Experiment: H2 N-free with 2g/L casaminos
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS02020 and MMJJ_RS02025 are separated by 99 nucleotides MMJJ_RS02025 and MMJJ_RS02030 are separated by 80 nucleotides MMJJ_RS02030 and MMJJ_RS02035 are separated by 9 nucleotides MMJJ_RS02035 and MMJJ_RS02040 are separated by 17 nucleotides MMJJ_RS02040 and MMJJ_RS02045 are separated by 542 nucleotides
MMJJ_RS02020: MMJJ_RS02020 - DUF115 domain-containing protein, at 386,661 to 387,377
_RS02020
MMJJ_RS02025: MMJJ_RS02025 - chorismate mutase, at 387,477 to 387,767
_RS02025
MMJJ_RS02030: MMJJ_RS02030 - 30S ribosomal protein S17e, at 387,848 to 388,039
_RS02030
MMJJ_RS02035: MMJJ_RS02035 - 4-hydroxy-tetrahydrodipicolinate synthase, at 388,049 to 388,918
_RS02035
MMJJ_RS02040: MMJJ_RS02040 - Asp-tRNA(Asn) amidotransferase subunit GatC, at 388,936 to 389,175
_RS02040
MMJJ_RS02045: MMJJ_RS02045 - molybdenum cofactor guanylyltransferase, at 389,718 to 390,362
_RS02045
Position (kb) 388
389 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 387.235 kb on + strand, within MMJJ_RS02020 at 387.388 kb on - strand at 387.459 kb on + strand at 387.765 kb on - strand at 387.766 kb on + strand at 387.777 kb on + strand at 387.781 kb on + strand at 387.781 kb on + strand at 387.781 kb on + strand at 387.781 kb on + strand at 387.781 kb on + strand at 387.782 kb on - strand at 387.782 kb on - strand at 387.782 kb on - strand at 387.785 kb on + strand at 387.785 kb on + strand at 387.796 kb on + strand at 387.808 kb on - strand at 387.825 kb on + strand at 387.825 kb on + strand at 387.828 kb on - strand at 387.833 kb on - strand at 388.037 kb on - strand at 388.038 kb on + strand at 388.045 kb on + strand at 388.049 kb on + strand at 388.060 kb on + strand at 388.159 kb on - strand, within MMJJ_RS02035 at 388.203 kb on - strand, within MMJJ_RS02035 at 388.361 kb on - strand at 388.361 kb on - strand, within MMJJ_RS02035 at 388.361 kb on - strand, within MMJJ_RS02035 at 388.362 kb on + strand, within MMJJ_RS02035 at 388.363 kb on - strand, within MMJJ_RS02035 at 388.431 kb on - strand, within MMJJ_RS02035 at 388.470 kb on - strand, within MMJJ_RS02035 at 388.470 kb on - strand, within MMJJ_RS02035 at 388.470 kb on - strand, within MMJJ_RS02035 at 388.471 kb on + strand, within MMJJ_RS02035 at 389.080 kb on + strand, within MMJJ_RS02040 at 389.111 kb on - strand, within MMJJ_RS02040 at 389.125 kb on + strand, within MMJJ_RS02040 at 389.209 kb on + strand at 389.262 kb on + strand at 389.309 kb on - strand at 389.309 kb on - strand at 389.309 kb on - strand at 389.313 kb on - strand at 389.313 kb on - strand at 389.314 kb on + strand at 389.321 kb on - strand at 389.325 kb on - strand at 389.351 kb on - strand at 389.351 kb on - strand at 389.352 kb on + strand at 389.352 kb on + strand at 389.353 kb on - strand at 389.353 kb on - strand at 389.353 kb on - strand at 389.353 kb on - strand at 389.353 kb on - strand at 389.353 kb on - strand at 389.353 kb on - strand at 389.354 kb on + strand at 389.354 kb on + strand at 389.354 kb on + strand at 389.354 kb on + strand at 389.354 kb on + strand at 389.354 kb on + strand at 389.354 kb on + strand at 389.354 kb on + strand at 389.354 kb on + strand at 389.354 kb on + strand at 389.354 kb on + strand at 389.354 kb on + strand at 389.357 kb on - strand at 389.357 kb on - strand at 389.357 kb on - strand at 389.358 kb on + strand at 389.358 kb on + strand at 389.358 kb on + strand at 389.369 kb on - strand at 