Experiment: H2 N-free with 2g/L casaminos
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS07400 and MMJJ_RS07405 are separated by 189 nucleotides MMJJ_RS07405 and MMJJ_RS07410 are separated by 129 nucleotides MMJJ_RS07410 and MMJJ_RS07415 are separated by 16 nucleotides MMJJ_RS07415 and MMJJ_RS07420 are separated by 3 nucleotides
MMJJ_RS07400: MMJJ_RS07400 - 2,5-diamino-6-(ribosylamino)-4(3H)-pyrimidinone 5'-phosphate reductase, at 1,359,134 to 1,360,216
_RS07400
MMJJ_RS07405: MMJJ_RS07405 - histone family protein, at 1,360,406 to 1,360,606
_RS07405
MMJJ_RS07410: MMJJ_RS07410 - methanogenesis marker protein Mmp4/MtxX, at 1,360,736 to 1,361,470
_RS07410
MMJJ_RS07415: MMJJ_RS07415 - polyprenyl diphosphate synthase, at 1,361,487 to 1,362,281
_RS07415
MMJJ_RS07420: MMJJ_RS07420 - protease complex subunit PrcB family protein, at 1,362,285 to 1,362,752
_RS07420
Position (kb)
1360
1361
1362 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1359.736 kb on - strand, within MMJJ_RS07400 at 1359.940 kb on + strand, within MMJJ_RS07400 at 1360.055 kb on - strand, within MMJJ_RS07400 at 1360.097 kb on - strand, within MMJJ_RS07400 at 1360.098 kb on + strand, within MMJJ_RS07400 at 1360.098 kb on + strand, within MMJJ_RS07400 at 1360.197 kb on - strand at 1360.197 kb on - strand at 1360.198 kb on + strand at 1360.219 kb on - strand at 1360.219 kb on - strand at 1360.220 kb on + strand at 1360.220 kb on + strand at 1360.288 kb on + strand at 1360.315 kb on - strand at 1360.317 kb on - strand at 1360.332 kb on - strand at 1360.468 kb on + strand, within MMJJ_RS07405 at 1360.471 kb on - strand, within MMJJ_RS07405 at 1360.504 kb on + strand, within MMJJ_RS07405 at 1360.524 kb on - strand, within MMJJ_RS07405 at 1360.546 kb on + strand, within MMJJ_RS07405 at 1360.571 kb on + strand, within MMJJ_RS07405 at 1360.573 kb on - strand, within MMJJ_RS07405 at 1360.574 kb on + strand, within MMJJ_RS07405 at 1360.593 kb on - strand at 1360.604 kb on - strand at 1360.605 kb on + strand at 1360.613 kb on - strand at 1360.638 kb on - strand at 1360.638 kb on - strand at 1360.638 kb on - strand at 1360.638 kb on - strand at 1360.669 kb on - strand at 1360.669 kb on - strand at 1360.671 kb on - strand at 1360.671 kb on - strand at 1360.671 kb on - strand at 1360.671 kb on - strand at 1360.671 kb on - strand at 1360.671 kb on - strand at 1360.671 kb on - strand at 1360.671 kb on - strand at 1360.671 kb on - strand at 1360.672 kb on + strand at 1360.672 kb on + strand at 1360.672 kb on + strand at 1360.672 kb on + strand at 1360.672 kb on + strand at 1360.672 kb on + strand at 1360.672 kb on + strand at 1360.672 kb on + strand at 1360.673 kb on - strand at 1360.673 kb on - strand at 1360.673 kb on - strand at 1360.673 kb on - strand at 1360.673 kb on - strand at 1360.673 kb on - strand at 1360.673 kb on - strand at 1360.674 kb