Experiment: H2 N-free with 2g/L casaminos
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS01850 and MMJJ_RS01855 are separated by 1 nucleotides MMJJ_RS01855 and MMJJ_RS01860 are separated by 53 nucleotides MMJJ_RS01860 and MMJJ_RS01865 are separated by 147 nucleotides MMJJ_RS01865 and MMJJ_RS01870 are separated by 135 nucleotides
MMJJ_RS01850: MMJJ_RS01850 - RNA 2',3'-cyclic phosphodiesterase, at 355,081 to 355,605
_RS01850
MMJJ_RS01855: MMJJ_RS01855 - MBL fold metallo-hydrolase, at 355,607 to 356,149
_RS01855
MMJJ_RS01860: MMJJ_RS01860 - tRNA guanosine(15) transglycosylase TgtA, at 356,203 to 358,152
_RS01860
MMJJ_RS01865: MMJJ_RS01865 - peptidyl-tRNA hydrolase Pth2, at 358,300 to 358,650
_RS01865
MMJJ_RS01870: MMJJ_RS01870 - methanogenesis marker 15 protein, at 358,786 to 360,021
_RS01870
Position (kb) 356
357
358
359 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 355.318 kb on - strand, within MMJJ_RS01850 at 355.318 kb on - strand, within MMJJ_RS01850 at 355.318 kb on - strand, within MMJJ_RS01850 at 355.319 kb on + strand, within MMJJ_RS01850 at 355.366 kb on + strand, within MMJJ_RS01850 at 355.447 kb on + strand, within MMJJ_RS01850 at 355.487 kb on - strand, within MMJJ_RS01850 at 355.507 kb on + strand, within MMJJ_RS01850 at 355.529 kb on - strand, within MMJJ_RS01850 at 355.530 kb on + strand, within MMJJ_RS01850 at 355.546 kb on + strand, within MMJJ_RS01850 at 355.546 kb on + strand, within MMJJ_RS01850 at 355.554 kb on - strand at 355.564 kb on + strand at 355.579 kb on - strand at 355.614 kb on - strand at 355.627 kb on + strand at 355.651 kb on - strand at 355.651 kb on - strand at 355.652 kb on + strand at 355.673 kb on - strand, within MMJJ_RS01855 at 355.723 kb on - strand, within MMJJ_RS01855 at 355.724 kb on + strand, within MMJJ_RS01855 at 355.779 kb on - strand, within MMJJ_RS01855 at 355.779 kb on - strand, within MMJJ_RS01855 at 355.779 kb on - strand, within MMJJ_RS01855 at 355.779 kb on - strand, within MMJJ_RS01855 at 355.779 kb on - strand, within MMJJ_RS01855 at 355.780 kb on + strand, within MMJJ_RS01855 at 355.780 kb on + strand, within MMJJ_RS01855 at 355.801 kb on - strand, within MMJJ_RS01855 at 355.853 kb on + strand, within MMJJ_RS01855 at 355.862 kb on - strand, within MMJJ_RS01855 at 355.879 kb on + strand, within MMJJ_RS01855 at 355.882 kb on - strand, within MMJJ_RS01855 at 355.901 kb on + strand, within MMJJ_RS01855 at 355.947 kb on + strand, within MMJJ_RS01855 at 355.948 kb on - strand, within MMJJ_RS01855 at 355.948 kb on - strand, within MMJJ_RS01855 at 355.948 kb on - strand, within MMJJ_RS01855 at 355.949 kb on + strand, within MMJJ_RS01855 at 355.949 kb on + strand, within MMJJ_RS01855 at 355.951 kb on - strand, within MMJJ_RS01855 at 355.952 kb on + strand, within