Experiment: H2 N-free with 2g/L casaminos
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS07240 and MMJJ_RS07245 are separated by 60 nucleotides MMJJ_RS07245 and MMJJ_RS07250 are separated by 73 nucleotides MMJJ_RS07250 and MMJJ_RS09365 are separated by 20 nucleotides
MMJJ_RS07240: MMJJ_RS07240 - MBL fold metallo-hydrolase, at 1,325,823 to 1,327,091
_RS07240
MMJJ_RS07245: MMJJ_RS07245 - amidohydrolase family protein, at 1,327,152 to 1,328,270
_RS07245
MMJJ_RS07250: MMJJ_RS07250 - thymidylate synthase, at 1,328,344 to 1,328,976
_RS07250
MMJJ_RS09365: MMJJ_RS09365 - hypothetical protein, at 1,328,997 to 1,329,188
_RS09365
Position (kb)
1327
1328
1329 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1326.160 kb on - strand, within MMJJ_RS07240 at 1326.161 kb on + strand, within MMJJ_RS07240 at 1326.169 kb on + strand, within MMJJ_RS07240 at 1326.204 kb on + strand, within MMJJ_RS07240 at 1326.209 kb on - strand, within MMJJ_RS07240 at 1326.209 kb on - strand, within MMJJ_RS07240 at 1326.209 kb on - strand, within MMJJ_RS07240 at 1326.209 kb on - strand, within MMJJ_RS07240 at 1326.210 kb on + strand, within MMJJ_RS07240 at 1326.210 kb on + strand, within MMJJ_RS07240 at 1326.210 kb on + strand, within MMJJ_RS07240 at 1326.247 kb on + strand, within MMJJ_RS07240 at 1326.247 kb on + strand, within MMJJ_RS07240 at 1326.325 kb on - strand, within MMJJ_RS07240 at 1326.325 kb on - strand, within MMJJ_RS07240 at 1326.325 kb on - strand, within MMJJ_RS07240 at 1326.325 kb on - strand, within MMJJ_RS07240 at 1326.326 kb on + strand, within MMJJ_RS07240 at 1326.326 kb on + strand, within MMJJ_RS07240 at 1326.326 kb on + strand, within MMJJ_RS07240 at 1326.326 kb on + strand, within MMJJ_RS07240 at 1326.361 kb on - strand, within MMJJ_RS07240 at 1326.379 kb on + strand, within MMJJ_RS07240 at 1326.397 kb on - strand, within MMJJ_RS07240 at 1326.397 kb on - strand, within MMJJ_RS07240 at 1326.397 kb on - strand, within MMJJ_RS07240 at 1326.397 kb on - strand, within MMJJ_RS07240 at 1326.397 kb on - strand, within MMJJ_RS07240 at 1326.397 kb on - strand, within MMJJ_RS07240 at 1326.398 kb on + strand, within MMJJ_RS07240 at 1326.398 kb on + strand, within MMJJ_RS07240 at 1326.398 kb on + strand, within MMJJ_RS07240 at 1326.398 kb on + strand, within MMJJ_RS07240 at 1326.402 kb on + strand, within MMJJ_RS07240 at 1326.402 kb on + strand, within MMJJ_RS07240 at 1326.453 kb on + strand, within MMJJ_RS07240 at 1326.531 kb on - strand, within MMJJ_RS07240 at 1326.531 kb on - strand, within MMJJ_RS07240 at 1326.531 kb on - strand, within MMJJ_RS07240 at 1326.532 kb on + strand, within MMJJ_RS07240 at 1326.532 kb on + strand, within MMJJ_RS07240 at 1326.532 kb on + strand, within MMJJ_RS07240 at 1326.533 kb on - strand, within MMJJ_RS07240 at 1326.533 kb on - strand, within MMJJ_RS07240 at 1326.534 kb on + strand, within MMJJ_RS07240 at 1326.534 kb on + strand, within MMJJ_RS07240 at 1326.537 kb on - strand, within MMJJ_RS07240 at 1326.537 kb on - strand, within MMJJ_RS07240 at 1326.538 kb on + strand, within MMJJ_RS07240 at 1326.538 kb on + strand, within MMJJ_RS07240 at 1326.538 kb on + strand, within MMJJ_RS07240 at 1326.538 kb on + strand, within MMJJ_RS07240 at 1326.551 kb on + strand, within MMJJ_RS07240 at 1326.567 kb on - strand, within MMJJ_RS07240 at 1326.568 kb on + strand, within MMJJ_RS07240 at 1326.568 kb on + strand, within MMJJ_RS07240 at 1326.632 kb on - strand at 1326.633 kb on + strand, within MMJJ_RS07240 at 1326.633 kb on + strand, within MMJJ_RS07240 at 1326.641 kb on - strand, within MMJJ_RS07240 at 1326.641 kb on - strand, within MMJJ_RS07240 at 1326.642 kb on + strand, within MMJJ_RS07240 at 1326.643 kb on - strand, within MMJJ_RS07240 at 1326.643 kb on - strand, within MMJJ_RS07240 at 1326.643 kb on - strand, within MMJJ_RS07240 at 1326.643 kb on - strand, within MMJJ_RS07240 at 1326.644 kb on + strand, within MMJJ_RS07240 at 1326.644 kb on + strand, within MMJJ_RS07240 at 1326.644 kb on + strand, within MMJJ_RS07240 at 1326.644 kb on + strand, within MMJJ_RS07240 at 1326.644 kb on + strand, within MMJJ_RS07240 at 1326.645 kb on - strand at 1326.646 kb on + strand, within MMJJ_RS07240 at 1326.646 kb on + strand, within MMJJ_RS07240 at 1326.648 kb on - strand, within MMJJ_RS07240 at 1326.670 kb on + strand, within MMJJ_RS07240 at 1326.670 kb on + strand, within MMJJ_RS07240 at 1326.670 kb on + strand, within MMJJ_RS07240 at 1326.673 kb on - strand, within MMJJ_RS07240 at 1326.673 kb on - strand, within MMJJ_RS07240 at 1326.674 kb on + strand, within MMJJ_RS07240 at 1326.691 kb on + strand, within MMJJ_RS07240 at 1326.695 kb on - strand, within MMJJ_RS07240 at 1326.695 kb on - strand, within MMJJ_RS07240 at 1326.696 kb on + strand, within MMJJ_RS07240 at 1326.696 kb on + strand, within MMJJ_RS07240 at 1326.709 kb on - strand, within MMJJ_RS07240 at 1326.709 kb on - strand, within MMJJ_RS07240 at 1326.722 kb on - strand, within MMJJ_RS07240 at 1326.734 kb on - strand, within MMJJ_RS07240 at 1326.753 kb on + strand, within MMJJ_RS07240 at 1326.754 kb on - strand, within MMJJ_RS07240 at 1326.754 kb on - strand, within MMJJ_RS07240 at 1326.755 kb on + strand, within MMJJ_RS07240 at 1326.755 kb on + strand, within MMJJ_RS07240 at 1326.772 kb on + strand, within MMJJ_RS07240 at 1326.772 kb on + strand, within MMJJ_RS07240 at 1326.806 kb on + strand, within MMJJ_RS07240 at 1326.806 kb on + strand, within MMJJ_RS07240 at 1326.827 kb on - strand, within MMJJ_RS07240 at 1326.827 kb on - strand, within MMJJ_RS07240 at 1326.827 kb on - strand, within MMJJ_RS07240 at 1326.837 kb on - strand, within MMJJ_RS07240 at 1326.837 kb on - strand, within MMJJ_RS07240 at 1326.838 kb on + strand, within