Experiment: H2 N-free with 2g/L casaminos
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS06200 and MMJJ_RS06205 are separated by 32 nucleotides MMJJ_RS06205 and MMJJ_RS06210 are separated by 22 nucleotides MMJJ_RS06210 and MMJJ_RS06215 are separated by 102 nucleotides MMJJ_RS06215 and MMJJ_RS06220 are separated by 36 nucleotides MMJJ_RS06220 and MMJJ_RS06225 overlap by 4 nucleotides
MMJJ_RS06200: MMJJ_RS06200 - phosphoglycerate dehydrogenase, at 1,150,473 to 1,152,044
_RS06200
MMJJ_RS06205: MMJJ_RS06205 - EamA family transporter, at 1,152,077 to 1,152,490
_RS06205
MMJJ_RS06210: MMJJ_RS06210 - hypothetical protein, at 1,152,513 to 1,152,755
_RS06210
MMJJ_RS06215: MMJJ_RS06215 - agmatinase, at 1,152,858 to 1,153,706
_RS06215
MMJJ_RS06220: MMJJ_RS06220 - polyamine aminopropyltransferase, at 1,153,743 to 1,154,585
_RS06220
MMJJ_RS06225: MMJJ_RS06225 - adenosylmethionine decarboxylase, at 1,154,582 to 1,154,950
_RS06225
Position (kb)
1152
1153
1154 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1151.957 kb on + strand at 1151.986 kb on - strand at 1152.017 kb on + strand at 1152.042 kb on - strand at 1152.042 kb on - strand at 1152.043 kb on + strand at 1152.043 kb on + strand at 1152.088 kb on + strand at 1152.088 kb on + strand at 1152.099 kb on - strand at 1152.099 kb on - strand at 1152.100 kb on + strand at 1152.100 kb on + strand at 1152.100 kb on + strand at 1152.114 kb on - strand at 1152.115 kb on + strand at 1152.115 kb on + strand at 1152.115 kb on + strand at 1152.116 kb on - strand at 1152.116 kb on - strand at 1152.117 kb on + strand at 1152.121 kb on - strand, within MMJJ_RS06205 at 1152.122 kb on + strand, within MMJJ_RS06205 at 1152.122 kb on + strand, within MMJJ_RS06205 at 1152.123 kb on - strand, within MMJJ_RS06205 at 1152.123 kb on - strand, within MMJJ_RS06205 at 1152.123 kb on - strand, within MMJJ_RS06205 at 1152.123 kb on - strand, within MMJJ_RS06205 at 1152.124 kb on + strand, within MMJJ_RS06205 at 1152.124 kb on + strand, within MMJJ_RS06205 at 1152.124 kb on + strand, within MMJJ_RS06205 at 1152.124 kb on + strand, within MMJJ_RS06205 at 1152.124 kb on + strand, within MMJJ_RS06205 at 1152.124 kb on + strand, within MMJJ_RS06205 at 1152.127 kb on - strand, within MMJJ_RS06205 at 1152.183 kb on - strand, within MMJJ_RS06205 at 1152.184 kb on + strand, within MMJJ_RS06205 at 1152.187 kb on - strand, within MMJJ_RS06205 at 1152.204 kb on - strand, within MMJJ_RS06205 at 1152.204 kb on - strand, within MMJJ_RS06205 at 1152.205 kb on + strand, within MMJJ_RS06205 at 1152.205 kb on + strand, within MMJJ_RS06205 at 1152.205 kb on + strand, within MMJJ_RS06205 at 1152.205 kb on + strand, within MMJJ_RS06205 at 1152.205 kb on + strand, within MMJJ_RS06205 at 1152.211 kb on - strand, within MMJJ_RS06205 at 1152.211 kb on - strand, within MMJJ_RS06205 at 1152.211 kb on - strand, within MMJJ_RS06205 at 1152.211 kb on - strand, within