Experiment: H2 N-free with 2g/L casaminos
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS06640 and MMJJ_RS06650 are separated by 568 nucleotides MMJJ_RS06650 and MMJJ_RS06655 are separated by 48 nucleotides MMJJ_RS06655 and MMJJ_RS06660 are separated by 6 nucleotides
MMJJ_RS06640: MMJJ_RS06640 - YfcE family phosphodiesterase, at 1,225,245 to 1,225,736
_RS06640
MMJJ_RS06650: MMJJ_RS06650 - cell division protein FtsZ, at 1,226,305 to 1,227,402
_RS06650
MMJJ_RS06655: MMJJ_RS06655 - transcriptional regulator, at 1,227,451 to 1,227,963
_RS06655
MMJJ_RS06660: MMJJ_RS06660 - DUF1188 domain-containing protein, at 1,227,970 to 1,228,752
_RS06660
Position (kb)
1226
1227
1228 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1225.357 kb on + strand, within MMJJ_RS06640 at 1225.377 kb on - strand, within MMJJ_RS06640 at 1225.377 kb on - strand, within MMJJ_RS06640 at 1225.377 kb on - strand at 1225.378 kb on + strand, within MMJJ_RS06640 at 1225.378 kb on + strand, within MMJJ_RS06640 at 1225.378 kb on + strand, within MMJJ_RS06640 at 1225.386 kb on - strand, within MMJJ_RS06640 at 1225.386 kb on - strand, within MMJJ_RS06640 at 1225.386 kb on - strand, within MMJJ_RS06640 at 1225.391 kb on - strand, within MMJJ_RS06640 at 1225.391 kb on - strand, within MMJJ_RS06640 at 1225.391 kb on - strand, within MMJJ_RS06640 at 1225.392 kb on + strand, within MMJJ_RS06640 at 1225.392 kb on + strand, within MMJJ_RS06640 at 1225.403 kb on - strand, within MMJJ_RS06640 at 1225.404 kb on + strand, within MMJJ_RS06640 at 1225.404 kb on + strand, within MMJJ_RS06640 at 1225.444 kb on + strand, within MMJJ_RS06640 at 1225.448 kb on + strand, within MMJJ_RS06640 at 1225.463 kb on - strand, within MMJJ_RS06640 at 1225.463 kb on - strand, within MMJJ_RS06640 at 1225.463 kb on - strand, within MMJJ_RS06640 at 1225.464 kb on + strand, within MMJJ_RS06640 at 1225.464 kb on + strand, within MMJJ_RS06640 at 1225.464 kb on + strand, within MMJJ_RS06640 at 1225.524 kb on - strand, within MMJJ_RS06640 at 1225.524 kb on - strand, within MMJJ_RS06640 at 1225.524 kb on - strand, within MMJJ_RS06640 at 1225.524 kb on - strand, within MMJJ_RS06640 at 1225.550 kb on - strand, within MMJJ_RS06640 at 1225.552 kb on - strand, within MMJJ_RS06640 at 1225.552 kb on - strand, within MMJJ_RS06640 at 1225.553 kb on + strand, within MMJJ_RS06640 at 1225.623 kb on - strand, within MMJJ_RS06640 at 1225.624 kb on + strand, within MMJJ_RS06640 at 1225.707 kb on + strand at 1225.728 kb on - strand at 1225.728 kb on - strand at 1225.730 kb on - strand at 1225.770 kb on - strand at 1225.771 kb on + strand at 1225.774 kb on - strand at 1226.117 kb on + strand at 1226.125 kb on + strand at 1226.127 kb on + strand at 1226.134 kb on - strand at 1226.134 kb on - strand at 1226.139 kb on - strand at 1226.140 kb on + strand at 1226.144 kb on - strand at 1226.148 kb on + strand at 1226.148 kb on + strand at 1226.154 kb on - strand at 1226.155 kb on + strand at 1226.158 kb on - strand at 1226.158 kb on - strand at 1226.160 kb on - strand at 1226.161 kb on + strand at 1226.161 kb on + strand at 1226.163 kb on - strand at 1226.163 kb on - strand at 1226.188 kb on - strand at 1226.188 kb on - strand at 1226.188 kb on - strand at 1226.196 kb on + strand at 1226.198 kb on - strand at 1226.199 kb on + strand at 1226.199 kb on + strand at 1226.201 kb on + strand at 1226.201 kb on + strand at 1226.201 kb on + strand at 1226.202 kb on - strand at 1226.202 kb on - strand at 1226.203 kb on + strand at 1226.207 kb on - strand at 1226.221 kb on - strand at 1226.221 kb on - strand at 1226.222 kb on + strand at 1226.225 kb on - strand at 1226.254 kb on - strand at 1226.254 kb on - strand at 1226.255 kb on + strand at 1226.257 kb on - strand at 1226.257 kb on - strand at 1226.258 kb on + strand at 1226.260 kb on - strand at 1226.267 kb on + strand at 1226.267 kb on + strand at 1226.272 kb on + strand at 1226.272 kb on + strand at 1226.301 kb on - strand at 1226.304 kb on - strand at 1226.304 kb on - strand at 1226.315 kb on - strand at 1226.315 kb on - strand at 1226.350 kb on - strand at 1226.350 kb on - strand at 1226.351 kb on + strand at 1226.351 kb on + strand at 1226.351 kb on + strand at 1226.427 kb on - strand, within MMJJ_RS06650 at 1226.454 kb on - strand, within MMJJ_RS06650 at 1226.455 kb on + strand, within MMJJ_RS06650 at 1226.481 kb on - strand, within MMJJ_RS06650 at 1226.485 kb on + strand, within MMJJ_RS06650 at 1226.501 kb on - strand, within MMJJ_RS06650 at 1226.501 kb on - strand, within MMJJ_RS06650 at 1226.501 kb on - strand, within MMJJ_RS06650 at 1226.501 kb on - strand, within MMJJ_RS06650 at 1226.502 kb on + strand, within MMJJ_RS06650 at 1226.510 kb on - strand, within MMJJ_RS06650 at 1226.531 kb on - strand, within MMJJ_RS06650 at 1226.645 kb on - strand, within MMJJ_RS06650 at 1226.703 kb on + strand, within MMJJ_RS06650 at 1226.732 kb on - strand, within MMJJ_RS06650 at 1226.733 kb on + strand, within MMJJ_RS06650 at 1226.733 kb on + strand, within MMJJ_RS06650 at 1226.753 kb on + strand, within MMJJ_RS06650 at 1226.803 kb on + strand, within MMJJ_RS06650 at 1226.811 kb on - strand, within MMJJ_RS06650 at 1226.811 kb on - strand, within MMJJ_RS06650 at 1226.812 kb on + strand, within MMJJ_RS06650 at 1226.826 kb on - strand, within MMJJ_RS06650 at 1226.832 kb on - strand, within MMJJ_RS06650 at 1226.832 kb on - strand, within MMJJ_RS06650 at 1226.837 kb on - strand, within MMJJ_RS06650 at 1226.837 