on + strand at 1360.674 kb on + strand at 1360.684 kb on - strand at 1360.685 kb on + strand at 1360.710 kb on + strand at 1360.715 kb on - strand at 1360.716 kb on + strand at 1360.724 kb on + strand at 1360.739 kb on - strand at 1360.739 kb on - strand at 1360.739 kb on - strand at 1361.007 kb on - strand, within MMJJ_RS07410 at 1361.052 kb on - strand at 1361.056 kb on - strand, within MMJJ_RS07410 at 1361.057 kb on + strand at 1361.059 kb on + strand, within MMJJ_RS07410 at 1361.062 kb on - strand, within MMJJ_RS07410 at 1361.082 kb on - strand, within MMJJ_RS07410 at 1361.203 kb on - strand, within MMJJ_RS07410 at 1361.216 kb on + strand, within MMJJ_RS07410 at 1361.396 kb on - strand, within MMJJ_RS07410 at 1361.396 kb on - strand, within MMJJ_RS07410 at 1361.396 kb on - strand, within MMJJ_RS07410 at 1361.397 kb on + strand at 1361.397 kb on + strand at 1361.400 kb on - strand at 1361.401 kb on + strand at 1361.401 kb on + strand at 1361.401 kb on + strand at 1361.401 kb on + strand at 1361.401 kb on + strand at 1361.404 kb on - strand at 1361.404 kb on - strand at 1361.405 kb on + strand at 1361.405 kb on + strand at 1361.602 kb on - strand at 1361.893 kb on + strand, within MMJJ_RS07415 at 1361.893 kb on + strand, within MMJJ_RS07415 at 1361.909 kb on - strand at 1361.913 kb on - strand, within MMJJ_RS07415 at 1361.924 kb on - strand, within MMJJ_RS07415 at 1361.990 kb on + strand, within MMJJ_RS07415 at 1362.052 kb on - strand, within MMJJ_RS07415 at 1362.202 kb on + strand at 1362.300 kb on + strand at 1362.304 kb on - strand at 1362.307 kb on - strand at 1362.339 kb on + strand, within MMJJ_RS07420 at 1362.339 kb on + strand, within MMJJ_RS07420 at 1362.339 kb on + strand, within MMJJ_RS07420 at 1362.339 kb on + strand, within MMJJ_RS07420 at 1362.339 kb on + strand, within MMJJ_RS07420 at 1362.350 kb on - strand, within MMJJ_RS07420 at 1362.350 kb on - strand, within MMJJ_RS07420 at 1362.350 kb on - strand, within MMJJ_RS07420 at 1362.350 kb on - strand, within MMJJ_RS07420 at 1362.351 kb on + strand, within MMJJ_RS07420 at 1362.351 kb on + strand, within MMJJ_RS07420 at 1362.353 kb on - strand, within MMJJ_RS07420 at 1362.383 kb on + strand, within MMJJ_RS07420 at 1362.390 kb on + strand, within MMJJ_RS07420 at 1362.419 kb on + strand, within MMJJ_RS07420 at 1362.425 kb on + strand, within MMJJ_RS07420 at 1362.449 kb on - strand, within MMJJ_RS07420 at 1362.449 kb on - strand, within MMJJ_RS07420 at 1362.449 kb on - strand, within MMJJ_RS07420 at 1362.450 kb on + strand, within MMJJ_RS07420
