Experiment: H2 N-free with 2g/L casaminos
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS03120 and MMJJ_RS03125 are separated by 131 nucleotides MMJJ_RS03125 and MMJJ_RS03130 are separated by 21 nucleotides
MMJJ_RS03120: MMJJ_RS03120 - ribose-phosphate diphosphokinase, at 589,229 to 590,092
_RS03120
MMJJ_RS03125: MMJJ_RS03125 - GMC oxidoreductase, at 590,224 to 591,384
_RS03125
MMJJ_RS03130: MMJJ_RS03130 - DUF1611 domain-containing protein, at 591,406 to 592,326
_RS03130
Position (kb) 590
591
592 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 589.226 kb on - strand at 589.227 kb on + strand at 589.230 kb on - strand at 589.241 kb on - strand at 589.244 kb on - strand at 589.244 kb on - strand at 589.244 kb on - strand at 589.244 kb on - strand at 589.245 kb on + strand at 589.272 kb on + strand at 589.485 kb on + strand at 589.485 kb on + strand at 589.844 kb on - strand, within MMJJ_RS03120 at 590.126 kb on - strand at 590.147 kb on - strand at 590.147 kb on - strand at 590.165 kb on - strand at 590.181 kb on - strand at 590.244 kb on + strand at 590.265 kb on - strand at 590.310 kb on + strand at 590.310 kb on + strand at 590.310 kb on + strand at 590.341 kb on - strand, within MMJJ_RS03125 at 590.468 kb on - strand, within MMJJ_RS03125 at 590.469 kb on + strand at 590.469 kb on + strand, within MMJJ_RS03125 at 590.470 kb on - strand, within MMJJ_RS03125 at 590.706 kb on + strand at 590.736 kb on + strand, within MMJJ_RS03125 at 590.763 kb on - strand, within MMJJ_RS03125 at 590.770 kb on - strand, within MMJJ_RS03125 at 590.834 kb on - strand, within MMJJ_RS03125 at 590.835 kb on + strand, within MMJJ_RS03125 at 590.838 kb on + strand, within MMJJ_RS03125 at 590.888 kb on - strand, within MMJJ_RS03125 at 591.283 kb on - strand at 591.342 kb on - strand at 591.439 kb on - strand at 591.439 kb on - strand at 591.440 kb on + strand at 591.482 kb on + strand at 591.580 kb on - strand, within MMJJ_RS03130 at 591.580 kb on - strand, within MMJJ_RS03130 at 591.760 kb on - strand, within MMJJ_RS03130 at 591.903 kb on - strand, within MMJJ_RS03130 at 591.985 kb on - strand, within MMJJ_RS03130 at 591.994 kb on + strand, within MMJJ_RS03130 at 592.022 kb on - strand, within MMJJ_RS03130 at 592.022 kb on - strand, within MMJJ_RS03130 at 592.022 kb on - strand, within MMJJ_RS03130 at 592.023 kb on + strand, within MMJJ_RS03130 at 592.030 kb on - strand, within MMJJ_RS03130 at 592.031 kb on + strand, within MMJJ_RS03130 at 592.064 kb on - strand, within MMJJ_RS03130 at 592.065 kb on + strand, within MMJJ_RS03130 at 592.067 kb on - strand, within MMJJ_RS03130 at 592.067 kb on - strand, within MMJJ_RS03130 at 592.072 kb on - strand, within MMJJ_RS03130 at 592.079 kb on - strand, within MMJJ_RS03130 at 592.079 kb on - strand, within MMJJ_RS03130 at 592.142 kb on - strand, within MMJJ_RS03130 at 592.142 kb on - strand, within MMJJ_RS03130 at 592.142 kb on - strand, within MMJJ_RS03130 at 592.143 kb on + strand, within MMJJ_RS03130 at 592.146 kb on - strand, within MMJJ_RS03130 