MMJJ_RS01855 at 355.952 kb on + strand, within MMJJ_RS01855 at 355.952 kb on + strand, within MMJJ_RS01855 at 355.953 kb on - strand, within MMJJ_RS01855 at 355.954 kb on + strand, within MMJJ_RS01855 at 355.979 kb on - strand, within MMJJ_RS01855 at 355.980 kb on + strand, within MMJJ_RS01855 at 355.980 kb on + strand, within MMJJ_RS01855 at 356.030 kb on + strand, within MMJJ_RS01855 at 356.039 kb on - strand, within MMJJ_RS01855 at 356.060 kb on - strand, within MMJJ_RS01855 at 356.061 kb on + strand, within MMJJ_RS01855 at 356.063 kb on + strand, within MMJJ_RS01855 at 356.064 kb on - strand, within MMJJ_RS01855 at 356.064 kb on - strand, within MMJJ_RS01855 at 356.068 kb on - strand, within MMJJ_RS01855 at 356.069 kb on + strand, within MMJJ_RS01855 at 356.069 kb on + strand, within MMJJ_RS01855 at 356.069 kb on + strand, within MMJJ_RS01855 at 356.069 kb on + strand, within MMJJ_RS01855 at 356.094 kb on + strand, within MMJJ_RS01855 at 356.133 kb on - strand at 356.133 kb on - strand at 356.133 kb on - strand at 356.133 kb on - strand at 356.133 kb on - strand at 356.133 kb on - strand at 356.133 kb on - strand at 356.134 kb on + strand at 356.134 kb on + strand at 356.134 kb on + strand at 356.134 kb on + strand at 356.134 kb on + strand at 356.134 kb on + strand at 356.134 kb on + strand at 356.134 kb on + strand at 356.134 kb on + strand at 356.144 kb on - strand at 356.175 kb on + strand at 356.191 kb on + strand at 356.195 kb on - strand at 356.198 kb on - strand at 356.199 kb on + strand at 356.275 kb on - strand at 356.275 kb on - strand at 356.275 kb on - strand at 356.275 kb on - strand at 356.284 kb on + strand at 356.289 kb on + strand at 356.296 kb on - strand at 356.423 kb on + strand, within MMJJ_RS01860 at 356.426 kb on + strand, within MMJJ_RS01860 at 356.432 kb on - strand, within MMJJ_RS01860 at 356.458 kb on - strand, within MMJJ_RS01860 at 356.514 kb on - strand, within MMJJ_RS01860 at 356.515 kb on + strand, within MMJJ_RS01860 at 356.621 kb on - strand, within MMJJ_RS01860 at 356.701 kb on - strand, within MMJJ_RS01860 at 356.702 kb on + strand, within MMJJ_RS01860 at 356.702 kb on + strand, within MMJJ_RS01860 at 356.702 kb on + strand, within MMJJ_RS01860 at 356.702 kb on + strand, within MMJJ_RS01860 at 356.705 kb on - strand, within MMJJ_RS01860 at 356.706 kb on + strand, within MMJJ_RS01860 at 356.706 kb on + strand, within MMJJ_RS01860 at 356.706 kb on + strand at 356.706 kb on + strand, within MMJJ_RS01860 at 356.708 kb on - strand at 356.708 kb on - strand, within MMJJ_RS01860 at 356.709 kb on + strand, within MMJJ_RS01860 at 356.714 kb on - strand, within MMJJ_RS01860 at 356.714 kb on - strand, within MMJJ_RS01860 at 356.714 kb on - strand, within MMJJ_RS01860 at 356.715 kb on + strand, within MMJJ_RS01860 