Experiment: H2 N-free with 2g/L casaminos
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS01405 and MMJJ_RS01410 are separated by 95 nucleotides MMJJ_RS01410 and MMJJ_RS01415 are separated by 134 nucleotides
MMJJ_RS01405: MMJJ_RS01405 - proline--tRNA ligase, at 272,996 to 274,378
_RS01405
MMJJ_RS01410: MMJJ_RS01410 - archaeal proteasome endopeptidase complex subunit beta, at 274,474 to 275,133
_RS01410
MMJJ_RS01415: MMJJ_RS01415 - beta-CASP ribonuclease aCPSF1, at 275,268 to 277,175
_RS01415
Position (kb) 274
275
276 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 274.376 kb on - strand at 274.376 kb on - strand at 274.376 kb on - strand at 274.376 kb on - strand at 274.377 kb on + strand at 274.377 kb on + strand at 274.377 kb on + strand at 274.377 kb on + strand at 274.377 kb on + strand at 274.414 kb on + strand at 274.428 kb on + strand at 274.428 kb on + strand at 274.428 kb on + strand at 274.430 kb on + strand at 274.430 kb on + strand at 274.430 kb on + strand at 274.430 kb on + strand at 274.441 kb on + strand at 274.445 kb on - strand at 274.446 kb on + strand at 274.446 kb on + strand at 274.446 kb on + strand at 274.446 kb on + strand at 274.457 kb on - strand at 274.458 kb on + strand at 274.460 kb on - strand at 274.460 kb on - strand at 274.460 kb on - strand at 274.461 kb on + strand at 274.471 kb on + strand at 274.493 kb on + strand at 274.504 kb on - strand at 274.504 kb on - strand at 274.504 kb on - strand at 274.505 kb on + strand at 274.505 kb on + strand at 274.505 kb on + strand at 274.532 kb on - strand at 274.532 kb on - strand at 274.557 kb on + strand, within MMJJ_RS01410 at 274.557 kb on + strand, within MMJJ_RS01410 at 274.619 kb on + strand, within MMJJ_RS01410 at 274.619 kb on + strand, within MMJJ_RS01410 at 274.621 kb on + strand, within MMJJ_RS01410 at 274.621 kb on + strand, within MMJJ_RS01410 at 274.633 kb on - strand, within MMJJ_RS01410 at 274.638 kb on + strand, within MMJJ_RS01410 at 274.647 kb on - strand, within MMJJ_RS01410 at 274.649 kb on - strand, within MMJJ_RS01410 at 274.650 kb on + strand, within MMJJ_RS01410 at 274.650 kb on + strand, within MMJJ_RS01410 at 274.735 kb on + strand, within MMJJ_RS01410 at 274.760 kb on - strand, within MMJJ_RS01410 at 274.767 kb on - strand, within MMJJ_RS01410 at 274.811 kb on - strand, within MMJJ_RS01410 at 274.811 kb on - strand, within MMJJ_RS01410 at 274.811 kb on - strand, within MMJJ_RS01410 at 275.026 kb on - strand, within MMJJ_RS01410 at 275.232 kb on + strand at 275.249 kb on + strand at 275.368 kb on - strand at 275.983 kb on - strand, within MMJJ_RS01415
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 2g/L casaminos remove 274,376 - -0.3 274,376 - +0.4 274,376 - -0.3 274,376 - +0.6 274,377 + +0.6 274,377 + +0.0 274,377 + -0.1 274,377 + -0.3 274,377 + +0.1 274,414 + +0.1 274,428 + -1.7 274,428 + +0.0 274,428 + -0.8 274,430 + -1.8 274,430 + -0.6 274,430 + -2.8 274,430 + +0.2 274,441 + -1.2 274,445 - -1.4 274,446 + -2.8 274,446 + -1.9 274,446 + -3.1 274,446 + -2.2 274,457 - -3.0 274,458 + -3.7 274,460 - -3.9 274,460 - -1.2 274,460 - -3.6 274,461 + -1.2 274,471 + -3.1 274,493 + -2.5 274,504 - -2.0 274,504 - -1.7 274,504 - -3.0 274,505 + -2.7 274,505 + -2.5 274,505 + -1.7 274,532 - -3.5 274,532 - -2.1 274,557 + MMJJ_RS01410 0.13 -4.5 274,557 + MMJJ_RS01410 0.13 -1.4 274,619 + MMJJ_RS01410 0.22 -2.9 274,619 + MMJJ_RS01410 0.22 -2.1 274,621 + MMJJ_RS01410 0.22 -1.0 274,621 + MMJJ_RS01410 0.22 -1.8 274,633 - MMJJ_RS01410 0.24 -2.2 274,638 + MMJJ_RS01410 0.25 -1.0 274,647 - MMJJ_RS01410 0.26 -1.2 274,649 - MMJJ_RS01410 0.27 -2.0 274,650 + MMJJ_RS01410 0.27 +0.8 274,650 + MMJJ_RS01410 0.27 -2.0 274,735 + MMJJ_RS01410 0.40 -2.3 274,760 - MMJJ_RS01410 0.43 -2.0 274,767 - MMJJ_RS01410 0.44 -2.0 274,811 - MMJJ_RS01410 0.51 -0.9 274,811 - MMJJ_RS01410 0.51 -1.5 274,811 - MMJJ_RS01410 0.51 -1.2 275,026 - MMJJ_RS01410 0.84 -1.8 275,232 + -1.8 275,249 + -3.3 275,368 - -1.3 275,983 - MMJJ_RS01415 0.37 -2.2
Or see this region's nucleotide sequence