.36 +0.7 1,419,485 + MMJJ_RS07740 0.36 -2.7 1,419,534 - MMJJ_RS07740 0.43 -0.1 1,419,534 - MMJJ_RS07740 0.43 +0.3 1,419,613 + MMJJ_RS07740 0.52 -0.2
Or see this region's nucleotide sequence