MMJJ_RS06205 at 1152.212 kb on + strand, within MMJJ_RS06205 at 1152.213 kb on - strand, within MMJJ_RS06205 at 1152.213 kb on - strand, within MMJJ_RS06205 at 1152.216 kb on - strand, within MMJJ_RS06205 at 1152.216 kb on - strand, within MMJJ_RS06205 at 1152.216 kb on - strand, within MMJJ_RS06205 at 1152.217 kb on + strand, within MMJJ_RS06205 at 1152.297 kb on - strand, within MMJJ_RS06205 at 1152.298 kb on + strand, within MMJJ_RS06205 at 1152.352 kb on + strand, within MMJJ_RS06205 at 1152.352 kb on + strand, within MMJJ_RS06205 at 1152.360 kb on - strand, within MMJJ_RS06205 at 1152.454 kb on + strand at 1152.454 kb on + strand at 1152.456 kb on - strand at 1152.466 kb on + strand at 1152.466 kb on + strand at 1152.471 kb on - strand at 1152.482 kb on + strand at 1152.555 kb on - strand, within MMJJ_RS06210 at 1152.609 kb on - strand, within MMJJ_RS06210 at 1152.620 kb on - strand, within MMJJ_RS06210 at 1152.684 kb on - strand, within MMJJ_RS06210 at 1152.684 kb on - strand, within MMJJ_RS06210 at 1152.684 kb on - strand, within MMJJ_RS06210 at 1152.685 kb on + strand, within MMJJ_RS06210 at 1152.685 kb on + strand, within MMJJ_RS06210 at 1152.688 kb on - strand, within MMJJ_RS06210 at 1152.688 kb on - strand, within MMJJ_RS06210 at 1152.688 kb on - strand, within MMJJ_RS06210 at 1152.689 kb on + strand, within MMJJ_RS06210 at 1152.689 kb on + strand, within MMJJ_RS06210 at 1152.689 kb on + strand, within MMJJ_RS06210 at 1152.692 kb on - strand, within MMJJ_RS06210 at 1152.693 kb on + strand, within MMJJ_RS06210 at 1152.693 kb on + strand, within MMJJ_RS06210 at 1152.717 kb on - strand, within MMJJ_RS06210 at 1152.729 kb on + strand at 1152.739 kb on + strand at 1152.774 kb on + strand at 1152.786 kb on - strand at 1152.786 kb on - strand at 1152.786 kb on - strand at 1152.786 kb on - strand at 1152.786 kb on - strand at 1152.787 kb on + strand at 1152.787 kb on + strand at 1152.787 kb on + strand at 1152.787 kb on + strand at 1152.793 kb on + strand at 1152.805 kb on + strand at 1152.826 kb on - strand at 1152.826 kb on - strand at 1152.859 kb on - strand at 1152.872 kb on - strand at 1152.877 kb on + strand at 1152.914 kb on - strand at 1152.915 kb on + strand at 1152.915 kb on + strand at 1153.008 kb on + strand, within MMJJ_RS06215 at 1153.074 kb on - strand, within MMJJ_RS06215 at 1153.075 kb on + strand, within MMJJ_RS06215 at 1153.075 kb on + strand, within MMJJ_RS06215 at 1153.161 kb on + strand, within MMJJ_RS06215 at 1153.227 kb on + strand, within MMJJ_RS06215 at 1153.257 kb on - strand, within MMJJ_RS06215 at 1153.288 kb on - strand, within MMJJ_RS06215 at 1153.288 kb on - strand, within MMJJ_RS06215 at 1153.289 kb on + strand, within MMJJ_RS06215 at 1153.289 kb on + strand, within MMJJ_RS06215 at 1153.493 kb on + strand, within MMJJ_RS06215 at 1153.509 kb on - strand, within MMJJ_RS06215 at 1153.541 kb on - strand, within MMJJ_RS06215 