Experiment: H2 N-free with 2g/L casaminos
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS02045 and MMJJ_RS02050 are separated by 110 nucleotides MMJJ_RS02050 and MMJJ_RS02055 are separated by 132 nucleotides MMJJ_RS02055 and MMJJ_RS02060 are separated by 8 nucleotides MMJJ_RS02060 and MMJJ_RS02065 are separated by 73 nucleotides
MMJJ_RS02045: MMJJ_RS02045 - molybdenum cofactor guanylyltransferase, at 389,718 to 390,362
_RS02045
MMJJ_RS02050: MMJJ_RS02050 - peptidylprolyl isomerase, at 390,473 to 391,159
_RS02050
MMJJ_RS02055: MMJJ_RS02055 - GTP 3',8-cyclase MoaA, at 391,292 to 392,185
_RS02055
MMJJ_RS02060: MMJJ_RS02060 - cupin domain-containing protein, at 392,194 to 392,694
_RS02060
MMJJ_RS02065: MMJJ_RS02065 - S-layer protein, at 392,768 to 394,354
_RS02065
Position (kb) 391
392
393 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 390.362 kb on - strand at 390.362 kb on - strand at 390.400 kb on - strand at 390.400 kb on - strand at 390.400 kb on - strand at 390.401 kb on + strand at 390.401 kb on + strand at 390.401 kb on + strand at 390.401 kb on + strand at 390.402 kb on - strand at 390.404 kb on - strand at 390.404 kb on - strand at 390.405 kb on + strand at 390.405 kb on + strand at 390.405 kb on + strand at 390.413 kb on + strand at 390.417 kb on + strand at 390.418 kb on - strand at 390.418 kb on - strand at 390.430 kb on - strand at 390.449 kb on - strand at 390.449 kb on - strand at 390.450 kb on + strand at 390.493 kb on + strand at 390.504 kb on + strand at 390.513 kb on + strand at 390.590 kb on - strand, within MMJJ_RS02050 at 390.767 kb on + strand, within MMJJ_RS02050 at 390.825 kb on - strand, within MMJJ_RS02050 at 390.826 kb on + strand, within MMJJ_RS02050 at 390.847 kb on + strand, within MMJJ_RS02050 at 390.849 kb on - strand, within MMJJ_RS02050 at 390.850 kb on + strand, within MMJJ_RS02050 at 390.850 kb on + strand, within MMJJ_RS02050 at 390.939 kb on - strand, within MMJJ_RS02050 at 390.939 kb on - strand, within MMJJ_RS02050 at 390.984 kb on - strand, within MMJJ_RS02050 at 390.990 kb on - strand, within MMJJ_RS02050 at 390.990 kb on - strand, within MMJJ_RS02050 at 391.011 kb on - strand, within MMJJ_RS02050 at 391.011 kb on - strand, within MMJJ_RS02050 at 391.017 kb on - strand, within MMJJ_RS02050 at 391.042 kb on - strand, within MMJJ_RS02050 at 391.042 kb on - strand, within MMJJ_RS02050 at 391.042 kb on - strand, within MMJJ_RS02050 at 391.043 kb on + strand, within MMJJ_RS02050 at 391.043 kb on + strand, within MMJJ_RS02050 at 391.171 kb on - strand at 391.171 kb on - strand at 391.172 kb on + strand at 391.192 kb on - strand at 391.193 kb on + strand at 391.193 kb on + strand at 391.251 kb on + strand at 391.287 kb on - strand at 391.346 kb on + strand at 391.527 kb on + strand, within MMJJ_RS02055 at 391.527 kb on + strand, within MMJJ_RS02055 