Experiment: H2 N-free with 2g/L casaminos
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS06715 and MMJJ_RS06720 are separated by 124 nucleotides MMJJ_RS06720 and MMJJ_RS06725 are separated by 22 nucleotides MMJJ_RS06725 and MMJJ_RS06730 are separated by 2 nucleotides MMJJ_RS06730 and MMJJ_RS06735 are separated by 64 nucleotides
MMJJ_RS06715: MMJJ_RS06715 - lactaldehyde dehydrogenase, at 1,240,060 to 1,241,457
_RS06715
MMJJ_RS06720: MMJJ_RS06720 - mechanosensitive ion channel family protein, at 1,241,582 to 1,242,385
_RS06720
MMJJ_RS06725: MMJJ_RS06725 - MogA/MoaB family molybdenum cofactor biosynthesis protein, at 1,242,408 to 1,242,893
_RS06725
MMJJ_RS06730: MMJJ_RS06730 - ATP-binding cassette domain-containing protein, at 1,242,896 to 1,243,732
_RS06730
MMJJ_RS06735: MMJJ_RS06735 - cobalt ECF transporter T component CbiQ, at 1,243,797 to 1,244,570
_RS06735
Position (kb)
1242
1243 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 1241.432 kb on + strand at 1241.433 kb on - strand at 1241.434 kb on + strand at 1241.485 kb on - strand at 1241.488 kb on - strand at 1241.489 kb on + strand at 1241.489 kb on + strand at 1241.491 kb on - strand at 1241.503 kb on - strand at 1241.503 kb on - strand at 1241.504 kb on + strand at 1241.504 kb on + strand at 1241.506 kb on - strand at 1241.526 kb on - strand at 1241.526 kb on - strand at 1241.527 kb on + strand at 1241.527 kb on + strand at 1241.527 kb on + strand at 1241.527 kb on + strand at 1241.529 kb on - strand at 1241.529 kb on - strand at 1241.531 kb on - strand at 1241.531 kb on - strand at 1241.532 kb on + strand at 1241.532 kb on + strand at 1241.556 kb on - strand at 1241.614 kb on - strand at 1241.617 kb on + strand at 1241.617 kb on + strand at 1241.628 kb on - strand at 1241.634 kb on - strand at 1241.635 kb on + strand at 1241.635 kb on + strand at 1241.658 kb on - strand at 1241.664 kb on + strand, within MMJJ_RS06720 at 1241.687 kb on + strand, within MMJJ_RS06720 at 1241.690 kb on + strand at 1241.734 kb on + strand, within MMJJ_RS06720 at 1241.734 kb on + strand, within MMJJ_RS06720 at 1241.754 kb on - strand, within MMJJ_RS06720 at 1241.754 kb on - strand, within MMJJ_RS06720 at 1241.755 kb on + strand, within MMJJ_RS06720 at 1241.755 kb on + strand, within MMJJ_RS06720 at 1241.771 kb on - strand, within MMJJ_RS06720 at 1241.771 kb on - strand, within MMJJ_RS06720 at 1241.771 kb on - strand, within MMJJ_RS06720 at 1241.772 kb on + strand, within MMJJ_RS06720 at 1241.776 kb on + strand, within MMJJ_RS06720 at 1241.776 kb on + strand, within MMJJ_RS06720 at 1241.781 kb on - strand, within MMJJ_RS06720 at 1241.782 kb on + strand, within MMJJ_RS06720 at 1241.785 kb on + strand, within MMJJ_RS06720 at 1241.827 kb on + strand, within MMJJ_RS06720 at 1241.888 kb on + strand, within MMJJ_RS06720 at 1241.888 kb on + strand, within MMJJ_RS06720 at 1241.921 kb on + strand, within MMJJ_RS06720 at 1241.955 kb on - strand, within