Experiment: H2 N-free with 2g/L casaminos
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS09320 and MMJJ_RS06370 are separated by 129 nucleotides MMJJ_RS06370 and MMJJ_RS06375 are separated by 159 nucleotides MMJJ_RS06375 and MMJJ_RS06380 are separated by 26 nucleotides MMJJ_RS06380 and MMJJ_RS06385 are separated by 137 nucleotides MMJJ_RS06385 and MMJJ_RS06390 overlap by 4 nucleotides
MMJJ_RS09320: MMJJ_RS09320 - tRNA-Met, at 1,180,493 to 1,180,607
_RS09320
MMJJ_RS06370: MMJJ_RS06370 - tRNA-Leu, at 1,180,737 to 1,180,821
_RS06370
MMJJ_RS06375: MMJJ_RS06375 - UbiX family flavin prenyltransferase, at 1,180,981 to 1,181,538
_RS06375
MMJJ_RS06380: MMJJ_RS06380 - signal recognition particle protein Srp54, at 1,181,565 to 1,182,917
_RS06380
MMJJ_RS06385: MMJJ_RS06385 - hypothetical protein, at 1,183,055 to 1,183,276
_RS06385
MMJJ_RS06390: MMJJ_RS06390 - NADPH-dependent F420 reductase, at 1,183,273 to 1,183,944
_RS06390
Position (kb)
1181
1182
1183 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1180.651 kb on + strand at 1180.858 kb on + strand at 1180.879 kb on - strand at 1180.880 kb on + strand at 1180.884 kb on - strand at 1180.884 kb on - strand at 1180.884 kb on - strand at 1180.884 kb on - strand at 1180.884 kb on - strand at 1180.884 kb on - strand at 1180.884 kb on - strand at 1180.884 kb on - strand at 1180.884 kb on - strand at 1180.884 kb on - strand at 1180.884 kb on - strand at 1180.884 kb on - strand at 1180.884 kb on - strand at 1180.885 kb on + strand at 1180.885 kb on + strand at 1180.885 kb on + strand at 1180.885 kb on + strand at 1180.885 kb on + strand at 1180.885 kb on + strand at 1180.885 kb on + strand at 1180.885 kb on + strand at 1180.885 kb on + strand at 1180.889 kb on - strand at 1180.889 kb on - strand at 1180.896 kb on + strand at 1180.930 kb on - strand at 1180.930 kb on - strand at 1180.930 kb on - strand at 1180.930 kb on - strand at 1180.930 kb on - strand at 1180.930 kb on - strand at 1180.930 kb on - strand at 1180.930 kb on - strand at 1180.930 kb on - strand at 1180.930 kb on - strand at 1180.930 kb on - strand at 1180.930 kb on - strand at 1180.930 kb on - strand at 1180.931 kb on + strand at 1180.931 kb on + strand at 1180.931 kb on + strand at 1180.931 kb on + strand at 1180.931 kb on + strand at 1180.931 kb on + strand at 1180.931 kb on + strand at 1180.931 kb on + strand at 1180.931 kb on + strand at 1180.931 kb on + strand at 1180.932 kb on - strand at 1180.932 kb on - strand at 1180.932 kb on - strand at 1180.933 kb on + strand at 1180.933 kb on + strand at 1180.933 kb on + strand at 1180.934 kb on - strand at 1180.934 kb on - strand at 1180.934 kb on - strand at 1180.935 kb on + strand at 1180.936 kb on - strand at 1180.937 kb on + strand at 1180.937 kb on + strand at 1180.937 kb on + strand at 1180.958 kb on + strand at 1180.961 kb on + strand at 1180.961 kb on + strand at 1180.961 kb on + strand at 1180.963 kb on + strand at 1180.980 kb on - strand at 1180.995 kb on + strand at 1180.995 kb on + strand at 1181.272 kb on - strand, within MMJJ_RS06375 at 1181.280 kb on - strand, within MMJJ_RS06375 at 1181.331 kb on + strand, within MMJJ_RS06375 at 1181.359 kb on + strand, within MMJJ_RS06375 at 1181.361 kb on - strand, within MMJJ_RS06375 at 1181.390 kb on - strand, within MMJJ_RS06375 at 1181.435 kb on + strand, within MMJJ_RS06375 at 1181.453 kb on - strand, within MMJJ_RS06375 at 1181.453 kb on - strand, within MMJJ_RS06375 at 1181.517 kb on + strand at 1181.520 kb on - strand at 1181.536 kb on - strand at 1181.536 kb on - strand at 1181.537 kb on + strand at 1181.537 kb on + strand at 1181.547 kb on - strand at 1181.550 kb on - strand at 1181.564 kb on - strand at 1181.876 kb on + strand, within MMJJ_RS06380 at 1181.934 kb on + strand, within MMJJ_RS06380 at 1181.995 kb on + strand, within MMJJ_RS06380 at 1182.005 kb on + strand, within MMJJ_RS06380 at 1182.218 kb on + strand, within MMJJ_RS06380 at 1182.293 kb on - strand, within MMJJ_RS06380 at 1182.311 kb on + strand, within MMJJ_RS06380 at 1182.359 kb on + strand, within MMJJ_RS06380 at 1182.359 kb on + strand, within MMJJ_RS06380 at 1182.461 kb on + strand, within MMJJ_RS06380 at 1182.491 kb on + strand, within MMJJ_RS06380 at 1182.908 kb on - strand at 1182.921 kb on + strand at 1182.932 kb on + strand at 1182.946 kb on - strand at 1182.967 kb on - strand at 1182.975 kb on + strand at 1182.975 kb on + strand at 1183.009 kb on + strand at 1183.013 kb on + strand at 1183.051 kb on - strand at 1183.063 kb on - strand at 1183.068 kb on - strand at 1183.083 kb on - strand, within MMJJ_RS06385 at 1183.084 kb on + strand, within MMJJ_RS06385 at 1183.129 kb on + strand, within MMJJ_RS06385 at 1183.135 kb on - strand, within MMJJ_RS06385 at 1183.135 kb on - strand, within MMJJ_RS06385 at 1183.137 kb on - strand, within MMJJ_RS06385 at 1183.137 kb on - strand, within MMJJ_RS06385 at 1183.138 kb on + strand, within MMJJ_RS06385 at 1183.138 kb on + strand, within MMJJ_RS06385 at 1183.138 kb on + strand, within MMJJ_RS06385 at 1183.182 kb on - strand, within MMJJ_RS06385 at 1183.186 kb on + strand, within MMJJ_RS06385 at 1183.186 kb on + strand, within MMJJ_RS06385 at 1183.188 kb on - strand, within MMJJ_RS06385 at 1183.199 kb on + strand, within MMJJ_RS06385 at 1183.264 kb on + strand at 1183.274 kb on - strand at 1183.291 kb on + strand at 1183.504 kb on - strand, within MMJJ_RS06390 at 1183.707 kb on - strand, within MMJJ_RS06390 at 1183.735 kb on - strand, within MMJJ_RS06390 at 1183.777 kb on + strand, within MMJJ_RS06390