MMJJ_RS07240 at 1326.844 kb on - strand, within MMJJ_RS07240 at 1326.844 kb on - strand, within MMJJ_RS07240 at 1326.845 kb on + strand, within MMJJ_RS07240 at 1326.845 kb on + strand, within MMJJ_RS07240 at 1326.845 kb on + strand, within MMJJ_RS07240 at 1326.849 kb on - strand, within MMJJ_RS07240 at 1326.850 kb on + strand, within MMJJ_RS07240 at 1326.870 kb on + strand, within MMJJ_RS07240 at 1326.876 kb on - strand, within MMJJ_RS07240 at 1326.896 kb on + strand, within MMJJ_RS07240 at 1326.900 kb on - strand, within MMJJ_RS07240 at 1326.900 kb on - strand, within MMJJ_RS07240 at 1326.901 kb on + strand, within MMJJ_RS07240 at 1326.901 kb on + strand, within MMJJ_RS07240 at 1327.008 kb on - strand at 1327.012 kb on + strand at 1327.033 kb on + strand at 1327.092 kb on - strand at 1327.101 kb on - strand at 1327.102 kb on + strand at 1327.110 kb on - strand at 1327.110 kb on - strand at 1327.112 kb on - strand at 1327.113 kb on + strand at 1327.113 kb on + strand at 1327.141 kb on - strand at 1327.149 kb on + strand at 1327.149 kb on + strand at 1327.153 kb on - strand at 1327.274 kb on + strand, within MMJJ_RS07245 at 1327.367 kb on + strand, within MMJJ_RS07245 at 1327.425 kb on + strand, within MMJJ_RS07245 at 1327.452 kb on + strand, within MMJJ_RS07245 at 1327.516 kb on + strand, within MMJJ_RS07245 at 1327.518 kb on - strand, within MMJJ_RS07245 at 1327.519 kb on + strand, within MMJJ_RS07245 at 1327.519 kb on + strand, within MMJJ_RS07245 at 1327.542 kb on - strand, within MMJJ_RS07245 at 1327.600 kb on + strand, within MMJJ_RS07245 at 1327.908 kb on + strand, within MMJJ_RS07245 at 1327.949 kb on - strand, within MMJJ_RS07245 at 1328.009 kb on - strand, within MMJJ_RS07245 at 1328.013 kb on - strand, within MMJJ_RS07245 at 1328.079 kb on + strand, within MMJJ_RS07245 at 1328.088 kb on + strand, within MMJJ_RS07245 at 1328.121 kb on + strand at 1328.243 kb on + strand at 1328.245 kb on + strand at 1328.289 kb on + strand at 1328.392 kb on + strand at 1328.463 kb on - strand, within MMJJ_RS07250 at 1328.763 kb on + strand, within MMJJ_RS07250 at 1328.975 kb on + strand at 1328.980 kb on - strand at 1329.003 kb on + strand at 1329.012 kb on - strand at 1329.022 kb on + strand, within MMJJ_RS09365 at 1329.040 kb on + strand, within MMJJ_RS09365 at 1329.071 kb on + strand, within MMJJ_RS09365 at 1329.145 kb on + strand, within MMJJ_RS09365 at 1329.176 kb on - strand at 1329.181 kb on + strand at 1329.200 kb on + strand at 1329.209 kb on - strand at 1329.225 kb on + strand at 1329.242 kb on + strand at 1329.244 kb on - strand at 1329.244 kb on - strand at 1329.244 kb on - strand at 1329.245 kb on + strand at 1329.245 kb on + strand at 1329.255 kb on - strand at 1329.255 kb on - strand at 1329.255 kb on - strand at 1329.256 kb on + strand at 1329.260 kb on + strand at 1329.270 