Experiment: H2 N-free with 2g/L casaminos
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS03265 and MMJJ_RS03270 are separated by 429 nucleotides MMJJ_RS03270 and MMJJ_RS03275 are separated by 76 nucleotides MMJJ_RS03275 and MMJJ_RS03280 are separated by 181 nucleotides
MMJJ_RS03265: MMJJ_RS03265 - S-layer protein, at 615,714 to 617,330
_RS03265
MMJJ_RS03270: MMJJ_RS03270 - ribosome biogenesis/translation initiation ATPase RLI, at 617,760 to 619,532
_RS03270
MMJJ_RS03275: MMJJ_RS03275 - homoaconitase small subunit, at 619,609 to 620,094
_RS03275
MMJJ_RS03280: MMJJ_RS03280 - DNA-directed DNA polymerase, at 620,276 to 622,630
_RS03280
Position (kb) 617
618
619
620 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 616.813 kb on + strand, within MMJJ_RS03265 at 617.568 kb on + strand at 617.568 kb on + strand at 617.586 kb on + strand at 617.668 kb on + strand at 617.671 kb on + strand at 617.678 kb on - strand at 617.678 kb on - strand at 617.678 kb on - strand at 617.679 kb on + strand at 617.679 kb on + strand at 617.683 kb on - strand at 617.683 kb on - strand at 617.752 kb on - strand at 617.752 kb on - strand at 617.761 kb on - strand at 617.773 kb on - strand at 618.109 kb on + strand, within MMJJ_RS03270 at 618.505 kb on - strand at 618.597 kb on - strand, within MMJJ_RS03270 at 618.651 kb on - strand, within MMJJ_RS03270 at 618.685 kb on + strand, within MMJJ_RS03270 at 619.463 kb on + strand at 619.502 kb on - strand at 619.566 kb on - strand at 619.571 kb on - strand at 619.660 kb on + strand, within MMJJ_RS03275 at 620.068 kb on - strand at 620.153 kb on - strand at 620.194 kb on + strand at 620.195 kb on - strand at 620.196 kb on + strand at 620.206 kb on + strand at 620.209 kb on + strand at 620.230 kb on - strand at 620.242 kb on - strand at 620.242 kb on - strand at 620.242 kb on - strand at 620.242 kb on - strand at 620.243 kb on + strand at 620.243 kb on + strand at 620.322 kb on - strand at 620.324 kb on - strand at 620.324 kb on - strand at 620.324 kb on - strand at 620.325 kb on + strand at 620.325 kb on + strand at 620.325 kb on + strand at 620.325 kb on + strand at 620.325 kb on + strand at 620.328 kb on - strand at 620.328 kb on - strand at 620.328 kb on - strand at 620.328 kb on - strand at 620.328 kb on - strand at 620.328 kb on - strand at 620.329 kb on + strand at 620.329 kb on + strand at 620.329 kb on + strand at 620.329 kb on + strand at 620.329 kb on + strand at 620.331 kb on + strand at 620.334 kb on - strand at 620.334 kb on - strand at 620.335 kb on + strand at 620.335 kb on + strand at 620.337 kb on - strand at 620.338 kb on + strand at 620.341 kb on - strand at 620.342 kb on + strand at 620.349 kb on - strand at 620.350 kb on + strand at 620.373 kb on + strand at 620.382 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 2g/L casaminos remove 616,813 + MMJJ_RS03265 0.68 +0.1 617,568 + -0.1 617,568 + -0.0 617,586 + +1.0 617,668 + +0.5 617,671 + +0.3 617,678 - +0.1 617,678 - -0.3 617,678 - -0.8 617,679 + +0.3 617,679 + +0.8 617,683 - +0.0 617,683 - +1.2 617,752 - -1.4 617,752 - +0.2 617,761 - -1.1 617,773 - -1.0 618,109 + MMJJ_RS03270 0.20 -3.0 618,505 - -0.7 618,597 - MMJJ_RS03270 0.47 -3.0 618,651 - MMJJ_RS03270 0.50 -3.2 618,685 + MMJJ_RS03270 0.52 -3.8 619,463 + -3.3 619,502 - -0.1 619,566 - -1.9 619,571 - +0.5 619,660 + MMJJ_RS03275 0.10 -2.3 620,068 - -1.5 620,153 - -0.7 620,194 + -1.5 620,195 - -1.9 620,196 + -1.7 620,206 + +1.7 620,209 + -0.6 620,230 - -0.2 620,242 - +0.4 620,242 - -0.5 620,242 - -0.3 620,242 - +0.2 620,243 + -0.5 620,243 + -0.3 620,322 - +0.0 620,324 - +0.2 620,324 - +0.3 620,324 - +0.7 620,325 + +0.8 620,325 + -1.5 620,325 + +0.9 620,325 + +0.6 620,325 + +0.5 620,328 - -0.3 620,328 - +0.0 620,328 - -0.8 620,328 - +0.1 620,328 - -1.1 620,328 - +0.4 620,329 + -0.4 620,329 + +0.2 620,329 + -0.9 620,329 + -2.1 620,329 + -0.3 620,331 + +1.1 620,334 - +0.9 620,334 - +0.5 620,335 + +0.1 620,335 + +0.6 620,337 - -0.3 620,338 + -0.8 620,341 - +0.4 620,342 + +1.2 620,349 - -1.9 620,350 + -0.9 620,373 + +1.2 620,382 - +0.4
Or see this region's nucleotide sequence