Experiment: H2 N-free with 2g/L casaminos
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS00470 and MMJJ_RS00475 are separated by 92 nucleotides MMJJ_RS00475 and MMJJ_RS00480 are separated by 100 nucleotides
MMJJ_RS00470: MMJJ_RS00470 - SBBP repeat-containing protein, at 84,221 to 87,292
_RS00470
MMJJ_RS00475: MMJJ_RS00475 - bifunctional ornithine acetyltransferase/N-acetylglutamate synthase, at 87,385 to 88,611
_RS00475
MMJJ_RS00480: MMJJ_RS00480 - flippase, at 88,712 to 89,983
_RS00480
Position (kb) 87
88
89 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 86.397 kb on - strand, within MMJJ_RS00470 at 86.405 kb on + strand, within MMJJ_RS00470 at 86.437 kb on + strand, within MMJJ_RS00470 at 86.442 kb on - strand, within MMJJ_RS00470 at 86.443 kb on + strand, within MMJJ_RS00470 at 86.456 kb on - strand, within MMJJ_RS00470 at 86.475 kb on - strand, within MMJJ_RS00470 at 86.541 kb on - strand, within MMJJ_RS00470 at 86.552 kb on + strand, within MMJJ_RS00470 at 86.572 kb on - strand, within MMJJ_RS00470 at 86.572 kb on - strand, within MMJJ_RS00470 at 86.573 kb on + strand, within MMJJ_RS00470 at 86.578 kb on - strand, within MMJJ_RS00470 at 86.579 kb on + strand, within MMJJ_RS00470 at 86.581 kb on - strand, within MMJJ_RS00470 at 86.581 kb on - strand, within MMJJ_RS00470 at 86.582 kb on + strand, within MMJJ_RS00470 at 86.582 kb on + strand, within MMJJ_RS00470 at 86.582 kb on + strand, within MMJJ_RS00470 at 86.598 kb on - strand, within MMJJ_RS00470 at 86.602 kb on - strand, within MMJJ_RS00470 at 86.603 kb on + strand, within MMJJ_RS00470 at 86.603 kb on + strand, within MMJJ_RS00470 at 86.604 kb on - strand, within MMJJ_RS00470 at 86.605 kb on + strand, within MMJJ_RS00470 at 86.605 kb on + strand, within MMJJ_RS00470 at 86.622 kb on - strand, within MMJJ_RS00470 at 86.671 kb on + strand, within MMJJ_RS00470 at 86.693 kb on - strand at 86.693 kb on - strand, within MMJJ_RS00470 at 86.694 kb on + strand, within MMJJ_RS00470 at 86.696 kb on - strand, within MMJJ_RS00470 at 86.697 kb on + strand, within MMJJ_RS00470 at 86.708 kb on + strand, within MMJJ_RS00470 at 86.708 kb on + strand, within MMJJ_RS00470 at 86.732 kb on - strand, within MMJJ_RS00470 at 86.732 kb on - strand, within MMJJ_RS00470 at 86.732 kb on - strand, within MMJJ_RS00470 at 86.739 kb on - strand, within MMJJ_RS00470 at 86.772 kb on - strand, within MMJJ_RS00470 at 86.790 kb on - strand, within MMJJ_RS00470 at 86.791 kb on + strand, within MMJJ_RS00470 at 86.794 kb on - strand, within MMJJ_RS00470 at 86.795 kb on + strand, within MMJJ_RS00470 at 86.795 kb on + strand, within MMJJ_RS00470 at 86.795 kb on + strand, within MMJJ_RS00470 at 86.795 kb on + strand, within MMJJ_RS00470 at 86.875 kb on - strand, within MMJJ_RS00470 at 86.875 kb on - strand, within MMJJ_RS00470 at 86.876 kb on + strand, within MMJJ_RS00470 at 86.876 kb on + strand, within MMJJ_RS00470 at 86.876 kb on + strand, within MMJJ_RS00470 at 86.887 kb on - strand, within MMJJ_RS00470 at 86.919 kb on - strand, within MMJJ_RS00470 at 86.919 kb on - strand, within MMJJ_RS00470 at 86.920 kb on + strand, within MMJJ_RS00470 at 86.920 kb on + strand, within MMJJ_RS00470 at 86.920 kb on + strand, within MMJJ_RS00470 at 86.920 kb on + strand, within MMJJ_RS00470 at 86.934 kb on - strand, within MMJJ_RS00470 at 87.004 kb on - strand at 87.005 kb