at 391.527 kb on + strand, within MMJJ_RS02055 at 391.527 kb on + strand, within MMJJ_RS02055 at 391.546 kb on + strand at 391.564 kb on - strand, within MMJJ_RS02055 at 391.578 kb on - strand, within MMJJ_RS02055 at 391.589 kb on - strand, within MMJJ_RS02055 at 391.590 kb on + strand, within MMJJ_RS02055 at 391.638 kb on - strand, within MMJJ_RS02055 at 391.642 kb on - strand, within MMJJ_RS02055 at 391.643 kb on + strand, within MMJJ_RS02055 at 391.728 kb on - strand, within MMJJ_RS02055 at 391.735 kb on + strand, within MMJJ_RS02055 at 391.735 kb on + strand, within MMJJ_RS02055 at 391.844 kb on + strand, within MMJJ_RS02055 at 391.863 kb on - strand, within MMJJ_RS02055 at 391.863 kb on - strand, within MMJJ_RS02055 at 391.878 kb on - strand, within MMJJ_RS02055 at 391.879 kb on + strand, within MMJJ_RS02055 at 391.879 kb on + strand, within MMJJ_RS02055 at 391.926 kb on - strand, within MMJJ_RS02055 at 391.927 kb on + strand, within MMJJ_RS02055 at 391.927 kb on + strand, within MMJJ_RS02055 at 391.927 kb on + strand, within MMJJ_RS02055 at 391.930 kb on - strand, within MMJJ_RS02055 at 391.961 kb on - strand, within MMJJ_RS02055 at 391.978 kb on + strand, within MMJJ_RS02055 at 392.037 kb on - strand, within MMJJ_RS02055 at 392.045 kb on + strand, within MMJJ_RS02055 at 392.045 kb on + strand, within MMJJ_RS02055 at 392.045 kb on + strand, within MMJJ_RS02055 at 392.057 kb on - strand, within MMJJ_RS02055 at 392.061 kb on - strand, within MMJJ_RS02055 at 392.061 kb on - strand, within MMJJ_RS02055 at 392.086 kb on - strand, within MMJJ_RS02055 at 392.128 kb on - strand at 392.129 kb on + strand at 392.129 kb on + strand at 392.192 kb on - strand at 392.209 kb on - strand at 392.217 kb on - strand at 392.254 kb on - strand, within MMJJ_RS02060 at 392.255 kb on + strand, within MMJJ_RS02060 at 392.270 kb on + strand, within MMJJ_RS02060 at 392.270 kb on + strand, within MMJJ_RS02060 at 392.303 kb on - strand, within MMJJ_RS02060 at 392.315 kb on + strand, within MMJJ_RS02060 at 392.315 kb on + strand, within MMJJ_RS02060 at 392.316 kb on - strand, within MMJJ_RS02060 at 392.317 kb on + strand, within MMJJ_RS02060 at 392.317 kb on + strand, within MMJJ_RS02060 at 392.338 kb on + strand, within MMJJ_RS02060 at 392.347 kb on - strand, within MMJJ_RS02060 at 392.358 kb on + strand, within MMJJ_RS02060 at 392.413 kb on - strand, within MMJJ_RS02060 at 392.416 kb on - strand, within MMJJ_RS02060 at 392.432 kb on - strand, within MMJJ_RS02060 at 392.433 kb on + strand, within MMJJ_RS02060 at 392.436 kb on - strand, within MMJJ_RS02060 at 392.436 kb on - strand, within MMJJ_RS02060 at 392.442 kb on - strand, within MMJJ_RS02060 at 392.443 kb on + strand, within MMJJ_RS02060 at 392.443 kb on + strand, within MMJJ_RS02060 at 392.464 kb on - strand, within MMJJ_RS02060 at 392.464 kb on - strand, within MMJJ_RS02060 at 392.466 kb on - strand, within MMJJ_RS02060 at 392.466 kb on - strand, within MMJJ_RS02060 at 392.466 kb on - strand, within MMJJ_RS02060 at 392.467 kb on + strand, within MMJJ_RS02060 at 392.467 kb on + strand, within MMJJ_RS02060 at 392.487 kb on - strand, within MMJJ_RS02060 at 392.487 kb on - strand, within MMJJ_RS02060 at 392.516 kb on - strand, within MMJJ_RS02060 at 392.516 kb on - strand, within MMJJ_RS02060 at 392.708 kb on - strand at 392.708 kb on - strand at 392.709 kb on + strand at 392.712 kb on + strand at 392.717 kb on + strand at 392.717 kb on + strand at 392.717 kb on + strand at 392.737 kb on - strand at 392.738 kb on + strand at 392.738 kb on + strand at 392.738 kb on + strand at 392.739 kb on - strand at 392.739 kb on - strand at 392.742 kb on - strand at 392.743 kb on + strand at 392.743 kb on + strand at 392.767 kb on - strand at 392.767 kb on - strand at 392.768 kb on + strand at 392.797 kb on - strand at 392.797 kb on - strand at 392.797 kb on - strand at 392.812 kb on - strand at 392.817 kb on - strand at 392.817 kb on - strand at 392.817 kb on - strand at 392.817 kb on - strand at 392.820 kb on - strand at 392.880 kb on - strand at 392.881 kb on + strand at 392.892 kb on - strand at 392.896 kb on + strand at 392.918 kb on - strand at 392.925 kb on - strand at 393.028 kb on - strand, within MMJJ_RS02065 at 393.088 kb on - strand, within MMJJ_RS02065
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 2g/L casaminos remove 390,362 - -1.5 390,362 - -2.5 390,400 - +0.2 390,400 - -1.9 390,400 - -0.4 390,401 + +0.1 390,401 + -1.4 390,401 + +0.8 390,401 + -0.7 390,402 - -0.9 390,404 - -1.1 390,404 - -0.6 390,405 + +0.2 390,405 + -0.9 390,405 + +0.4 390,413 + -2.0 390,417 + +0.4 390,418 - -0.9 390,418 - -0.2 390,430 - -0.3 390,449 - +0.2 390,449 - -3.2 390,450 + -0.3 390,493 + -1.2 390,504 + +0.2 390,513 + +1.0 390,590 - MMJJ_RS02050 0.17 -0.1 390,767 + MMJJ_RS02050 0.43 +0.1 390,825 - MMJJ_RS02050 0.51 -1.2 390,826 + MMJJ_RS02050 0.51 -0.7 390,847 + MMJJ_RS02050 0.54 +0.1 390,849 - MMJJ_RS02050 0.55 -1.3 390,850 + MMJJ_RS02050 0.55 -0.7 390,850 + MMJJ_RS02050 0.55 -2.2 390,939 - MMJJ_RS02050 0.68 +1.0 390,939 - MMJJ_RS02050 0.68 +0.2 390,984 - MMJJ_RS02050 0.74 +0.2 390,990 - MMJJ_RS02050 0.75 -1.0 390,990 - MMJJ_RS02050 0.75 -0.3 391,011 - MMJJ_RS02050 0.78 -0.6 391,011 - MMJJ_RS02050 0.78 -0.3 391,017 - MMJJ_RS02050 0.79 +0.2 391,042 - MMJJ_RS02050 0.83 +0.5 391,042 - MMJJ_RS02050 0.83 -0.5 391,042 - MMJJ_RS02050 0.83 +0.3 391,043 + MMJJ_RS02050 0.83 +0.7 391,043 + MMJJ_RS02050 0.83 +0.2 391,171 - +0.5 391,171 - +0.4 391,172 + +0.4 391,192 - +0.7 391,193 + -0.3 391,193 + -0.3 391,251 + -0.5 391,287 - -4.7 391,346 + -3.0 391,527 + MMJJ_RS02055 0.26 -0.6 391,527 + MMJJ_RS02055 0.26 -1.5 391,527 + MMJJ_RS02055 0.26 -2.7 391,527 + MMJJ_RS02055 