Experiment: H2 N-free with 2g/L casaminos
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS04645 and MMJJ_RS04650 are separated by 309 nucleotides MMJJ_RS04650 and MMJJ_RS04655 are separated by 14 nucleotides
MMJJ_RS04645: MMJJ_RS04645 - acetate--CoA ligase, at 865,680 to 867,590
_RS04645
MMJJ_RS04650: MMJJ_RS04650 - nitrogenase iron protein, at 867,900 to 868,778
_RS04650
MMJJ_RS04655: MMJJ_RS04655 - FAD-dependent oxidoreductase, at 868,793 to 870,199
_RS04655
Position (kb) 867
868
869 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 866.909 kb on - strand, within MMJJ_RS04645 at 866.910 kb on + strand, within MMJJ_RS04645 at 866.910 kb on + strand, within MMJJ_RS04645 at 866.991 kb on - strand, within MMJJ_RS04645 at 867.026 kb on - strand, within MMJJ_RS04645 at 867.067 kb on - strand, within MMJJ_RS04645 at 867.067 kb on - strand, within MMJJ_RS04645 at 867.068 kb on + strand, within MMJJ_RS04645 at 867.090 kb on - strand, within MMJJ_RS04645 at 867.151 kb on + strand, within MMJJ_RS04645 at 867.223 kb on - strand, within MMJJ_RS04645 at 867.223 kb on - strand, within MMJJ_RS04645 at 867.223 kb on - strand, within MMJJ_RS04645 at 867.223 kb on - strand, within MMJJ_RS04645 at 867.224 kb on + strand, within MMJJ_RS04645 at 867.224 kb on + strand, within MMJJ_RS04645 at 867.224 kb on + strand, within MMJJ_RS04645 at 867.224 kb on + strand, within MMJJ_RS04645 at 867.224 kb on + strand, within MMJJ_RS04645 at 867.224 kb on + strand, within MMJJ_RS04645 at 867.224 kb on + strand, within MMJJ_RS04645 at 867.224 kb on + strand, within MMJJ_RS04645 at 867.224 kb on + strand, within MMJJ_RS04645 at 867.224 kb on + strand, within MMJJ_RS04645 at 867.224 kb on + strand, within MMJJ_RS04645 at 867.224 kb on + strand, within MMJJ_RS04645 at 867.225 kb on - strand, within MMJJ_RS04645 at 867.286 kb on + strand, within MMJJ_RS04645 at 867.297 kb on - strand, within MMJJ_RS04645 at 867.297 kb on - strand, within MMJJ_RS04645 at 867.307 kb on - strand, within MMJJ_RS04645 at 867.308 kb on + strand, within MMJJ_RS04645 at 867.379 kb on + strand, within MMJJ_RS04645 at 867.400 kb on - strand at 867.401 kb on + strand at 867.425 kb on - strand at 867.460 kb on + strand at 867.543 kb on - strand at 867.567 kb on - strand at 867.567 kb on - strand at 867.567 kb on - strand at 867.567 kb on - strand at 867.567 kb on - strand at 867.568 kb on + strand at 867.571 kb on - strand at 867.571 kb on - strand at 867.571 kb on - strand at 867.571 kb on - strand at 867.572 kb on + strand at 867.572 kb on + strand at 867.643 kb on - strand at 867.643 kb on - strand at 867.646 kb on + strand at 867.647 kb on - strand at 867.649 kb on - strand