kb on - strand, within MMJJ_RS06650 at 1226.837 kb on - strand, within MMJJ_RS06650 at 1226.876 kb on - strand, within MMJJ_RS06650 at 1226.876 kb on - strand, within MMJJ_RS06650 at 1226.877 kb on + strand, within MMJJ_RS06650 at 1226.900 kb on - strand, within MMJJ_RS06650 at 1226.900 kb on - strand, within MMJJ_RS06650 at 1226.914 kb on + strand, within MMJJ_RS06650 at 1227.043 kb on - strand, within MMJJ_RS06650 at 1227.080 kb on - strand, within MMJJ_RS06650 at 1227.085 kb on + strand, within MMJJ_RS06650 at 1227.089 kb on - strand, within MMJJ_RS06650 at 1227.089 kb on - strand, within MMJJ_RS06650 at 1227.089 kb on - strand, within MMJJ_RS06650 at 1227.089 kb on - strand, within MMJJ_RS06650 at 1227.090 kb on + strand, within MMJJ_RS06650 at 1227.090 kb on + strand, within MMJJ_RS06650 at 1227.104 kb on - strand, within MMJJ_RS06650 at 1227.104 kb on - strand, within MMJJ_RS06650 at 1227.104 kb on - strand, within MMJJ_RS06650 at 1227.140 kb on - strand, within MMJJ_RS06650 at 1227.140 kb on - strand, within MMJJ_RS06650 at 1227.141 kb on + strand, within MMJJ_RS06650 at 1227.147 kb on + strand, within MMJJ_RS06650 at 1227.147 kb on + strand, within MMJJ_RS06650 at 1227.147 kb on + strand, within MMJJ_RS06650 at 1227.147 kb on + strand, within MMJJ_RS06650 at 1227.147 kb on + strand, within MMJJ_RS06650 at 1227.164 kb on - strand, within MMJJ_RS06650 at 1227.180 kb on - strand, within MMJJ_RS06650 at 1227.180 kb on - strand, within MMJJ_RS06650 at 1227.180 kb on - strand, within MMJJ_RS06650 at 1227.219 kb on + strand, within MMJJ_RS06650 at 1227.231 kb on - strand, within MMJJ_RS06650 at 1227.231 kb on - strand, within MMJJ_RS06650 at 1227.288 kb on - strand, within MMJJ_RS06650 at 1227.291 kb on - strand, within MMJJ_RS06650 at 1227.312 kb on - strand at 1227.313 kb on + strand at 1227.314 kb on - strand at 1227.392 kb on + strand at 1227.392 kb on + strand at 1227.393 kb on - strand at 1227.394 kb on + strand at 1227.399 kb on + strand at 1227.399 kb on + strand at 1227.399 kb on + strand at 1227.432 kb on - strand at 1227.439 kb on - strand at 1227.440 kb on + strand at 1227.450 kb on - strand at 1227.451 kb on + strand at 1227.451 kb on + strand at 1227.451 kb on + strand at 1227.451 kb on + strand at 1227.451 kb on + strand at 1227.492 kb on - strand at 1227.543 kb on + strand, within MMJJ_RS06655 at 1227.561 kb on + strand, within MMJJ_RS06655 at 1227.561 kb on + strand, within MMJJ_RS06655 at 1227.576 kb on - strand, within MMJJ_RS06655 at 1227.577 kb on + strand, within MMJJ_RS06655 at 1227.577 kb on + strand, within MMJJ_RS06655 at 1227.577 kb on + strand, within MMJJ_RS06655 at 1227.580 kb on + strand, within MMJJ_RS06655 at 1227.586 kb on - strand, within