Experiment: H2 N-free with 2g/L casaminos
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS02920 and MMJJ_RS02925 are separated by 1 nucleotides MMJJ_RS02925 and MMJJ_RS02930 are separated by 6 nucleotides MMJJ_RS02930 and MMJJ_RS02935 are separated by 31 nucleotides MMJJ_RS02935 and MMJJ_RS02940 are separated by 47 nucleotides MMJJ_RS02940 and MMJJ_RS02945 are separated by 155 nucleotides
MMJJ_RS02920: MMJJ_RS02920 - 30S ribosomal protein S27ae, at 547,476 to 547,679
_RS02920
MMJJ_RS02925: MMJJ_RS02925 - 30S ribosomal protein S24e, at 547,681 to 547,989
_RS02925
MMJJ_RS02930: MMJJ_RS02930 - DUF359 domain-containing protein, at 547,996 to 548,472
_RS02930
MMJJ_RS02935: MMJJ_RS02935 - DNA-directed RNA polymerase subunit E'', at 548,504 to 548,680
_RS02935
MMJJ_RS02940: MMJJ_RS02940 - DNA-directed RNA polymerase, at 548,728 to 549,291
_RS02940
MMJJ_RS02945: MMJJ_RS02945 - dihydroorotate dehydrogenase, at 549,447 to 550,361
_RS02945
Position (kb) 548
549 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 547.520 kb on + strand, within MMJJ_RS02920 at 547.566 kb on - strand, within MMJJ_RS02920 at 547.631 kb on + strand, within MMJJ_RS02920 at 547.633 kb on - strand, within MMJJ_RS02920 at 547.694 kb on + strand at 548.301 kb on - strand, within MMJJ_RS02930 at 548.468 kb on - strand at 548.483 kb on - strand at 548.484 kb on + strand at 548.484 kb on + strand at 548.484 kb on + strand at 548.484 kb on + strand at 548.485 kb on - strand at 548.486 kb on + strand at 548.486 kb on + strand at 548.500 kb on - strand at 548.505 kb on - strand at 548.506 kb on + strand at 548.506 kb on + strand at 548.506 kb on + strand at 548.534 kb on - strand, within MMJJ_RS02935 at 548.534 kb on - strand, within MMJJ_RS02935 at 548.535 kb on + strand, within MMJJ_RS02935 at 548.667 kb on + strand at 548.708 kb on - strand at 548.709 kb on + strand at 548.743 kb on - strand at 549.321 kb on - strand at 549.335 kb on - strand at 549.353 kb on - strand at 549.356 kb on - strand at 549.370 kb on - strand at 549.370 kb on - strand at 549.370 kb on - strand at 549.372 kb on - strand at 549.372 kb on - strand at 549.373 kb on + strand at 549.374 kb on - strand at 549.374 kb on - strand at 549.374 kb on - strand at 549.374 kb on - strand at 549.374 kb on - strand at 549.374 kb on - strand at 549.375 kb on + strand at 549.375 kb on + strand at 549.375 kb on + strand at 549.376 kb on - strand at 549.376 kb on - strand at 549.376 kb on - strand at 549.376 kb on - strand at 549.376 kb on - strand at 549.377 kb on + strand at 549.377 kb on + strand at 549.377 kb on + strand at 549.392 kb on + strand at 549.395 kb on - strand at 549.396 kb on + strand at 549.416 kb on + strand at 549.418 kb on + strand at 549.667 kb on + strand, within MMJJ_RS02945 at 549.673 kb on - strand, within MMJJ_RS02945
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 2g/L casaminos remove 547,520 + MMJJ_RS02920 0.22 -2.4 547,566 - MMJJ_RS02920 0.44 -2.9 547,631 + MMJJ_RS02920 0.76 -2.6 547,633 - MMJJ_RS02920 0.77 -2.5 547,694 + -2.3 548,301 - MMJJ_RS02930 0.64 -1.5 548,468 - -3.0 548,483 - +0.0 548,484 + -1.3 548,484 + -1.6 548,484 + -0.6 548,484 + -2.2 548,485 - -0.7 548,486 + +0.2 548,486 + +0.0 548,500 - +0.6 548,505 - -0.2 548,506 + -1.0 548,506 + -1.3 548,506 + -2.3 548,534 - MMJJ_RS02935 0.17 -1.2 548,534 - MMJJ_RS02935 0.17 -2.3 548,535 + MMJJ_RS02935 0.18 -2.9 548,667 + -1.1 548,708 - -1.5 548,709 + -0.7 548,743 - -0.9 549,321 - -1.4 549,335 - -0.6 549,353 - +0.1 549,356 - -0.9 549,370 - -1.8 549,370 - -2.7 549,370 - -1.7 549,372 - -1.8 549,372 - -1.7 549,373 + -1.1 549,374 - -1.1 549,374 - -2.0 549,374 - -2.2 549,374 - -1.1 549,374 - -0.7 549,374 - -0.5 549,375 + -2.7 549,375 + -1.5 549,375 + -1.9 549,376 - -3.5 549,376 - -0.4 549,376 - -1.8 549,376 - -0.6 549,376 - -2.2 549,377 + -0.6 549,377 + -2.2 549,377 + -2.6 549,392 + -0.7 549,395 - -0.8 549,396 + -0.1 549,416 + +1.0 549,418 + -0.4 549,667 + MMJJ_RS02945 0.24 -0.5 549,673 - MMJJ_RS02945 0.25 -2.9
Or see this region's nucleotide sequence