at 867.671 kb on - strand at 867.714 kb on - strand at 867.715 kb on + strand at 867.715 kb on + strand at 867.715 kb on + strand at 867.715 kb on + strand at 867.715 kb on + strand at 867.734 kb on - strand at 867.735 kb on + strand at 867.797 kb on - strand at 867.798 kb on + strand at 867.802 kb on - strand at 867.802 kb on - strand at 867.802 kb on - strand at 867.803 kb on + strand at 867.805 kb on - strand at 867.805 kb on - strand at 867.808 kb on - strand at 867.812 kb on + strand at 867.812 kb on + strand at 867.812 kb on + strand at 867.816 kb on - strand at 867.816 kb on - strand at 867.817 kb on + strand at 867.817 kb on + strand at 867.817 kb on + strand at 867.817 kb on + strand at 867.817 kb on + strand at 867.818 kb on - strand at 867.819 kb on + strand at 867.819 kb on + strand at 867.819 kb on + strand at 867.821 kb on - strand at 867.821 kb on - strand at 867.821 kb on - strand at 867.826 kb on + strand at 867.835 kb on + strand at 867.836 kb on - strand at 867.836 kb on - strand at 867.836 kb on - strand at 867.836 kb on - strand at 867.836 kb on - strand at 867.836 kb on - strand at 867.836 kb on - strand at 867.836 kb on - strand at 867.837 kb on + strand at 867.837 kb on + strand at 867.837 kb on + strand at 867.837 kb on + strand at 867.837 kb on + strand at 867.837 kb on + strand at 867.840 kb on - strand at 867.840 kb on - strand at 867.841 kb on + strand at 867.841 kb on + strand at 867.841 kb on + strand at 867.841 kb on + strand at 867.841 kb on + strand at 867.841 kb on + strand at 867.843 kb on - strand at 867.843 kb on - strand at 867.843 kb on - strand at 867.843 kb on - strand at 867.843 kb on - strand at 867.843 kb on - strand at 867.844 kb on + strand at 867.844 kb on + strand at 867.844 kb on + strand at 867.844 kb on + strand at 867.845 kb on - strand at 867.845 kb on - strand at 867.846 kb on + strand at 867.897 kb on + strand at 868.151 kb on + strand, within MMJJ_RS04650 at 868.240 kb on - strand, within MMJJ_RS04650 at 868.291 kb on - strand, within MMJJ_RS04650 at 868.292 kb on + strand, within MMJJ_RS04650 at 868.292 kb on + strand, within MMJJ_RS04650 at 868.346 kb on - strand at 868.347 kb on + strand, within MMJJ_RS04650 at 868.372 kb on + strand, within MMJJ_RS04650 at 868.378 kb on - strand, within MMJJ_RS04650 at 868.379 kb on + strand, within MMJJ_RS04650 at 868.404 kb on - strand at 868.418 kb on - strand, within MMJJ_RS04650 at 868.420 kb on - strand, within MMJJ_RS04650 at 868.421 kb on + strand, within MMJJ_RS04650 at 868.421 kb on + strand, within MMJJ_RS04650 at 868.487 kb on + strand, within MMJJ_RS04650 at 868.512 kb on + strand, within MMJJ_RS04650 at 868.517 kb on + strand, within MMJJ_RS04650 at 868.538 kb on + strand, within MMJJ_RS04650 at 868.613 kb on + strand, within MMJJ_RS04650 at 868.617 kb on - strand, within MMJJ_RS04650 at 868.646 kb on + strand, within MMJJ_RS04650 at 868.671 kb on - strand, within MMJJ_RS04650 at 868.776 kb on - strand at 868.798 kb on - strand at 868.798 kb on - strand at 868.800 kb on - strand at 868.875 kb on - strand at 868.960 kb on - strand, within MMJJ_RS04655 at 869.003 kb on - strand, within MMJJ_RS04655 at 869.003 kb on - strand, within MMJJ_RS04655 at 869.050 kb on + strand at 869.165 kb on + strand, within MMJJ_RS04655 at 869.165 kb on + strand, within MMJJ_RS04655 at 869.173 kb on - strand, within MMJJ_RS04655 at 869.225 kb on + strand, within MMJJ_RS04655 at 869.347 kb on - strand, within MMJJ_RS04655 at 869.348 kb on + strand, within MMJJ_RS04655 at 869.349 kb on - strand at 869.349 kb on - strand, within MMJJ_RS04655 at 869.351 kb on - strand, within MMJJ_RS04655 at 869.451 kb on - strand at 869.480 kb on + strand, within MMJJ_RS04655 at 869.545 kb on - strand at 869.551 kb on - strand, within MMJJ_RS04655 at 869.618 kb on + strand, within MMJJ_RS04655 at 869.653 kb on - strand, within MMJJ_RS04655 at 869.653 kb on - strand, within MMJJ_RS04655 at 869.690 kb on + strand, within MMJJ_RS04655
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 2g/L casaminos remove 866,909 - MMJJ_RS04645 0.64 -0.1 866,910 + MMJJ_RS04645 0.64 -0.1 866,910 + MMJJ_RS04645 0.64 +0.1 866,991 - MMJJ_RS04645 0.69 +0.2 867,026 - MMJJ_RS04645 0.70 +0.3 867,067 - MMJJ_RS04645 0.73 +0.4 867,067 - MMJJ_RS04645 0.73 -1.1 867,068 + MMJJ_RS04645 0.73 +1.0 867,090 - MMJJ_RS04645 0.74 +0.2 867,151 + MMJJ_RS04645 0.77 -0.7 867,223 - MMJJ_RS04645 0.81 -0.1 867,223 - MMJJ_RS04645 0.81 +0.4 867,223 - MMJJ_RS04645 0.81 +0.2 867,223 - MMJJ_RS04645 0.81 +1.4 867,224 + MMJJ_RS04645 0.81 -0.4 867,224 + MMJJ_RS04645 0.81 -1.6 867,224 + MMJJ_RS04645 0.81 +0.1 867,224 + MMJJ_RS04645 0.81 -1.1 867,224 + MMJJ_RS04645 0.81 -0.2 867,224 + MMJJ_RS04645 0.81 +0.3 867,224 + MMJJ_RS04645 0.81 -1.3 867,224 + MMJJ_RS04645 0.81 +0.7 867,224 + MMJJ_RS04645 0.81 +0.2 867,224 + MMJJ_RS04645 0.81 -1.7 867,224 + MMJJ_RS04645 0.81 -0.6 867,224 + MMJJ_RS04645 0.81 +0.0 867,225 - MMJJ_RS04645 0.81 +0.4 867,286 + MMJJ_RS04645 0.84 +0.4 867,297 - MMJJ_RS04645 0.85 +0.2 867,297 - MMJJ_RS04645 0.85 +0.6 867,307 - MMJJ_RS04645 0.85 +0.2 867,308 + MMJJ_RS04645 0.85 +0.6 867,379 + MMJJ_RS04645 0.89 +0.6 867,400 - +0.4 867,401 + -0.1 867,425 - -0.0 867,460 + -0.2 867,543 - +0.1 867,567 - +1.0 867,567 - +0.6 867,567 - +0.5 867,567 - +0.6 867,567 - +1.0 867,568 + -0.2 867,571 - +0.3 867,571 - +0.9 867,571 - +0.6 