Experiment: H2 N-free with 2g/L casaminos
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS05440 and MMJJ_RS05445 are separated by 38 nucleotides MMJJ_RS05445 and MMJJ_RS05450 are separated by 35 nucleotides
MMJJ_RS05440: MMJJ_RS05440 - serine/threonine protein kinase, at 1,012,134 to 1,013,030
_RS05440
MMJJ_RS05445: MMJJ_RS05445 - 2-oxoacid:acceptor oxidoreductase subunit alpha, at 1,013,069 to 1,014,160
_RS05445
MMJJ_RS05450: MMJJ_RS05450 - TIGR03576 family pyridoxal phosphate-dependent enzyme, at 1,014,196 to 1,015,260
_RS05450
Position (kb)
1013
1014
1015 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1
2 at 1012.069 kb on + strand at 1012.069 kb on + strand at 1012.076 kb on - strand at 1012.077 kb on + strand at 1012.080 kb on + strand at 1012.080 kb on + strand at 1012.080 kb on + strand at 1012.080 kb on + strand at 1012.118 kb on + strand at 1012.278 kb on + strand, within MMJJ_RS05440 at 1012.326 kb on - strand, within MMJJ_RS05440 at 1012.327 kb on + strand, within MMJJ_RS05440 at 1012.337 kb on + strand, within MMJJ_RS05440 at 1012.352 kb on - strand, within MMJJ_RS05440 at 1012.355 kb on + strand, within MMJJ_RS05440 at 1012.367 kb on + strand, within MMJJ_RS05440 at 1012.416 kb on - strand, within MMJJ_RS05440 at 1012.520 kb on - strand, within MMJJ_RS05440 at 1012.657 kb on - strand, within MMJJ_RS05440 at 1012.658 kb on + strand, within MMJJ_RS05440 at 1012.673 kb on + strand, within MMJJ_RS05440 at 1012.673 kb on + strand, within MMJJ_RS05440 at 1012.696 kb on - strand, within MMJJ_RS05440 at 1012.696 kb on - strand, within MMJJ_RS05440 at 1012.742 kb on - strand, within MMJJ_RS05440 at 1012.752 kb on - strand, within MMJJ_RS05440 at 1012.752 kb on - strand, within MMJJ_RS05440 at 1012.767 kb on + strand, within MMJJ_RS05440 at 1012.767 kb on + strand, within MMJJ_RS05440 at 1012.780 kb on - strand, within MMJJ_RS05440 at 1012.780 kb on - strand, within MMJJ_RS05440 at 1012.781 kb on + strand, within MMJJ_RS05440 at 1012.793 kb on - strand, within MMJJ_RS05440 at 1012.793 kb on - strand, within MMJJ_RS05440 at 1012.879 kb on - strand, within MMJJ_RS05440 at 1012.880 kb on + strand, within MMJJ_RS05440 at 1012.894 kb on - strand, within MMJJ_RS05440 at 1012.899 kb on - strand, within MMJJ_RS05440 at 1012.900 kb on + strand, within MMJJ_RS05440 at 1012.900 kb on + strand, within MMJJ_RS05440 at 1012.918 kb on - strand, within MMJJ_RS05440 at 1012.918 kb on - strand, within MMJJ_RS05440 at 1012.919 kb on + strand, within MMJJ_RS05440 at 1012.919 kb on + strand, within MMJJ_RS05440 at 1012.928 kb on - strand, within MMJJ_RS05440 at 1012.947 kb on - strand at 1012.952 kb on - strand at 1012.982 kb on - strand at 1012.982 kb on - strand at 1012.983 kb on + strand at 1012.988 kb on + strand at 1012.989 kb on - strand at 1012.989 kb on - strand at 1012.991 kb on - strand at 1012.996 kb on + strand at 1013.009 kb on - strand at 1013.012 kb on + strand at 1013.029 