Experiment: H2 N-free with 2g/L casaminos
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS05700 and MMJJ_RS05705 are separated by 0 nucleotides MMJJ_RS05705 and MMJJ_RS05710 are separated by 132 nucleotides MMJJ_RS05710 and MMJJ_RS05715 are separated by 48 nucleotides
MMJJ_RS05700: MMJJ_RS05700 - metallophosphoesterase, at 1,061,753 to 1,062,436
_RS05700
MMJJ_RS05705: MMJJ_RS05705 - hypothetical protein, at 1,062,437 to 1,063,078
_RS05705
MMJJ_RS05710: MMJJ_RS05710 - single-stranded-DNA-specific exonuclease RecJ, at 1,063,211 to 1,064,614
_RS05710
MMJJ_RS05715: MMJJ_RS05715 - phosphoadenosine phosphosulfate reductase family protein, at 1,064,663 to 1,065,949
_RS05715
Position (kb)
1063
1064
1065 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1062.221 kb on - strand, within MMJJ_RS05700 at 1062.222 kb on + strand, within MMJJ_RS05700 at 1062.222 kb on + strand, within MMJJ_RS05700 at 1062.222 kb on + strand, within MMJJ_RS05700 at 1062.223 kb on - strand, within MMJJ_RS05700 at 1062.223 kb on - strand, within MMJJ_RS05700 at 1062.224 kb on + strand, within MMJJ_RS05700 at 1062.224 kb on + strand, within MMJJ_RS05700 at 1062.280 kb on - strand, within MMJJ_RS05700 at 1062.304 kb on + strand, within MMJJ_RS05700 at 1062.331 kb on - strand, within MMJJ_RS05700 at 1062.331 kb on - strand, within MMJJ_RS05700 at 1062.332 kb on + strand, within MMJJ_RS05700 at 1062.338 kb on + strand, within MMJJ_RS05700 at 1062.367 kb on + strand, within MMJJ_RS05700 at 1062.393 kb on - strand at 1062.393 kb on - strand at 1062.394 kb on + strand at 1062.394 kb on + strand at 1062.395 kb on - strand at 1062.395 kb on - strand at 1062.395 kb on - strand at 1062.396 kb on + strand at 1062.396 kb on + strand at 1062.396 kb on + strand at 1062.417 kb on - strand at 1062.423 kb on - strand at 1062.423 kb on - strand at 1062.424 kb on + strand at 1062.432 kb on - strand at 1062.433 kb on + strand at 1062.433 kb on + strand at 1062.435 kb on + strand at 1062.491 kb on + strand at 1062.506 kb on - strand, within MMJJ_RS05705 at 1062.507 kb on + strand, within MMJJ_RS05705 at 1062.507 kb on + strand, within MMJJ_RS05705 at 1062.514 kb on + strand, within MMJJ_RS05705 at 1062.553 kb on + strand, within MMJJ_RS05705 at 1062.570 kb on + strand, within MMJJ_RS05705 at 1062.570 kb on + strand, within MMJJ_RS05705 at 1062.570 kb on + strand, within MMJJ_RS05705 at 1062.571 kb on - strand, within MMJJ_RS05705 at 1062.572 kb on + strand, within MMJJ_RS05705 at 1062.575 kb on - strand, within MMJJ_RS05705 at 1062.578 kb on - strand, within MMJJ_RS05705 at 1062.579 kb on + strand, within MMJJ_RS05705 at 1062.581 kb on - strand, within MMJJ_RS05705 at 1062.581 kb on - strand, within MMJJ_RS05705 at 1062.581 kb on - strand, within MMJJ_RS05705 at 1062.581 kb on - strand, within MMJJ_RS05705 at 