Experiment: H2 N-free with 2g/L casaminos
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS01800 and MMJJ_RS01805 are separated by 46 nucleotides MMJJ_RS01805 and MMJJ_RS01810 are separated by 19 nucleotides MMJJ_RS01810 and MMJJ_RS01815 are separated by 22 nucleotides MMJJ_RS01815 and MMJJ_RS01820 are separated by 10 nucleotides MMJJ_RS01820 and MMJJ_RS01825 are separated by 105 nucleotides
MMJJ_RS01800: MMJJ_RS01800 - ADP-ribosylglycohydrolase family protein, at 345,524 to 346,432
_RS01800
MMJJ_RS01805: MMJJ_RS01805 - sugar phosphate isomerase/epimerase, at 346,479 to 347,288
_RS01805
MMJJ_RS01810: MMJJ_RS01810 - methyl coenzyme M reductase system, component A2, at 347,308 to 348,903
_RS01810
MMJJ_RS01815: MMJJ_RS01815 - DUF134 domain-containing protein, at 348,926 to 349,321
_RS01815
MMJJ_RS01820: MMJJ_RS01820 - MJ1244 family protein, at 349,332 to 349,661
_RS01820
MMJJ_RS01825: MMJJ_RS01825 - DNA repair and recombination protein RadB, at 349,767 to 350,417
_RS01825
Position (kb)
347
348
349 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 346.317 kb on + strand, within MMJJ_RS01800 at 346.451 kb on - strand at 346.451 kb on - strand at 346.451 kb on - strand at 346.451 kb on - strand at 346.452 kb on + strand at 346.453 kb on - strand at 346.467 kb on - strand at 346.468 kb on + strand at 346.470 kb on + strand at 346.489 kb on - strand at 346.519 kb on + strand at 346.538 kb on + strand at 346.538 kb on + strand at 346.538 kb on + strand at 346.541 kb on - strand at 346.541 kb on - strand at 346.542 kb on + strand at 346.542 kb on + strand at 346.543 kb on - strand at 346.543 kb on - strand at 346.543 kb on - strand at 346.544 kb on + strand at 346.544 kb on + strand at 346.544 kb on + strand at 346.544 kb on + strand at 346.562 kb on + strand at 346.618 kb on - strand, within MMJJ_RS01805 at 346.619 kb on + strand, within MMJJ_RS01805 at 346.619 kb on + strand, within MMJJ_RS01805 at 346.623 kb on - strand, within MMJJ_RS01805 at 346.624 kb on + strand, within MMJJ_RS01805 at 346.624 kb on + strand, within MMJJ_RS01805 at 346.625 kb on - strand, within MMJJ_RS01805 at 346.625 kb on - strand, within MMJJ_RS01805 at 346.626 kb on + strand, within MMJJ_RS01805 at 346.626 kb on + strand, within MMJJ_RS01805 at 346.627 kb on - strand, within MMJJ_RS01805 at 346.628 kb on + strand, within MMJJ_RS01805 at 346.628 kb on + strand, within MMJJ_RS01805 at 346.628 kb on + strand, within MMJJ_RS01805 at 346.663 kb on - strand, within MMJJ_RS01805 at 346.663 kb on - strand, within MMJJ_RS01805 at 346.663 kb on - strand, within MMJJ_RS01805 at 346.663 kb on - strand, within MMJJ_RS01805 at 346.663 kb on - strand, within MMJJ_RS01805 at 346.664 kb on + strand, within MMJJ_RS01805 at 346.680 kb on - strand, within MMJJ_RS01805 at 346.680 kb on - strand, within MMJJ_RS01805 at 346.680 kb on - strand, within MMJJ_RS01805 at 346.680 kb on - strand, within MMJJ_RS01805 at 346.682 kb on - strand, within MMJJ_RS01805 at 346.715 kb on - strand, within MMJJ_RS01805 at 