on + strand at 87.010 kb on + strand at 87.010 kb on + strand at 87.010 kb on + strand at 87.010 kb on + strand at 87.010 kb on + strand at 87.029 kb on - strand at 87.029 kb on - strand at 87.032 kb on + strand at 87.050 kb on - strand at 87.051 kb on + strand at 87.118 kb on + strand at 87.158 kb on - strand at 87.159 kb on + strand at 87.168 kb on - strand at 87.168 kb on - strand at 87.168 kb on - strand at 87.168 kb on - strand at 87.168 kb on - strand at 87.170 kb on - strand at 87.170 kb on - strand at 87.170 kb on - strand at 87.170 kb on - strand at 87.172 kb on - strand at 87.172 kb on - strand at 87.172 kb on - strand at 87.293 kb on - strand at 87.306 kb on - strand at 87.387 kb on + strand at 87.387 kb on + strand at 87.393 kb on - strand at 87.393 kb on - strand at 87.393 kb on - strand at 87.394 kb on + strand at 87.395 kb on - strand at 87.417 kb on + strand at 87.724 kb on + strand, within MMJJ_RS00475 at 87.917 kb on + strand, within MMJJ_RS00475 at 88.012 kb on - strand, within MMJJ_RS00475 at 88.477 kb on + strand, within MMJJ_RS00475 at 88.558 kb on + strand at 88.687 kb on - strand at 88.688 kb on + strand at 88.692 kb on + strand at 88.692 kb on + strand at 88.692 kb on + strand at 88.718 kb on + strand at 88.720 kb on - strand at 88.741 kb on - strand at 88.750 kb on - strand at 88.750 kb on - strand at 88.751 kb on + strand at 88.766 kb on - strand at 88.766 kb on - strand at 88.822 kb on - strand at 88.822 kb on - strand at 88.822 kb on - strand at 88.822 kb on - strand at 88.822 kb on - strand at 88.822 kb on - strand at 88.823 kb on + strand at 88.823 kb on + strand at 88.823 kb on + strand at 88.823 kb on + strand at 88.823 kb on + strand at 88.823 kb on + strand at 88.823 kb on + strand at 88.823 kb on + strand at 88.823 kb on + strand at 88.840 kb on + strand, within MMJJ_RS00480 at 88.843 kb on - strand, within MMJJ_RS00480 at 88.844 kb on + strand, within MMJJ_RS00480 at 88.859 kb on - strand, within MMJJ_RS00480 at 88.874 kb on - strand, within MMJJ_RS00480 at 88.882 kb on + strand, within MMJJ_RS00480 at 88.882 kb on + strand, within MMJJ_RS00480 at 88.884 kb on + strand, within MMJJ_RS00480 at 88.933 kb on - strand, within MMJJ_RS00480 at 88.935 kb on - strand, within MMJJ_RS00480 at 88.935 kb on - strand, within MMJJ_RS00480 at 88.936 kb on + strand, within MMJJ_RS00480 at 88.936 kb on + strand, within MMJJ_RS00480 at 88.968 kb on + strand, within MMJJ_RS00480 at 88.996 kb on - strand, within MMJJ_RS00480 at 88.997 kb on + strand, within MMJJ_RS00480 at 89.001 kb on + strand, within MMJJ_RS00480 at 89.018 kb on - strand, within MMJJ_RS00480 at 89.018 kb on - strand, within MMJJ_RS00480 at 89.060 kb on - strand, within MMJJ_RS00480 at 89.141 kb on - strand, within MMJJ_RS00480 at 89.142 kb on + strand, within MMJJ_RS00480 at 89.214 kb on - strand, within MMJJ_RS00480 at 89.234 kb on - strand, within MMJJ_RS00480 at 89.235 kb on + strand, within MMJJ_RS00480 at 89.281 kb on - strand, within MMJJ_RS00480 at 89.304 kb on - strand, within MMJJ_RS00480 at 89.304 kb on - strand at 89.304 kb on - strand, within MMJJ_RS00480 at 89.305 kb on + strand, within MMJJ_RS00480 at 89.305 kb on + strand, within MMJJ_RS00480 at 89.305 kb on + strand, within MMJJ_RS00480 at 89.305 kb on + strand, within MMJJ_RS00480 at 89.307 kb on + strand, within MMJJ_RS00480 at 89.307 kb on + strand, within MMJJ_RS00480 at 89.403 kb on - strand, within MMJJ_RS00480 at 89.475 kb on - strand, within MMJJ_RS00480 at 89.475 kb on - strand, within MMJJ_RS00480 at 89.564 kb on - strand, within MMJJ_RS00480 at 89.607 kb on + strand, within MMJJ_RS00480