1062.585 kb on + strand, within MMJJ_RS05705 at 1062.607 kb on + strand, within MMJJ_RS05705 at 1062.613 kb on - strand, within MMJJ_RS05705 at 1062.618 kb on - strand, within MMJJ_RS05705 at 1062.722 kb on - strand, within MMJJ_RS05705 at 1062.723 kb on + strand, within MMJJ_RS05705 at 1062.727 kb on - strand, within MMJJ_RS05705 at 1062.729 kb on - strand, within MMJJ_RS05705 at 1062.730 kb on + strand, within MMJJ_RS05705 at 1062.732 kb on + strand, within MMJJ_RS05705 at 1062.732 kb on + strand, within MMJJ_RS05705 at 1062.765 kb on + strand, within MMJJ_RS05705 at 1062.779 kb on + strand, within MMJJ_RS05705 at 1062.780 kb on - strand, within MMJJ_RS05705 at 1062.780 kb on - strand, within MMJJ_RS05705 at 1062.780 kb on - strand, within MMJJ_RS05705 at 1062.781 kb on + strand, within MMJJ_RS05705 at 1062.781 kb on + strand, within MMJJ_RS05705 at 1062.781 kb on + strand, within MMJJ_RS05705 at 1062.783 kb on - strand, within MMJJ_RS05705 at 1062.850 kb on + strand, within MMJJ_RS05705 at 1062.869 kb on - strand, within MMJJ_RS05705 at 1062.881 kb on + strand, within MMJJ_RS05705 at 1062.911 kb on + strand, within MMJJ_RS05705 at 1062.917 kb on - strand, within MMJJ_RS05705 at 1062.932 kb on - strand, within MMJJ_RS05705 at 1062.950 kb on - strand, within MMJJ_RS05705 at 1062.954 kb on - strand, within MMJJ_RS05705 at 1062.955 kb on + strand, within MMJJ_RS05705 at 1062.955 kb on + strand, within MMJJ_RS05705 at 1062.959 kb on + strand, within MMJJ_RS05705 at 1062.961 kb on - strand, within MMJJ_RS05705 at 1062.962 kb on + strand, within MMJJ_RS05705 at 1062.990 kb on - strand, within MMJJ_RS05705 at 1062.990 kb on - strand, within MMJJ_RS05705 at 1062.991 kb on + strand, within MMJJ_RS05705 at 1062.991 kb on + strand, within MMJJ_RS05705 at 1062.994 kb on + strand, within MMJJ_RS05705 at 1063.025 kb on - strand at 1063.025 kb on - strand at 1063.025 kb on - strand at 1063.026 kb on + strand at 1063.029 kb on - strand at 1063.030 kb on + strand at 1063.034 kb on - strand at 1063.034 kb on - strand at 1063.034 kb on - strand at 1063.034 kb on - strand at 1063.035 kb on + strand at 1063.035 kb on + strand at 1063.035 kb on + strand at 1063.035 kb on + strand at 1063.037 kb on - strand at 1063.037 kb on - strand at 1063.037 kb on - strand at 1063.038 kb on + strand at 1063.038 kb on + strand at 1063.038 kb on + strand at 1063.061 kb on - strand at 1063.061 kb on - strand at 1063.061 kb on - strand at 1063.061 kb on - strand at 1063.062 kb on + strand at 1063.062 kb on + strand at 1063.062 kb on + strand at 1063.062 kb on + strand at 1063.072 kb on + strand at 1063.112 kb on - strand at 1063.113 kb on + strand at 1063.113 kb on + strand at 1063.114 kb on - strand at 1063.115 kb on + strand at 1063.115 kb on + strand at 1063.115 kb on + strand at 1063.115 kb on + strand