346.733 kb on + strand, within MMJJ_RS01805 at 346.792 kb on - strand, within MMJJ_RS01805 at 346.792 kb on - strand, within MMJJ_RS01805 at 346.793 kb on + strand, within MMJJ_RS01805 at 346.897 kb on - strand, within MMJJ_RS01805 at 346.937 kb on - strand, within MMJJ_RS01805 at 346.938 kb on + strand, within MMJJ_RS01805 at 346.992 kb on - strand, within MMJJ_RS01805 at 346.993 kb on + strand, within MMJJ_RS01805 at 347.070 kb on + strand, within MMJJ_RS01805 at 347.075 kb on - strand, within MMJJ_RS01805 at 347.075 kb on - strand, within MMJJ_RS01805 at 347.076 kb on + strand, within MMJJ_RS01805 at 347.125 kb on - strand, within MMJJ_RS01805 at 347.126 kb on + strand, within MMJJ_RS01805 at 347.165 kb on - strand, within MMJJ_RS01805 at 347.166 kb on + strand, within MMJJ_RS01805 at 347.194 kb on - strand, within MMJJ_RS01805 at 347.203 kb on - strand, within MMJJ_RS01805 at 347.204 kb on + strand, within MMJJ_RS01805 at 347.207 kb on + strand, within MMJJ_RS01805 at 347.247 kb on + strand at 347.265 kb on - strand at 347.266 kb on + strand at 347.278 kb on - strand at 347.454 kb on - strand at 347.459 kb on - strand at 347.681 kb on + strand, within MMJJ_RS01810 at 348.011 kb on + strand, within MMJJ_RS01810 at 348.036 kb on + strand, within MMJJ_RS01810 at 348.850 kb on - strand at 349.022 kb on - strand, within MMJJ_RS01815 at 349.027 kb on - strand, within MMJJ_RS01815 at 349.046 kb on + strand, within MMJJ_RS01815 at 349.065 kb on + strand, within MMJJ_RS01815 at 349.211 kb on - strand, within MMJJ_RS01815 at 349.252 kb on - strand, within MMJJ_RS01815 at 349.256 kb on - strand, within MMJJ_RS01815 at 349.333 kb on - strand at 349.333 kb on - strand at 349.382 kb on - strand, within MMJJ_RS01820 at 349.382 kb on - strand, within MMJJ_RS01820 at 349.382 kb on - strand, within MMJJ_RS01820 at 349.382 kb on - strand, within MMJJ_RS01820 at 349.383 kb on + strand, within MMJJ_RS01820 at 349.383 kb on + strand, within MMJJ_RS01820 at 349.383 kb on + strand, within MMJJ_RS01820 at 349.383 kb on + strand, within MMJJ_RS01820 at 349.480 kb on + strand, within MMJJ_RS01820 at 349.677 kb on - strand at 349.677 kb on - strand at 349.677 kb on - strand at 349.696 kb on - strand at 349.696 kb on - strand at 349.696 kb on - strand at 349.712 kb on - strand at 349.714 kb on - strand at 349.714 kb on - strand at 349.714 kb on - strand at 349.714 kb on - strand at 349.715 kb on + strand at 349.715 kb on + strand at 349.715 kb on + strand at 349.715 kb on + strand at 349.715 kb on + strand at 349.716 kb on - strand at 349.718 kb on - strand at 349.719 kb on + strand at 349.751 kb on + strand at 349.781 kb on - strand at 349.809 kb on - strand at 349.863 kb on + strand, within MMJJ_RS01825 at 349.863 kb on + strand, within MMJJ_RS01825