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 2g/L casaminos remove 1,326,160 - MMJJ_RS07240 0.27 -0.0 1,326,161 + MMJJ_RS07240 0.27 -1.0 1,326,169 + MMJJ_RS07240 0.27 -0.7 1,326,204 + MMJJ_RS07240 0.30 -3.0 1,326,209 - MMJJ_RS07240 0.30 -0.8 1,326,209 - MMJJ_RS07240 0.30 -0.8 1,326,209 - MMJJ_RS07240 0.30 -0.4 1,326,209 - MMJJ_RS07240 0.30 +0.7 1,326,210 + MMJJ_RS07240 0.30 +0.3 1,326,210 + MMJJ_RS07240 0.30 +1.4 1,326,210 + MMJJ_RS07240 0.30 +0.1 1,326,247 + MMJJ_RS07240 0.33 -0.6 1,326,247 + MMJJ_RS07240 0.33 -0.1 1,326,325 - MMJJ_RS07240 0.40 +0.7 1,326,325 - MMJJ_RS07240 0.40 -3.3 1,326,325 - MMJJ_RS07240 0.40 -0.7 1,326,325 - MMJJ_RS07240 0.40 -0.3 1,326,326 + MMJJ_RS07240 0.40 +0.9 1,326,326 + MMJJ_RS07240 0.40 +1.7 1,326,326 + MMJJ_RS07240 0.40 +0.7 1,326,326 + MMJJ_RS07240 0.40 +0.6 1,326,361 - MMJJ_RS07240 0.42 +1.3 1,326,379 + MMJJ_RS07240 0.44 +1.1 1,326,397 - MMJJ_RS07240 0.45 +0.3 1,326,397 - MMJJ_RS07240 0.45 -2.2 1,326,397 - MMJJ_RS07240 0.45 -0.4 1,326,397 - MMJJ_RS07240 0.45 +1.4 1,326,397 - MMJJ_RS07240 0.45 -1.3 1,326,397 - MMJJ_RS07240 0.45 -0.4 1,326,398 + MMJJ_RS07240 0.45 -0.7 1,326,398 + MMJJ_RS07240 0.45 +0.3 1,326,398 + MMJJ_RS07240 0.45 +0.2 1,326,398 + MMJJ_RS07240 0.45 +0.7 1,326,402 + MMJJ_RS07240 0.46 +0.6 1,326,402 + MMJJ_RS07240 0.46 +0.4 1,326,453 + MMJJ_RS07240 0.50 +1.2 1,326,531 - MMJJ_RS07240 0.56 -1.2 1,326,531 - MMJJ_RS07240 0.56 -1.7 1,326,531 - MMJJ_RS07240 0.56 -0.6 1,326,532 + MMJJ_RS07240 0.56 -0.5 1,326,532 + MMJJ_RS07240 0.56 +0.8 1,326,532 + MMJJ_RS07240 0.56 +0.8 1,326,533 - MMJJ_RS07240 0.56 -1.0 1,326,533 - MMJJ_RS07240 0.56 +0.1 1,326,534 + MMJJ_RS07240 0.56 -1.5 1,326,534 + MMJJ_RS07240 0.56 +0.4 1,326,537 - MMJJ_RS07240 0.56 +1.1 1,326,537 - MMJJ_RS07240 0.56 -0.2 1,326,538 + MMJJ_RS07240 0.56 -0.8 1,326,538 + MMJJ_RS07240 0.56 -1.2 1,326,538 + MMJJ_RS07240 0.56 -1.1 1,326,538 + MMJJ_RS07240 0.56 -1.5 1,326,551 + MMJJ_RS07240 0.57 +0.8 1,326,567 - MMJJ_RS07240 0.59 +1.9 1,326,568 + MMJJ_RS07240 0.59 +0.2 1,326,568 + MMJJ_RS07240 0.59 -1.1 1,326,632 - -0.4 1,326,633 + MMJJ_RS07240 0.64 -0.7 1,326,633 + MMJJ_RS07240 0.64 +1.0 1,326,641 - MMJJ_RS07240 0.64 +0.5 1,326,641 - MMJJ_RS07240 0.64 +0.8 1,326,642 + MMJJ_RS07240 0.65 +0.3 1,326,643 - MMJJ_RS07240 0.65 +1.1 1,326,643 - MMJJ_RS07240 0.65 -0.2 1,326,643 - MMJJ_RS07240 0.65 -1.4 1,326,643 - MMJJ_RS07240 0.65 -0.3 1,326,644 + MMJJ_RS07240 0.65 +1.3 1,326,644 + MMJJ_RS07240 0.65 +0.8 1,326,644 + MMJJ_RS07240 0.65 -0.6 1,326,644 + MMJJ_RS07240 0.65 +1.5 1,326,644 + MMJJ_RS07240 0.65 -0.7 1,326,645 - -1.4 1,326,646 + MMJJ_RS07240 0.65 -1.9 1,326,646 + MMJJ_RS07240 0.65 +1.8 1,326,648 - MMJJ_RS07240 0.65 -0.0 1,326,670 + MMJJ_RS07240 0.67 +1.3 1,326,670 + MMJJ_RS07240 0.67 +0.0 1,326,670 + MMJJ_RS07240 0.67 +0.3 1,326,673 - MMJJ_RS07240 0.67 +0.6 1,326,673 - MMJJ_RS07240 0.67 +1.0 1,326,674 + MMJJ_RS07240 