MMJJ_RS06655 at 1227.586 kb on - strand, within MMJJ_RS06655 at 1227.589 kb on + strand, within MMJJ_RS06655 at 1227.637 kb on + strand, within MMJJ_RS06655 at 1227.682 kb on + strand, within MMJJ_RS06655 at 1227.686 kb on - strand, within MMJJ_RS06655 at 1227.687 kb on + strand, within MMJJ_RS06655 at 1227.687 kb on + strand, within MMJJ_RS06655 at 1227.713 kb on - strand at 1227.723 kb on + strand, within MMJJ_RS06655 at 1227.799 kb on + strand, within MMJJ_RS06655 at 1227.812 kb on - strand, within MMJJ_RS06655 at 1227.814 kb on - strand, within MMJJ_RS06655 at 1227.814 kb on - strand, within MMJJ_RS06655 at 1227.814 kb on - strand, within MMJJ_RS06655 at 1227.858 kb on + strand, within MMJJ_RS06655 at 1227.861 kb on + strand, within MMJJ_RS06655 at 1227.864 kb on - strand, within MMJJ_RS06655 at 1227.884 kb on - strand, within MMJJ_RS06655 at 1227.885 kb on + strand, within MMJJ_RS06655 at 1227.891 kb on + strand, within MMJJ_RS06655 at 1227.891 kb on + strand, within MMJJ_RS06655 at 1227.907 kb on + strand, within MMJJ_RS06655 at 1227.972 kb on + strand at 1227.994 kb on - strand at 1227.994 kb on - strand at 1227.995 kb on + strand at 1227.995 kb on + strand at 1228.005 kb on - strand at 1228.019 kb on + strand at 1228.064 kb on + strand, within MMJJ_RS06660 at 1228.133 kb on - strand, within MMJJ_RS06660 at 1228.134 kb on + strand, within MMJJ_RS06660 at 1228.164 kb on - strand, within MMJJ_RS06660 at 1228.169 kb on + strand, within MMJJ_RS06660 at 1228.169 kb on + strand, within MMJJ_RS06660 at 1228.169 kb on + strand, within MMJJ_RS06660 at 1228.201 kb on - strand, within MMJJ_RS06660 at 1228.312 kb on - strand, within MMJJ_RS06660
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 2g/L casaminos remove 1,225,357 + MMJJ_RS06640 0.23 +0.3 1,225,377 - MMJJ_RS06640 0.27 -1.7 1,225,377 - MMJJ_RS06640 0.27 +0.6 1,225,377 - -0.7 1,225,378 + MMJJ_RS06640 0.27 +1.1 1,225,378 + MMJJ_RS06640 0.27 -0.8 1,225,378 + MMJJ_RS06640 0.27 +1.8 1,225,386 - MMJJ_RS06640 0.29 +0.3 1,225,386 - MMJJ_RS06640 0.29 +1.0 1,225,386 - MMJJ_RS06640 0.29 +1.4 1,225,391 - MMJJ_RS06640 0.30 -0.0 1,225,391 - MMJJ_RS06640 0.30 +1.1 1,225,391 - MMJJ_RS06640 0.30 +0.9 1,225,392 + MMJJ_RS06640 0.30 -0.3 1,225,392 + MMJJ_RS06640 0.30 -0.0 1,225,403 - MMJJ_RS06640 0.32 -0.3 1,225,404 + MMJJ_RS06640 0.32 -1.5 1,225,404 + MMJJ_RS06640 0.32 -2.3 1,225,444 + MMJJ_RS06640 0.40 +1.6 1,225,448 + MMJJ_RS06640 0.41 +0.0 1,225,463 - MMJJ_RS06640 0.44 +0.4 1,225,463 - MMJJ_RS06640 0.44 +0.9 1,225,463 - MMJJ_RS06640 0.44 +0.6 1,225,464 + MMJJ_RS06640 0.45 +0.4 1,225,464 + MMJJ_RS06640 0.45 +1.7 1,225,464 + MMJJ_RS06640 0.45 -1.2 1,225,524 - MMJJ_RS06640 0.57 -0.7 1,225,524 - MMJJ_RS06640 0.57 -0.4 1,225,524 - MMJJ_RS06640 