at 592.147 kb on + strand, within MMJJ_RS03130 at 592.150 kb on + strand, within MMJJ_RS03130 at 592.150 kb on + strand, within MMJJ_RS03130 at 592.162 kb on + strand, within MMJJ_RS03130 at 592.213 kb on - strand, within MMJJ_RS03130 at 592.214 kb on + strand, within MMJJ_RS03130 at 592.214 kb on + strand, within MMJJ_RS03130 at 592.230 kb on - strand, within MMJJ_RS03130 at 592.230 kb on - strand, within MMJJ_RS03130 at 592.231 kb on + strand, within MMJJ_RS03130 at 592.231 kb on + strand, within MMJJ_RS03130 at 592.241 kb on - strand at 592.255 kb on - strand at 592.255 kb on - strand at 592.255 kb on - strand at 592.256 kb on + strand at 592.256 kb on + strand at 592.326 kb on - strand at 592.327 kb on + strand at 592.329 kb on + strand at 592.365 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 2g/L casaminos remove 589,226 - -0.7 589,227 + -0.1 589,230 - -0.2 589,241 - +0.1 589,244 - +0.0 589,244 - +0.4 589,244 - -0.9 589,244 - -1.0 589,245 + +0.7 589,272 + -1.1 589,485 + -0.5 589,485 + -1.5 589,844 - MMJJ_RS03120 0.71 -1.4 590,126 - -1.9 590,147 - -1.1 590,147 - -0.8 590,165 - +0.1 590,181 - -0.8 590,244 + -2.3 590,265 - -0.9 590,310 + -0.7 590,310 + -1.7 590,310 + -1.9 590,341 - MMJJ_RS03125 0.10 -2.1 590,468 - MMJJ_RS03125 0.21 -1.7 590,469 + -1.5 590,469 + MMJJ_RS03125 0.21 -2.1 590,470 - MMJJ_RS03125 0.21 -2.9 590,706 + -1.1 590,736 + MMJJ_RS03125 0.44 -1.3 590,763 - MMJJ_RS03125 0.46 -1.7 590,770 - MMJJ_RS03125 0.47 -1.9 590,834 - MMJJ_RS03125 0.53 -2.3 590,835 + MMJJ_RS03125 0.53 -2.1 590,838 + MMJJ_RS03125 0.53 -1.1 590,888 - MMJJ_RS03125 0.57 -1.5 591,283 - -1.5 591,342 - -3.1 591,439 - -0.6 591,439 - +1.1 591,440 + +0.0 591,482 + -0.8 591,580 - MMJJ_RS03130 0.19 +0.4 591,580 - MMJJ_RS03130 0.19 -1.9 591,760 - MMJJ_RS03130 0.38 +0.4 591,903 - MMJJ_RS03130 0.54 -1.1 591,985 - MMJJ_RS03130 0.63 -2.5 591,994 + MMJJ_RS03130 0.64 -2.5 592,022 - MMJJ_RS03130 0.67 -0.9 592,022 - MMJJ_RS03130 0.67 -2.5 592,022 - MMJJ_RS03130 0.67 +0.1 592,023 + MMJJ_RS03130 0.67 +0.0 592,030 - MMJJ_RS03130 0.68 +0.2 592,031 + MMJJ_RS03130 0.68 -0.1 592,064 - MMJJ_RS03130 0.71 -0.1 592,065 + MMJJ_RS03130 0.72 -0.5 592,067 - MMJJ_RS03130 0.72 -2.8 592,067 - MMJJ_RS03130 0.72 -0.5 592,072 - MMJJ_RS03130 0.72 -2.6 592,079 - MMJJ_RS03130 0.73 -0.3 592,079 - MMJJ_RS03130 0.73 -1.0 592,142 - MMJJ_RS03130 0.80 -0.4 592,142 - MMJJ_RS03130 0.80 -0.7 592,142 - MMJJ_RS03130 0.80 -1.1 592,143 + MMJJ_RS03130 0.80 -0.3 592,146 - MMJJ_RS03130 0.80 -1.4 592,147 + MMJJ_RS03130 0.80 +0.0 592,150 + MMJJ_RS03130 0.81 -0.1 592,150 + MMJJ_RS03130 0.81 -2.5 592,162 + MMJJ_RS03130 0.82 -0.7 592,213 - MMJJ_RS03130 0.88 -0.5 592,214 + MMJJ_RS03130 0.88 -0.7 592,214 + MMJJ_RS03130 0.88 -0.8 592,230 - MMJJ_RS03130 0.89 -0.0 592,230 - MMJJ_RS03130 0.89 -1.9 592,231 + MMJJ_RS03130 0.90 +0.1 592,231 + MMJJ_RS03130 0.90 -0.1 592,241 - +1.3 592,255 - +0.0 592,255 - -0.7 592,255 - -0.3 592,256 + +0.3 592,256 + -0.1 592,326 - -0.0 592,327 + -1.0 592,329 + -0.4 592,365 - -0.6
Or see this region's nucleotide sequence