Experiment: H2 N-free with 2g/L casaminos
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS06835 and MMJJ_RS06840 are separated by 28 nucleotides MMJJ_RS06840 and MMJJ_RS06845 are separated by 13 nucleotides MMJJ_RS06845 and MMJJ_RS06850 are separated by 22 nucleotides MMJJ_RS06850 and MMJJ_RS06855 are separated by 15 nucleotides
MMJJ_RS06835: MMJJ_RS06835 - 4Fe-4S binding protein, at 1,261,378 to 1,262,559
_RS06835
MMJJ_RS06840: MMJJ_RS06840 - nickel-dependent hydrogenase large subunit, at 1,262,588 to 1,263,715
_RS06840
MMJJ_RS06845: MMJJ_RS06845 - NADH-quinone oxidoreductase subunit B family protein, at 1,263,729 to 1,264,199
_RS06845
MMJJ_RS06850: MMJJ_RS06850 - DUF1959 domain-containing protein, at 1,264,222 to 1,264,632
_RS06850
MMJJ_RS06855: MMJJ_RS06855 - DUF2104 domain-containing protein, at 1,264,648 to 1,264,947
_RS06855
Position (kb)
1262
1263
1264 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1261.601 kb on + strand, within MMJJ_RS06835 at 1261.601 kb on + strand, within MMJJ_RS06835 at 1261.612 kb on - strand, within MMJJ_RS06835 at 1261.613 kb on + strand, within MMJJ_RS06835 at 1261.613 kb on + strand, within MMJJ_RS06835 at 1261.620 kb on + strand, within MMJJ_RS06835 at 1261.635 kb on + strand, within MMJJ_RS06835 at 1261.661 kb on + strand, within MMJJ_RS06835 at 1261.680 kb on - strand, within MMJJ_RS06835 at 1261.681 kb on + strand, within MMJJ_RS06835 at 1261.692 kb on + strand, within MMJJ_RS06835 at 1261.692 kb on + strand, within MMJJ_RS06835 at 1261.734 kb on + strand, within MMJJ_RS06835 at 1261.822 kb on - strand, within MMJJ_RS06835 at 1261.822 kb on - strand, within MMJJ_RS06835 at 1261.823 kb on + strand, within MMJJ_RS06835 at 1261.840 kb on + strand, within MMJJ_RS06835 at 1261.840 kb on + strand, within MMJJ_RS06835 at 1261.855 kb on - strand, within MMJJ_RS06835 at 1261.855 kb on - strand, within MMJJ_RS06835 at 1261.897 kb on + strand, within MMJJ_RS06835 at 1261.897 kb on + strand, within MMJJ_RS06835 at 1261.897 kb on + strand, within MMJJ_RS06835 at 1261.900 kb on - strand, within MMJJ_RS06835 at 1261.901 kb on + strand, within MMJJ_RS06835 at 1261.901 kb on + strand, within MMJJ_RS06835 at 1261.934 kb on + strand, within MMJJ_RS06835 at 1261.984 kb on + strand, within MMJJ_RS06835 at 1261.986 kb on - strand, within MMJJ_RS06835 at 1262.030 kb on - strand, within MMJJ_RS06835 at 1262.031 kb on + strand, within MMJJ_RS06835 at 1262.035 kb on + strand, within MMJJ_RS06835 at 1262.060 kb on + strand, within MMJJ_RS06835 at 1262.060 kb on + strand, within MMJJ_RS06835 at 1262.060 kb on + strand, within MMJJ_RS06835 at 1262.083 kb on - strand, within MMJJ_RS06835 at 1262.101 kb on - strand, within MMJJ_RS06835 at 1262.101 kb on - strand, within MMJJ_RS06835 at 1262.102 kb on + strand, within MMJJ_RS06835 at 1262.108 kb on - strand, within MMJJ_RS06835 at 1262.109 kb on + strand, within MMJJ_RS06835 at 1262.174 kb on + strand, within MMJJ_RS06835 at 1262.195 kb on + strand, within MMJJ_RS06835 at 1262.195 kb on + strand, within MMJJ_RS06835 at 1262.195 kb on + strand, within MMJJ_RS06835 at 1262.197 kb on - strand, within MMJJ_RS06835 at 1262.197 kb on - strand, within MMJJ_RS06835 at 1262.198 kb on + strand, within MMJJ_RS06835 at 1262.198 kb on + strand, within MMJJ_RS06835 at 1262.306 kb on + strand, within MMJJ_RS06835 at 1262.394 kb on - strand, within MMJJ_RS06835 at 1262.410 kb on - strand, within MMJJ_RS06835 at 1262.411 kb on + strand, within MMJJ_RS06835 at 1262.411 kb on + strand, within MMJJ_RS06835 at 1262.413 kb on + strand, within MMJJ_RS06835 at 1262.469 kb on - strand at 1262.500 kb on + strand at 1262.509 kb on + strand at 1262.541 kb on + strand at 1262.559 kb on - strand at 1262.559 kb on - strand at 1262.559 kb on - strand at 1262.559 kb on - strand at 1262.559 kb on - strand at 1262.559 kb on - strand at 1262.559 kb on - strand at 1262.559 kb on - strand at 1262.560 kb on + strand at 1262.560 kb on + strand at 1262.560 kb on + strand at 1262.560 kb on + strand at 1262.560 kb on + strand at 1262.560 kb on + strand at 1262.560 kb on + strand at 1262.563 kb on - strand at 1262.563 kb on - strand at 1262.564 kb on + strand at 1262.564 kb on + strand at 1262.564 kb on + strand at 1262.564 kb on + strand at 1262.564 kb on + strand at 1262.582 kb on - strand at 1262.586 kb on - strand at 1262.617 kb on - strand at 1262.617 kb on - strand at 1262.621 kb on - strand at 1262.621 kb on - strand at 1262.630 kb on - strand at 1262.630 kb on - strand at 1262.638 kb on - strand at 1262.639 kb on + strand at 1262.650 kb on - strand at 1262.650 kb on - strand at 1262.680 kb on + strand at 1262.680 kb on + strand at 1262.680 kb on + strand at 1262.724 kb on - strand, within MMJJ_RS06840 at 1262.725 kb on + strand, within MMJJ_RS06840 at 1262.777 kb on - strand, within MMJJ_RS06840 at 1263.127 kb on - strand, within MMJJ_RS06840 at 1263.271 kb on - strand, within MMJJ_RS06840 at 1263.285 kb on + strand, within MMJJ_RS06840 at 1263.299 kb on + strand, within MMJJ_RS06840 at 1263.430 kb on - strand, within MMJJ_RS06840 at 1263.435 kb on + strand, within MMJJ_RS06840 at 1263.516 kb on - strand, within MMJJ_RS06840 at 1263.516 kb on - strand, within MMJJ_RS06840 at 1263.553 kb on - strand at 1263.565 kb on + strand, within MMJJ_RS06840 at 1263.633 kb on + strand at 1263.715 kb on - strand at 1263.718 kb on - strand at 1263.902 kb on - strand, within MMJJ_RS06845 at 1263.915 kb on + strand, within MMJJ_RS06845 at 1264.061 kb on - strand at 1264.117 kb on + strand, within MMJJ_RS06845 at 1264.223 kb on - strand at 1264.224 kb on + strand at 1264.246 kb on + strand at 1264.252 kb on + strand at 1264.252 kb on + strand at 1264.381 kb on - strand, within MMJJ_RS06850 at 1264.627 kb on - strand at 1264.649 kb on - strand at 1264.650 kb on + strand at 1264.666 kb on - strand at 1264.670 kb on + strand at 1264.683 kb on - strand, within MMJJ_RS06855 at 1264.684 kb on + strand at 1264.693 kb on - strand, within MMJJ_RS06855