0.57 +0.1 1,225,524 - MMJJ_RS06640 0.57 +0.4 1,225,550 - MMJJ_RS06640 0.62 +0.7 1,225,552 - MMJJ_RS06640 0.62 -1.4 1,225,552 - MMJJ_RS06640 0.62 +0.4 1,225,553 + MMJJ_RS06640 0.63 +0.7 1,225,623 - MMJJ_RS06640 0.77 +1.1 1,225,624 + MMJJ_RS06640 0.77 +0.6 1,225,707 + -0.1 1,225,728 - -1.7 1,225,728 - +0.2 1,225,730 - -2.8 1,225,770 - -0.3 1,225,771 + +0.6 1,225,774 - +0.9 1,226,117 + +0.3 1,226,125 + -0.1 1,226,127 + -0.7 1,226,134 - -1.6 1,226,134 - -1.7 1,226,139 - +0.8 1,226,140 + +1.6 1,226,144 - +1.5 1,226,148 + -0.1 1,226,148 + -0.8 1,226,154 - +0.9 1,226,155 + -0.4 1,226,158 - +0.1 1,226,158 - +0.1 1,226,160 - +0.3 1,226,161 + +0.9 1,226,161 + +0.5 1,226,163 - +0.5 1,226,163 - +1.2 1,226,188 - +0.0 1,226,188 - +1.0 1,226,188 - +1.5 1,226,196 + +0.5 1,226,198 - +0.6 1,226,199 + +0.3 1,226,199 + +0.2 1,226,201 + -1.5 1,226,201 + -1.8 1,226,201 + -1.3 1,226,202 - -2.2 1,226,202 - -1.4 1,226,203 + -2.2 1,226,207 - -2.1 1,226,221 - +0.7 1,226,221 - -2.1 1,226,222 + -2.5 1,226,225 - -1.2 1,226,254 - +0.2 1,226,254 - -0.9 1,226,255 + -0.0 1,226,257 - -0.4 1,226,257 - -4.1 1,226,258 + -1.6 1,226,260 - -2.3 1,226,267 + -1.7 1,226,267 + -2.6 1,226,272 + -0.2 1,226,272 + -1.2 1,226,301 - -4.3 1,226,304 - -3.1 1,226,304 - -1.3 1,226,315 - -1.3 1,226,315 - -2.3 1,226,350 - +0.6 1,226,350 - -1.5 1,226,351 + -2.7 1,226,351 + -3.0 1,226,351 + -1.7 1,226,427 - MMJJ_RS06650 0.11 -0.8 1,226,454 - MMJJ_RS06650 0.14 -3.9 1,226,455 + MMJJ_RS06650 0.14 -0.8 1,226,481 - MMJJ_RS06650 0.16 -1.9 1,226,485 + MMJJ_RS06650 0.16 -1.8 1,226,501 - MMJJ_RS06650 0.18 -1.0 1,226,501 - MMJJ_RS06650 0.18 +0.1 1,226,501 - MMJJ_RS06650 0.18 -3.6 1,226,501 - MMJJ_RS06650 0.18 -2.5 1,226,502 + MMJJ_RS06650 0.18 -1.8 1,226,510 - MMJJ_RS06650 0.19 -2.3 1,226,531 - MMJJ_RS06650 0.21 -2.8 1,226,645 - MMJJ_RS06650 0.31 -0.1 1,226,703 + MMJJ_RS06650 0.36 -3.6 1,226,732 - MMJJ_RS06650 0.39 -2.2 1,226,733 + MMJJ_RS06650 0.39 -2.3 1,226,733 + MMJJ_RS06650 0.39 -3.2 1,226,753 + MMJJ_RS06650 0.41 -4.2 1,226,803 + MMJJ_RS06650 0.45 -2.1 1,226,811 - MMJJ_RS06650 0.46 -2.2 1,226,811 - MMJJ_RS06650 0.46 -1.2 1,226,812 + MMJJ_RS06650 0.46 -0.7 1,226,826 - MMJJ_RS06650 0.47 -2.0 1,226,832 - MMJJ_RS06650 0.48 +1.1 1,226,832 - MMJJ_RS06650 0.48 -2.1 1,226,837 - MMJJ_RS06650 0.48 -1.9 1,226,837 - MMJJ_RS06650 0.48 -1.0 1,226,837 - MMJJ_RS06650 0.48 -4.1 1,226,876 - MMJJ_RS06650 0.52 -3.0 1,226,876 - MMJJ_RS06650 0.52 -1.4 1,226,877 + MMJJ_RS06650 0.52 -2.5 1,226,900 - MMJJ_RS06650 0.54 -3.7 1,226,900 - MMJJ_RS06650 0.54 -2.1 1,226,914 + MMJJ_RS06650 0.55 -1.0 1,227,043 - MMJJ_RS06650 0.67 -1.8 1,227,080 - MMJJ_RS06650 0.71 -1.1 1,227,085 + MMJJ_RS06650 0.71 -1.0 1,227,089 - MMJJ_RS06650 0.71 -3.6 1,227,089 - MMJJ_RS06650 0.71 -2.1 1,227,089 - MMJJ_RS06650 0.71 -3.0 1,227,089 - MMJJ_RS06650 0.71 -2.4 1,227,090 + MMJJ_RS06650 0.71 -2.4 1,227,090 + MMJJ_RS06650 0.71 -2.6 1,227,104 - MMJJ_RS06650 0.73 -0.6 1,227,104 - MMJJ_RS06650 0.73 -2.5 1,227,104 - MMJJ_RS06650 0.73 -1.0 1,227,140 - MMJJ_RS06650 0.76 -2.4 1,227,140 - MMJJ_RS06650 0.76 -0.8 1,227,141 + MMJJ_RS06650 0.76 -1.9 1,227,147 + MMJJ_RS06650 0.77 -0.4 1,227,147 + MMJJ_RS06650 0.77 -1.7 1,227,147 + MMJJ_RS06650 0.77 -1.5 1,227,147 + MMJJ_RS06650 0.77 -3.5 1,227,147 + MMJJ_RS06650 0.77 -4.0 1,227,164 - MMJJ_RS06650 0.78 -2.4 1,227,180 - MMJJ_RS06650 0.80 -1.4 1,227,180 - MMJJ_RS06650 0.80 -1.4 1,227,180 - MMJJ_RS06650 0.80 -0.8 1,227,219 + MMJJ_RS06650 0.83 -2.8 1,227,231 - MMJJ_RS06650 0.84 -1.8 1,227,231 - MMJJ_RS06650 0.84 -2.7 1,227,288 - MMJJ_RS06650 0.90 -0.9 1,227,291 - MMJJ_RS06650 0.90 -2.4 1,227,312 - -2.1 1,227,313 + -2.0 1,227,314 - -1.0 1,227,392 + -2.5 1,227,392 + -2.8 1,227,393 - -1.8 1,227,394 + -2.0 1,227,399 + -1.3 1,227,399 + -1.7 1,227,399 + -0.9 1,227,432 - -2.0 1,227,439 - -3.8 1,227,440 + -4.0 1,227,450 - -2.7 1,227,451 + -2.2 1,227,451 + -1.0 1,227,451 + -2.1 1,227,451 + -1.3 1,227,451 + -1.5 1,227,492 - -1.0 1,227,543 + MMJJ_RS06655 0.18 -1.7 1,227,561 + MMJJ_RS06655 0.21 -2.0 1,227,561 + MMJJ_RS06655 0.21 +0.5 1,227,576 - MMJJ_RS06655 0.24 -2.2 1,227,577 + MMJJ_RS06655 0.25 -1.4 1,227,577 + MMJJ_RS06655 0.25 -3.6 1,227,577 + MMJJ_RS06655 0.25 -1.5 1,227,580 + MMJJ_RS06655 0.25 -1.5 1,227,586 - MMJJ_RS06655 0.26 -2.3 1,227,586 - MMJJ_RS06655 0.26 -0.3 1,227,589 + MMJJ_RS06655 0.27 -1.6 1,227,637 + MMJJ_RS06655 0.36 -3.1 1,227,682 + MMJJ_RS06655 0.45 -1.9 1,227,686 - MMJJ_RS06655 0.46 -1.3 1,227,687 + MMJJ_RS06655 0.46 -2.8 1,227,687 + MMJJ_RS06655 0.46 -1.9 1,227,713 - -1.0 1,227,723 + MMJJ_RS06655 0.53 -4.3 1,227,799 + MMJJ_RS06655 0.68 -1.9 1,227,812 - MMJJ_RS06655 0.70 -2.5 1,227,814 - MMJJ_RS06655 0.71 -1.8 1,227,814 - MMJJ_RS06655 0.71 -0.9 1,227,814 - MMJJ_RS06655 0.71 -0.9 1,227,858 + MMJJ_RS06655 0.79 -1.3 1,227,861 + MMJJ_RS06655 0.80 -0.6 1,227,864 - MMJJ_RS06655 0.81 +0.1 1,227,884 - MMJJ_RS06655 0.84 -1.3 1,227,885 + MMJJ_RS06655 0.85 +0.1 1,227,891 + MMJJ_RS06655 0.86 -2.4 1,227,891 + MMJJ_RS06655 0.86 -2.2 1,227,907 + MMJJ_RS06655 0.89 +0.4 1,227,972 + -3.2 1,227,994 - -1.5 1,227,994 - -1.3 1,227,995 + +0.2 1,227,995 + +0.5 1,228,005 - +0.1 1,228,019 + +0.0 1,228,064 + MMJJ_RS06660 0.12 +0.2 1,228,133 - MMJJ_RS06660 0.21 +0.4 1,228,134 + MMJJ_RS06660 0.21 -1.2 1,228,164 - MMJJ_RS06660 0.25 -2.6 1,228,169 + MMJJ_RS06660 0.25 -0.7 1,228,169 + MMJJ_RS06660 0.25 -1.7 1,228,169 + MMJJ_RS06660 0.25 -0.5 1,228,201 - MMJJ_RS06660 0.30 +0.7 1,228,312 - MMJJ_RS06660 0.44 -0.2
Or see this region's nucleotide sequence