389.375 kb on - strand at 389.375 kb on - strand at 389.381 kb on - strand at 389.398 kb on + strand at 389.450 kb on - strand at 389.450 kb on - strand at 389.451 kb on + strand at 389.451 kb on + strand at 389.452 kb on - strand at 389.453 kb on + strand at 389.453 kb on + strand at 389.454 kb on - strand at 389.455 kb on + strand at 389.477 kb on + strand at 389.482 kb on - strand at 389.483 kb on + strand at 389.483 kb on + strand at 389.483 kb on + strand at 389.490 kb on - strand at 389.490 kb on - strand at 389.524 kb on - strand at 389.569 kb on - strand at 389.588 kb on - strand at 389.588 kb on - strand at 389.589 kb on + strand at 389.589 kb on + strand at 389.592 kb on - strand at 389.609 kb on + strand at 389.618 kb on - strand at 389.626 kb on + strand at 389.626 kb on + strand at 389.630 kb on + strand at 389.630 kb on + strand at 389.630 kb on + strand at 389.630 kb on + strand at 389.631 kb on - strand at 389.676 kb on - strand at 389.677 kb on + strand at 389.679 kb on - strand at 389.680 kb on + strand at 389.686 kb on - strand at 389.773 kb on + strand at 389.810 kb on + strand, within MMJJ_RS02045 at 389.892 kb on - strand, within MMJJ_RS02045
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 2g/L casaminos remove 387,235 + MMJJ_RS02020 0.80 -2.3 387,388 - +0.6 387,459 + -2.5 387,765 - -1.5 387,766 + -1.8 387,777 + -1.2 387,781 + -3.1 387,781 + -1.4 387,781 + -1.9 387,781 + -2.3 387,781 + -2.4 387,782 - -0.2 387,782 - -3.3 387,782 - -1.2 387,785 + -2.6 387,785 + -2.2 387,796 + -1.2 387,808 - -4.6 387,825 + -1.5 387,825 + -1.7 387,828 - -1.7 387,833 - +0.3 388,037 - -2.7 388,038 + -1.8 388,045 + -3.3 388,049 + -1.8 388,060 + -2.1 388,159 - MMJJ_RS02035 0.13 -1.7 388,203 - MMJJ_RS02035 0.18 -2.7 388,361 - -1.2 388,361 - MMJJ_RS02035 0.36 -0.6 388,361 - MMJJ_RS02035 0.36 -1.4 388,362 + MMJJ_RS02035 0.36 -1.8 388,363 - MMJJ_RS02035 0.36 -2.6 388,431 - MMJJ_RS02035 0.44 -3.9 388,470 - MMJJ_RS02035 0.48 -2.8 388,470 - MMJJ_RS02035 0.48 -0.9 388,470 - MMJJ_RS02035 0.48 -1.8 388,471 + MMJJ_RS02035 0.49 -4.7 389,080 + MMJJ_RS02040 0.60 -1.4 389,111 - MMJJ_RS02040 0.73 -0.6 389,125 + MMJJ_RS02040 0.79 -2.0 389,209 + -2.2 389,262 + -2.4 389,309 - -0.5 389,309 - -0.3 389,309 - +0.2 389,313 - -0.2 389,313 - -0.4 389,314 + -0.8 389,321 - +0.1 389,325 - +1.1 389,351 - +0.4 389,351 - +0.6 389,352 + +0.8 389,352 + -0.9 389,353 - +0.7 389,353 - -0.3 389,353 - -1.8 389,353 - -0.2 389,353 - -0.3 389,353 - +0.7 389,353 - +0.5 389,354 + -0.3 389,354 + +0.1 389,354 + +0.2 389,354 + +0.2 389,354 + +0.0 389,354 + -1.2 389,354 + -0.5 389,354 + -0.6 389,354 + +1.2 389,354 + +1.0 389,354 + +0.0 389,354 + -1.7 389,357 - -1.0 389,357 - +0.2 389,357 - -0.1 389,358 + -0.3 389,358 + -1.1 389,358 + -0.7 389,369 - +0.6 389,375 - +0.3 389,375 - +0.2 389,381 - -0.6 389,398 + +0.4 389,450 - +0.1 389,450 - +2.2 389,451 + +0.4 389,451 + -0.9 389,452 - -1.7 389,453 + -0.5 389,453 + +0.2 389,454 - +0.4 389,455 + -0.2 389,477 + -0.2 389,482 - +0.1 389,483 + -0.0 389,483 + +0.2 389,483 + -0.5 389,490 - -0.3 389,490 - +0.3 389,524 - +1.1 389,569 - +1.2 389,588 - -0.1 389,588 - -0.7 389,589 + +0.5 389,589 + +1.2 389,592 - -0.0 389,609 + -0.2 389,618 - -2.1 389,626 + -0.7 389,626 + +1.7 389,630 + -0.8 389,630 + -0.3 389,630 + -0.5 389,630 + +0.4 389,631 - +0.3 389,676 - -0.1 389,677 + -0.4 389,679 - -0.0 389,680 + +0.2 389,686 - +0.5 389,773 + -0.3 389,810 + MMJJ_RS02045 0.14 -3.9 389,892 - MMJJ_RS02045 0.27 -1.7
Or see this region's nucleotide sequence