Experiment: H2 N-free with 2g/L casaminos
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS05985 and MMJJ_RS05990 are separated by 161 nucleotides MMJJ_RS05990 and MMJJ_RS05995 are separated by 2 nucleotides MMJJ_RS05995 and MMJJ_RS06000 are separated by 152 nucleotides MMJJ_RS06000 and MMJJ_RS06005 are separated by 25 nucleotides
MMJJ_RS05985: MMJJ_RS05985 - ABC transporter substrate-binding protein, at 1,113,051 to 1,113,773
_RS05985
MMJJ_RS05990: MMJJ_RS05990 - phosphatase PAP2 family protein, at 1,113,935 to 1,114,483
_RS05990
MMJJ_RS05995: MMJJ_RS05995 - ATP-binding cassette domain-containing protein, at 1,114,486 to 1,116,135
_RS05995
MMJJ_RS06000: MMJJ_RS06000 - cation:proton antiporter subunit C, at 1,116,288 to 1,116,644
_RS06000
MMJJ_RS06005: MMJJ_RS06005 - energy conserving hydrogenase EhbF, at 1,116,670 to 1,118,118
_RS06005
Position (kb)
1114
1115
1116
1117 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1113.556 kb on - strand, within MMJJ_RS05985 at 1113.656 kb on + strand, within MMJJ_RS05985 at 1113.695 kb on - strand, within MMJJ_RS05985 at 1113.696 kb on + strand, within MMJJ_RS05985 at 1113.696 kb on + strand, within MMJJ_RS05985 at 1113.704 kb on - strand at 1113.704 kb on - strand at 1113.705 kb on + strand at 1113.719 kb on - strand at 1113.720 kb on + strand at 1113.720 kb on + strand at 1113.720 kb on + strand at 1113.738 kb on - strand at 1113.756 kb on - strand at 1113.823 kb on - strand at 1113.824 kb on + strand at 1113.825 kb on - strand at 1113.825 kb on - strand at 1113.825 kb on - strand at 1113.825 kb on - strand at 1113.825 kb on - strand at 1113.825 kb on - strand at 1113.825 kb on - strand at 1113.826 kb on + strand at 1113.826 kb on + strand at 1113.826 kb on + strand at 1113.826 kb on + strand at 1113.826 kb on + strand at 1113.826 kb on + strand at 1113.826 kb on + strand at 1113.826 kb on + strand at 1113.826 kb on + strand at 1113.826 kb on + strand at 1113.836 kb on - strand at 1113.836 kb on - strand at 1113.837 kb on + strand at 1113.843 kb on - strand at 1113.844 kb on + strand at 1113.844 kb on + strand at 1113.857 kb on - strand at 1113.868 kb on - strand at 1113.869 kb on + strand at 1113.870 kb on - strand at 1113.870 kb on - strand at 1113.887 kb on - strand at 1113.887 kb on - strand at 1113.888 kb on + strand at 1113.888 kb on + strand at 1113.892 kb on + strand at 1113.933 kb on - strand at 1113.940 kb on + strand at 1113.998 kb on + strand, within MMJJ_RS05990 at 1113.998 kb on + strand, within MMJJ_RS05990 at 1114.044 kb on - strand, within MMJJ_RS05990 at 1114.045 kb on + strand, within MMJJ_RS05990 at 1114.045 kb on + strand, within MMJJ_RS05990 at 1114.045 kb on + strand, within MMJJ_RS05990 at 1114.046 kb on - strand, within MMJJ_RS05990 at 1114.046 kb on - strand, within MMJJ_RS05990 at 1114.046 kb on - strand, within MMJJ_RS05990 at 1114.046 kb on - strand, within MMJJ_RS05990 at 1114.046 kb on - strand, within MMJJ_RS05990 at 1114.046 kb on - strand, within MMJJ_RS05990 at 1114.046 kb on - strand, within MMJJ_RS05990 at 1114.046 kb on - strand, within MMJJ_RS05990 at 1114.047 kb on + strand, within MMJJ_RS05990 at 1114.113 kb on - strand, within MMJJ_RS05990 at 1114.132 kb on + strand, within MMJJ_RS05990 at 1114.140 kb on - strand, within MMJJ_RS05990 at 1114.140 kb on - strand, within MMJJ_RS05990 at 1114.140 