at 1063.115 kb on + strand at 1063.115 kb on + strand at 1063.118 kb on - strand at 1063.118 kb on - strand at 1063.118 kb on - strand at 1063.118 kb on - strand at 1063.119 kb on + strand at 1063.119 kb on + strand at 1063.119 kb on + strand at 1063.120 kb on - strand at 1063.120 kb on - strand at 1063.121 kb on + strand at 1063.121 kb on + strand at 1063.121 kb on + strand at 1063.121 kb on + strand at 1063.121 kb on + strand at 1063.121 kb on + strand at 1063.121 kb on + strand at 1063.123 kb on + strand at 1063.137 kb on + strand at 1063.138 kb on - strand at 1063.138 kb on - strand at 1063.140 kb on - strand at 1063.141 kb on + strand at 1063.147 kb on - strand at 1063.168 kb on + strand at 1063.169 kb on - strand at 1063.169 kb on - strand at 1063.185 kb on + strand at 1063.185 kb on + strand at 1063.275 kb on - strand at 1063.402 kb on - strand, within MMJJ_RS05710 at 1063.467 kb on - strand, within MMJJ_RS05710 at 1063.528 kb on + strand, within MMJJ_RS05710 at 1063.556 kb on + strand, within MMJJ_RS05710 at 1063.626 kb on + strand, within MMJJ_RS05710 at 1063.842 kb on + strand, within MMJJ_RS05710 at 1063.891 kb on - strand, within MMJJ_RS05710 at 1063.891 kb on - strand, within MMJJ_RS05710 at 1063.932 kb on - strand, within MMJJ_RS05710 at 1063.946 kb on - strand, within MMJJ_RS05710 at 1064.209 kb on - strand, within MMJJ_RS05710 at 1064.314 kb on - strand, within MMJJ_RS05710 at 1064.314 kb on - strand, within MMJJ_RS05710 at 1064.442 kb on - strand, within MMJJ_RS05710 at 1064.507 kb on - strand at 1064.507 kb on - strand at 1064.594 kb on - strand at 1064.595 kb on + strand at 1064.623 kb on + strand at 1064.639 kb on + strand at 1064.655 kb on - strand at 1064.663 kb on + strand at 1064.668 kb on - strand at 1064.669 kb on + strand at 1064.703 kb on - strand at 1064.752 kb on + strand at 1064.798 kb on - strand, within MMJJ_RS05715 at 1064.798 kb on - strand, within MMJJ_RS05715 at 1064.799 kb on + strand at 1064.847 kb on - strand, within MMJJ_RS05715 at 1064.879 kb on - strand, within MMJJ_RS05715 at 1064.880 kb on + strand, within MMJJ_RS05715 at 1064.880 kb on + strand, within MMJJ_RS05715 at 1065.070 kb on + strand, within MMJJ_RS05715 at 1065.096 kb on - strand, within MMJJ_RS05715 at 1065.123 kb on - strand, within MMJJ_RS05715 at 1065.129 kb on + strand, within MMJJ_RS05715 at 1065.134 kb on + strand, within MMJJ_RS05715 at 1065.141 kb on + strand, within MMJJ_RS05715 at 1065.265 kb on - strand, within MMJJ_RS05715 at 1065.266 kb on + strand, within MMJJ_RS05715 at 1065.341 kb on - strand, within MMJJ_RS05715 at 1065.387 kb on - strand, within MMJJ_RS05715 at 1065.466 kb on - strand, within MMJJ_RS05715 at 1065.471 kb on - strand, within MMJJ_RS05715 at 1065.488 kb on - strand, within MMJJ_RS05715 at 1065.493 kb on + strand, within MMJJ_RS05715 at 1065.493 kb on + strand, within MMJJ_RS05715 at 1065.523 kb on - strand, within MMJJ_RS05715
