Experiment: H2 N-free with 2g/L casaminos
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS06685 and MMJJ_RS06690 are separated by 105 nucleotides MMJJ_RS06690 and MMJJ_RS06695 are separated by 45 nucleotides MMJJ_RS06695 and MMJJ_RS06700 are separated by 2 nucleotides MMJJ_RS06700 and MMJJ_RS06705 are separated by 91 nucleotides
MMJJ_RS06685: MMJJ_RS06685 - S-layer protein, at 1,232,554 to 1,233,894
_RS06685
MMJJ_RS06690: MMJJ_RS06690 - orotate phosphoribosyltransferase, at 1,234,000 to 1,234,557
_RS06690
MMJJ_RS06695: MMJJ_RS06695 - amidohydrolase, at 1,234,603 to 1,235,871
_RS06695
MMJJ_RS06700: MMJJ_RS06700 - L-seryl-tRNA(Sec) kinase, at 1,235,874 to 1,236,641
_RS06700
MMJJ_RS06705: MMJJ_RS06705 - bifunctional NADP phosphatase/NAD kinase, at 1,236,733 to 1,238,433
_RS06705
Position (kb)
1234
1235
1236 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1233.677 kb on - strand, within MMJJ_RS06685 at 1233.677 kb on - strand, within MMJJ_RS06685 at 1233.678 kb on + strand, within MMJJ_RS06685 at 1233.682 kb on + strand, within MMJJ_RS06685 at 1233.684 kb on - strand, within MMJJ_RS06685 at 1233.685 kb on + strand, within MMJJ_RS06685 at 1233.743 kb on - strand, within MMJJ_RS06685 at 1233.744 kb on + strand, within MMJJ_RS06685 at 1233.748 kb on + strand, within MMJJ_RS06685 at 1233.802 kb on - strand at 1233.802 kb on - strand at 1233.803 kb on + strand at 1233.815 kb on + strand at 1233.815 kb on + strand at 1233.815 kb on + strand at 1233.815 kb on + strand at 1233.822 kb on - strand at 1233.827 kb on - strand at 1233.827 kb on - strand at 1233.832 kb on - strand at 1233.851 kb on + strand at 1233.851 kb on + strand at 1233.857 kb on + strand at 1233.861 kb on - strand at 1233.861 kb on - strand at 1233.862 kb on + strand at 1233.865 kb on - strand at 1233.865 kb on - strand at 1233.866 kb on + strand at 1233.866 kb on + strand at 1233.866 kb on + strand at 1233.866 kb on + strand at 1233.908 kb on + strand at 1233.924 kb on - strand at 1233.924 kb on - strand at 1233.926 kb on - strand at 1233.926 kb on - strand at 1233.927 kb on + strand at 1233.927 kb on + strand at 1233.927 kb on + strand at 1233.931 kb on + strand at 1233.931 kb on + strand at 1233.931 kb on + strand at 1233.931 kb on + strand at 1233.931 kb on + strand at 1233.942 kb on + strand at 1233.942 kb on + strand at 1233.942 kb on + strand at 1233.943 kb on - strand at 1233.943 kb on - strand at 1233.944 kb on + strand at 1233.944 kb on + strand at 1233.944 kb on + strand at 1233.944 kb on + strand at 1233.944 kb on + strand at 1233.944 kb on + strand at 1233.944 kb on + strand at 1233.944 kb on + strand at 1233.944 kb on + strand at 1233.955 kb on + strand at 1233.964 kb on + strand at 1233.964 kb on + strand at 1233.964 kb on + strand at 1234.058 kb on - strand, within MMJJ_RS06690 at 1234.123 kb on + strand, within MMJJ_RS06690 at 1234.551 kb on - strand at 1234.566 kb on - strand at 1234.578 kb on + strand at 1234.952 kb on + strand, within MMJJ_RS06695 at 1235.146 kb on + strand, within MMJJ_RS06695 at 1235.172 kb on - strand, within MMJJ_RS06695 at 1235.281 kb on - strand, within MMJJ_RS06695 at 1235.364 kb on + strand at 1235.382 kb on + strand, within MMJJ_RS06695 at 1235.406 kb on - strand, within MMJJ_RS06695 at 1235.497 kb on + strand, within MMJJ_RS06695 at 1235.746 kb on + strand at 1235.769 kb on + strand at 1235.851 kb on - strand at 1235.852 kb on + strand at 1235.852 kb on + strand at 1235.896 kb on - strand at 1235.901 kb on - strand at 1236.086 kb on - strand, within MMJJ_RS06700 at 1236.104 kb on - strand, within MMJJ_RS06700 at 1236.107 kb on - strand, within MMJJ_RS06700 at 1236.107 kb on - strand, within MMJJ_RS06700 at 1236.108 kb on + strand, within MMJJ_RS06700 at 1236.204 kb on + strand, within MMJJ_RS06700 at 1236.211 kb on + strand, within MMJJ_RS06700 at 1236.223 kb on - strand, within MMJJ_RS06700 at 1236.253 kb on - strand, within MMJJ_RS06700 at 1236.254 kb on + strand, within MMJJ_RS06700 at 1236.339 kb on - strand at 1236.340 kb on + strand, within MMJJ_RS06700 at 1236.340 kb on + strand, within MMJJ_RS06700 at 1236.528 kb on - strand, within MMJJ_RS06700 at 1236.528 kb on - strand, within MMJJ_RS06700 at 1236.636 kb on - strand at 1236.636 kb on - strand at 1236.660 kb on + strand at 1236.671 kb on - strand at 1236.672 kb on + strand at 1236.693 kb on - strand at 1236.694 kb on + strand at 1236.694 kb on + strand at 1236.697 kb on - strand at 1236.697 kb on - strand at 1236.699 kb on - strand at 1236.702 kb on - strand at 1236.706 kb on - strand at 1236.709 kb on - strand at 1236.709 kb on - strand at 1236.709 kb on - strand at 1236.709 kb on - strand at 1236.710 kb on + strand at 1236.710 kb on + strand at 1236.713 kb on - strand at 1236.713 kb on - strand at 1236.713 kb on - strand at 1236.713 kb on - strand at 1236.713 kb on - strand at 1236.713 kb on - strand at 1236.713 kb on - strand at 1236.714 kb on + strand at 1236.714 kb on + strand at 1236.714 kb on + strand at 1236.717 kb on - strand at 1236.717 kb on - strand at 1236.720 kb on - strand at 1236.720 kb on - strand at 1236.756 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 2g/L casaminos remove 1,233,677 - MMJJ_RS06685 0.84 +0.6 1,233,677 - MMJJ_RS06685 0.84 +0.2 1,233,678 + MMJJ_RS06685 0.84 +0.9 1,233,682 + MMJJ_RS06685 0.84 -1.9 1,233,684 - MMJJ_RS06685 0.84 -1.0 1,233,685 + MMJJ_RS06685 0.84 -1.9 1,233,743 - MMJJ_RS06685 0.89 +0.1 1,233,744 + MMJJ_RS06685 0.89 -0.4 1,233,748 + MMJJ_RS06685 0.89 -0.3 1,233,802 - -0.2 1,233,802 - +0.7 1,233,803 + +1.1 1,233,815 + -0.6 1,233,815 + +1.2 1,233,815 + +1.4 1,233,815 + +1.0 1,233,822 - -0.9 1,233,827 - -0.3 1,233,827 - +0.3 1,233,832 - -0.0 1,233,851 + +0.0 1,233,851 + -0.8 1,233,857 + +0.3 1,233,861 - +0.6 1,233,861 - -1.2 1,233,862 + -0.1 1,233,865 - +0.3 1,233,865 - +1.5 1,233,866 + -0.1 1,233,866 + +0.1 1,233,866 + -0.9 1,233,866 + +0.3 1,233,908 + +0.2 1,233,924 - +0.7 1,233,924 - +0.9 1,233,926 - -2.4 1,233,926 - -0.8 1,233,927 + +0.3 1,233,927 + -0.2 1,233,927 + +1.2 1,233,931 + +0.4 1,233,931 + -1.6 1,233,931 + -0.3 1,233,931 + -0.5 1,233,931 + +0.3 1,233,942 + +0.6 1,233,942 + +1.0 1,233,942 + +0.7 1,233,943 - -1.5 1,233,943 - -0.7 1,233,944 + +0.5 1,233,944 + -1.9 1,233,944 + -0.8 1,233,944 + +0.4 1,233,944 + -1.5 1,233,944 + -1.7 1,233,944 + +1.0 1,233,944 + +0.3 1,233,944 + +0.9 1,233,955 + +1.2 1,233,964 + -1.3 1,233,964 + +0.6 1,233,964 + -1.0 1,234,058 - MMJJ_RS06690 0.10 -1.3 1,234,123 + MMJJ_RS06690 0.22 +0.3 1,234,551 - +0.8 1,234,566 - +0.4 1,234,578 + -0.6 1,234,952 + MMJJ_RS06695 0.28 -0.8 1,235,146 + MMJJ_RS06695 0.43 -2.2 1,235,172 - MMJJ_RS06695 0.45 -2.7 1,235,281 - MMJJ_RS06695 0.53 -1.9 1,235,364 + -0.7 1,235,382 + MMJJ_RS06695 0.61 -2.1 1,235,406 - MMJJ_RS06695 0.63 -2.1 1,235,497 + MMJJ_RS06695 0.70 -2.4 1,235,746 + -1.7 1,235,769 + -1.7 1,235,851 - +0.7 1,235,852 + +0.1 1,235,852 + +1.6 1,235,896 - -3.7 1,235,901 - -2.9 1,236,086 - MMJJ_RS06700 0.28 -1.8 1,236,104 - MMJJ_RS06700 0.30 -3.5 1,236,107 - MMJJ_RS06700 0.30 +0.3 1,236,107 - MMJJ_RS06700 0.30 -1.2 1,236,108 + MMJJ_RS06700 0.30 -2.3 1,236,204 + MMJJ_RS06700 0.43 -2.9 1,236,211 + MMJJ_RS06700 0.44 -2.8 1,236,223 - MMJJ_RS06700 0.45 -1.5 1,236,253 - MMJJ_RS06700 0.49 -1.5 1,236,254 + MMJJ_RS06700 0.49 -2.5 1,236,339 - -1.3 1,236,340 + MMJJ_RS06700 0.61 -2.1 1,236,340 + MMJJ_RS06700 0.61 -1.5 1,236,528 - MMJJ_RS06700 0.85 -1.3 1,236,528 - MMJJ_RS06700 0.85 -3.6 1,236,636 - -1.2 1,236,636 - -3.1 1,236,660 + -0.1 1,236,671 - -0.5 1,236,672 + +1.0 1,236,693 - +0.9 1,236,694 + +1.0 1,236,694 + +0.4 1,236,697 - +0.7 1,236,697 - -0.8 1,236,699 - +0.7 1,236,702 - +1.0 1,236,706 - +0.0 1,236,709 - +1.2 1,236,709 - +1.4 1,236,709 - +0.3 1,236,709 - -0.3 1,236,710 + +0.8 1,236,710 + -1.7 1,236,713 - +0.8 1,236,713 - +1.0 1,236,713 - +0.2 1,236,713 - +0.1 1,236,713 - -0.6 1,236,713 - -1.9 1,236,713 - +0.8 1,236,714 + +0.9 1,236,714 + -1.9 1,236,714 + +1.1 1,236,717 - +0.9 1,236,717 - +1.5 1,236,720 - -0.3 1,236,720 - +0.6 1,236,756 + -2.2
Or see this region's nucleotide sequence