kb on - strand, within MMJJ_RS05990 at 1114.141 kb on + strand, within MMJJ_RS05990 at 1114.141 kb on + strand, within MMJJ_RS05990 at 1114.141 kb on + strand, within MMJJ_RS05990 at 1114.143 kb on - strand, within MMJJ_RS05990 at 1114.152 kb on - strand, within MMJJ_RS05990 at 1114.164 kb on - strand, within MMJJ_RS05990 at 1114.216 kb on - strand, within MMJJ_RS05990 at 1114.350 kb on - strand, within MMJJ_RS05990 at 1114.434 kb on - strand at 1114.435 kb on + strand at 1114.435 kb on + strand at 1114.438 kb on + strand at 1114.447 kb on - strand at 1114.456 kb on - strand at 1114.477 kb on - strand at 1114.482 kb on + strand at 1114.482 kb on + strand at 1114.482 kb on + strand at 1114.498 kb on + strand at 1114.580 kb on + strand at 1115.372 kb on + strand, within MMJJ_RS05995 at 1115.444 kb on + strand, within MMJJ_RS05995 at 1115.638 kb on - strand, within MMJJ_RS05995 at 1115.639 kb on + strand, within MMJJ_RS05995 at 1115.870 kb on - strand, within MMJJ_RS05995 at 1116.046 kb on - strand at 1116.049 kb on + strand at 1116.049 kb on + strand at 1116.161 kb on - strand at 1116.168 kb on + strand at 1116.168 kb on + strand at 1116.178 kb on + strand at 1116.178 kb on + strand at 1116.179 kb on - strand at 1116.179 kb on - strand at 1116.180 kb on + strand at 1116.181 kb on - strand at 1116.181 kb on - strand at 1116.181 kb on - strand at 1116.181 kb on - strand at 1116.181 kb on - strand at 1116.181 kb on - strand at 1116.182 kb on + strand at 1116.183 kb on - strand at 1116.183 kb on - strand at 1116.184 kb on + strand at 1116.184 kb on + strand at 1116.184 kb on + strand at 1116.184 kb on + strand at 1116.184 kb on + strand at 1116.184 kb on + strand at 1116.184 kb on + strand at 1116.196 kb on + strand at 1116.201 kb on - strand at 1116.201 kb on - strand at 1116.206 kb on + strand at 1116.206 kb on + strand at 1116.211 kb on - strand at 1116.213 kb on - strand at 1116.284 kb on - strand at 1116.313 kb on - strand at 1116.313 kb on - strand at 1116.314 kb on + strand at 1116.329 kb on + strand, within MMJJ_RS06000 at 1116.345 kb on - strand, within MMJJ_RS06000 at 1116.346 kb on + strand, within MMJJ_RS06000 at 1116.438 kb on - strand, within MMJJ_RS06000 at 1116.462 kb on + strand, within MMJJ_RS06000 at 1116.534 kb on + strand, within MMJJ_RS06000 at 1116.587 kb on + strand, within MMJJ_RS06000 at 1116.587 kb on + strand, within MMJJ_RS06000 at 1116.677 kb on - strand at 1116.677 kb on - strand at 1116.702 kb on - strand at 1116.703 kb on + strand at 1116.745 kb on + strand at 1116.768 kb on - strand at 1116.768 kb on - strand at 1116.768 kb on - strand at 1116.769 kb on + strand at 1116.769 kb on + strand at 1116.769 kb on + strand at 1116.782 kb on - strand at 1116.782 kb on - strand at 1116.820 kb on - strand, within MMJJ_RS06005 at 1116.878 kb on + strand, within MMJJ_RS06005 at 1116.898 kb on + strand, within MMJJ_RS06005 at 1117.045 kb on - strand, within MMJJ_RS06005 at 1117.046 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 2g/L casaminos remove 1,113,556 - MMJJ_RS05985 0.70 +0.1 1,113,656 + MMJJ_RS05985 0.84 +0.5 1,113,695 - MMJJ_RS05985 0.89 +0.6 1,113,696 + MMJJ_RS05985 0.89 -1.8 1,113,696 + MMJJ_RS05985 0.89 -0.2 1,113,704 - +0.5 1,113,704 - +0.6 1,113,705 + +0.0 1,113,719 - -0.7 1,113,720 + +0.2 1,113,720 + -1.2 1,113,720 + +0.2 1,113,738 - +1.2 1,113,756 - +2.3 1,113,823 - -0.1 1,113,824 + +1.3 1,113,825 - -1.4 1,113,825 - -0.7 1,113,825 - +0.8 1,113,825 - +0.8 1,113,825 - -0.7 1,113,825 - -1.5 1,113,825 - +0.7 1,113,826 + -0.1 1,113,826 + +0.6 1,113,826 + +1.0 1,113,826 + +1.1 1,113,826 + +0.3 1,113,826 + -1.8 1,113,826 + -0.2 1,113,826 + +0.8 1,113,826 + +0.5 1,113,826 + +0.9 1,113,836 - +0.3 1,113,836 - +0.1 1,113,837 + -0.1 1,113,843 - +0.1 1,113,844 + -0.5 1,113,844 + +0.8 1,113,857 - -0.8 1,113,868 - +0.0 1,113,869 + +0.4 1,113,870 - +0.8 1,113,870 - -0.8 1,113,887 - -0.3 1,113,887 - -0.7 1,113,888 + +0.5 1,113,888 + +1.4 1,113,892 + +0.4 1,113,933 - +0.6 1,113,940 + -0.9 1,113,998 + MMJJ_RS05990 0.11 -0.8 1,113,998 + MMJJ_RS05990 0.11 -0.7 1,114,044 - MMJJ_RS05990 0.20 -2.6 1,114,045 + MMJJ_RS05990 0.20 +1.0 1,114,045 + MMJJ_RS05990 0.20 +1.1 1,114,045 + MMJJ_RS05990 0.20 -0.2 1,114,046 - MMJJ_RS05990 0.20 +0.1 1,114,046 - MMJJ_RS05990 0.20 +0.5 1,114,046 - MMJJ_RS05990 0.20 -0.5 1,114,046 - MMJJ_RS05990 0.20 +1.5 1,114,046 - MMJJ_RS05990 0.20 +0.0 1,114,046 - MMJJ_RS05990 0.20 +0.9 1,114,046 - MMJJ_RS05990 0.20 -1.7 1,114,046 - MMJJ_RS05990 0.20 +0.8 1,114,047 + MMJJ_RS05990 0.20 +0.9 1,114,113 - MMJJ_RS05990 0.32 -2.1 1,114,132 + MMJJ_RS05990 0.36 -0.7 1,114,140 - MMJJ_RS05990 0.37 +1.2 1,114,140 - MMJJ_RS05990 0.37 +0.4 1,114,140 - MMJJ_RS05990 0.37 +0.4 1,114,141 + MMJJ_RS05990 0.38 +0.7 1,114,141 + MMJJ_RS05990 0.38 +0.7 1,114,141 + MMJJ_RS05990 0.38 +1.0 1,114,143 - MMJJ_RS05990 0.38 +0.5 1,114,152 - MMJJ_RS05990 0.40 -0.5 1,114,164 - MMJJ_RS05990 0.42 +0.2 1,114,216 - MMJJ_RS05990 0.51 -1.0 1,114,350 - MMJJ_RS05990 0.76 -1.0 1,114,434 - -0.8 1,114,435 + +1.0 1,114,435 + +0.1 1,114,438 + +0.8 1,114,447 - -0.1 1,114,456 - -0.8 1,114,477 - +1.0 1,114,482 + -2.5 1,114,482 + +0.1 1,114,482 + +0.9 1,114,498 + +0.4 1,114,580 + -0.9 1,115,372 + MMJJ_RS05995 0.54 -3.1 1,115,444 + MMJJ_RS05995 0.58 -3.7 1,115,638 - MMJJ_RS05995 0.70 -2.6 1,115,639 + MMJJ_RS05995 0.70 -1.5 1,115,870 - MMJJ_RS05995 0.84 -1.2 1,116,046 - -1.7 1,116,049 + -2.0 1,116,049 + -1.9 1,116,161 - -3.5 1,116,168 + -1.6 1,116,168 + -2.4 1,116,178 + -2.7 1,116,178 + -1.9 1,116,179 - -0.6 1,116,179 - -2.9 1,116,180 + -1.7 1,116,181 - -2.1 1,116,181 - -2.0 1,116,181 - -0.5 1,116,181 - -1.9 1,116,181 - +0.0 1,116,181 - -0.3 1,116,182 + -2.2 1,116,183 - -1.9 1,116,183 - -2.0 1,116,184 + -3.1 1,116,184 + -0.4 1,116,184 + -2.9 1,116,184 + -0.9 1,116,184 + -2.3 1,116,184 + -3.5 1,116,184 + +0.1 1,116,196 + -2.0 1,116,201 - -1.4 1,116,201 - +0.1 1,116,206 + -3.1 1,116,206 + -2.3 1,116,211 - +0.5 1,116,213 - -2.6 1,116,284 - -3.3 1,116,313 - -2.8 1,116,313 - -1.1 1,116,314 + -0.5 1,116,329 + MMJJ_RS06000 0.11 -1.0 1,116,345 - MMJJ_RS06000 0.16 -2.7 1,116,346 + MMJJ_RS06000 0.16 -0.7 1,116,438 - MMJJ_RS06000 0.42 -1.5 1,116,462 + MMJJ_RS06000 0.49 -2.4 1,116,534 + MMJJ_RS06000 0.69 -0.8 1,116,587 + MMJJ_RS06000 0.84 -1.1 1,116,587 + MMJJ_RS06000 0.84 -1.0 1,116,677 - -0.9 1,116,677 - -2.0 1,116,702 - -1.7 1,116,703 + -0.7 1,116,745 + +0.1 1,116,768 - -1.9 1,116,768 - -3.2 1,116,768 - -2.2 1,116,769 + -2.3 1,116,769 + -1.3 1,116,769 + -2.5 1,116,782 - -2.0 1,116,782 - -0.9 1,116,820 - MMJJ_RS06005 0.10 -3.0 1,116,878 + MMJJ_RS06005 0.14 -2.0 1,116,898 + MMJJ_RS06005 0.16 -1.0 1,117,045 - MMJJ_RS06005 0.26 -1.4 1,117,046 + -0.9
Or see this region's nucleotide sequence