0.26 -3.6 391,546 + -2.3 391,564 - MMJJ_RS02055 0.30 -3.0 391,578 - MMJJ_RS02055 0.32 -2.3 391,589 - MMJJ_RS02055 0.33 -2.0 391,590 + MMJJ_RS02055 0.33 -2.6 391,638 - MMJJ_RS02055 0.39 -3.2 391,642 - MMJJ_RS02055 0.39 -2.4 391,643 + MMJJ_RS02055 0.39 -1.9 391,728 - MMJJ_RS02055 0.49 -3.0 391,735 + MMJJ_RS02055 0.50 -2.6 391,735 + MMJJ_RS02055 0.50 -2.2 391,844 + MMJJ_RS02055 0.62 -2.0 391,863 - MMJJ_RS02055 0.64 -4.8 391,863 - MMJJ_RS02055 0.64 -2.6 391,878 - MMJJ_RS02055 0.66 -3.6 391,879 + MMJJ_RS02055 0.66 -2.5 391,879 + MMJJ_RS02055 0.66 -1.9 391,926 - MMJJ_RS02055 0.71 -2.3 391,927 + MMJJ_RS02055 0.71 -2.7 391,927 + MMJJ_RS02055 0.71 -4.2 391,927 + MMJJ_RS02055 0.71 -3.7 391,930 - MMJJ_RS02055 0.71 -2.3 391,961 - MMJJ_RS02055 0.75 -2.7 391,978 + MMJJ_RS02055 0.77 -2.6 392,037 - MMJJ_RS02055 0.83 -2.4 392,045 + MMJJ_RS02055 0.84 -3.9 392,045 + MMJJ_RS02055 0.84 -2.4 392,045 + MMJJ_RS02055 0.84 -2.4 392,057 - MMJJ_RS02055 0.86 -3.3 392,061 - MMJJ_RS02055 0.86 -3.6 392,061 - MMJJ_RS02055 0.86 -3.6 392,086 - MMJJ_RS02055 0.89 -4.0 392,128 - -3.4 392,129 + -3.4 392,129 + -1.7 392,192 - +0.6 392,209 - -0.7 392,217 - -0.1 392,254 - MMJJ_RS02060 0.12 -0.1 392,255 + MMJJ_RS02060 0.12 -0.6 392,270 + MMJJ_RS02060 0.15 +0.7 392,270 + MMJJ_RS02060 0.15 +0.0 392,303 - MMJJ_RS02060 0.22 -0.0 392,315 + MMJJ_RS02060 0.24 +0.4 392,315 + MMJJ_RS02060 0.24 +1.1 392,316 - MMJJ_RS02060 0.24 -1.0 392,317 + MMJJ_RS02060 0.25 +1.0 392,317 + MMJJ_RS02060 0.25 -0.1 392,338 + MMJJ_RS02060 0.29 +0.6 392,347 - MMJJ_RS02060 0.31 +0.1 392,358 + MMJJ_RS02060 0.33 -1.2 392,413 - MMJJ_RS02060 0.44 +0.1 392,416 - MMJJ_RS02060 0.44 +1.0 392,432 - MMJJ_RS02060 0.48 +1.0 392,433 + MMJJ_RS02060 0.48 -2.2 392,436 - MMJJ_RS02060 0.48 -0.2 392,436 - MMJJ_RS02060 0.48 +0.6 392,442 - MMJJ_RS02060 0.50 +0.1 392,443 + MMJJ_RS02060 0.50 -0.4 392,443 + MMJJ_RS02060 0.50 +0.7 392,464 - MMJJ_RS02060 0.54 +0.4 392,464 - MMJJ_RS02060 0.54 -0.9 392,466 - MMJJ_RS02060 0.54 -0.0 392,466 - MMJJ_RS02060 0.54 -1.4 392,466 - MMJJ_RS02060 0.54 -0.4 392,467 + MMJJ_RS02060 0.54 -0.3 392,467 + MMJJ_RS02060 0.54 -0.6 392,487 - MMJJ_RS02060 0.58 -0.3 392,487 - MMJJ_RS02060 0.58 +0.9 392,516 - MMJJ_RS02060 0.64 -1.2 392,516 - MMJJ_RS02060 0.64 -0.1 392,708 - +0.1 392,708 - +0.6 392,709 + -0.3 392,712 + -1.0 392,717 + -0.1 392,717 + -0.1 392,717 + -0.2 392,737 - -0.2 392,738 + -0.4 392,738 + -0.6 392,738 + +0.1 392,739 - -0.2 392,739 - +0.4 392,742 - -1.2 392,743 + -2.6 392,743 + -0.3 392,767 - +0.2 392,767 - +0.6 392,768 + +0.1 392,797 - +0.6 392,797 - +0.2 392,797 - +1.0 392,812 - -0.1 392,817 - +0.2 392,817 - -1.0 392,817 - +0.2 392,817 - -0.4 392,820 - +0.9 392,880 - -1.1 392,881 + +0.3 392,892 - -0.6 392,896 + +1.3 392,918 - +0.7 392,925 - -0.7 393,028 - MMJJ_RS02065 0.16 +0.4 393,088 - MMJJ_RS02065 0.20 -0.1
Or see this region's nucleotide sequence