Experiment: H2 N-free with 2g/L casaminos
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS05065 and MMJJ_RS05070 are separated by 114 nucleotides MMJJ_RS05070 and MMJJ_RS05075 are separated by 67 nucleotides MMJJ_RS05075 and MMJJ_RS05080 are separated by 259 nucleotides MMJJ_RS05080 and MMJJ_RS05085 are separated by 44 nucleotides
MMJJ_RS05065: MMJJ_RS05065 - (4-{4-[2-(gamma-L- glutamylamino)ethyl]phenoxymethyl}furan-2-yl)methanamine synthase, at 940,832 to 941,812
_RS05065
MMJJ_RS05070: MMJJ_RS05070 - DNA primase catalytic subunit PriS, at 941,927 to 943,000
_RS05070
MMJJ_RS05075: MMJJ_RS05075 - tRNA-Gln, at 943,068 to 943,143
_RS05075
MMJJ_RS05080: MMJJ_RS05080 - tRNA-Thr, at 943,403 to 943,476
_RS05080
MMJJ_RS05085: MMJJ_RS05085 - hypothetical protein, at 943,521 to 944,459
_RS05085
Position (kb) 941
942
943
944 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 940.973 kb on - strand, within MMJJ_RS05065 at 941.027 kb on - strand, within MMJJ_RS05065 at 941.046 kb on - strand, within MMJJ_RS05065 at 941.063 kb on + strand, within MMJJ_RS05065 at 941.213 kb on + strand, within MMJJ_RS05065 at 941.222 kb on - strand, within MMJJ_RS05065 at 941.497 kb on + strand, within MMJJ_RS05065 at 941.696 kb on - strand, within MMJJ_RS05065 at 941.744 kb on + strand at 941.823 kb on - strand at 941.824 kb on + strand at 941.824 kb on + strand at 941.824 kb on + strand at 941.832 kb on + strand at 941.858 kb on - strand at 941.858 kb on - strand at 941.859 kb on + strand at 941.864 kb on - strand at 941.864 kb on - strand at 941.865 kb on + strand at 941.865 kb on + strand at 941.870 kb on - strand at 941.871 kb on + strand at 941.871 kb on + strand at 941.874 kb on - strand at 941.874 kb on - strand at 941.890 kb on - strand at 941.891 kb on + strand at 942.158 kb on + strand, within MMJJ_RS05070 at 942.254 kb on + strand, within MMJJ_RS05070 at 942.268 kb on - strand, within MMJJ_RS05070 at 942.390 kb on - strand, within MMJJ_RS05070 at 942.390 kb on - strand, within MMJJ_RS05070 at 942.417 kb on - strand at 943.030 kb on + strand at 943.062 kb on - strand at 943.180 kb on - strand at 943.264 kb on - strand at 943.265 kb on + strand at 943.265 kb on + strand at 943.271 kb on - strand at 943.334 kb on + strand at 943.485 kb on + strand at 943.563 kb on - strand at 943.563 kb on - strand at 943.564 kb on + strand at 943.564 kb on + strand at 943.572 kb on + strand at 943.689 kb on - strand, within MMJJ_RS05085 at 943.690 kb on + strand, within MMJJ_RS05085 at 943.702 kb on + strand, within MMJJ_RS05085 at 943.741 kb on + strand, within MMJJ_RS05085 at 943.742 kb on - strand, within MMJJ_RS05085 at 943.743 kb on + strand, within MMJJ_RS05085 at 943.905 kb on - strand, within MMJJ_RS05085 at 943.906 kb on + strand, within MMJJ_RS05085 at 943.953 kb on - strand, within MMJJ_RS05085 at 943.974 kb on + strand, within MMJJ_RS05085 at 943.984 kb on + strand, within MMJJ_RS05085
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 2g/L casaminos remove 940,973 - MMJJ_RS05065 0.14 -2.1 941,027 - MMJJ_RS05065 0.20 -2.8 941,046 - MMJJ_RS05065 0.22 -2.9 941,063 + MMJJ_RS05065 0.24 -1.2 941,213 + MMJJ_RS05065 0.39 -2.2 941,222 - MMJJ_RS05065 0.40 -2.1 941,497 + MMJJ_RS05065 0.68 -0.4 941,696 - MMJJ_RS05065 0.88 -3.8 941,744 + -1.1 941,823 - -1.2 941,824 + -1.7 941,824 + +0.4 941,824 + +0.1 941,832 + -1.8 941,858 - -0.5 941,858 - -1.6 941,859 + +0.7 941,864 - -0.2 941,864 - -0.5 941,865 + +1.2 941,865 + -0.9 941,870 - +1.1 941,871 + -0.3 941,871 + +0.9 941,874 - +0.5 941,874 - +1.3 941,890 - +0.8 941,891 + +0.5 942,158 + MMJJ_RS05070 0.22 -0.7 942,254 + MMJJ_RS05070 0.30 -2.9 942,268 - MMJJ_RS05070 0.32 -3.1 942,390 - MMJJ_RS05070 0.43 -2.2 942,390 - MMJJ_RS05070 0.43 -2.2 942,417 - -0.7 943,030 + -0.5 943,062 - +0.4 943,180 - -1.4 943,264 - +0.8 943,265 + +0.9 943,265 + -0.7 943,271 - -1.3 943,334 + -0.1 943,485 + -0.4 943,563 - -0.1 943,563 - +1.5 943,564 + +0.3 943,564 + +0.9 943,572 + -1.1 943,689 - MMJJ_RS05085 0.18 +0.3 943,690 + MMJJ_RS05085 0.18 -1.1 943,702 + MMJJ_RS05085 0.19 +0.8 943,741 + MMJJ_RS05085 0.23 -1.2 943,742 - MMJJ_RS05085 0.24 -0.8 943,743 + MMJJ_RS05085 0.24 -0.8 943,905 - MMJJ_RS05085 0.41 -0.1 943,906 + MMJJ_RS05085 0.41 +0.9 943,953 - MMJJ_RS05085 0.46 -0.7 943,974 + MMJJ_RS05085 0.48 -0.5 943,984 + MMJJ_RS05085 0.49 -0.5
Or see this region's nucleotide sequence