Experiment: H2 N-free with 2g/L casaminos
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS05220 and MMJJ_RS05225 are separated by 502 nucleotides MMJJ_RS05225 and MMJJ_RS05230 are separated by 18 nucleotides
MMJJ_RS05220: MMJJ_RS05220 - type 2 isopentenyl-diphosphate Delta-isomerase, at 964,162 to 965,229
_RS05220
MMJJ_RS05225: MMJJ_RS05225 - Gar1/Naf1 family protein, at 965,732 to 966,013
_RS05225
MMJJ_RS05230: MMJJ_RS05230 - transcription initiation factor IIB, at 966,032 to 967,051
_RS05230
Position (kb) 965
966
967 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 964.958 kb on + strand, within MMJJ_RS05220 at 964.980 kb on - strand at 965.160 kb on + strand at 965.245 kb on - strand at 965.246 kb on + strand at 965.246 kb on + strand at 965.256 kb on + strand at 965.294 kb on - strand at 965.294 kb on - strand at 965.295 kb on + strand at 965.301 kb on - strand at 965.340 kb on - strand at 965.341 kb on + strand at 965.341 kb on + strand at 965.341 kb on + strand at 965.341 kb on + strand at 965.341 kb on + strand at 965.343 kb on - strand at 965.343 kb on - strand at 965.343 kb on - strand at 965.343 kb on - strand at 965.344 kb on + strand at 965.344 kb on + strand at 965.349 kb on + strand at 965.354 kb on - strand at 965.354 kb on - strand at 965.355 kb on + strand at 965.361 kb on + strand at 965.375 kb on - strand at 965.403 kb on - strand at 965.416 kb on + strand at 965.420 kb on + strand at 965.427 kb on + strand at 965.502 kb on - strand at 965.528 kb on - strand at 965.528 kb on - strand at 965.529 kb on + strand at 965.553 kb on - strand at 965.554 kb on + strand at 965.554 kb on + strand at 965.554 kb on + strand at 965.554 kb on + strand at 965.557 kb on - strand at 965.557 kb on - strand at 965.557 kb on - strand at 965.557 kb on - strand at 965.558 kb on + strand at 965.558 kb on + strand at 965.558 kb on + strand at 965.558 kb on + strand at 965.558 kb on + strand at 965.585 kb on - strand at 965.585 kb on - strand at 965.586 kb on + strand at 965.614 kb on + strand at 965.614 kb on + strand at 965.615 kb on - strand at 965.616 kb on + strand at 965.617 kb on - strand at 965.619 kb on - strand at 965.620 kb on + strand at 965.633 kb on - strand at 965.635 kb on - strand at 965.636 kb on + strand at 965.639 kb on - strand at 965.660 kb on - strand at 965.660 kb on - strand at 965.706 kb on - strand at 965.706 kb on - strand at 965.824 kb on + strand at 965.843 kb on + strand, within MMJJ_RS05225 at 965.891 kb on - strand at 965.895 kb on - strand, within MMJJ_RS05225 at 966.250 kb on + strand, within MMJJ_RS05230
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 2g/L casaminos remove 964,958 + MMJJ_RS05220 0.75 -1.6 964,980 - +1.0 965,160 + -2.0 965,245 - -1.6 965,246 + -2.6 965,246 + -0.6 965,256 + -1.4 965,294 - -3.1 965,294 - -0.9 965,295 + -0.3 965,301 - +0.6 965,340 - -1.0 965,341 + -0.4 965,341 + +0.2 965,341 + +0.5 965,341 + -0.4 965,341 + +0.1 965,343 - +0.0 965,343 - -0.8 965,343 - -2.2 965,343 - +1.2 965,344 + +0.3 965,344 + -0.2 965,349 + +1.0 965,354 - +1.1 965,354 - +1.4 965,355 + +0.5 965,361 + +0.1 965,375 - +0.8 965,403 - +0.9 965,416 + -1.4 965,420 + -1.4 965,427 + -0.7 965,502 - +0.7 965,528 - +0.1 965,528 - -0.0 965,529 + +0.4 965,553 - -0.2 965,554 + -1.6 965,554 + +0.5 965,554 + -0.1 965,554 + -1.7 965,557 - +0.0 965,557 - -1.2 965,557 - +0.4 965,557 - +0.2 965,558 + -1.4 965,558 + -0.1 965,558 + -0.4 965,558 + +0.4 965,558 + +0.5 965,585 - -2.4 965,585 - +0.3 965,586 + -0.0 965,614 + -1.4 965,614 + -0.7 965,615 - +0.3 965,616 + -0.0 965,617 - -0.4 965,619 - -0.4 965,620 + +0.9 965,633 - -0.1 965,635 - -0.7 965,636 + -0.7 965,639 - -1.5 965,660 - -3.3 965,660 - -2.4 965,706 - -1.8 965,706 - -1.6 965,824 + -1.4 965,843 + MMJJ_RS05225 0.39 -4.6 965,891 - +1.0 965,895 - MMJJ_RS05225 0.58 -1.6 966,250 + MMJJ_RS05230 0.21 -2.5
Or see this region's nucleotide sequence