Experiment: H2 N-free with 2g/L casaminos
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS05250 and MMJJ_RS05255 are separated by 10 nucleotides MMJJ_RS05255 and MMJJ_RS05260 are separated by 3 nucleotides MMJJ_RS05260 and MMJJ_RS05265 are separated by 25 nucleotides MMJJ_RS05265 and MMJJ_RS05270 are separated by 20 nucleotides
MMJJ_RS05250: MMJJ_RS05250 - HDIG domain-containing protein, at 970,708 to 971,295
_RS05250
MMJJ_RS05255: MMJJ_RS05255 - transcription factor E, at 971,306 to 971,863
_RS05255
MMJJ_RS05260: MMJJ_RS05260 - ATP-grasp domain-containing protein, at 971,867 to 972,916
_RS05260
MMJJ_RS05265: MMJJ_RS05265 - GTP cyclohydrolase MptA, at 972,942 to 973,889
_RS05265
MMJJ_RS05270: MMJJ_RS05270 - AAA family ATPase, at 973,910 to 975,136
_RS05270
Position (kb) 971
972
973 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 970.884 kb on + strand, within MMJJ_RS05250 at 970.993 kb on - strand, within MMJJ_RS05250 at 970.994 kb on + strand, within MMJJ_RS05250 at 971.020 kb on - strand, within MMJJ_RS05250 at 971.021 kb on + strand, within MMJJ_RS05250 at 971.021 kb on + strand, within MMJJ_RS05250 at 971.021 kb on + strand, within MMJJ_RS05250 at 971.021 kb on + strand, within MMJJ_RS05250 at 971.021 kb on + strand, within MMJJ_RS05250 at 971.034 kb on - strand, within MMJJ_RS05250 at 971.034 kb on - strand, within MMJJ_RS05250 at 971.035 kb on + strand, within MMJJ_RS05250 at 971.100 kb on - strand, within MMJJ_RS05250 at 971.155 kb on - strand, within MMJJ_RS05250 at 971.211 kb on - strand, within MMJJ_RS05250 at 971.212 kb on + strand, within MMJJ_RS05250 at 971.216 kb on + strand, within MMJJ_RS05250 at 971.254 kb on - strand at 971.254 kb on - strand at 971.254 kb on - strand at 971.255 kb on + strand at 971.255 kb on + strand at 971.259 kb on - strand at 971.290 kb on + strand at 971.296 kb on + strand at 971.310 kb on - strand at 971.311 kb on + strand at 971.311 kb on + strand at 971.333 kb on + strand at 971.336 kb on + strand at 971.357 kb on + strand at 971.741 kb on + strand, within MMJJ_RS05255 at 971.749 kb on - strand, within MMJJ_RS05255 at 972.182 kb on - strand, within MMJJ_RS05260 at 972.301 kb on - strand, within MMJJ_RS05260 at 972.402 kb on - strand at 972.407 kb on - strand, within MMJJ_RS05260 at 972.447 kb on + strand, within MMJJ_RS05260 at 972.480 kb on + strand at 972.480 kb on + strand, within MMJJ_RS05260 at 972.485 kb on - strand, within MMJJ_RS05260 at 972.533 kb on - strand, within MMJJ_RS05260 at 972.540 kb on - strand, within MMJJ_RS05260 at 972.541 kb on + strand, within MMJJ_RS05260 at 972.581 kb on - strand, within MMJJ_RS05260 at 972.596 kb on + strand, within MMJJ_RS05260 at 972.686 kb on - strand, within MMJJ_RS05260 at 972.731 kb on - strand, within