MMJJ_RS06720 at 1241.955 kb on - strand, within MMJJ_RS06720 at 1241.956 kb on + strand, within MMJJ_RS06720 at 1242.020 kb on + strand, within MMJJ_RS06720 at 1242.048 kb on - strand, within MMJJ_RS06720 at 1242.048 kb on - strand, within MMJJ_RS06720 at 1242.049 kb on + strand, within MMJJ_RS06720 at 1242.064 kb on - strand, within MMJJ_RS06720 at 1242.090 kb on - strand, within MMJJ_RS06720 at 1242.122 kb on - strand, within MMJJ_RS06720 at 1242.167 kb on + strand, within MMJJ_RS06720 at 1242.187 kb on + strand, within MMJJ_RS06720 at 1242.211 kb on + strand, within MMJJ_RS06720 at 1242.216 kb on - strand, within MMJJ_RS06720 at 1242.278 kb on - strand, within MMJJ_RS06720 at 1242.302 kb on + strand, within MMJJ_RS06720 at 1242.312 kb on - strand at 1242.366 kb on - strand at 1242.366 kb on - strand at 1242.366 kb on - strand at 1242.367 kb on + strand at 1242.367 kb on + strand at 1242.367 kb on + strand at 1242.367 kb on + strand at 1242.367 kb on + strand at 1242.367 kb on + strand at 1242.370 kb on - strand at 1242.380 kb on - strand at 1242.398 kb on + strand at 1242.414 kb on - strand at 1242.415 kb on + strand at 1242.446 kb on - strand at 1242.446 kb on - strand at 1242.447 kb on + strand at 1242.447 kb on + strand at 1242.467 kb on - strand, within MMJJ_RS06725 at 1242.468 kb on + strand, within MMJJ_RS06725 at 1242.480 kb on + strand, within MMJJ_RS06725 at 1242.480 kb on + strand, within MMJJ_RS06725 at 1242.497 kb on + strand, within MMJJ_RS06725 at 1242.500 kb on - strand, within MMJJ_RS06725 at 1242.513 kb on + strand, within MMJJ_RS06725 at 1242.514 kb on - strand, within MMJJ_RS06725 at 1242.515 kb on + strand, within MMJJ_RS06725 at 1242.550 kb on - strand, within MMJJ_RS06725 at 1242.550 kb on - strand, within MMJJ_RS06725 at 1242.551 kb on + strand, within MMJJ_RS06725 at 1242.551 kb on + strand, within MMJJ_RS06725 at 1242.565 kb on - strand, within MMJJ_RS06725 at 1242.566 kb on + strand, within MMJJ_RS06725 at 1242.570 kb on - strand, within MMJJ_RS06725 at 1242.575 kb on - strand, within MMJJ_RS06725 at 1242.575 kb on - strand, within MMJJ_RS06725 at 1242.585 kb on - strand, within MMJJ_RS06725 at 1242.586 kb on + strand, within MMJJ_RS06725 at 1242.639 kb on + strand, within MMJJ_RS06725 at 1242.641 kb on - strand, within MMJJ_RS06725 at 1242.641 kb on - strand, within MMJJ_RS06725 at 1242.641 kb on - strand, within MMJJ_RS06725 at 1242.642 kb on + strand, within MMJJ_RS06725 at 1242.650 kb on - strand, within MMJJ_RS06725 at 1242.650 kb on - strand, within MMJJ_RS06725 at 1242.675 kb on - strand, within MMJJ_RS06725 at 1242.681 kb on + strand, within MMJJ_RS06725 at 1242.681 kb on + strand, within MMJJ_RS06725 at 1242.681 kb on + strand, within MMJJ_RS06725 at 1242.681 kb on + strand, within MMJJ_RS06725 at 1242.681 kb on + strand, within MMJJ_RS06725 at 1242.696 kb on + strand, within MMJJ_RS06725 at 1242.707 kb on - strand, within MMJJ_RS06725 at 