kb on + strand at 1013.081 kb on + strand at 1013.122 kb on + strand at 1013.725 kb on - strand at 1013.847 kb on - strand at 1013.847 kb on - strand, within MMJJ_RS05445 at 1013.852 kb on - strand at 1013.864 kb on - strand, within MMJJ_RS05445 at 1013.864 kb on - strand at 1013.983 kb on + strand, within MMJJ_RS05445 at 1014.015 kb on - strand, within MMJJ_RS05445 at 1014.097 kb on + strand at 1014.126 kb on - strand at 1014.126 kb on - strand at 1014.144 kb on - strand at 1014.144 kb on - strand at 1014.145 kb on + strand at 1014.145 kb on + strand at 1014.145 kb on + strand at 1014.145 kb on + strand at 1014.176 kb on - strand at 1014.192 kb on - strand at 1014.193 kb on + strand at 1014.197 kb on - strand at 1014.212 kb on + strand at 1014.223 kb on - strand at 1014.223 kb on - strand at 1014.223 kb on - strand at 1014.223 kb on - strand at 1014.224 kb on + strand at 1014.226 kb on - strand at 1014.226 kb on - strand at 1014.227 kb on + strand at 1014.230 kb on - strand at 1014.230 kb on - strand at 1014.231 kb on + strand at 1014.271 kb on - strand at 1014.272 kb on + strand at 1014.277 kb on - strand at 1014.285 kb on - strand at 1014.300 kb on - strand at 1014.300 kb on - strand at 1014.300 kb on - strand at 1014.318 kb on - strand, within MMJJ_RS05450 at 1014.322 kb on - strand, within MMJJ_RS05450 at 1014.322 kb on - strand, within MMJJ_RS05450 at 1014.322 kb on - strand, within MMJJ_RS05450 at 1014.322 kb on - strand, within MMJJ_RS05450 at 1014.323 kb on + strand, within MMJJ_RS05450 at 1014.323 kb on + strand, within MMJJ_RS05450 at 1014.386 kb on + strand, within MMJJ_RS05450 at 1014.388 kb on - strand at 1014.390 kb on - strand, within MMJJ_RS05450 at 1014.390 kb on - strand, within MMJJ_RS05450 at 1014.391 kb on + strand, within MMJJ_RS05450 at 1014.436 kb on - strand, within MMJJ_RS05450 at 1014.436 kb on - strand, within MMJJ_RS05450 at 1014.437 kb on + strand, within MMJJ_RS05450 at 1014.442 kb on - strand, within MMJJ_RS05450 at 1014.443 kb on + strand, within MMJJ_RS05450 at 1014.483 kb on - strand, within MMJJ_RS05450 at 1014.487 kb on - strand, within MMJJ_RS05450 at 1014.487 kb on - strand, within MMJJ_RS05450 at 1014.487 kb on - strand, within MMJJ_RS05450 at 1014.488 kb on + strand, within MMJJ_RS05450 at 1014.511 kb on - strand, within MMJJ_RS05450 at 1014.512 kb on + strand, within MMJJ_RS05450 at 1014.512 kb on + strand, within MMJJ_RS05450 at 1014.512 kb on + strand, within MMJJ_RS05450 at 1014.569 kb on - strand, within MMJJ_RS05450 at 1014.569 kb on - strand, within MMJJ_RS05450 at 1014.569 kb on - strand, within MMJJ_RS05450 at 1014.570 kb on + strand, within MMJJ_RS05450 at 1014.571 kb on - strand, within MMJJ_RS05450 at 1014.571 kb on - strand, within MMJJ_RS05450 at 1014.571 kb on - strand, within MMJJ_RS05450 at 1014.583 kb on - strand, within MMJJ_RS05450 at 1014.629 kb on + strand, within MMJJ_RS05450 at 1014.652 kb on - strand, within