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 2g/L casaminos remove 1,180,651 + -0.3 1,180,858 + +1.2 1,180,879 - +0.6 1,180,880 + -1.1 1,180,884 - +0.8 1,180,884 - -0.5 1,180,884 - -0.4 1,180,884 - -0.0 1,180,884 - +0.2 1,180,884 - +0.5 1,180,884 - +0.4 1,180,884 - +0.2 1,180,884 - +1.2 1,180,884 - +1.4 1,180,884 - +0.7 1,180,884 - -0.2 1,180,884 - +0.5 1,180,885 + -0.8 1,180,885 + -0.2 1,180,885 + +1.1 1,180,885 + -0.1 1,180,885 + +1.8 1,180,885 + +1.1 1,180,885 + +1.3 1,180,885 + -0.5 1,180,885 + -1.5 1,180,889 - +0.9 1,180,889 - +1.2 1,180,896 + +0.4 1,180,930 - +0.1 1,180,930 - +0.9 1,180,930 - +0.2 1,180,930 - +0.5 1,180,930 - +1.7 1,180,930 - -0.3 1,180,930 - -0.4 1,180,930 - -0.2 1,180,930 - +2.3 1,180,930 - +1.4 1,180,930 - +1.5 1,180,930 - -0.2 1,180,930 - +1.4 1,180,931 + -0.3 1,180,931 + +0.6 1,180,931 + -0.6 1,180,931 + +0.8 1,180,931 + -1.6 1,180,931 + -1.3 1,180,931 + +0.8 1,180,931 + +0.1 1,180,931 + -0.4 1,180,931 + +1.3 1,180,932 - -1.5 1,180,932 - -0.2 1,180,932 - -1.3 1,180,933 + -0.0 1,180,933 + +0.7 1,180,933 + +0.2 1,180,934 - -0.2 1,180,934 - +1.4 1,180,934 - +0.4 1,180,935 + +1.3 1,180,936 - -0.5 1,180,937 + +0.3 1,180,937 + +0.6 1,180,937 + +2.0 1,180,958 + +0.9 1,180,961 + -0.2 1,180,961 + +0.6 1,180,961 + -0.1 1,180,963 + +0.5 1,180,980 - -1.3 1,180,995 + -3.0 1,180,995 + -2.5 1,181,272 - MMJJ_RS06375 0.52 -1.7 1,181,280 - MMJJ_RS06375 0.54 -3.5 1,181,331 + MMJJ_RS06375 0.63 -2.6 1,181,359 + MMJJ_RS06375 0.68 -2.0 1,181,361 - MMJJ_RS06375 0.68 -0.4 1,181,390 - MMJJ_RS06375 0.73 +0.8 1,181,435 + MMJJ_RS06375 0.81 -3.4 1,181,453 - MMJJ_RS06375 0.85 -1.2 1,181,453 - MMJJ_RS06375 0.85 -2.9 1,181,517 + -1.5 1,181,520 - +0.3 1,181,536 - +1.2 1,181,536 - -3.3 1,181,537 + -1.1 1,181,537 + +1.8 1,181,547 - -3.9 1,181,550 - -0.9 1,181,564 - +0.7 1,181,876 + MMJJ_RS06380 0.23 -1.9 1,181,934 + MMJJ_RS06380 0.27 -2.1 1,181,995 + MMJJ_RS06380 0.32 -2.1 1,182,005 + MMJJ_RS06380 0.33 -1.3 1,182,218 + MMJJ_RS06380 0.48 -0.7 1,182,293 - MMJJ_RS06380 0.54 -0.7 1,182,311 + MMJJ_RS06380 0.55 -1.3 1,182,359 + MMJJ_RS06380 0.59 -0.5 1,182,359 + MMJJ_RS06380 0.59 -1.5 1,182,461 + MMJJ_RS06380 0.66 +0.3 1,182,491 + MMJJ_RS06380 0.68 -3.1 1,182,908 - -0.1 1,182,921 + -2.4 1,182,932 + +1.3 1,182,946 - +0.7 1,182,967 - +1.1 1,182,975 + +1.1 1,182,975 + +0.7 1,183,009 + +1.1 1,183,013 + +1.3 1,183,051 - -0.0 1,183,063 - -0.0 1,183,068 - +0.8 1,183,083 - MMJJ_RS06385 0.13 +2.5 1,183,084 + MMJJ_RS06385 0.13 +1.2 1,183,129 + MMJJ_RS06385 0.33 -2.7 1,183,135 - MMJJ_RS06385 0.36 -0.0 1,183,135 - MMJJ_RS06385 0.36 +0.1 1,183,137 - MMJJ_RS06385 0.37 +0.5 1,183,137 - MMJJ_RS06385 0.37 +0.0 1,183,138 + MMJJ_RS06385 0.37 +1.5 1,183,138 + MMJJ_RS06385 0.37 +0.2 1,183,138 + MMJJ_RS06385 0.37 +0.6 1,183,182 - MMJJ_RS06385 0.57 +0.2 1,183,186 + MMJJ_RS06385 0.59 +0.2 1,183,186 + MMJJ_RS06385 0.59 +1.1 1,183,188 - MMJJ_RS06385 0.60 +0.8 1,183,199 + MMJJ_RS06385 0.65 +0.6 1,183,264 + -0.1 1,183,274 - +0.1 1,183,291 + +1.3 1,183,504 - MMJJ_RS06390 0.34 -3.7 1,183,707 - MMJJ_RS06390 0.65 -2.0 1,183,735 - MMJJ_RS06390 0.69 +0.7 1,183,777 + MMJJ_RS06390 0.75 -2.6
Or see this region's nucleotide sequence