1242.707 kb on - strand, within MMJJ_RS06725 at 1242.707 kb on - strand, within MMJJ_RS06725 at 1242.708 kb on + strand at 1242.708 kb on + strand, within MMJJ_RS06725 at 1242.708 kb on + strand, within MMJJ_RS06725 at 1242.708 kb on + strand, within MMJJ_RS06725 at 1242.708 kb on + strand, within MMJJ_RS06725 at 1242.708 kb on + strand, within MMJJ_RS06725 at 1242.708 kb on + strand, within MMJJ_RS06725 at 1242.717 kb on - strand, within MMJJ_RS06725 at 1242.718 kb on + strand, within MMJJ_RS06725 at 1242.738 kb on + strand, within MMJJ_RS06725 at 1242.750 kb on - strand, within MMJJ_RS06725 at 1242.750 kb on - strand, within MMJJ_RS06725 at 1242.751 kb on + strand, within MMJJ_RS06725 at 1242.771 kb on - strand, within MMJJ_RS06725 at 1242.808 kb on + strand, within MMJJ_RS06725 at 1242.808 kb on + strand, within MMJJ_RS06725 at 1242.850 kb on + strand at 1242.850 kb on + strand at 1242.850 kb on + strand at 1242.850 kb on + strand at 1242.850 kb on + strand at 1242.854 kb on - strand at 1242.854 kb on - strand at 1242.855 kb on + strand at 1242.855 kb on + strand at 1242.870 kb on - strand at 1242.898 kb on + strand at 1243.020 kb on + strand, within MMJJ_RS06730 at 1243.033 kb on - strand, within MMJJ_RS06730 at 1243.034 kb on + strand, within MMJJ_RS06730 at 1243.097 kb on - strand, within MMJJ_RS06730 at 1243.097 kb on - strand, within MMJJ_RS06730 at 1243.105 kb on + strand, within MMJJ_RS06730 at 1243.119 kb on - strand, within MMJJ_RS06730 at 1243.120 kb on + strand, within MMJJ_RS06730 at 1243.120 kb on + strand, within MMJJ_RS06730 at 1243.122 kb on + strand, within MMJJ_RS06730 at 1243.130 kb on - strand, within MMJJ_RS06730 at 1243.130 kb on - strand, within MMJJ_RS06730 at 1243.136 kb on - strand, within MMJJ_RS06730 at 1243.137 kb on + strand, within MMJJ_RS06730 at 1243.158 kb on + strand, within MMJJ_RS06730 at 1243.178 kb on - strand, within MMJJ_RS06730 at 1243.178 kb on - strand, within MMJJ_RS06730 at 1243.314 kb on + strand, within MMJJ_RS06730 at 1243.551 kb on + strand, within MMJJ_RS06730 at 1243.701 kb on - strand at 1243.701 kb on - strand at 1243.702 kb on + strand at 1243.706 kb on - strand at 1243.752 kb on - strand at 1243.752 kb on - strand at 1243.752 kb on - strand at 1243.752 kb on - strand at 1243.752 kb on - strand at 1243.753 kb on + strand at 1243.753 kb on + strand at 1243.753 kb on + strand at 1243.753 kb on + strand at 1243.753 kb on + strand at 1243.753 kb on + strand at 1243.753 kb on + strand at 1243.753 kb on + strand at 1243.753 kb on + strand at 1243.753 kb on + strand at 1243.753 kb on + strand at 1243.754 kb on - strand at 1243.754 kb on - strand at 1243.754 kb on - strand at 1243.761 kb on + strand at 1243.766 kb on - strand at 1243.767 kb on + strand at 1243.785 kb on - strand at 1243.798 kb on - strand at 1243.803 kb on - strand at 1243.803 kb on - strand at 1243.808 kb on - strand at 1243.809 kb on + strand at 1243.809 kb on + strand