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 2g/L casaminos remove 1,359,736 - MMJJ_RS07400 0.56 -2.7 1,359,940 + MMJJ_RS07400 0.74 -2.8 1,360,055 - MMJJ_RS07400 0.85 -3.9 1,360,097 - MMJJ_RS07400 0.89 -1.4 1,360,098 + MMJJ_RS07400 0.89 -1.6 1,360,098 + MMJJ_RS07400 0.89 +1.0 1,360,197 - +0.3 1,360,197 - +0.9 1,360,198 + +0.5 1,360,219 - -0.7 1,360,219 - -0.9 1,360,220 + -0.1 1,360,220 + -1.6 1,360,288 + +1.1 1,360,315 - -0.9 1,360,317 - +0.5 1,360,332 - -0.9 1,360,468 + MMJJ_RS07405 0.31 +0.1 1,360,471 - MMJJ_RS07405 0.32 +0.1 1,360,504 + MMJJ_RS07405 0.49 -2.2 1,360,524 - MMJJ_RS07405 0.59 -1.1 1,360,546 + MMJJ_RS07405 0.70 -0.7 1,360,571 + MMJJ_RS07405 0.82 -1.0 1,360,573 - MMJJ_RS07405 0.83 -1.0 1,360,574 + MMJJ_RS07405 0.84 -0.6 1,360,593 - +0.5 1,360,604 - -1.3 1,360,605 + +1.5 1,360,613 - -0.5 1,360,638 - -0.4 1,360,638 - -0.3 1,360,638 - +1.1 1,360,638 - +0.3 1,360,669 - +0.2 1,360,669 - -0.6 1,360,671 - -3.1 1,360,671 - +0.8 1,360,671 - +0.8 1,360,671 - +0.2 1,360,671 - +0.1 1,360,671 - +0.8 1,360,671 - -0.2 1,360,671 - -0.3 1,360,671 - +0.6 1,360,672 + +0.7 1,360,672 + +1.8 1,360,672 + +0.6 1,360,672 + +1.4 1,360,672 + +0.6 1,360,672 + +1.4 1,360,672 + +1.4 1,360,672 + +1.1 1,360,673 - +0.2 1,360,673 - +0.5 1,360,673 - +0.4 1,360,673 - -0.2 1,360,673 - +0.6 1,360,673 - -0.1 1,360,673 - +0.7 1,360,674 + +1.3 1,360,674 + +0.2 1,360,684 - +0.7 1,360,685 + -0.0 1,360,710 + -0.6 1,360,715 - -0.5 1,360,716 + -1.7 1,360,724 + -0.9 1,360,739 - -1.9 1,360,739 - -2.5 1,360,739 - -2.1 1,361,007 - MMJJ_RS07410 0.37 -2.1 1,361,052 - +1.2 1,361,056 - MMJJ_RS07410 0.44 -1.3 1,361,057 + -0.4 1,361,059 + MMJJ_RS07410 0.44 -2.9 1,361,062 - MMJJ_RS07410 0.44 -1.4 1,361,082 - MMJJ_RS07410 0.47 -3.3 1,361,203 - MMJJ_RS07410 0.64 -1.6 1,361,216 + MMJJ_RS07410 0.65 -3.0 1,361,396 - MMJJ_RS07410 0.90 -0.4 1,361,396 - MMJJ_RS07410 0.90 -1.6 1,361,396 - MMJJ_RS07410 0.90 -3.4 1,361,397 + +0.6 1,361,397 + -2.1 1,361,400 - -1.8 1,361,401 + -1.1 1,361,401 + -2.1 1,361,401 + -2.2 1,361,401 + -2.6 1,361,401 + -1.8 1,361,404 - -2.7 1,361,404 - -1.9 1,361,405 + -0.4 1,361,405 + -1.6 1,361,602 - +0.2 1,361,893 + MMJJ_RS07415 0.51 +0.2 1,361,893 + MMJJ_RS07415 0.51 -1.1 1,361,909 - -0.4 1,361,913 - MMJJ_RS07415 0.54 +1.1 1,361,924 - MMJJ_RS07415 0.55 -2.1 1,361,990 + MMJJ_RS07415 0.63 -3.8 1,362,052 - MMJJ_RS07415 0.71 -1.9 1,362,202 + -0.0 1,362,300 + -0.3 1,362,304 - -0.1 1,362,307 - +1.3 1,362,339 + MMJJ_RS07420 0.12 +1.5 1,362,339 + MMJJ_RS07420 0.12 +0.4 1,362,339 + MMJJ_RS07420 0.12 +0.7 1,362,339 + MMJJ_RS07420 0.12 +0.9 1,362,339 + MMJJ_RS07420 0.12 -0.9 1,362,350 - MMJJ_RS07420 0.14 +1.4 1,362,350 - MMJJ_RS07420 0.14 +1.3 1,362,350 - MMJJ_RS07420 0.14 +0.6 1,362,350 - MMJJ_RS07420 0.14 +0.2 1,362,351 + MMJJ_RS07420 0.14 +0.8 1,362,351 + MMJJ_RS07420 0.14 +0.1 1,362,353 - MMJJ_RS07420 0.15 +0.3 1,362,383 + MMJJ_RS07420 0.21 +1.8 1,362,390 + MMJJ_RS07420 0.22 -1.2 1,362,419 + MMJJ_RS07420 0.29 +0.8 1,362,425 + MMJJ_RS07420 0.30 +1.5 1,362,449 - MMJJ_RS07420 0.35 -0.5 1,362,449 - MMJJ_RS07420 0.35 -0.4 1,362,449 - MMJJ_RS07420 0.35 -1.7 1,362,450 + MMJJ_RS07420 0.35 +0.0
Or see this region's nucleotide sequence