867,571 - +0.6 867,572 + +0.7 867,572 + +0.5 867,643 - +0.1 867,643 - +0.7 867,646 + +0.1 867,647 - +0.5 867,649 - -0.1 867,671 - -0.3 867,714 - -1.5 867,715 + -0.7 867,715 + -1.2 867,715 + +0.8 867,715 + -2.1 867,715 + -0.2 867,734 - -1.3 867,735 + -0.4 867,797 - +0.8 867,798 + +0.8 867,802 - +1.1 867,802 - +0.5 867,802 - +0.5 867,803 + +0.7 867,805 - +0.3 867,805 - +0.6 867,808 - -0.9 867,812 + -0.1 867,812 + +0.4 867,812 + -1.2 867,816 - +0.6 867,816 - +0.3 867,817 + -2.0 867,817 + +0.3 867,817 + +0.8 867,817 + -0.5 867,817 + -1.0 867,818 - +0.4 867,819 + -0.5 867,819 + -0.1 867,819 + -0.0 867,821 - -2.9 867,821 - +0.2 867,821 - -0.1 867,826 + +0.3 867,835 + +0.6 867,836 - -0.9 867,836 - +0.3 867,836 - -0.6 867,836 - -0.8 867,836 - -1.2 867,836 - -3.6 867,836 - +1.5 867,836 - -0.1 867,837 + +0.6 867,837 + +0.3 867,837 + -1.2 867,837 + +0.7 867,837 + -0.4 867,837 + -0.2 867,840 - -0.6 867,840 - -1.7 867,841 + -0.0 867,841 + -0.7 867,841 + -1.4 867,841 + +1.1 867,841 + -0.9 867,841 + -0.5 867,843 - +0.7 867,843 - -0.4 867,843 - -0.2 867,843 - -0.4 867,843 - -3.1 867,843 - +1.2 867,844 + +0.3 867,844 + +0.1 867,844 + -1.4 867,844 + +0.3 867,845 - -0.8 867,845 - -1.9 867,846 + -0.8 867,897 + -3.4 868,151 + MMJJ_RS04650 0.29 -2.1 868,240 - MMJJ_RS04650 0.39 -1.4 868,291 - MMJJ_RS04650 0.44 -3.2 868,292 + MMJJ_RS04650 0.45 -3.0 868,292 + MMJJ_RS04650 0.45 -2.9 868,346 - -1.1 868,347 + MMJJ_RS04650 0.51 -0.4 868,372 + MMJJ_RS04650 0.54 -0.9 868,378 - MMJJ_RS04650 0.54 +0.2 868,379 + MMJJ_RS04650 0.54 -0.4 868,404 - -1.7 868,418 - MMJJ_RS04650 0.59 -1.9 868,420 - MMJJ_RS04650 0.59 -3.1 868,421 + MMJJ_RS04650 0.59 -2.3 868,421 + MMJJ_RS04650 0.59 -2.7 868,487 + MMJJ_RS04650 0.67 -1.9 868,512 + MMJJ_RS04650 0.70 -1.7 868,517 + MMJJ_RS04650 0.70 -1.2 868,538 + MMJJ_RS04650 0.73 -2.8 868,613 + MMJJ_RS04650 0.81 -1.7 868,617 - MMJJ_RS04650 0.82 -2.3 868,646 + MMJJ_RS04650 0.85 -3.6 868,671 - MMJJ_RS04650 0.88 -1.1 868,776 - -2.4 868,798 - -3.6 868,798 - -3.5 868,800 - -1.9 868,875 - -1.7 868,960 - MMJJ_RS04655 0.12 -2.9 869,003 - MMJJ_RS04655 0.15 -1.8 869,003 - MMJJ_RS04655 0.15 -0.3 869,050 + -1.9 869,165 + MMJJ_RS04655 0.26 -1.7 869,165 + MMJJ_RS04655 0.26 -2.6 869,173 - MMJJ_RS04655 0.27 -2.9 869,225 + MMJJ_RS04655 0.31 -3.7 869,347 - MMJJ_RS04655 0.39 -1.3 869,348 + MMJJ_RS04655 0.39 -1.6 869,349 - -0.1 869,349 - MMJJ_RS04655 0.40 -2.7 869,351 - MMJJ_RS04655 0.40 +0.7 869,451 - -1.7 869,480 + MMJJ_RS04655 0.49 -3.1 869,545 - -1.9 869,551 - MMJJ_RS04655 0.54 -1.6 869,618 + MMJJ_RS04655 0.59 -2.3 869,653 - MMJJ_RS04655 0.61 -2.4 869,653 - MMJJ_RS04655 0.61 -1.1 869,690 + MMJJ_RS04655 0.64 -1.9
Or see this region's nucleotide sequence