Experiment: H2 N-free with 2g/L casaminos
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS01345 and MMJJ_RS01350 are separated by 204 nucleotides MMJJ_RS01350 and MMJJ_RS01355 are separated by 12 nucleotides
MMJJ_RS01345: MMJJ_RS01345 - (5-formylfuran-3-yl)methyl phosphate synthase, at 259,370 to 260,080
_RS01345
MMJJ_RS01350: MMJJ_RS01350 - sodium:proton antiporter, at 260,285 to 261,538
_RS01350
MMJJ_RS01355: MMJJ_RS01355 - UDP-N-acetyl-D-mannosamine dehydrogenase, at 261,551 to 262,834
_RS01355
Position (kb) 260
261
262 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3
4 at 259.301 kb on + strand at 259.311 kb on + strand at 259.342 kb on - strand at 259.342 kb on - strand at 259.350 kb on - strand at 259.355 kb on - strand at 259.355 kb on - strand at 259.356 kb on + strand at 259.362 kb on - strand at 259.444 kb on - strand, within MMJJ_RS01345 at 259.445 kb on + strand, within MMJJ_RS01345 at 259.466 kb on - strand, within MMJJ_RS01345 at 259.466 kb on - strand at 259.679 kb on - strand, within MMJJ_RS01345 at 259.680 kb on + strand, within MMJJ_RS01345 at 259.831 kb on - strand, within MMJJ_RS01345 at 259.847 kb on - strand, within MMJJ_RS01345 at 259.848 kb on + strand, within MMJJ_RS01345 at 259.848 kb on + strand, within MMJJ_RS01345 at 259.848 kb on + strand, within MMJJ_RS01345 at 259.889 kb on + strand, within MMJJ_RS01345 at 259.889 kb on + strand, within MMJJ_RS01345 at 259.891 kb on + strand, within MMJJ_RS01345 at 260.014 kb on - strand at 260.119 kb on - strand at 260.120 kb on + strand at 260.120 kb on + strand at 260.120 kb on + strand at 260.123 kb on - strand at 260.124 kb on + strand at 260.124 kb on + strand at 260.127 kb on - strand at 260.127 kb on - strand at 260.127 kb on - strand at 260.127 kb on - strand at 260.133 kb on - strand at 260.134 kb on + strand at 260.137 kb on - strand at 260.137 kb on - strand at 260.137 kb on - strand at 260.137 kb on - strand at 260.138 kb on + strand at 260.142 kb on - strand at 260.151 kb on - strand at 260.152 kb on + strand at 260.153 kb on - strand at 260.153 kb on - strand at 260.154 kb on + strand at 260.154 kb on + strand at 260.171 kb on + strand at 260.171 kb on + strand at 260.195 kb on - strand at 260.195 kb on - strand at 260.195 kb on - strand at 260.196 kb on + strand at 260.196 kb on + strand at 260.196 kb on + strand at 260.198 kb on + strand at 260.202 kb on - strand at 260.202 kb on - strand at 260.202 kb on - strand at 260.202 kb on - strand at 260.203 kb on + strand at 260.203 kb on + strand at 260.206 kb on + strand at 260.206 kb on + strand at 260.214 kb on + strand at 260.214 kb on + strand at 260.221 kb on + strand at 260.234 kb on + strand at 260.249 kb on - strand at 260.250 kb on + strand at 260.260 kb on - strand at 260.261 kb on + strand at 260.261 kb on + strand at 260.261 kb on + strand at 260.261 kb on + strand at 260.261 kb on + strand at 260.261 kb on + strand at 260.265 kb on - strand at 260.309 kb on - strand at 260.310 kb on + strand at 260.310 kb on + strand at 260.365 kb on + strand at 260.365 kb on + strand at 260.578 kb on - strand, within MMJJ_RS01350 at 260.601 kb on - strand, within MMJJ_RS01350 at 260.616 kb on - strand, within MMJJ_RS01350 at 260.617 kb on + strand, within MMJJ_RS01350 at 260.618 kb on - strand, within MMJJ_RS01350 at 260.619 kb on + strand, within MMJJ_RS01350 at 260.619 kb on + strand, within MMJJ_RS01350 at 260.619 kb on + strand at 260.692 kb on - strand, within MMJJ_RS01350 at 260.692 kb on - strand, within MMJJ_RS01350 at 260.693 kb on + strand, within MMJJ_RS01350 