at 356.718 kb on - strand, within MMJJ_RS01860 at 356.718 kb on - strand, within MMJJ_RS01860 at 356.722 kb on - strand, within MMJJ_RS01860 at 356.723 kb on + strand, within MMJJ_RS01860 at 356.723 kb on + strand, within MMJJ_RS01860 at 356.723 kb on + strand, within MMJJ_RS01860 at 356.778 kb on - strand, within MMJJ_RS01860 at 356.885 kb on + strand, within MMJJ_RS01860 at 356.902 kb on - strand at 356.902 kb on - strand, within MMJJ_RS01860 at 356.902 kb on - strand, within MMJJ_RS01860 at 356.902 kb on - strand, within MMJJ_RS01860 at 356.903 kb on + strand, within MMJJ_RS01860 at 356.903 kb on + strand, within MMJJ_RS01860 at 356.903 kb on + strand, within MMJJ_RS01860 at 356.937 kb on - strand, within MMJJ_RS01860 at 356.956 kb on - strand, within MMJJ_RS01860 at 356.972 kb on - strand, within MMJJ_RS01860 at 357.005 kb on - strand, within MMJJ_RS01860 at 357.018 kb on - strand, within MMJJ_RS01860 at 357.024 kb on - strand, within MMJJ_RS01860 at 357.093 kb on - strand, within MMJJ_RS01860 at 357.095 kb on - strand, within MMJJ_RS01860 at 357.096 kb on + strand, within MMJJ_RS01860 at 357.182 kb on + strand, within MMJJ_RS01860 at 357.198 kb on - strand, within MMJJ_RS01860 at 357.217 kb on + strand, within MMJJ_RS01860 at 357.272 kb on - strand, within MMJJ_RS01860 at 357.418 kb on - strand, within MMJJ_RS01860 at 357.451 kb on - strand, within MMJJ_RS01860 at 357.568 kb on - strand, within MMJJ_RS01860 at 357.568 kb on - strand, within MMJJ_RS01860 at 357.585 kb on + strand, within MMJJ_RS01860 at 357.588 kb on - strand, within MMJJ_RS01860 at 357.609 kb on - strand, within MMJJ_RS01860 at 357.679 kb on + strand at 357.703 kb on + strand, within MMJJ_RS01860 at 357.723 kb on - strand, within MMJJ_RS01860 at 357.724 kb on + strand, within MMJJ_RS01860 at 357.736 kb on - strand, within MMJJ_RS01860 at 357.737 kb on + strand, within MMJJ_RS01860 at 357.737 kb on + strand, within MMJJ_RS01860 at 357.737 kb on + strand, within MMJJ_RS01860 at 357.783 kb on - strand, within MMJJ_RS01860 at 357.854 kb on + strand, within MMJJ_RS01860 at 357.854 kb on + strand, within MMJJ_RS01860 at 357.971 kb on - strand at 357.972 kb on + strand at 357.977 kb on + strand at 357.977 kb on + strand at 357.995 kb on + strand at 358.019 kb on + strand at 358.060 kb on - strand at 358.109 kb on + strand at 358.120 kb on + strand at 358.163 kb on - strand at 358.164 kb on + strand at 358.164 kb on + strand at 358.168 kb on - strand at 358.168 kb on - strand at 358.176 kb on + strand at 358.176 kb on + strand at 358.176 kb on + strand at 358.177 kb on - strand at 358.177 kb on - strand at 358.178 kb on + strand at 358.184 kb on - strand at 358.184 kb on - strand at 358.207 kb on - strand at 358.207 kb on - strand at 358.220 kb on - strand at 