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 2g/L casaminos remove 1,261,601 + MMJJ_RS06835 0.19 -2.6 1,261,601 + MMJJ_RS06835 0.19 -3.8 1,261,612 - MMJJ_RS06835 0.20 -2.5 1,261,613 + MMJJ_RS06835 0.20 -0.8 1,261,613 + MMJJ_RS06835 0.20 -0.8 1,261,620 + MMJJ_RS06835 0.20 -1.8 1,261,635 + MMJJ_RS06835 0.22 -1.4 1,261,661 + MMJJ_RS06835 0.24 -1.5 1,261,680 - MMJJ_RS06835 0.26 +0.5 1,261,681 + MMJJ_RS06835 0.26 -2.8 1,261,692 + MMJJ_RS06835 0.27 -2.2 1,261,692 + MMJJ_RS06835 0.27 -3.3 1,261,734 + MMJJ_RS06835 0.30 -1.4 1,261,822 - MMJJ_RS06835 0.38 -2.8 1,261,822 - MMJJ_RS06835 0.38 -2.8 1,261,823 + MMJJ_RS06835 0.38 -1.4 1,261,840 + MMJJ_RS06835 0.39 -0.6 1,261,840 + MMJJ_RS06835 0.39 -2.2 1,261,855 - MMJJ_RS06835 0.40 -1.4 1,261,855 - MMJJ_RS06835 0.40 -1.4 1,261,897 + MMJJ_RS06835 0.44 -0.5 1,261,897 + MMJJ_RS06835 0.44 -1.1 1,261,897 + MMJJ_RS06835 0.44 -3.0 1,261,900 - MMJJ_RS06835 0.44 -3.5 1,261,901 + MMJJ_RS06835 0.44 -0.2 1,261,901 + MMJJ_RS06835 0.44 -2.0 1,261,934 + MMJJ_RS06835 0.47 -1.5 1,261,984 + MMJJ_RS06835 0.51 -1.1 1,261,986 - MMJJ_RS06835 0.51 -1.3 1,262,030 - MMJJ_RS06835 0.55 -0.0 1,262,031 + MMJJ_RS06835 0.55 -3.4 1,262,035 + MMJJ_RS06835 0.56 -1.3 1,262,060 + MMJJ_RS06835 0.58 -2.9 1,262,060 + MMJJ_RS06835 0.58 -1.7 1,262,060 + MMJJ_RS06835 0.58 -0.3 1,262,083 - MMJJ_RS06835 0.60 -2.0 1,262,101 - MMJJ_RS06835 0.61 -1.6 1,262,101 - MMJJ_RS06835 0.61 -1.4 1,262,102 + MMJJ_RS06835 0.61 -1.1 1,262,108 - MMJJ_RS06835 0.62 -0.2 1,262,109 + MMJJ_RS06835 0.62 -2.8 1,262,174 + MMJJ_RS06835 0.67 -1.5 1,262,195 + MMJJ_RS06835 0.69 -0.8 1,262,195 + MMJJ_RS06835 0.69 -2.5 1,262,195 + MMJJ_RS06835 0.69 -1.7 1,262,197 - MMJJ_RS06835 0.69 -0.1 1,262,197 - MMJJ_RS06835 0.69 -1.2 1,262,198 + MMJJ_RS06835 0.69 -1.7 1,262,198 + MMJJ_RS06835 0.69 -1.2 1,262,306 + MMJJ_RS06835 0.79 -2.5 1,262,394 - MMJJ_RS06835 0.86 -1.6 1,262,410 - MMJJ_RS06835 0.87 -0.3 1,262,411 + MMJJ_RS06835 0.87 -1.5 1,262,411 + MMJJ_RS06835 0.87 -3.7 1,262,413 + MMJJ_RS06835 0.88 -1.2 1,262,469 - -0.7 1,262,500 + -2.0 1,262,509 + -3.2 1,262,541 + -1.7 1,262,559 - -0.2 1,262,559 - -2.7 1,262,559 - -2.2 1,262,559 - -3.6 1,262,559 - -0.7 1,262,559 - -2.6 1,262,559 - -3.4 1,262,559 - +0.3 1,262,560 + -1.3 1,262,560 + -0.9 1,262,560 + -2.1 1,262,560 + -1.8 1,262,560 + -1.5 1,262,560 + -2.1 1,262,560 + -1.5 1,262,563 - -2.0 1,262,563 - -0.7 1,262,564 + -0.4 1,262,564 + -0.7 1,262,564 + -2.5 1,262,564 + -1.8 1,262,564 + -1.9 1,262,582 - -0.6 1,262,586 - -1.3 1,262,617 - +0.8 1,262,617 - -0.8 1,262,621 - -1.3 1,262,621 - -0.6 1,262,630 - -0.7 1,262,630 - -0.8 1,262,638 - -1.3 1,262,639 + -2.5 1,262,650 - -2.7 1,262,650 - -2.3 1,262,680 + -0.7 1,262,680 + -1.2 1,262,680 + -1.3 1,262,724 - MMJJ_RS06840 0.12 -2.4 1,262,725 + MMJJ_RS06840 0.12 -2.9 1,262,777 - MMJJ_RS06840 0.17 -2.6 1,263,127 - MMJJ_RS06840 0.48 -1.3 1,263,271 - MMJJ_RS06840 0.61 -1.3 1,263,285 + MMJJ_RS06840 0.62 -2.2 1,263,299 + MMJJ_RS06840 0.63 -1.7 1,263,430 - MMJJ_RS06840 0.75 -1.9 1,263,435 + MMJJ_RS06840 0.75 -0.9 1,263,516 - MMJJ_RS06840 0.82 -3.1 1,263,516 - MMJJ_RS06840 0.82 -0.1 1,263,553 - -0.7 1,263,565 + MMJJ_RS06840 0.87 -1.3 1,263,633 + +1.0 1,263,715 - -0.9 1,263,718 - -0.3 1,263,902 - MMJJ_RS06845 0.37 -1.0 1,263,915 + MMJJ_RS06845 0.39 -0.0 1,264,061 - -0.3 1,264,117 + MMJJ_RS06845 0.82 -1.7 1,264,223 - +1.0 1,264,224 + -0.7 1,264,246 + -3.5 1,264,252 + -1.9 1,264,252 + -1.5 1,264,381 - MMJJ_RS06850 0.39 -2.0 1,264,627 - -1.2 1,264,649 - -0.0 1,264,650 + -0.7 1,264,666 - -1.1 1,264,670 + -0.7 1,264,683 - MMJJ_RS06855 0.12 -2.5 1,264,684 + -0.3 1,264,693 - MMJJ_RS06855 0.15 -1.7
Or see this region's nucleotide sequence