Experiment: H2 N-free with 2g/L casaminos
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS04605 and MMJJ_RS04610 are separated by 229 nucleotides MMJJ_RS04610 and MMJJ_RS04615 are separated by 236 nucleotides MMJJ_RS04615 and MMJJ_RS04620 are separated by 49 nucleotides
MMJJ_RS04605: MMJJ_RS04605 - 30S ribosomal protein S19e, at 857,708 to 858,133
_RS04605
MMJJ_RS04610: MMJJ_RS04610 - ribosome assembly RNA-binding protein YhbY, at 858,363 to 858,746
_RS04610
MMJJ_RS04615: MMJJ_RS04615 - methanogenesis marker 3 protein, at 858,983 to 860,488
_RS04615
MMJJ_RS04620: MMJJ_RS04620 - homocitrate synthase family protein, at 860,538 to 861,698
_RS04620
Position (kb) 858
859
860
861 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 858.136 kb on + strand at 858.260 kb on - strand at 858.276 kb on + strand at 858.299 kb on - strand at 858.300 kb on + strand at 858.303 kb on + strand at 858.304 kb on - strand at 858.305 kb on + strand at 858.362 kb on + strand at 858.415 kb on - strand, within MMJJ_RS04610 at 858.593 kb on - strand, within MMJJ_RS04610 at 858.602 kb on - strand, within MMJJ_RS04610 at 858.603 kb on + strand, within MMJJ_RS04610 at 858.610 kb on + strand, within MMJJ_RS04610 at 858.631 kb on + strand, within MMJJ_RS04610 at 858.631 kb on + strand, within MMJJ_RS04610 at 858.696 kb on - strand, within MMJJ_RS04610 at 858.697 kb on + strand, within MMJJ_RS04610 at 858.697 kb on + strand, within MMJJ_RS04610 at 858.700 kb on + strand, within MMJJ_RS04610 at 858.707 kb on - strand, within MMJJ_RS04610 at 858.720 kb on - strand at 858.726 kb on - strand at 858.746 kb on - strand at 858.762 kb on - strand at 858.763 kb on + strand at 858.766 kb on - strand at 858.766 kb on - strand at 858.766 kb on - strand at 858.766 kb on - strand at 858.766 kb on - strand at 858.766 kb on - strand at 858.766 kb on - strand at 858.766 kb on - strand at 858.766 kb on - strand at 858.766 kb on - strand at 858.766 kb on - strand at 858.766 kb on - strand at 858.766 kb on - strand at 858.766 kb on - strand at 858.766 kb on - strand at 858.767 kb on + strand at 858.767 kb on + strand at 858.767 kb on + strand at 858.767 kb on + strand at 858.767 kb on + strand at 858.767 kb on + strand at 858.767 kb on + strand at 858.767 kb on + strand at 858.767 kb on + strand at 858.767 kb on + strand at 858.767 kb on + strand at 858.767 kb on + strand at 858.767 kb on + strand at 858.772 kb on - strand at 858.773 kb on + strand at 858.773 kb on + strand at 858.774 kb on - strand at 858.775 kb on + strand at 858.775 kb on + strand at 858.783 kb on + strand at 858.793 kb on + strand at 858.793 kb on + strand at 858.805 kb on + strand at 858.827 kb on + strand at 858.827 kb on + strand at 858.833 kb on - strand at 858.838 kb on - strand at 858.838 kb on - strand at 858.839 kb on + strand at 858.855 kb on - strand at 858.856 kb on + strand at 858.860 kb on + strand at 858.860 kb on + strand at 858.864 kb on - strand at 858.869 kb on - strand at 858.869 kb on - strand at 858.869 kb on - strand at 858.870 kb on + strand at 858.870 kb on + strand at 858.871 kb on - strand at 858.871 kb on - strand at 858.871 kb on - strand at 858.871 kb on - strand at 858.871 kb on - strand at 858.871 kb on - strand at 858.871 kb on - strand at 858.871 kb on - strand at 858.871 kb on - strand at 858.871 kb on - strand at 858.871 kb on - strand at 858.872 kb on + strand at 858.872 kb on + strand at 858.872 kb on + strand at 858.872 kb on + strand at 858.872 kb on + strand at 858.872 kb on + strand at 858.872 kb on + strand at 858.872 kb on + strand at 858.872 kb on + strand at 858.872 kb on + strand at 858.873 kb on - strand at 858.873 kb on - strand at 858.873 kb on - strand at 858.873 kb on - strand at 858.873 kb on - strand at 858.873 kb on - strand at 858.873 kb on - strand at 858.873 kb on - strand at 858.873 kb on - strand at 858.873 kb on - strand at 858.873 kb on - strand at 858.874 kb on + strand at 858.874 kb on + strand at 858.874 kb on + strand at 858.874 kb on + strand at 858.875 kb on - strand at 858.875 kb on - strand at 858.875 kb on - strand at 858.875 kb on - strand at 858.875 kb on - strand at 858.876 kb on + strand at 858.876 kb on + strand at 858.876 kb on + strand at 858.876 kb on + strand at 858.880 kb on - strand at 858.880 kb on - strand at 858.881 kb on + strand at 858.881 kb on + strand at 858.881 kb on + strand at 858.881 kb on + strand at 858.886 kb on - strand at 858.887 kb on + strand at 858.894 kb on - strand at 858.894 kb on - strand at 858.894 kb on - strand at 858.895 kb on + strand at 858.895 kb on + strand at 858.895 kb on + strand at 858.895 kb on + strand at 858.897 kb on - strand at 858.898 kb on + strand at 858.898 kb on + strand at 858.898 kb on + strand at 858.898 kb on + strand at 858.898 kb on + strand at 858.903 kb on - strand at 858.904 kb on + strand at 858.904 kb on + strand at 858.907 kb on - strand at 858.907 kb on - strand at 858.908 kb on + strand at 858.908 kb on + strand at 858.911 kb on - strand at 858.911 kb on - strand at 858.912 kb on + strand at 858.913 kb on - strand at 858.914 kb on + strand at 858.914 kb on + strand at 858.918 kb on + strand at 858.918 kb on + strand at 858.919 kb on - strand at 858.919 kb on - strand at 858.955 kb on - strand at 858.955 kb on - strand at 858.956 kb on + strand at 858.991 kb on + strand at 859.036 kb on + strand at 859.074 kb on - strand at 859.074 kb on - strand at 859.099 kb on + strand at 859.477 kb on + strand, within MMJJ_RS04615 at 859.784 kb on - strand, within MMJJ_RS04615 at 859.789 kb on + strand, within MMJJ_RS04615 at 859.789 kb on + strand, within MMJJ_RS04615 at 859.789 kb on + strand, within MMJJ_RS04615 at 859.789 kb on + strand, within MMJJ_RS04615 at 860.030 kb on - strand, within MMJJ_RS04615 at 860.065 kb on - strand, within MMJJ_RS04615 at 860.129 kb on + strand, within MMJJ_RS04615 at 860.138 kb on - strand at 860.185 kb on + strand, within MMJJ_RS04615 at 860.212 kb on + strand, within MMJJ_RS04615 at 860.461 kb on + strand at 860.462 kb on - strand at 860.462 kb on - strand at 860.463 kb on + strand at 860.463 kb on + strand at 860.466 kb on - strand at 860.467 kb on + strand at 860.467 kb on + strand at 860.467 kb on + strand at 860.467 kb on + strand at 860.467 kb on + strand at 860.467 kb on + strand at 860.467 kb on + strand at 860.514 kb on - strand at 860.515 kb on + strand at 860.519 kb on - strand at 860.521 kb on - strand at 860.521 kb on - strand at 860.603 kb on - strand at 860.856 kb on + strand, within MMJJ_RS04620 at 860.897 kb on + strand, within MMJJ_RS04620 at 860.901 kb on + strand, within MMJJ_RS04620 at 861.074 kb on - strand, within MMJJ_RS04620 at 861.112 kb on - strand at 861.113 kb on + strand at 861.124 kb on - strand, within MMJJ_RS04620