358.225 kb on - strand at 358.226 kb on + strand at 358.232 kb on - strand at 358.232 kb on - strand at 358.233 kb on + strand at 358.233 kb on + strand at 358.233 kb on + strand at 358.233 kb on + strand at 358.236 kb on - strand at 358.236 kb on - strand at 358.239 kb on - strand at 358.239 kb on - strand at 358.239 kb on - strand at 358.240 kb on + strand at 358.243 kb on - strand at 358.243 kb on - strand at 358.243 kb on - strand at 358.243 kb on - strand at 358.243 kb on - strand at 358.244 kb on + strand at 358.244 kb on + strand at 358.244 kb on + strand at 358.244 kb on + strand at 358.244 kb on + strand at 358.244 kb on + strand at 358.245 kb on - strand at 358.245 kb on - strand at 358.246 kb on + strand at 358.246 kb on + strand at 358.248 kb on - strand at 358.248 kb on - strand at 358.248 kb on - strand at 358.249 kb on + strand at 358.249 kb on + strand at 358.255 kb on - strand at 358.255 kb on - strand at 358.256 kb on + strand at 358.258 kb on + strand at 358.320 kb on - strand at 358.335 kb on + strand, within MMJJ_RS01865 at 358.335 kb on + strand, within MMJJ_RS01865 at 358.335 kb on + strand, within MMJJ_RS01865 at 358.410 kb on + strand, within MMJJ_RS01865 at 358.410 kb on + strand, within MMJJ_RS01865 at 358.470 kb on + strand, within MMJJ_RS01865 at 358.497 kb on + strand, within MMJJ_RS01865 at 358.535 kb on - strand, within MMJJ_RS01865 at 358.536 kb on + strand, within MMJJ_RS01865 at 358.648 kb on - strand at 358.649 kb on + strand at 358.656 kb on + strand at 358.672 kb on - strand at 358.690 kb on + strand at 358.690 kb on + strand at 358.692 kb on - strand at 358.692 kb on - strand at 358.692 kb on - strand at 358.693 kb on + strand at 358.694 kb on - strand at 358.694 kb on - strand at 358.694 kb on - strand at 358.695 kb on + strand at 358.699 kb on + strand at 358.716 kb on - strand at 358.722 kb on + strand at 358.722 kb on + strand at 358.883 kb on - strand at 359.061 kb on + strand at 359.082 kb on + strand, within MMJJ_RS01870
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 2g/L casaminos remove 355,318 - MMJJ_RS01850 0.45 -0.1 355,318 - MMJJ_RS01850 0.45 -0.4 355,318 - MMJJ_RS01850 0.45 +0.1 355,319 + MMJJ_RS01850 0.45 -0.9 355,366 + MMJJ_RS01850 0.54 -0.6 355,447 + MMJJ_RS01850 0.70 -1.5 355,487 - MMJJ_RS01850 0.77 -0.2 355,507 + MMJJ_RS01850 0.81 -2.8 355,529 - MMJJ_RS01850 0.85 +0.1 355,530 + MMJJ_RS01850 0.86 +1.1 355,546 + MMJJ_RS01850 0.89 -0.2 355,546 + MMJJ_RS01850 0.89 -0.2 355,554 - +0.1 355,564 + +0.6 355,579 - -1.5 355,614 - -2.6 355,627 + -2.2 355,651 - +0.2 355,651 - -0.3 355,652 + -0.2 355,673 - MMJJ_RS01855 0.12 -3.6 355,723 - MMJJ_RS01855 0.21 -0.0 355,724 + MMJJ_RS01855 0.22 -0.2 355,779 - MMJJ_RS01855 0.32 -1.7 355,779 - MMJJ_RS01855 0.32 -0.7 355,779 - MMJJ_RS01855 0.32 -1.5 355,779 - MMJJ_RS01855 0.32 +1.1 355,779 - MMJJ_RS01855 0.32 -0.4 355,780 + MMJJ_RS01855 0.32 -0.1 355,780 + MMJJ_RS01855 0.32 -4.3 355,801 - MMJJ_RS01855 0.36 -0.5 355,853 + MMJJ_RS01855 0.45 -0.4 355,862 - MMJJ_RS01855 0.47 +0.5 355,879 + MMJJ_RS01855 0.50 -0.2 355,882 - MMJJ_RS01855 0.51 +0.9 355,901 + MMJJ_RS01855 0.54 -0.3 355,947 + MMJJ_RS01855 0.63 -0.6 355,948 - MMJJ_RS01855 0.63 +0.2 355,948 - MMJJ_RS01855 0.63 +1.0 355,948 - MMJJ_RS01855 0.63 -1.4 355,949 + MMJJ_RS01855 0.63 +0.8 355,949 + MMJJ_RS01855 0.63 -0.4 355,951 - MMJJ_RS01855 0.63 +0.1 355,952 + MMJJ_RS01855 0.64 -0.1 355,952 + MMJJ_RS01855 0.64 +0.2 355,952 + MMJJ_RS01855 0.64 +0.6 355,953 - MMJJ_RS01855 0.64 +0.2 355,954 + MMJJ_RS01855 0.64 +0.3 355,979 - MMJJ_RS01855 0.69 +0.6 355,980 + MMJJ_RS01855 0.69 +0.3 355,980 + MMJJ_RS01855 0.69 +0.9 356,030 + MMJJ_RS01855 0.78 -0.6 356,039 - MMJJ_RS01855 0.80 +0.6 356,060 - MMJJ_RS01855 0.83 -0.4 356,061 + MMJJ_RS01855 0.84 -0.4 356,063 + MMJJ_RS01855 0.84 +0.4 356,064 - MMJJ_RS01855 0.84 -0.3 356,064 - MMJJ_RS01855 0.84 -0.2 356,068 - MMJJ_RS01855 0.85 -0.8 356,069 + MMJJ_RS01855 0.85 +0.2 356,069 + MMJJ_RS01855 0.85 -0.7 356,069 + MMJJ_RS01855 0.85 +0.6 356,069 + MMJJ_RS01855 0.85 +0.8 356,094 + MMJJ_RS01855 0.90 +0.2 356,133 - -0.9 356,133 - +0.3 356,133 - -0.6 356,133 - +0.1 356,133 - +0.5 356,133 - -1.2 356,133 - +1.1 356,134 + +0.5 356,134 + -0.5 356,134 + +0.7 356,134 + +1.6 356,134 + -0.4 356,134 + -1.0 356,134 + -0.9 356,134 + -0.3 356,134 + -0.3 356,144 - -0.0 356,175 + -0.9 356,191 + +0.6 356,195 - -0.1 356,198 - -1.0 356,199 + -0.1 356,275 - +0.5 356,275 - -0.8 356,275 - -3.3 356,275 - -3.7 356,284 + -1.9 356,289 + -0.8 356,296 - -1.9 356,423 + MMJJ_RS01860 0.11 -1.0 356,426 + MMJJ_RS01860 0.11 -2.2 356,432 - MMJJ_RS01860 0.12 -4.5 356,458 - MMJJ_RS01860 0.13 -4.4 356,514 - MMJJ_RS01860 0.16 -1.5 356,515 + MMJJ_RS01860 0.16 -1.3 356,621 - MMJJ_RS01860 0.21 -1.4 356,701 - MMJJ_RS01860 0.26 -2.0 356,702 + MMJJ_RS01860 0.26 -1.9 356,702 + MMJJ_RS01860 0.26 -1.6 356,702 + MMJJ_RS01860 0.26 -1.7 356,702 + MMJJ_RS01860 0.26 -2.1 356,705 - MMJJ_RS01860 0.26 -3.4 356,706 + MMJJ_RS01860 0.26 -1.4 356,706 + MMJJ_RS01860 0.26 -0.8 356,706 + -0.8 356,706 + MMJJ_RS01860 0.26 +0.2 356,708 - -1.2 356,708 - MMJJ_RS01860 0.26 -1.6 356,709 + MMJJ_RS01860 0.26 -0.5 356,714 - MMJJ_RS01860 0.26 -3.0 356,714 - MMJJ_RS01860 0.26 -4.2 356,714 - MMJJ_RS01860 0.26 -1.8 356,715 + MMJJ_RS01860 0.26 -1.5 356,718 - MMJJ_RS01860 0.26 -3.5 356,718 - MMJJ_RS01860 0.26 -1.3 356,722 - MMJJ_RS01860 0.27 -2.1 356,723 + MMJJ_RS01860 0.27 -0.3 356,723 + MMJJ_RS01860 0.27 -1.6 356,723 + MMJJ_RS01860 0.27 -3.0 356,778 - MMJJ_RS01860 0.29 -1.0 356,885 + MMJJ_RS01860 0.35 -3.6 356,902 - -0.2 356,902 - MMJJ_RS01860 0.36 -1.3 356,902 - MMJJ_RS01860 