MMJJ_RS05260 at 972.731 kb on - strand, within MMJJ_RS05260 at 972.750 kb on - strand, within MMJJ_RS05260 at 972.758 kb on - strand, within MMJJ_RS05260 at 972.788 kb on + strand, within MMJJ_RS05260 at 972.788 kb on + strand, within MMJJ_RS05260 at 972.793 kb on + strand, within MMJJ_RS05260 at 972.826 kb on - strand at 972.827 kb on + strand at 972.827 kb on + strand at 972.828 kb on - strand at 972.844 kb on + strand at 972.867 kb on + strand at 972.890 kb on + strand at 972.890 kb on + strand at 972.890 kb on + strand at 972.938 kb on + strand at 972.960 kb on + strand at 973.022 kb on - strand at 973.035 kb on - strand at 973.036 kb on + strand at 973.044 kb on - strand, within MMJJ_RS05265 at 973.556 kb on + strand, within MMJJ_RS05265 at 973.681 kb on + strand, within MMJJ_RS05265 at 973.854 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 2g/L casaminos remove 970,884 + MMJJ_RS05250 0.30 -0.6 970,993 - MMJJ_RS05250 0.48 -0.9 970,994 + MMJJ_RS05250 0.49 -0.7 971,020 - MMJJ_RS05250 0.53 -0.1 971,021 + MMJJ_RS05250 0.53 -1.1 971,021 + MMJJ_RS05250 0.53 -0.4 971,021 + MMJJ_RS05250 0.53 +0.2 971,021 + MMJJ_RS05250 0.53 -0.0 971,021 + MMJJ_RS05250 0.53 -0.3 971,034 - MMJJ_RS05250 0.55 -0.0 971,034 - MMJJ_RS05250 0.55 -1.5 971,035 + MMJJ_RS05250 0.56 -0.6 971,100 - MMJJ_RS05250 0.67 +1.3 971,155 - MMJJ_RS05250 0.76 -1.2 971,211 - MMJJ_RS05250 0.86 +1.0 971,212 + MMJJ_RS05250 0.86 +1.2 971,216 + MMJJ_RS05250 0.86 -0.0 971,254 - +0.9 971,254 - +0.9 971,254 - -1.0 971,255 + +0.6 971,255 + +0.1 971,259 - -0.1 971,290 + +0.2 971,296 + -0.0 971,310 - +0.6 971,311 + -1.1 971,311 + -1.3 971,333 + -1.3 971,336 + +0.8 971,357 + -1.1 971,741 + MMJJ_RS05255 0.78 +0.1 971,749 - MMJJ_RS05255 0.79 -1.0 972,182 - MMJJ_RS05260 0.30 -1.6 972,301 - MMJJ_RS05260 0.41 -2.6 972,402 - -1.0 972,407 - MMJJ_RS05260 0.51 -3.3 972,447 + MMJJ_RS05260 0.55 -1.5 972,480 + +1.0 972,480 + MMJJ_RS05260 0.58 -2.2 972,485 - MMJJ_RS05260 0.59 -2.6 972,533 - MMJJ_RS05260 0.63 -3.7 972,540 - MMJJ_RS05260 0.64 -2.5 972,541 + MMJJ_RS05260 0.64 -2.3 972,581 - MMJJ_RS05260 0.68 -3.0 972,596 + MMJJ_RS05260 0.69 -1.8 972,686 - MMJJ_RS05260 0.78 -0.4 972,731 - MMJJ_RS05260 0.82 -3.3 972,731 - MMJJ_RS05260 0.82 -2.3 972,750 - MMJJ_RS05260 0.84 -3.5 972,758 - MMJJ_RS05260 0.85 -3.9 972,788 + MMJJ_RS05260 0.88 -2.6 972,788 + MMJJ_RS05260 0.88 -3.9 972,793 + MMJJ_RS05260 0.88 -2.1 972,826 - -3.3 972,827 + -3.1 972,827 + -1.7 972,828 - -3.1 972,844 + -2.5 972,867 + -3.1 972,890 + -0.5 972,890 + -2.0 972,890 + -2.3 972,938 + -2.1 972,960 + -3.6 973,022 - -2.2 973,035 - -2.0 973,036 + -0.0 973,044 - MMJJ_RS05265 0.11 -1.6 973,556 + MMJJ_RS05265 0.65 -1.3 973,681 + MMJJ_RS05265 0.78 -1.7 973,854 + +0.3
Or see this region's nucleotide sequence