Experiment: H2 N-free with 2g/L casaminos
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS06380 and MMJJ_RS06385 are separated by 137 nucleotides MMJJ_RS06385 and MMJJ_RS06390 overlap by 4 nucleotides MMJJ_RS06390 and MMJJ_RS06395 are separated by 21 nucleotides MMJJ_RS06395 and MMJJ_RS06400 are separated by 77 nucleotides
MMJJ_RS06380: MMJJ_RS06380 - signal recognition particle protein Srp54, at 1,181,565 to 1,182,917
_RS06380
MMJJ_RS06385: MMJJ_RS06385 - hypothetical protein, at 1,183,055 to 1,183,276
_RS06385
MMJJ_RS06390: MMJJ_RS06390 - NADPH-dependent F420 reductase, at 1,183,273 to 1,183,944
_RS06390
MMJJ_RS06395: MMJJ_RS06395 - sugar phosphate isomerase/epimerase, at 1,183,966 to 1,184,772
_RS06395
MMJJ_RS06400: MMJJ_RS06400 - FumA C-terminus/TtdB family hydratase beta subunit, at 1,184,850 to 1,185,422
_RS06400
Position (kb)
1183
1184 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1182.293 kb on - strand, within MMJJ_RS06380 at 1182.311 kb on + strand, within MMJJ_RS06380 at 1182.359 kb on + strand, within MMJJ_RS06380 at 1182.359 kb on + strand, within MMJJ_RS06380 at 1182.461 kb on + strand, within MMJJ_RS06380 at 1182.491 kb on + strand, within MMJJ_RS06380 at 1182.908 kb on - strand at 1182.921 kb on + strand at 1182.932 kb on + strand at 1182.946 kb on - strand at 1182.967 kb on - strand at 1182.975 kb on + strand at 1182.975 kb on + strand at 1183.009 kb on + strand at 1183.013 kb on + strand at 1183.051 kb on - strand at 1183.063 kb on - strand at 1183.068 kb on - strand at 1183.083 kb on - strand, within MMJJ_RS06385 at 1183.084 kb on + strand, within MMJJ_RS06385 at 1183.129 kb on + strand, within MMJJ_RS06385 at 1183.135 kb on - strand, within MMJJ_RS06385 at 1183.135 kb on - strand, within MMJJ_RS06385 at 1183.137 kb on - strand, within MMJJ_RS06385 at 1183.137 kb on - strand, within MMJJ_RS06385 at 1183.138 kb on + strand, within MMJJ_RS06385 at 1183.138 kb on + strand, within MMJJ_RS06385 at 1183.138 kb on + strand, within MMJJ_RS06385 at 1183.182 kb on - strand, within MMJJ_RS06385 at 1183.186 kb on + strand, within MMJJ_RS06385 at 1183.186 kb on + strand, within MMJJ_RS06385 at 1183.188 kb on - strand, within MMJJ_RS06385 at 1183.199 kb on + strand, within MMJJ_RS06385 at 1183.264 kb on + strand at 1183.274 kb on - strand at 1183.291 kb on + strand at 1183.504 kb on - strand, within MMJJ_RS06390 at 1183.707 kb on - strand, within MMJJ_RS06390 at 1183.735 kb on - strand, within MMJJ_RS06390 at 1183.777 kb on + strand, within MMJJ_RS06390 at 1183.984 kb on - strand at 1184.027 kb on + strand at 1184.028 kb on - strand at 1184.028 kb on - strand at 1184.034 kb on - strand at 1184.034 kb on - strand at 1184.060 kb on - strand, within MMJJ_RS06395 at 1184.061 kb on + strand, within MMJJ_RS06395 at 1184.061 kb on + strand, within MMJJ_RS06395 at 1184.120 kb on + strand, within MMJJ_RS06395 at 1184.192 kb on + strand, within MMJJ_RS06395 at 1184.245 kb on + strand, within MMJJ_RS06395 at 1184.253 