at 1243.814 kb on + strand at 1243.814 kb on + strand at 1243.814 kb on + strand at 1243.830 kb on - strand at 1243.834 kb on + strand at 1243.843 kb on + strand at 1243.854 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 2g/L casaminos remove 1,241,432 + -2.0 1,241,433 - -2.4 1,241,434 + -1.5 1,241,485 - -0.5 1,241,488 - +0.8 1,241,489 + -0.2 1,241,489 + -2.9 1,241,491 - +1.1 1,241,503 - +1.3 1,241,503 - +1.7 1,241,504 + -1.0 1,241,504 + -0.9 1,241,506 - +0.2 1,241,526 - +0.3 1,241,526 - +1.1 1,241,527 + +1.0 1,241,527 + +0.8 1,241,527 + -0.8 1,241,527 + +1.0 1,241,529 - +1.8 1,241,529 - -0.1 1,241,531 - -0.2 1,241,531 - +0.8 1,241,532 + +1.4 1,241,532 + +0.8 1,241,556 - +1.0 1,241,614 - +1.9 1,241,617 + -0.8 1,241,617 + +0.2 1,241,628 - -0.1 1,241,634 - +1.3 1,241,635 + +0.8 1,241,635 + +0.5 1,241,658 - -1.8 1,241,664 + MMJJ_RS06720 0.10 -0.3 1,241,687 + MMJJ_RS06720 0.13 +1.5 1,241,690 + +1.3 1,241,734 + MMJJ_RS06720 0.19 -0.4 1,241,734 + MMJJ_RS06720 0.19 +0.2 1,241,754 - MMJJ_RS06720 0.21 +0.9 1,241,754 - MMJJ_RS06720 0.21 -1.1 1,241,755 + MMJJ_RS06720 0.22 -1.3 1,241,755 + MMJJ_RS06720 0.22 +0.7 1,241,771 - MMJJ_RS06720 0.24 +0.3 1,241,771 - MMJJ_RS06720 0.24 +0.5 1,241,771 - MMJJ_RS06720 0.24 +1.0 1,241,772 + MMJJ_RS06720 0.24 +1.0 1,241,776 + MMJJ_RS06720 0.24 -0.7 1,241,776 + MMJJ_RS06720 0.24 +0.8 1,241,781 - MMJJ_RS06720 0.25 +1.1 1,241,782 + MMJJ_RS06720 0.25 +0.1 1,241,785 + MMJJ_RS06720 0.25 -1.2 1,241,827 + MMJJ_RS06720 0.30 -0.2 1,241,888 + MMJJ_RS06720 0.38 -0.6 1,241,888 + MMJJ_RS06720 0.38 +1.4 1,241,921 + MMJJ_RS06720 0.42 -0.7 1,241,955 - MMJJ_RS06720 0.46 -0.4 1,241,955 - MMJJ_RS06720 0.46 +1.1 1,241,956 + MMJJ_RS06720 0.47 +1.4 1,242,020 + MMJJ_RS06720 0.54 +0.5 1,242,048 - MMJJ_RS06720 0.58 -0.3 1,242,048 - MMJJ_RS06720 0.58 -0.3 1,242,049 + MMJJ_RS06720 0.58 +0.8 1,242,064 - MMJJ_RS06720 0.60 -0.8 1,242,090 - MMJJ_RS06720 0.63 -0.4 1,242,122 - MMJJ_RS06720 0.67 +0.8 1,242,167 + MMJJ_RS06720 0.73 -0.7 1,242,187 + MMJJ_RS06720 0.75 +1.3 1,242,211 + MMJJ_RS06720 0.78 +1.5 1,242,216 - MMJJ_RS06720 0.79 +1.4 1,242,278 - MMJJ_RS06720 0.87 -0.0 1,242,302 + MMJJ_RS06720 0.90 +0.2 1,242,312 - +0.2 1,242,366 - +1.1 1,242,366 - +2.6 1,242,366 - +1.3 1,242,367 + +0.6 1,242,367 + -1.0 1,242,367 + +0.6 1,242,367 + +0.5 1,242,367 + +1.0 1,242,367 + +0.1 1,242,370 - +1.5 1,242,380 - -0.9 1,242,398 + -0.1 1,242,414 - -0.8 1,242,415 + -2.4 1,242,446 - -4.0 1,242,446 - -4.7 1,242,447 + -2.7 1,242,447 + -3.1 1,242,467 - MMJJ_RS06725 0.12 -3.3 1,242,468 + MMJJ_RS06725 0.12 -2.7 1,242,480 + MMJJ_RS06725 0.15 -2.8 1,242,480 + MMJJ_RS06725 0.15 -3.4 1,242,497 + MMJJ_RS06725 0.18 -3.9 1,242,500 - MMJJ_RS06725 0.19 -5.0 1,242,513 + MMJJ_RS06725 0.22 -2.8 1,242,514 - MMJJ_RS06725 0.22 -4.6 1,242,515 + MMJJ_RS06725 0.22 -3.4 1,242,550 - MMJJ_RS06725 0.29 -4.2 1,242,550 - MMJJ_RS06725 0.29 -3.3 1,242,551 + MMJJ_RS06725 0.29 -3.2 1,242,551 + MMJJ_RS06725 0.29 -1.1 1,242,565 - MMJJ_RS06725 