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 2g/L casaminos remove 1,062,221 - MMJJ_RS05700 0.68 +1.4 1,062,222 + MMJJ_RS05700 0.69 -0.0 1,062,222 + MMJJ_RS05700 0.69 -2.5 1,062,222 + MMJJ_RS05700 0.69 +1.1 1,062,223 - MMJJ_RS05700 0.69 +0.9 1,062,223 - MMJJ_RS05700 0.69 +1.0 1,062,224 + MMJJ_RS05700 0.69 +0.4 1,062,224 + MMJJ_RS05700 0.69 -0.2 1,062,280 - MMJJ_RS05700 0.77 -0.9 1,062,304 + MMJJ_RS05700 0.81 -1.5 1,062,331 - MMJJ_RS05700 0.85 +1.2 1,062,331 - MMJJ_RS05700 0.85 +0.5 1,062,332 + MMJJ_RS05700 0.85 +0.1 1,062,338 + MMJJ_RS05700 0.86 +1.3 1,062,367 + MMJJ_RS05700 0.90 +0.8 1,062,393 - +0.3 1,062,393 - -0.2 1,062,394 + -0.0 1,062,394 + -1.1 1,062,395 - +0.3 1,062,395 - +0.1 1,062,395 - -0.1 1,062,396 + -0.6 1,062,396 + +1.2 1,062,396 + +0.4 1,062,417 - -0.1 1,062,423 - +0.0 1,062,423 - +1.1 1,062,424 + +0.3 1,062,432 - +0.6 1,062,433 + -0.0 1,062,433 + +0.1 1,062,435 + +0.7 1,062,491 + -0.7 1,062,506 - MMJJ_RS05705 0.11 +0.4 1,062,507 + MMJJ_RS05705 0.11 -0.2 1,062,507 + MMJJ_RS05705 0.11 +0.2 1,062,514 + MMJJ_RS05705 0.12 +0.7 1,062,553 + MMJJ_RS05705 0.18 +0.2 1,062,570 + MMJJ_RS05705 0.21 -0.3 1,062,570 + MMJJ_RS05705 0.21 +0.3 1,062,570 + MMJJ_RS05705 0.21 -0.5 1,062,571 - MMJJ_RS05705 0.21 +0.0 1,062,572 + MMJJ_RS05705 0.21 +0.1 1,062,575 - MMJJ_RS05705 0.21 +0.9 1,062,578 - MMJJ_RS05705 0.22 -0.2 1,062,579 + MMJJ_RS05705 0.22 +1.6 1,062,581 - MMJJ_RS05705 0.22 +1.1 1,062,581 - MMJJ_RS05705 0.22 +0.9 1,062,581 - MMJJ_RS05705 0.22 +1.0 1,062,581 - MMJJ_RS05705 0.22 -0.2 1,062,585 + MMJJ_RS05705 0.23 +0.6 1,062,607 + MMJJ_RS05705 0.26 -0.9 1,062,613 - MMJJ_RS05705 0.27 +0.4 1,062,618 - MMJJ_RS05705 0.28 -0.4 1,062,722 - MMJJ_RS05705 0.44 -2.1 1,062,723 + MMJJ_RS05705 0.45 -0.2 1,062,727 - MMJJ_RS05705 0.45 -0.8 1,062,729 - MMJJ_RS05705 0.45 +1.1 1,062,730 + MMJJ_RS05705 0.46 -0.0 1,062,732 + MMJJ_RS05705 0.46 +0.4 1,062,732 + MMJJ_RS05705 0.46 -1.1 1,062,765 + MMJJ_RS05705 0.51 -0.5 1,062,779 + MMJJ_RS05705 0.53 +0.6 1,062,780 - MMJJ_RS05705 0.53 -0.2 1,062,780 - MMJJ_RS05705 0.53 +0.6 1,062,780 - MMJJ_RS05705 0.53 +0.4 1,062,781 + MMJJ_RS05705 0.54 +0.9 1,062,781 + MMJJ_RS05705 0.54 -0.4 1,062,781 + MMJJ_RS05705 0.54 +0.2 1,062,783 - MMJJ_RS05705 0.54 +1.0 1,062,850 + MMJJ_RS05705 0.64 -0.5 1,062,869 - MMJJ_RS05705 0.67 -0.3 1,062,881 + MMJJ_RS05705 0.69 -0.9 1,062,911 + MMJJ_RS05705 0.74 -0.2 1,062,917 - MMJJ_RS05705 0.75 +1.3 1,062,932 - MMJJ_RS05705 0.77 -0.5 1,062,950 - MMJJ_RS05705 0.80 +1.2 1,062,954 - MMJJ_RS05705 0.81 -1.2 1,062,955 + MMJJ_RS05705 0.81 +0.3 1,062,955 + MMJJ_RS05705 0.81 -0.7 1,062,959 + MMJJ_RS05705 0.81 -0.3 1,062,961 - MMJJ_RS05705 0.82 +0.6 1,062,962 + MMJJ_RS05705 0.82 -0.1 1,062,990 - MMJJ_RS05705 0.86 -0.9 