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 2g/L casaminos remove 346,317 + MMJJ_RS01800 0.87 +0.2 346,451 - -0.8 346,451 - +0.6 346,451 - +0.9 346,451 - +1.1 346,452 + +1.1 346,453 - -0.4 346,467 - -0.0 346,468 + +0.6 346,470 + +0.5 346,489 - +0.4 346,519 + +1.3 346,538 + -0.5 346,538 + -0.3 346,538 + +0.1 346,541 - +0.8 346,541 - +0.1 346,542 + +0.3 346,542 + -0.6 346,543 - +0.0 346,543 - -0.6 346,543 - -0.0 346,544 + -2.2 346,544 + -0.5 346,544 + +0.1 346,544 + +0.3 346,562 + -0.2 346,618 - MMJJ_RS01805 0.17 -0.4 346,619 + MMJJ_RS01805 0.17 -0.9 346,619 + MMJJ_RS01805 0.17 -0.1 346,623 - MMJJ_RS01805 0.18 +0.1 346,624 + MMJJ_RS01805 0.18 -1.0 346,624 + MMJJ_RS01805 0.18 -0.1 346,625 - MMJJ_RS01805 0.18 +0.3 346,625 - MMJJ_RS01805 0.18 +0.5 346,626 + MMJJ_RS01805 0.18 +0.0 346,626 + MMJJ_RS01805 0.18 -0.2 346,627 - MMJJ_RS01805 0.18 -0.2 346,628 + MMJJ_RS01805 0.18 -0.7 346,628 + MMJJ_RS01805 0.18 +0.7 346,628 + MMJJ_RS01805 0.18 +0.7 346,663 - MMJJ_RS01805 0.23 +0.4 346,663 - MMJJ_RS01805 0.23 +1.3 346,663 - MMJJ_RS01805 0.23 -0.5 346,663 - MMJJ_RS01805 0.23 -0.8 346,663 - MMJJ_RS01805 0.23 -0.5 346,664 + MMJJ_RS01805 0.23 +0.5 346,680 - MMJJ_RS01805 0.25 +0.7 346,680 - MMJJ_RS01805 0.25 -1.4 346,680 - MMJJ_RS01805 0.25 -0.9 346,680 - MMJJ_RS01805 0.25 +0.0 346,682 - MMJJ_RS01805 0.25 +2.5 346,715 - MMJJ_RS01805 0.29 +0.8 346,733 + MMJJ_RS01805 0.31 +0.2 346,792 - MMJJ_RS01805 0.39 -0.7 346,792 - MMJJ_RS01805 0.39 +0.3 346,793 + MMJJ_RS01805 0.39 -1.1 346,897 - MMJJ_RS01805 0.52 +0.4 346,937 - MMJJ_RS01805 0.57 +0.5 346,938 + MMJJ_RS01805 0.57 -0.3 346,992 - MMJJ_RS01805 0.63 +0.2 346,993 + MMJJ_RS01805 0.63 +0.8 347,070 + MMJJ_RS01805 0.73 -0.7 347,075 - MMJJ_RS01805 0.74 -0.2 347,075 - MMJJ_RS01805 0.74 +0.3 347,076 + MMJJ_RS01805 0.74 -2.0 347,125 - MMJJ_RS01805 0.80 -1.0 347,126 + MMJJ_RS01805 0.80 -0.0 347,165 - MMJJ_RS01805 0.85 -0.3 347,166 + MMJJ_RS01805 0.85 +0.3 347,194 - MMJJ_RS01805 0.88 +0.4 347,203 - MMJJ_RS01805 0.89 -1.5 347,204 + MMJJ_RS01805 0.90 -2.0 347,207 + MMJJ_RS01805 0.90 -0.5 347,247 + -0.7 347,265 - +0.0 347,266 + -0.7 347,278 - -1.1 347,454 - -2.3 347,459 - -2.2 347,681 + MMJJ_RS01810 0.23 -3.0 348,011 + MMJJ_RS01810 0.44 -4.0 348,036 + MMJJ_RS01810 0.46 -3.6 348,850 - -1.7 349,022 - MMJJ_RS01815 0.24 -1.4 349,027 - MMJJ_RS01815 0.26 -2.5 349,046 + MMJJ_RS01815 0.30 -2.2 349,065 + MMJJ_RS01815 0.35 -2.3 349,211 - MMJJ_RS01815 0.72 +0.3 349,252 - MMJJ_RS01815 0.82 -1.6 349,256 - MMJJ_RS01815 0.83 -4.4 349,333 - -2.0 349,333 - -2.0 349,382 - MMJJ_RS01820 0.15 -1.4 349,382 - MMJJ_RS01820 0.15 -0.8 349,382 - MMJJ_RS01820 0.15 +0.2 349,382 - MMJJ_RS01820 0.15 -1.0 349,383 + MMJJ_RS01820 0.15 -1.8 349,383 + MMJJ_RS01820 0.15 -1.7 349,383 + MMJJ_RS01820 0.15 -3.7 349,383 + MMJJ_RS01820 0.15 -1.4 349,480 + MMJJ_RS01820 0.45 -2.2 349,677 - -0.2 349,677 - -1.0 349,677 - -1.5 349,696 - -1.5 349,696 - +0.1 349,696 - -3.5 349,712 - -1.2 349,714 - -2.9 349,714 - +0.2 349,714 - -1.2 349,714 - +0.0 349,715 + -1.9 349,715 + -3.8 349,715 + -4.6 349,715 + -1.1 349,715 + -1.2 349,716 - -0.3 349,718 - +0.3 349,719 + +1.1 349,751 + -2.9 349,781 - -0.1 349,809 - +0.1 349,863 + MMJJ_RS01825 0.15 -0.1 349,863 + MMJJ_RS01825 0.15 -1.5
Or see this region's nucleotide sequence