0.32 -3.4 1,242,566 + MMJJ_RS06725 0.33 -2.6 1,242,570 - MMJJ_RS06725 0.33 -4.4 1,242,575 - MMJJ_RS06725 0.34 -2.3 1,242,575 - MMJJ_RS06725 0.34 -4.1 1,242,585 - MMJJ_RS06725 0.36 -3.5 1,242,586 + MMJJ_RS06725 0.37 -4.3 1,242,639 + MMJJ_RS06725 0.48 -4.1 1,242,641 - MMJJ_RS06725 0.48 -2.8 1,242,641 - MMJJ_RS06725 0.48 -5.1 1,242,641 - MMJJ_RS06725 0.48 -1.9 1,242,642 + MMJJ_RS06725 0.48 -2.3 1,242,650 - MMJJ_RS06725 0.50 -4.0 1,242,650 - MMJJ_RS06725 0.50 -1.8 1,242,675 - MMJJ_RS06725 0.55 -1.7 1,242,681 + MMJJ_RS06725 0.56 -0.4 1,242,681 + MMJJ_RS06725 0.56 -3.1 1,242,681 + MMJJ_RS06725 0.56 -2.7 1,242,681 + MMJJ_RS06725 0.56 -2.1 1,242,681 + MMJJ_RS06725 0.56 -2.0 1,242,696 + MMJJ_RS06725 0.59 -1.6 1,242,707 - MMJJ_RS06725 0.62 -1.7 1,242,707 - MMJJ_RS06725 0.62 -2.5 1,242,707 - MMJJ_RS06725 0.62 -4.6 1,242,708 + -0.3 1,242,708 + MMJJ_RS06725 0.62 -1.5 1,242,708 + MMJJ_RS06725 0.62 -1.8 1,242,708 + MMJJ_RS06725 0.62 -2.8 1,242,708 + MMJJ_RS06725 0.62 -2.8 1,242,708 + MMJJ_RS06725 0.62 -4.9 1,242,708 + MMJJ_RS06725 0.62 -1.7 1,242,717 - MMJJ_RS06725 0.64 -0.5 1,242,718 + MMJJ_RS06725 0.64 -2.6 1,242,738 + MMJJ_RS06725 0.68 -2.2 1,242,750 - MMJJ_RS06725 0.70 -3.8 1,242,750 - MMJJ_RS06725 0.70 -0.9 1,242,751 + MMJJ_RS06725 0.71 -3.0 1,242,771 - MMJJ_RS06725 0.75 -3.3 1,242,808 + MMJJ_RS06725 0.82 -3.5 1,242,808 + MMJJ_RS06725 0.82 -2.0 1,242,850 + -3.8 1,242,850 + -1.6 1,242,850 + -2.9 1,242,850 + -3.3 1,242,850 + -1.4 1,242,854 - -3.8 1,242,854 - -4.4 1,242,855 + -4.3 1,242,855 + -2.8 1,242,870 - -1.9 1,242,898 + -1.7 1,243,020 + MMJJ_RS06730 0.15 -0.6 1,243,033 - MMJJ_RS06730 0.16 -0.8 1,243,034 + MMJJ_RS06730 0.16 -0.1 1,243,097 - MMJJ_RS06730 0.24 -0.7 1,243,097 - MMJJ_RS06730 0.24 -0.0 1,243,105 + MMJJ_RS06730 0.25 -2.3 1,243,119 - MMJJ_RS06730 0.27 -2.6 1,243,120 + MMJJ_RS06730 0.27 -0.0 1,243,120 + MMJJ_RS06730 0.27 -0.5 1,243,122 + MMJJ_RS06730 0.27 -2.9 1,243,130 - MMJJ_RS06730 0.28 -0.8 1,243,130 - MMJJ_RS06730 0.28 -1.8 1,243,136 - MMJJ_RS06730 0.29 +0.9 1,243,137 + MMJJ_RS06730 0.29 -1.4 1,243,158 + MMJJ_RS06730 0.31 -3.0 1,243,178 - MMJJ_RS06730 0.34 +1.3 1,243,178 - MMJJ_RS06730 0.34 -0.9 1,243,314 + MMJJ_RS06730 0.50 -0.8 1,243,551 + MMJJ_RS06730 0.78 -2.6 1,243,701 - -0.0 1,243,701 - -0.1 1,243,702 + -2.9 1,243,706 - -1.3 1,243,752 - -1.8 1,243,752 - -1.1 1,243,752 - -1.8 1,243,752 - -1.1 1,243,752 - -0.3 1,243,753 + -0.8 1,243,753 + -1.1 1,243,753 + -1.4 1,243,753 + -2.7 1,243,753 + -0.6 1,243,753 + -2.6 1,243,753 + -0.0 1,243,753 + +0.4 1,243,753 + -0.8 1,243,753 + +0.2 1,243,753 + +0.5 1,243,754 - +0.2 1,243,754 - +0.6 1,243,754 - +0.4 1,243,761 + -1.3 1,243,766 - -0.4 1,243,767 + -0.8 1,243,785 - +1.6 1,243,798 - +0.2 1,243,803 - +0.9 1,243,803 - +0.7 1,243,808 - -1.6 1,243,809 + -0.5 1,243,809 + -1.1 1,243,814 + +0.0 1,243,814 + +0.4 1,243,814 + +0.5 1,243,830 - +0.2 1,243,834 + -0.0 1,243,843 + +0.4 1,243,854 - -1.7
Or see this region's nucleotide sequence