at 260.776 kb on - strand, within MMJJ_RS01350 at 260.849 kb on + strand, within MMJJ_RS01350 at 260.933 kb on - strand, within MMJJ_RS01350 at 260.947 kb on + strand, within MMJJ_RS01350 at 260.971 kb on + strand at 260.971 kb on + strand at 261.026 kb on - strand, within MMJJ_RS01350 at 261.238 kb on + strand, within MMJJ_RS01350 at 261.255 kb on - strand, within MMJJ_RS01350 at 261.256 kb on + strand, within MMJJ_RS01350 at 261.369 kb on + strand, within MMJJ_RS01350 at 261.380 kb on + strand, within MMJJ_RS01350 at 261.422 kb on + strand at 261.607 kb on - strand at 261.733 kb on + strand, within MMJJ_RS01355 at 261.733 kb on + strand, within MMJJ_RS01355 at 261.805 kb on - strand, within MMJJ_RS01355 at 261.921 kb on - strand, within MMJJ_RS01355 at 262.135 kb on + strand, within MMJJ_RS01355 at 262.137 kb on + strand at 262.139 kb on + strand, within MMJJ_RS01355 at 262.336 kb on + strand, within MMJJ_RS01355 at 262.357 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 2g/L casaminos remove 259,301 + -0.9 259,311 + +0.2 259,342 - -0.4 259,342 - -0.4 259,350 - -1.0 259,355 - +0.4 259,355 - -0.7 259,356 + +0.8 259,362 - -0.8 259,444 - MMJJ_RS01345 0.10 -1.2 259,445 + MMJJ_RS01345 0.11 -1.3 259,466 - MMJJ_RS01345 0.14 -2.0 259,466 - +0.4 259,679 - MMJJ_RS01345 0.43 -0.9 259,680 + MMJJ_RS01345 0.44 -3.6 259,831 - MMJJ_RS01345 0.65 -1.2 259,847 - MMJJ_RS01345 0.67 -0.8 259,848 + MMJJ_RS01345 0.67 -2.9 259,848 + MMJJ_RS01345 0.67 -1.5 259,848 + MMJJ_RS01345 0.67 -3.9 259,889 + MMJJ_RS01345 0.73 -3.5 259,889 + MMJJ_RS01345 0.73 -2.3 259,891 + MMJJ_RS01345 0.73 -2.2 260,014 - -0.8 260,119 - -3.1 260,120 + -1.8 260,120 + -2.6 260,120 + -3.0 260,123 - +0.5 260,124 + -0.7 260,124 + -3.4 260,127 - +0.3 260,127 - -0.9 260,127 - +0.5 260,127 - +0.7 260,133 - -0.7 260,134 + -2.5 260,137 - +1.5 260,137 - -0.8 260,137 - +0.1 260,137 - -0.4 260,138 + -0.3 260,142 - -0.3 260,151 - -0.2 260,152 + +1.5 260,153 - +0.5 260,153 - -0.1 260,154 + -0.4 260,154 + +0.7 260,171 + +1.1 260,171 + -0.1 260,195 - +1.8 260,195 - +0.4 260,195 - -2.3 260,196 + +0.2 260,196 + +0.9 260,196 + +0.5 260,198 + -0.0 260,202 - +1.4 260,202 - -0.5 260,202 - -0.8 260,202 - +0.2 260,203 + -1.9 260,203 + -0.2 260,206 + -0.9 260,206 + -2.6 260,214 + -1.5 260,214 + -2.0 260,221 + -0.3 260,234 + +0.7 260,249 - -1.5 260,250 + +0.7 260,260 - -0.8 260,261 + -0.9 260,261 + -1.4 260,261 + +0.0 260,261 + -0.8 260,261 + +0.1 260,261 + +0.4 260,265 - -2.6 260,309 - -0.9 260,310 + -2.0 260,310 + -2.3 260,365 + -1.9 260,365 + -1.5 260,578 - MMJJ_RS01350 0.23 -0.8 260,601 - MMJJ_RS01350 0.25 -3.3 260,616 - MMJJ_RS01350 0.26 -1.0 260,617 + MMJJ_RS01350 0.26 -2.3 260,618 - MMJJ_RS01350 0.27 -1.3 260,619 + MMJJ_RS01350 0.27 -3.3 260,619 + MMJJ_RS01350 0.27 -3.3 260,619 + -1.2 260,692 - MMJJ_RS01350 0.32 -5.0 260,692 - MMJJ_RS01350 0.32 -2.0 260,693 + MMJJ_RS01350 0.33 -1.3 260,776 - MMJJ_RS01350 0.39 -1.9 260,849 + MMJJ_RS01350 0.45 -1.6 260,933 - MMJJ_RS01350 0.52 -0.9 260,947 + MMJJ_RS01350 0.53 -2.2 260,971 + +0.2 260,971 + +0.2 261,026 - MMJJ_RS01350 0.59 -2.3 261,238 + MMJJ_RS01350 0.76 -1.1 261,255 - MMJJ_RS01350 0.77 -0.4 261,256 + MMJJ_RS01350 0.77 -3.6 261,369 + MMJJ_RS01350 0.86 -2.5 261,380 + MMJJ_RS01350 0.87 -2.6 261,422 + -1.3 261,607 - -2.0 261,733 + MMJJ_RS01355 0.14 -0.2 261,733 + MMJJ_RS01355 0.14 -1.3 261,805 - MMJJ_RS01355 0.20 -0.8 261,921 - MMJJ_RS01355 0.29 -1.5 262,135 + MMJJ_RS01355 0.45 +4.1 262,137 + -1.0 262,139 + MMJJ_RS01355 0.46 -3.1 262,336 + MMJJ_RS01355 0.61 -1.4 262,357 + -1.8
Or see this region's nucleotide sequence