0.67 -1.1 1,326,691 + MMJJ_RS07240 0.68 +0.3 1,326,695 - MMJJ_RS07240 0.69 +0.7 1,326,695 - MMJJ_RS07240 0.69 -3.1 1,326,696 + MMJJ_RS07240 0.69 +1.4 1,326,696 + MMJJ_RS07240 0.69 +0.9 1,326,709 - MMJJ_RS07240 0.70 +0.7 1,326,709 - MMJJ_RS07240 0.70 +0.2 1,326,722 - MMJJ_RS07240 0.71 +1.6 1,326,734 - MMJJ_RS07240 0.72 -1.0 1,326,753 + MMJJ_RS07240 0.73 +1.2 1,326,754 - MMJJ_RS07240 0.73 +0.4 1,326,754 - MMJJ_RS07240 0.73 +0.1 1,326,755 + MMJJ_RS07240 0.73 -0.7 1,326,755 + MMJJ_RS07240 0.73 -1.0 1,326,772 + MMJJ_RS07240 0.75 +0.2 1,326,772 + MMJJ_RS07240 0.75 +0.6 1,326,806 + MMJJ_RS07240 0.77 +0.3 1,326,806 + MMJJ_RS07240 0.77 +0.8 1,326,827 - MMJJ_RS07240 0.79 +0.5 1,326,827 - MMJJ_RS07240 0.79 -1.3 1,326,827 - MMJJ_RS07240 0.79 -0.4 1,326,837 - MMJJ_RS07240 0.80 +1.4 1,326,837 - MMJJ_RS07240 0.80 -4.6 1,326,838 + MMJJ_RS07240 0.80 -0.1 1,326,844 - MMJJ_RS07240 0.80 +1.7 1,326,844 - MMJJ_RS07240 0.80 -0.5 1,326,845 + MMJJ_RS07240 0.81 +1.0 1,326,845 + MMJJ_RS07240 0.81 -1.1 1,326,845 + MMJJ_RS07240 0.81 -1.0 1,326,849 - MMJJ_RS07240 0.81 +1.1 1,326,850 + MMJJ_RS07240 0.81 -0.3 1,326,870 + MMJJ_RS07240 0.83 -0.4 1,326,876 - MMJJ_RS07240 0.83 +1.1 1,326,896 + MMJJ_RS07240 0.85 +0.9 1,326,900 - MMJJ_RS07240 0.85 -0.6 1,326,900 - MMJJ_RS07240 0.85 -0.1 1,326,901 + MMJJ_RS07240 0.85 -0.8 1,326,901 + MMJJ_RS07240 0.85 +0.3 1,327,008 - +2.4 1,327,012 + -0.3 1,327,033 + -1.7 1,327,092 - +0.9 1,327,101 - +1.0 1,327,102 + +0.9 1,327,110 - +0.9 1,327,110 - -0.7 1,327,112 - -1.0 1,327,113 + +0.7 1,327,113 + -0.2 1,327,141 - +0.5 1,327,149 + -0.8 1,327,149 + -0.3 1,327,153 - -0.7 1,327,274 + MMJJ_RS07245 0.11 -0.5 1,327,367 + MMJJ_RS07245 0.19 -0.4 1,327,425 + MMJJ_RS07245 0.24 -1.7 1,327,452 + MMJJ_RS07245 0.27 -1.8 1,327,516 + MMJJ_RS07245 0.33 -2.4 1,327,518 - MMJJ_RS07245 0.33 -1.2 1,327,519 + MMJJ_RS07245 0.33 -2.6 1,327,519 + MMJJ_RS07245 0.33 -1.6 1,327,542 - MMJJ_RS07245 0.35 -3.2 1,327,600 + MMJJ_RS07245 0.40 -1.6 1,327,908 + MMJJ_RS07245 0.68 -3.5 1,327,949 - MMJJ_RS07245 0.71 -2.4 1,328,009 - MMJJ_RS07245 0.77 -1.4 1,328,013 - MMJJ_RS07245 0.77 -2.9 1,328,079 + MMJJ_RS07245 0.83 -0.4 1,328,088 + MMJJ_RS07245 0.84 -2.7 1,328,121 + -0.4 1,328,243 + -1.8 1,328,245 + -1.7 1,328,289 + +0.7 1,328,392 + +1.2 1,328,463 - MMJJ_RS07250 0.19 -2.0 1,328,763 + MMJJ_RS07250 0.66 -1.3 1,328,975 + +0.2 1,328,980 - -0.1 1,329,003 + -0.8 1,329,012 - -0.7 1,329,022 + MMJJ_RS09365 0.13 +1.3 1,329,040 + MMJJ_RS09365 0.22 -0.4 1,329,071 + MMJJ_RS09365 0.39 +1.5 1,329,145 + MMJJ_RS09365 0.77 +1.1 1,329,176 - +0.8 1,329,181 + -0.8 1,329,200 + +0.4 1,329,209 - +0.5 1,329,225 + +0.1 1,329,242 + -0.0 1,329,244 - +1.0 1,329,244 - -1.2 1,329,244 - -1.4 1,329,245 + -1.1 1,329,245 + +1.1 1,329,255 - +0.4 1,329,255 - +0.1 1,329,255 - +0.4 1,329,256 + +0.4 1,329,260 + +1.1 1,329,270 - +1.5
Or see this region's nucleotide sequence