1,062,990 - MMJJ_RS05705 0.86 +0.0 1,062,991 + MMJJ_RS05705 0.86 -0.5 1,062,991 + MMJJ_RS05705 0.86 +1.6 1,062,994 + MMJJ_RS05705 0.87 +0.3 1,063,025 - +2.3 1,063,025 - +0.3 1,063,025 - +0.1 1,063,026 + +0.7 1,063,029 - +0.1 1,063,030 + -0.2 1,063,034 - +0.9 1,063,034 - +0.9 1,063,034 - +0.6 1,063,034 - +0.6 1,063,035 + -1.1 1,063,035 + -0.2 1,063,035 + +0.5 1,063,035 + -0.2 1,063,037 - +0.9 1,063,037 - -0.9 1,063,037 - +0.3 1,063,038 + +1.2 1,063,038 + -1.5 1,063,038 + -1.0 1,063,061 - +1.5 1,063,061 - -0.1 1,063,061 - -0.3 1,063,061 - +1.0 1,063,062 + -0.3 1,063,062 + -0.4 1,063,062 + -0.6 1,063,062 + +0.1 1,063,072 + +0.4 1,063,112 - -1.6 1,063,113 + +0.5 1,063,113 + +1.0 1,063,114 - +0.4 1,063,115 + -0.2 1,063,115 + -0.0 1,063,115 + +1.1 1,063,115 + -0.9 1,063,115 + -0.9 1,063,115 + +0.4 1,063,118 - +0.8 1,063,118 - -0.6 1,063,118 - +1.6 1,063,118 - -2.5 1,063,119 + +0.6 1,063,119 + +0.7 1,063,119 + -0.9 1,063,120 - +0.2 1,063,120 - +0.5 1,063,121 + +0.6 1,063,121 + -0.3 1,063,121 + +1.3 1,063,121 + -0.8 1,063,121 + +0.6 1,063,121 + +0.4 1,063,121 + +0.6 1,063,123 + +1.2 1,063,137 + +1.0 1,063,138 - -0.6 1,063,138 - -0.7 1,063,140 - +0.7 1,063,141 + -0.3 1,063,147 - +0.2 1,063,168 + +0.5 1,063,169 - -0.5 1,063,169 - -0.6 1,063,185 + +0.4 1,063,185 + +0.0 1,063,275 - -2.2 1,063,402 - MMJJ_RS05710 0.14 -1.8 1,063,467 - MMJJ_RS05710 0.18 -1.6 1,063,528 + MMJJ_RS05710 0.23 -0.0 1,063,556 + MMJJ_RS05710 0.25 -1.8 1,063,626 + MMJJ_RS05710 0.30 +1.0 1,063,842 + MMJJ_RS05710 0.45 -1.8 1,063,891 - MMJJ_RS05710 0.48 -2.2 1,063,891 - MMJJ_RS05710 0.48 -1.6 1,063,932 - MMJJ_RS05710 0.51 -2.7 1,063,946 - MMJJ_RS05710 0.52 -2.2 1,064,209 - MMJJ_RS05710 0.71 -2.6 1,064,314 - MMJJ_RS05710 0.79 -0.8 1,064,314 - MMJJ_RS05710 0.79 -0.0 1,064,442 - MMJJ_RS05710 0.88 -2.0 1,064,507 - -1.2 1,064,507 - -2.0 1,064,594 - -1.8 1,064,595 + -1.8 1,064,623 + +0.5 1,064,639 + +0.1 1,064,655 - +0.2 1,064,663 + +0.0 1,064,668 - -1.3 1,064,669 + +0.4 1,064,703 - +1.2 1,064,752 + -0.5 1,064,798 - MMJJ_RS05715 0.10 +0.2 1,064,798 - MMJJ_RS05715 0.10 +0.1 1,064,799 + +0.4 1,064,847 - MMJJ_RS05715 0.14 -0.9 1,064,879 - MMJJ_RS05715 0.17 -1.0 1,064,880 + MMJJ_RS05715 0.17 -0.7 1,064,880 + MMJJ_RS05715 0.17 +1.9 1,065,070 + MMJJ_RS05715 0.32 +1.0 1,065,096 - MMJJ_RS05715 0.34 -1.9 1,065,123 - MMJJ_RS05715 0.36 +0.4 1,065,129 + MMJJ_RS05715 0.36 -0.3 1,065,134 + MMJJ_RS05715 0.37 -0.5 1,065,141 + MMJJ_RS05715 0.37 -0.6 1,065,265 - MMJJ_RS05715 0.47 +0.7 1,065,266 + MMJJ_RS05715 0.47 -0.2 1,065,341 - MMJJ_RS05715 0.53 +0.3 1,065,387 - MMJJ_RS05715 0.56 -0.0 1,065,466 - MMJJ_RS05715 0.62 -1.8 1,065,471 - MMJJ_RS05715 0.63 +0.2 1,065,488 - MMJJ_RS05715 0.64 -0.3 1,065,493 + MMJJ_RS05715 0.64 +1.3 1,065,493 + MMJJ_RS05715 0.64 -0.4 1,065,523 - MMJJ_RS05715 0.67 -1.2
Or see this region's nucleotide sequence