kb on - strand, within MMJJ_RS06395 at 1184.253 kb on - strand, within MMJJ_RS06395 at 1184.265 kb on + strand, within MMJJ_RS06395 at 1184.265 kb on + strand, within MMJJ_RS06395 at 1184.265 kb on + strand, within MMJJ_RS06395 at 1184.277 kb on - strand, within MMJJ_RS06395 at 1184.284 kb on - strand, within MMJJ_RS06395 at 1184.284 kb on - strand, within MMJJ_RS06395 at 1184.285 kb on + strand, within MMJJ_RS06395 at 1184.285 kb on + strand, within MMJJ_RS06395 at 1184.286 kb on - strand, within MMJJ_RS06395 at 1184.287 kb on + strand, within MMJJ_RS06395 at 1184.330 kb on - strand, within MMJJ_RS06395 at 1184.347 kb on - strand, within MMJJ_RS06395 at 1184.359 kb on - strand, within MMJJ_RS06395 at 1184.359 kb on - strand, within MMJJ_RS06395 at 1184.367 kb on + strand, within MMJJ_RS06395 at 1184.386 kb on - strand, within MMJJ_RS06395 at 1184.386 kb on - strand, within MMJJ_RS06395 at 1184.386 kb on - strand, within MMJJ_RS06395 at 1184.386 kb on - strand, within MMJJ_RS06395 at 1184.387 kb on + strand, within MMJJ_RS06395 at 1184.387 kb on + strand, within MMJJ_RS06395 at 1184.387 kb on + strand, within MMJJ_RS06395 at 1184.388 kb on - strand, within MMJJ_RS06395 at 1184.388 kb on - strand, within MMJJ_RS06395 at 1184.389 kb on + strand, within MMJJ_RS06395 at 1184.389 kb on + strand, within MMJJ_RS06395 at 1184.389 kb on + strand, within MMJJ_RS06395 at 1184.395 kb on - strand, within MMJJ_RS06395 at 1184.419 kb on - strand, within MMJJ_RS06395 at 1184.430 kb on - strand, within MMJJ_RS06395 at 1184.431 kb on + strand, within MMJJ_RS06395 at 1184.431 kb on + strand, within MMJJ_RS06395 at 1184.431 kb on + strand, within MMJJ_RS06395 at 1184.453 kb on + strand, within MMJJ_RS06395 at 1184.456 kb on - strand, within MMJJ_RS06395 at 1184.488 kb on - strand, within MMJJ_RS06395 at 1184.488 kb on - strand, within MMJJ_RS06395 at 1184.551 kb on + strand, within MMJJ_RS06395 at 1184.609 kb on - strand, within MMJJ_RS06395 at 1184.633 kb on - strand, within MMJJ_RS06395 at 1184.747 kb on + strand at 1184.765 kb on - strand at 1184.765 kb on - strand at 1184.776 kb on - strand at 1184.776 kb on - strand at 1184.791 kb on - strand at 1184.791 kb on - strand at 1184.792 kb on + strand at 1184.796 kb on + strand at 1184.796 kb on + strand at 1184.796 kb on + strand at 1184.798 kb on - strand at 1184.806 kb on - strand at 1184.806 kb on - strand at 1184.807 kb on + strand at 1184.817 kb on - strand at 1184.818 kb on + strand at 1184.821 kb on - strand at 1184.822 kb on + strand at 1184.928 kb on + strand, within MMJJ_RS06400