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 2g/L casaminos remove 858,136 + -2.4 858,260 - -2.1 858,276 + -1.1 858,299 - +0.4 858,300 + -2.4 858,303 + -0.8 858,304 - -2.1 858,305 + -0.5 858,362 + +0.3 858,415 - MMJJ_RS04610 0.14 +0.8 858,593 - MMJJ_RS04610 0.60 +0.2 858,602 - MMJJ_RS04610 0.62 -0.2 858,603 + MMJJ_RS04610 0.62 +0.2 858,610 + MMJJ_RS04610 0.64 -0.4 858,631 + MMJJ_RS04610 0.70 -0.7 858,631 + MMJJ_RS04610 0.70 -1.4 858,696 - MMJJ_RS04610 0.87 -1.5 858,697 + MMJJ_RS04610 0.87 -0.7 858,697 + MMJJ_RS04610 0.87 +0.7 858,700 + MMJJ_RS04610 0.88 -0.6 858,707 - MMJJ_RS04610 0.90 -0.6 858,720 - +0.4 858,726 - +0.1 858,746 - -1.0 858,762 - -2.1 858,763 + -1.0 858,766 - -0.1 858,766 - +1.1 858,766 - +1.6 858,766 - -0.6 858,766 - -0.4 858,766 - -0.5 858,766 - -0.1 858,766 - +0.9 858,766 - +0.4 858,766 - +0.3 858,766 - +0.8 858,766 - -0.4 858,766 - -0.4 858,766 - -0.1 858,766 - +0.5 858,767 + +0.0 858,767 + -0.4 858,767 + +0.5 858,767 + -1.2 858,767 + +1.0 858,767 + +0.5 858,767 + -0.0 858,767 + +0.4 858,767 + +0.5 858,767 + -0.3 858,767 + -1.1 858,767 + +0.7 858,767 + -0.1 858,772 - -1.4 858,773 + -0.0 858,773 + -0.2 858,774 - -1.9 858,775 + +0.9 858,775 + -0.4 858,783 + -0.1 858,793 + -0.9 858,793 + +0.1 858,805 + +0.5 858,827 + -0.2 858,827 + -1.1 858,833 - -1.4 858,838 - -1.9 858,838 - -0.6 858,839 + +0.6 858,855 - -0.8 858,856 + -1.3 858,860 + +1.1 858,860 + -1.9 858,864 - -0.7 858,869 - +0.0 858,869 - -0.2 858,869 - +0.4 858,870 + -0.9 858,870 + -2.0 858,871 - -0.8 858,871 - -1.2 858,871 - -0.5 858,871 - -0.4 858,871 - -0.0 858,871 - -0.1 858,871 - -0.7 858,871 - +0.1 858,871 - -0.5 858,871 - +0.7 858,871 - +1.1 858,872 + -0.4 858,872 + +0.7 858,872 + +0.3 858,872 + -0.5 858,872 + -2.4 858,872 + -1.1 858,872 + -0.3 858,872 + +0.4 858,872 + +0.3 858,872 + +1.0 858,873 - -3.1 858,873 - -1.3 858,873 - -1.1 858,873 - -0.6 858,873 - -0.5 858,873 - +0.7 858,873 - -0.7 858,873 - -0.7 858,873 - -1.4 858,873 - +0.1 858,873 - +0.6 858,874 + -0.2 858,874 + -0.2 858,874 + +1.4 858,874 + -1.8 858,875 - +0.4 858,875 - +0.6 858,875 - -2.0 858,875 - -1.4 858,875 - -1.0 858,876 + +0.1 858,876 + +0.4 858,876 + +0.9 858,876 + +0.9 858,880 - -0.6 858,880 - -1.5 858,881 + -1.1 858,881 + -0.4 858,881 + +0.6 858,881 + +0.5 858,886 - -0.0 858,887 + +0.7 858,894 - -2.2 858,894 - -1.0 858,894 - +0.0 858,895 + -0.0 858,895 + -1.0 858,895 + -0.4 858,895 + -0.1 858,897 - +0.2 858,898 + -0.6 858,898 + -1.0 858,898 + +0.2 858,898 + +0.2 858,898 + +0.5 858,903 - -3.3 858,904 + -1.7 858,904 + -0.8 858,907 - -0.9 858,907 - -1.5 858,908 + -0.1 858,908 + -0.6 858,911 - +0.4 858,911 - -1.6 858,912 + +0.1 858,913 - -0.9 858,914 + -0.3 858,914 + -0.2 858,918 + +0.4 858,918 + -0.2 858,919 - -0.7 858,919 - -0.7 858,955 - -2.9 858,955 - -4.5 858,956 + -2.1 858,991 + -2.7 859,036 + -1.9 859,074 - -2.1 859,074 - -2.4 859,099 + -2.6 859,477 + MMJJ_RS04615 0.33 -1.9 859,784 - MMJJ_RS04615 0.53 -2.1 859,789 + MMJJ_RS04615 0.54 -3.0 859,789 + MMJJ_RS04615 0.54 -3.1 859,789 + MMJJ_RS04615 0.54 -1.1 859,789 + MMJJ_RS04615 0.54 -2.4 860,030 - MMJJ_RS04615 0.70 +0.7 860,065 - MMJJ_RS04615 0.72 -3.2 860,129 + MMJJ_RS04615 0.76 -2.8 860,138 - -1.1 860,185 + MMJJ_RS04615 0.80 -2.6 860,212 + MMJJ_RS04615 0.82 -3.3 860,461 + -1.4 860,462 - -1.7 860,462 - -2.4 860,463 + -1.7 860,463 + -0.2 860,466 - -1.4 860,467 + +0.5 860,467 + -0.3 860,467 + +0.6 860,467 + -0.7 860,467 + +0.3 860,467 + -2.1 860,467 + -0.1 860,514 - -2.1 860,515 + -1.7 860,519 - -1.1 860,521 - -0.1 860,521 - -1.7 860,603 - -3.6 860,856 + MMJJ_RS04620 0.27 -2.1 860,897 + MMJJ_RS04620 0.31 -2.1 860,901 + MMJJ_RS04620 0.31 +0.5 861,074 - MMJJ_RS04620 0.46 -1.9 861,112 - -0.1 861,113 + -0.1 861,124 - MMJJ_RS04620 0.50 -2.1
Or see this region's nucleotide sequence