at 1153.670 kb on + strand at 1153.670 kb on + strand at 1153.670 kb on + strand at 1153.670 kb on + strand at 1153.670 kb on + strand at 1153.728 kb on - strand at 1153.745 kb on + strand at 1153.745 kb on + strand at 1153.752 kb on - strand at 1153.752 kb on - strand at 1153.752 kb on - strand at 1153.752 kb on - strand at 1153.752 kb on - strand at 1153.753 kb on + strand at 1153.757 kb on + strand at 1153.757 kb on + strand at 1153.760 kb on - strand at 1153.760 kb on - strand at 1153.761 kb on + strand at 1153.776 kb on - strand at 1153.805 kb on + strand at 1153.805 kb on + strand at 1153.886 kb on - strand, within MMJJ_RS06220 at 1153.909 kb on - strand, within MMJJ_RS06220 at 1153.910 kb on + strand, within MMJJ_RS06220 at 1153.922 kb on - strand, within MMJJ_RS06220 at 1153.923 kb on + strand, within MMJJ_RS06220 at 1153.927 kb on + strand, within MMJJ_RS06220 at 1153.927 kb on + strand, within MMJJ_RS06220 at 1153.983 kb on - strand, within MMJJ_RS06220 at 1153.999 kb on + strand, within MMJJ_RS06220 at 1154.054 kb on + strand, within MMJJ_RS06220 at 1154.245 kb on + strand, within MMJJ_RS06220 at 1154.291 kb on - strand, within MMJJ_RS06220 at 1154.292 kb on + strand, within MMJJ_RS06220 at 1154.401 kb on + strand, within MMJJ_RS06220 at 1154.402 kb on - strand, within MMJJ_RS06220 at 1154.515 kb on + strand at 1154.515 kb on + strand at 1154.608 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 2g/L casaminos remove 1,151,957 + +0.3 1,151,986 - -0.4 1,152,017 + +0.9 1,152,042 - +0.2 1,152,042 - +0.5 1,152,043 + -0.6 1,152,043 + +0.3 1,152,088 + +0.5 1,152,088 + +0.8 1,152,099 - +0.3 1,152,099 - +1.0 1,152,100 + +0.8 1,152,100 + -1.9 1,152,100 + +1.3 1,152,114 - -1.0 1,152,115 + -0.2 1,152,115 + +0.5 1,152,115 + +1.6 1,152,116 - +0.5 1,152,116 - +0.8 1,152,117 + +0.4 1,152,121 - MMJJ_RS06205 0.11 +0.7 1,152,122 + MMJJ_RS06205 0.11 -0.0 1,152,122 + MMJJ_RS06205 0.11 -1.0 1,152,123 - MMJJ_RS06205 0.11 +0.9 1,152,123 - MMJJ_RS06205 0.11 -0.5 1,152,123 - MMJJ_RS06205 0.11 +0.2 1,152,123 - MMJJ_RS06205 0.11 +0.9 1,152,124 + MMJJ_RS06205 0.11 +0.6 1,152,124 + MMJJ_RS06205 0.11 +1.2 1,152,124 + MMJJ_RS06205 0.11 +0.2 1,152,124 + MMJJ_RS06205 0.11 -0.4 1,152,124 + MMJJ_RS06205 0.11 -0.2 1,152,124 + MMJJ_RS06205 0.11 +1.5 1,152,127 - MMJJ_RS06205 0.12 +0.1 1,152,183 - MMJJ_RS06205 0.26 -1.7 1,152,184 + MMJJ_RS06205 0.26 +1.5 1,152,187 - MMJJ_RS06205 0.27 -1.0 1,152,204 - MMJJ_RS06205 0.31 +0.9 1,152,204 - MMJJ_RS06205 0.31 +1.3 1,152,205 + MMJJ_RS06205 0.31 +0.2 1,152,205 + MMJJ_RS06205 0.31 +1.2 1,152,205 + MMJJ_RS06205 0.31 +0.6 1,152,205 + MMJJ_RS06205 0.31 +0.3 1,152,205 + MMJJ_RS06205 0.31 +0.4 1,152,211 - MMJJ_RS06205 0.32 +1.0 1,152,211 - MMJJ_RS06205 0.32 +0.7 1,152,211 - MMJJ_RS06205 0.32 +0.9 1,152,211 - MMJJ_RS06205 0.32 +1.4 1,152,212 + MMJJ_RS06205 0.33 +1.1 1,152,213 - MMJJ_RS06205 0.33 +2.4 1,152,213 - MMJJ_RS06205 0.33 -1.6 1,152,216 - MMJJ_RS06205 0.34 -0.1 1,152,216 - MMJJ_RS06205 0.34 +0.3 1,152,216 - MMJJ_RS06205 0.34 -0.7 1,152,217 + MMJJ_RS06205 0.34 +0.7 1,152,297 - MMJJ_RS06205 0.53 +0.1 1,152,298 + MMJJ_RS06205 0.53 +1.1 1,152,352 + MMJJ_RS06205 0.66 +0.8 1,152,352 + MMJJ_RS06205 0.66 -0.2 1,152,360 - MMJJ_RS06205 0.68 +0.4 1,152,454 + +0.3 1,152,454 + +1.1 1,152,456 - +1.6 1,152,466 + -0.2 1,152,466 + +0.9 1,152,471 - +1.0 1,152,482 + +1.1 1,152,555 - MMJJ_RS06210 0.17 -0.9 1,152,609 - MMJJ_RS06210 0.40 +0.7 1,152,620 - MMJJ_RS06210 0.44 -1.0 1,152,684 - MMJJ_RS06210 0.70 +0.6 1,152,684 - MMJJ_RS06210 0.70 +0.7 1,152,684 - MMJJ_RS06210 0.70 -0.2 1,152,685 + MMJJ_RS06210 0.71 +0.6 1,152,685 + MMJJ_RS06210 0.71 -0.1 1,152,688 - MMJJ_RS06210 0.72 -1.7 1,152,688 - MMJJ_RS06210 0.72 -3.3 1,152,688 - MMJJ_RS06210 0.72 -1.4 1,152,689 + MMJJ_RS06210 0.72 -2.1 1,152,689 + MMJJ_RS06210 0.72 -2.1 1,152,689 + MMJJ_RS06210 0.72 -0.0 1,152,692 - MMJJ_RS06210 0.74 -0.2 1,152,693 + MMJJ_RS06210 0.74 -0.8 1,152,693 + MMJJ_RS06210 0.74 -0.8 1,152,717 - MMJJ_RS06210 0.84 -0.2 1,152,729 + -1.0 1,152,739 + -0.4 1,152,774 + -0.0 1,152,786 - -1.1 1,152,786 - -2.0 1,152,786 - +0.4 1,152,786 - -2.5 1,152,786 - +0.4 1,152,787 + +0.4 1,152,787 + -1.2 1,152,787 + +0.7 1,152,787 + -1.2 1,152,793 + -0.7 1,152,805 + +0.1 1,152,826 - +0.7 1,152,826 - +1.3 1,152,859 - +0.5 1,152,872 - -1.3 1,152,877 + -3.7 1,152,914 - -1.0 1,152,915 + -1.2 1,152,915 + -1.5 1,153,008 + MMJJ_RS06215 0.18 -1.8 1,153,074 - MMJJ_RS06215 0.25 -4.6 1,153,075 + MMJJ_RS06215 0.26 -1.9 1,153,075 + MMJJ_RS06215 0.26 -2.7 1,153,161 + MMJJ_RS06215 0.36 -1.7 1,153,227 + MMJJ_RS06215 0.43 -1.0 1,153,257 - MMJJ_RS06215 0.47 -2.2 1,153,288 - MMJJ_RS06215 0.51 -0.6 1,153,288 - MMJJ_RS06215 0.51 -1.4 1,153,289 + MMJJ_RS06215 0.51 -1.9 1,153,289 + MMJJ_RS06215 0.51 -2.4 1,153,493 + MMJJ_RS06215 0.75 -2.5 1,153,509 - MMJJ_RS06215 0.77 -3.7 1,153,541 - MMJJ_RS06215 0.80 -1.9 1,153,670 + -2.8 1,153,670 + -2.7 1,153,670 + -1.2 1,153,670 + -2.3 1,153,670 + -1.9 1,153,728 - +1.2 1,153,745 + +0.7 1,153,745 + -1.6 1,153,752 - +1.4 1,153,752 - -0.5 1,153,752 - -1.3 1,153,752 - -1.1 1,153,752 - -0.8 1,153,753 + -0.1 1,153,757 + -1.5 1,153,757 + -1.9 1,153,760 - -1.6 1,153,760 - -1.3 1,153,761 + -1.4 1,153,776 - -3.0 1,153,805 + -2.4 1,153,805 + -2.8 1,153,886 - MMJJ_RS06220 0.17 -2.4 1,153,909 - MMJJ_RS06220 0.20 -3.2 1,153,910 + MMJJ_RS06220 0.20 -0.6 1,153,922 - MMJJ_RS06220 0.21 -2.8 1,153,923 + MMJJ_RS06220 0.21 -1.1 1,153,927 + MMJJ_RS06220 0.22 -0.7 1,153,927 + MMJJ_RS06220 0.22 -4.5 1,153,983 - MMJJ_RS06220 0.28 -0.5 1,153,999 + MMJJ_RS06220 0.30 -2.9 1,154,054 + MMJJ_RS06220 0.37 -1.7 1,154,245 + MMJJ_RS06220 0.60 -1.1 1,154,291 - MMJJ_RS06220 0.65 -2.2 1,154,292 + MMJJ_RS06220 0.65 -3.5 1,154,401 + MMJJ_RS06220 0.78 -2.1 1,154,402 - MMJJ_RS06220 0.78 -2.7 1,154,515 + -2.1 1,154,515 + -3.3 1,154,608 - -1.9
Or see this region's nucleotide sequence