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 2g/L casaminos remove 1,182,293 - MMJJ_RS06380 0.54 -0.7 1,182,311 + MMJJ_RS06380 0.55 -1.3 1,182,359 + MMJJ_RS06380 0.59 -0.5 1,182,359 + MMJJ_RS06380 0.59 -1.5 1,182,461 + MMJJ_RS06380 0.66 +0.3 1,182,491 + MMJJ_RS06380 0.68 -3.1 1,182,908 - -0.1 1,182,921 + -2.4 1,182,932 + +1.3 1,182,946 - +0.7 1,182,967 - +1.1 1,182,975 + +1.1 1,182,975 + +0.7 1,183,009 + +1.1 1,183,013 + +1.3 1,183,051 - -0.0 1,183,063 - -0.0 1,183,068 - +0.8 1,183,083 - MMJJ_RS06385 0.13 +2.5 1,183,084 + MMJJ_RS06385 0.13 +1.2 1,183,129 + MMJJ_RS06385 0.33 -2.7 1,183,135 - MMJJ_RS06385 0.36 -0.0 1,183,135 - MMJJ_RS06385 0.36 +0.1 1,183,137 - MMJJ_RS06385 0.37 +0.5 1,183,137 - MMJJ_RS06385 0.37 +0.0 1,183,138 + MMJJ_RS06385 0.37 +1.5 1,183,138 + MMJJ_RS06385 0.37 +0.2 1,183,138 + MMJJ_RS06385 0.37 +0.6 1,183,182 - MMJJ_RS06385 0.57 +0.2 1,183,186 + MMJJ_RS06385 0.59 +0.2 1,183,186 + MMJJ_RS06385 0.59 +1.1 1,183,188 - MMJJ_RS06385 0.60 +0.8 1,183,199 + MMJJ_RS06385 0.65 +0.6 1,183,264 + -0.1 1,183,274 - +0.1 1,183,291 + +1.3 1,183,504 - MMJJ_RS06390 0.34 -3.7 1,183,707 - MMJJ_RS06390 0.65 -2.0 1,183,735 - MMJJ_RS06390 0.69 +0.7 1,183,777 + MMJJ_RS06390 0.75 -2.6 1,183,984 - +2.2 1,184,027 + -2.3 1,184,028 - +0.5 1,184,028 - -0.1 1,184,034 - +0.2 1,184,034 - +0.1 1,184,060 - MMJJ_RS06395 0.12 -0.1 1,184,061 + MMJJ_RS06395 0.12 -1.1 1,184,061 + MMJJ_RS06395 0.12 -0.7 1,184,120 + MMJJ_RS06395 0.19 -0.9 1,184,192 + MMJJ_RS06395 0.28 -1.3 1,184,245 + MMJJ_RS06395 0.35 -0.6 1,184,253 - MMJJ_RS06395 0.36 -0.4 1,184,253 - MMJJ_RS06395 0.36 -0.2 1,184,265 + MMJJ_RS06395 0.37 -0.8 1,184,265 + MMJJ_RS06395 0.37 -0.1 1,184,265 + MMJJ_RS06395 0.37 -0.2 1,184,277 - MMJJ_RS06395 0.39 -1.0 1,184,284 - MMJJ_RS06395 0.39 +0.4 1,184,284 - MMJJ_RS06395 0.39 -0.7 1,184,285 + MMJJ_RS06395 0.40 -1.0 1,184,285 + MMJJ_RS06395 0.40 -0.1 1,184,286 - MMJJ_RS06395 0.40 -0.0 1,184,287 + MMJJ_RS06395 0.40 -0.3 1,184,330 - MMJJ_RS06395 0.45 -1.4 1,184,347 - MMJJ_RS06395 0.47 +0.2 1,184,359 - MMJJ_RS06395 0.49 +0.4 1,184,359 - MMJJ_RS06395 0.49 +2.3 1,184,367 + MMJJ_RS06395 0.50 -2.5 1,184,386 - MMJJ_RS06395 0.52 -1.7 1,184,386 - MMJJ_RS06395 0.52 -0.7 1,184,386 - MMJJ_RS06395 0.52 -0.0 1,184,386 - MMJJ_RS06395 0.52 -1.7 1,184,387 + MMJJ_RS06395 0.52 +0.6 1,184,387 + MMJJ_RS06395 0.52 -1.0 1,184,387 + MMJJ_RS06395 0.52 +0.3 1,184,388 - MMJJ_RS06395 0.52 -0.4 1,184,388 - MMJJ_RS06395 0.52 -0.7 1,184,389 + MMJJ_RS06395 0.52 -0.5 1,184,389 + MMJJ_RS06395 0.52 -0.8 1,184,389 + MMJJ_RS06395 0.52 -0.8 1,184,395 - MMJJ_RS06395 0.53 +1.0 1,184,419 - MMJJ_RS06395 0.56 -0.6 1,184,430 - MMJJ_RS06395 0.57 -0.9 1,184,431 + MMJJ_RS06395 0.58 -0.6 1,184,431 + MMJJ_RS06395 0.58 -0.5 1,184,431 + MMJJ_RS06395 0.58 -0.2 1,184,453 + MMJJ_RS06395 0.60 -0.4 1,184,456 - MMJJ_RS06395 0.61 +0.5 1,184,488 - MMJJ_RS06395 0.65 +1.5 1,184,488 - MMJJ_RS06395 0.65 -0.7 1,184,551 + MMJJ_RS06395 0.72 +1.9 1,184,609 - MMJJ_RS06395 0.80 +0.3 1,184,633 - MMJJ_RS06395 0.83 -0.1 1,184,747 + -1.9 1,184,765 - +0.0 1,184,765 - -1.2 1,184,776 - -0.5 1,184,776 - -0.6 1,184,791 - -0.4 1,184,791 - +0.3 1,184,792 + -0.0 1,184,796 + -2.5 1,184,796 + -0.6 1,184,796 + -1.2 1,184,798 - +0.9 1,184,806 - -2.6 1,184,806 - -2.5 1,184,807 + -0.4 1,184,817 - -1.5 1,184,818 + +0.9 1,184,821 - -1.2 1,184,822 + +0.4 1,184,928 + MMJJ_RS06400 0.14 -0.9
Or see this region's nucleotide sequence