MMJJ_RS05450 at 1014.689 kb on - strand, within MMJJ_RS05450 at 1014.761 kb on + strand, within MMJJ_RS05450 at 1014.764 kb on + strand, within MMJJ_RS05450 at 1014.790 kb on - strand, within MMJJ_RS05450 at 1014.791 kb on + strand, within MMJJ_RS05450 at 1014.944 kb on - strand, within MMJJ_RS05450 at 1015.021 kb on - strand, within MMJJ_RS05450 at 1015.022 kb on + strand, within MMJJ_RS05450 at 1015.022 kb on + strand, within MMJJ_RS05450 at 1015.030 kb on + strand, within MMJJ_RS05450 at 1015.051 kb on - strand, within MMJJ_RS05450 at 1015.071 kb on - strand, within MMJJ_RS05450 at 1015.072 kb on + strand, within MMJJ_RS05450 at 1015.075 kb on - strand, within MMJJ_RS05450 at 1015.106 kb on - strand, within MMJJ_RS05450 at 1015.106 kb on - strand, within MMJJ_RS05450 at 1015.106 kb on - strand, within MMJJ_RS05450 at 1015.106 kb on - strand, within MMJJ_RS05450 at 1015.107 kb on + strand, within MMJJ_RS05450 at 1015.107 kb on + strand, within MMJJ_RS05450 at 1015.107 kb on + strand, within MMJJ_RS05450 at 1015.107 kb on + strand, within MMJJ_RS05450 at 1015.107 kb on + strand, within MMJJ_RS05450 at 1015.107 kb on + strand, within MMJJ_RS05450 at 1015.114 kb on - strand, within MMJJ_RS05450
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 2g/L casaminos remove 1,012,069 + -0.5 1,012,069 + -1.5 1,012,076 - -0.8 1,012,077 + -0.4 1,012,080 + -0.3 1,012,080 + +1.2 1,012,080 + -2.0 1,012,080 + -0.4 1,012,118 + -2.4 1,012,278 + MMJJ_RS05440 0.16 -0.6 1,012,326 - MMJJ_RS05440 0.21 +0.1 1,012,327 + MMJJ_RS05440 0.22 -2.6 1,012,337 + MMJJ_RS05440 0.23 -0.3 1,012,352 - MMJJ_RS05440 0.24 +1.1 1,012,355 + MMJJ_RS05440 0.25 -0.9 1,012,367 + MMJJ_RS05440 0.26 +0.3 1,012,416 - MMJJ_RS05440 0.31 +0.2 1,012,520 - MMJJ_RS05440 0.43 -0.4 1,012,657 - MMJJ_RS05440 0.58 +0.6 1,012,658 + MMJJ_RS05440 0.58 +0.3 1,012,673 + MMJJ_RS05440 0.60 -0.9 1,012,673 + MMJJ_RS05440 0.60 +0.3 1,012,696 - MMJJ_RS05440 0.63 +0.1 1,012,696 - MMJJ_RS05440 0.63 +0.8 1,012,742 - MMJJ_RS05440 0.68 -0.8 1,012,752 - MMJJ_RS05440 0.69 -0.4 1,012,752 - MMJJ_RS05440 0.69 +0.4 1,012,767 + MMJJ_RS05440 0.71 +1.1 1,012,767 + MMJJ_RS05440 0.71 -1.1 1,012,780 - MMJJ_RS05440 0.72 -0.7 1,012,780 - MMJJ_RS05440 0.72 -1.3 1,012,781 + MMJJ_RS05440 0.72 -0.1 1,012,793 - MMJJ_RS05440 0.73 -1.2 1,012,793 - MMJJ_RS05440 0.73 +0.3 1,012,879 - MMJJ_RS05440 0.83 -1.8 1,012,880 + MMJJ_RS05440 0.83 -1.0 1,012,894 - MMJJ_RS05440 0.85 +0.4 1,012,899 - MMJJ_RS05440 0.85 -1.1 1,012,900 + MMJJ_RS05440 0.85 -1.0 1,012,900 + MMJJ_RS05440 0.85 +0.6 1,012,918 - MMJJ_RS05440 0.87 -0.3 1,012,918 - MMJJ_RS05440 0.87 -2.4 1,012,919 + MMJJ_RS05440 0.88 +2.0 1,012,919 + MMJJ_RS05440 0.88 -0.9 1,012,928 - MMJJ_RS05440 0.89 -0.6 1,012,947 - -1.1 1,012,952 - +0.6 1,012,982 - -2.7 1,012,982 - -2.3 1,012,983 + -1.3 1,012,988 + -1.9 1,012,989 - -1.5 1,012,989 - -1.5 1,012,991 - -1.3 1,012,996 + -1.8 1,013,009 - -1.9 1,013,012 + -0.7 1,013,029 + -2.5 1,013,081 + -1.3 1,013,122 + -1.5 1,013,725 - -0.5 1,013,847 - -1.3 1,013,847 - MMJJ_RS05445 0.71 -3.6 1,013,852 - -1.3 1,013,864 - MMJJ_RS05445 0.73 -2.8 1,013,864 - -1.3 1,013,983 + MMJJ_RS05445 0.84 -0.9 1,014,015 - MMJJ_RS05445 0.87 -2.3 1,014,097 + -1.3 1,014,126 - -0.9 1,014,126 - +0.5 1,014,144 - +1.0 1,014,144 - +1.1 1,014,145 + -0.1 1,014,145 + +0.1 1,014,145 + -0.3 1,014,145 + -0.0 1,014,176 - -0.5 1,014,192 - +0.1 1,014,193 + +0.4 1,014,197 - -0.2 1,014,212 + -3.3 1,014,223 - -2.3 1,014,223 - -3.9 1,014,223 - -5.7 1,014,223 - -5.2 1,014,224 + -4.1 1,014,226 - -4.2 1,014,226 - -2.5 1,014,227 + -6.0 1,014,230 - -4.9 1,014,230 - -3.3 1,014,231 + -3.9 1,014,271 - -4.9 1,014,272 + -4.0 1,014,277 - -3.2 1,014,285 - -3.2 1,014,300 - -4.8 1,014,300 - -1.5 1,014,300 - -4.0 1,014,318 - MMJJ_RS05450 0.11 -0.5 1,014,322 - MMJJ_RS05450 0.12 -5.1 1,014,322 - MMJJ_RS05450 0.12 -5.2 1,014,322 - MMJJ_RS05450 0.12 -3.9 1,014,322 - MMJJ_RS05450 0.12 -5.0 1,014,323 + MMJJ_RS05450 0.12 -3.4 1,014,323 + MMJJ_RS05450 0.12 -5.5 1,014,386 + MMJJ_RS05450 0.18 -4.0 1,014,388 - -0.5 1,014,390 - MMJJ_RS05450 0.18 -4.2 1,014,390 - MMJJ_RS05450 0.18 -2.5 1,014,391 + MMJJ_RS05450 0.18 -3.6 1,014,436 - MMJJ_RS05450 0.23 -3.8 1,014,436 - MMJJ_RS05450 0.23 -5.4 1,014,437 + MMJJ_RS05450 0.23 -3.9 1,014,442 - MMJJ_RS05450 0.23 -3.8 1,014,443 + MMJJ_RS05450 0.23 -4.8 1,014,483 - MMJJ_RS05450 0.27 -3.6 1,014,487 - MMJJ_RS05450 0.27 -4.2 1,014,487 - MMJJ_RS05450 0.27 -1.2 1,014,487 - MMJJ_RS05450 0.27 -5.2 1,014,488 + MMJJ_RS05450 0.27 -1.8 1,014,511 - MMJJ_RS05450 0.30 -4.1 1,014,512 + MMJJ_RS05450 0.30 -3.8 1,014,512 + MMJJ_RS05450 0.30 -4.3 1,014,512 + MMJJ_RS05450 0.30 -4.0 1,014,569 - MMJJ_RS05450 0.35 -2.5 1,014,569 - MMJJ_RS05450 0.35 -4.1 1,014,569 - MMJJ_RS05450 0.35 -2.8 1,014,570 + MMJJ_RS05450 0.35 -4.5 1,014,571 - MMJJ_RS05450 0.35 -2.1 1,014,571 - MMJJ_RS05450 0.35 -4.9 1,014,571 - MMJJ_RS05450 0.35 -5.7 1,014,583 - MMJJ_RS05450 0.36 -4.4 1,014,629 + MMJJ_RS05450 0.41 -0.5 1,014,652 - MMJJ_RS05450 0.43 -3.9 1,014,689 - MMJJ_RS05450 0.46 -5.4 1,014,761 + MMJJ_RS05450 0.53 -0.3 1,014,764 + MMJJ_RS05450 0.53 -5.1 1,014,790 - MMJJ_RS05450 0.56 -4.6 1,014,791 + MMJJ_RS05450 0.56 -3.8 1,014,944 - MMJJ_RS05450 0.70 -5.5 1,015,021 - MMJJ_RS05450 0.77 -2.5 1,015,022 + MMJJ_RS05450 0.78 -3.8 1,015,022 + MMJJ_RS05450 0.78 -4.7 1,015,030 + MMJJ_RS05450 0.78 -5.7 1,015,051 - MMJJ_RS05450 0.80 -5.7 1,015,071 - MMJJ_RS05450 0.82 -4.1 1,015,072 + MMJJ_RS05450 0.82 -5.4 1,015,075 - MMJJ_RS05450 0.83 -4.7 1,015,106 - MMJJ_RS05450 0.85 -3.5 1,015,106 - MMJJ_RS05450 0.85 -3.9 1,015,106 - MMJJ_RS05450 0.85 -4.4 1,015,106 - MMJJ_RS05450 0.85 -3.5 1,015,107 + MMJJ_RS05450 0.86 -3.4 1,015,107 + MMJJ_RS05450 0.86 -5.5 1,015,107 + MMJJ_RS05450 0.86 -4.2 1,015,107 + MMJJ_RS05450 0.86 -3.5 1,015,107 + MMJJ_RS05450 0.86 -3.5 1,015,107 + MMJJ_RS05450 0.86 -3.8 1,015,114 - MMJJ_RS05450 0.86 -1.5
Or see this region's nucleotide sequence