0.36 -2.3 356,902 - MMJJ_RS01860 0.36 -2.0 356,903 + MMJJ_RS01860 0.36 -2.4 356,903 + MMJJ_RS01860 0.36 -2.6 356,903 + MMJJ_RS01860 0.36 -1.6 356,937 - MMJJ_RS01860 0.38 -2.9 356,956 - MMJJ_RS01860 0.39 -1.2 356,972 - MMJJ_RS01860 0.39 -0.3 357,005 - MMJJ_RS01860 0.41 -1.5 357,018 - MMJJ_RS01860 0.42 -1.3 357,024 - MMJJ_RS01860 0.42 -3.9 357,093 - MMJJ_RS01860 0.46 -1.3 357,095 - MMJJ_RS01860 0.46 -2.3 357,096 + MMJJ_RS01860 0.46 -2.9 357,182 + MMJJ_RS01860 0.50 -0.3 357,198 - MMJJ_RS01860 0.51 -1.1 357,217 + MMJJ_RS01860 0.52 -2.5 357,272 - MMJJ_RS01860 0.55 -3.3 357,418 - MMJJ_RS01860 0.62 -2.0 357,451 - MMJJ_RS01860 0.64 -0.4 357,568 - MMJJ_RS01860 0.70 -3.8 357,568 - MMJJ_RS01860 0.70 -0.9 357,585 + MMJJ_RS01860 0.71 -2.5 357,588 - MMJJ_RS01860 0.71 -1.5 357,609 - MMJJ_RS01860 0.72 -1.3 357,679 + +1.4 357,703 + MMJJ_RS01860 0.77 -4.3 357,723 - MMJJ_RS01860 0.78 -1.1 357,724 + MMJJ_RS01860 0.78 -0.9 357,736 - MMJJ_RS01860 0.79 -0.6 357,737 + MMJJ_RS01860 0.79 -0.8 357,737 + MMJJ_RS01860 0.79 -2.7 357,737 + MMJJ_RS01860 0.79 -3.5 357,783 - MMJJ_RS01860 0.81 -2.0 357,854 + MMJJ_RS01860 0.85 -3.4 357,854 + MMJJ_RS01860 0.85 -3.6 357,971 - -1.8 357,972 + -1.5 357,977 + -3.0 357,977 + -1.7 357,995 + -0.7 358,019 + -1.0 358,060 - -1.4 358,109 + -2.5 358,120 + -1.5 358,163 - -0.6 358,164 + -0.9 358,164 + -1.5 358,168 - +0.4 358,168 - +0.5 358,176 + -0.5 358,176 + +0.2 358,176 + +0.6 358,177 - -0.3 358,177 - -0.3 358,178 + -0.7 358,184 - +0.2 358,184 - +0.1 358,207 - +0.6 358,207 - -1.2 358,220 - -1.2 358,225 - -1.0 358,226 + -0.5 358,232 - -0.9 358,232 - +0.6 358,233 + -0.4 358,233 + -0.2 358,233 + -1.2 358,233 + -0.5 358,236 - +0.7 358,236 - -0.8 358,239 - -0.7 358,239 - +1.0 358,239 - +1.2 358,240 + -0.5 358,243 - +0.1 358,243 - -0.5 358,243 - +0.8 358,243 - -0.6 358,243 - +0.3 358,244 + +0.4 358,244 + +0.6 358,244 + -2.1 358,244 + -0.4 358,244 + +0.9 358,244 + -0.2 358,245 - -1.3 358,245 - +0.8 358,246 + -1.0 358,246 + -0.1 358,248 - -3.7 358,248 - -1.2 358,248 - -2.3 358,249 + +0.3 358,249 + +0.6 358,255 - -1.4 358,255 - +0.2 358,256 + -0.9 358,258 + -0.4 358,320 - -0.6 358,335 + MMJJ_RS01865 0.10 -0.8 358,335 + MMJJ_RS01865 0.10 -1.8 358,335 + MMJJ_RS01865 0.10 -1.7 358,410 + MMJJ_RS01865 0.31 -1.2 358,410 + MMJJ_RS01865 0.31 -0.8 358,470 + MMJJ_RS01865 0.48 -0.7 358,497 + MMJJ_RS01865 0.56 -1.8 358,535 - MMJJ_RS01865 0.67 -2.9 358,536 + MMJJ_RS01865 0.67 -3.1 358,648 - -0.3 358,649 + +0.3 358,656 + -0.2 358,672 - +0.4 358,690 + +0.6 358,690 + -0.9 358,692 - +0.2 358,692 - -1.8 358,692 - -0.8 358,693 + -0.8 358,694 - +1.5 358,694 - +0.6 358,694 - -1.2 358,695 + +0.8 358,699 + -0.4 358,716 - -2.8 358,722 + -0.2 358,722 + -3.5 358,883 - -2.3 359,061 + -0.2 359,082 + MMJJ_RS01870 0.24 -2.6
Or see this region's nucleotide sequence