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 2g/L casaminos remove 86,397 - MMJJ_RS00470 0.71 +0.5 86,405 + MMJJ_RS00470 0.71 +0.6 86,437 + MMJJ_RS00470 0.72 -0.3 86,442 - MMJJ_RS00470 0.72 +0.1 86,443 + MMJJ_RS00470 0.72 +1.3 86,456 - MMJJ_RS00470 0.73 +1.3 86,475 - MMJJ_RS00470 0.73 -1.5 86,541 - MMJJ_RS00470 0.76 +0.6 86,552 + MMJJ_RS00470 0.76 -0.2 86,572 - MMJJ_RS00470 0.77 -0.2 86,572 - MMJJ_RS00470 0.77 +0.3 86,573 + MMJJ_RS00470 0.77 -0.6 86,578 - MMJJ_RS00470 0.77 +1.0 86,579 + MMJJ_RS00470 0.77 -0.2 86,581 - MMJJ_RS00470 0.77 +0.8 86,581 - MMJJ_RS00470 0.77 +0.2 86,582 + MMJJ_RS00470 0.77 +0.3 86,582 + MMJJ_RS00470 0.77 +0.6 86,582 + MMJJ_RS00470 0.77 +0.8 86,598 - MMJJ_RS00470 0.77 -0.5 86,602 - MMJJ_RS00470 0.78 +0.4 86,603 + MMJJ_RS00470 0.78 +0.8 86,603 + MMJJ_RS00470 0.78 +1.0 86,604 - MMJJ_RS00470 0.78 -0.3 86,605 + MMJJ_RS00470 0.78 -0.1 86,605 + MMJJ_RS00470 0.78 -3.1 86,622 - MMJJ_RS00470 0.78 +0.1 86,671 + MMJJ_RS00470 0.80 -1.0 86,693 - -2.0 86,693 - MMJJ_RS00470 0.80 +0.4 86,694 + MMJJ_RS00470 0.81 -0.3 86,696 - MMJJ_RS00470 0.81 -1.7 86,697 + MMJJ_RS00470 0.81 +0.3 86,708 + MMJJ_RS00470 0.81 +0.8 86,708 + MMJJ_RS00470 0.81 +0.2 86,732 - MMJJ_RS00470 0.82 -0.7 86,732 - MMJJ_RS00470 0.82 +0.5 86,732 - MMJJ_RS00470 0.82 -0.6 86,739 - MMJJ_RS00470 0.82 -0.1 86,772 - MMJJ_RS00470 0.83 +0.3 86,790 - MMJJ_RS00470 0.84 +0.2 86,791 + MMJJ_RS00470 0.84 -1.5 86,794 - MMJJ_RS00470 0.84 +0.1 86,795 + MMJJ_RS00470 0.84 +1.7 86,795 + MMJJ_RS00470 0.84 -0.2 86,795 + MMJJ_RS00470 0.84 -0.6 86,795 + MMJJ_RS00470 0.84 +0.0 86,875 - MMJJ_RS00470 0.86 +0.1 86,875 - MMJJ_RS00470 0.86 +0.9 86,876 + MMJJ_RS00470 0.86 +0.8 86,876 + MMJJ_RS00470 0.86 +0.3 86,876 + MMJJ_RS00470 0.86 +0.0 86,887 - MMJJ_RS00470 0.87 +0.4 86,919 - MMJJ_RS00470 0.88 +0.2 86,919 - MMJJ_RS00470 0.88 +1.1 86,920 + MMJJ_RS00470 0.88 -1.0 86,920 + MMJJ_RS00470 0.88 -0.5 86,920 + MMJJ_RS00470 0.88 -0.4 86,920 + MMJJ_RS00470 0.88 +2.2 86,934 - MMJJ_RS00470 0.88 -0.2 87,004 - -0.4 87,005 + +0.3 87,010 + -0.3 87,010 + -0.2 87,010 + +0.2 87,010 + +0.3 87,010 + -0.2 87,029 - -0.5 87,029 - +0.3 87,032 + -0.7 87,050 - -0.5 87,051 + +0.9 87,118 + -2.1 87,158 - -0.4 87,159 + +0.4 87,168 - -1.2 87,168 - -0.5 87,168 - +0.2 87,168 - -0.8 87,168 - -0.2 87,170 - +1.0 87,170 - +0.7 87,170 - -1.2 87,170 - +0.0 87,172 - +1.0 87,172 - +0.8 87,172 - -0.7 87,293 - +0.7 87,306 - +0.5 87,387 + -0.1 87,387 + +0.4 87,393 - +1.0 87,393 - -1.3 87,393 - +0.1 87,394 + -0.2 87,395 - +0.5 87,417 + -3.0 87,724 + MMJJ_RS00475 0.28 -3.5 87,917 + MMJJ_RS00475 0.43 -3.3 88,012 - MMJJ_RS00475 0.51 -1.5 88,477 + MMJJ_RS00475 0.89 -2.6 88,558 + +0.1 88,687 - -0.7 88,688 + -0.8 88,692 + +0.2 88,692 + +1.0 88,692 + -0.0 88,718 + -0.2 88,720 - +0.1 88,741 - +0.4 88,750 - -0.5 88,750 - -0.0 88,751 + -1.3 88,766 - +0.9 88,766 - +1.2 88,822 - +0.2 88,822 - +0.7 88,822 - -1.0 88,822 - +1.0 88,822 - -0.8 88,822 - -1.1 88,823 + -0.5 88,823 + +0.3 88,823 + -0.1 88,823 + -0.2 88,823 + +0.3 88,823 + +0.4 88,823 + -1.1 88,823 + -1.8 88,823 + -0.9 88,840 + MMJJ_RS00480 0.10 +0.1 88,843 - MMJJ_RS00480 0.10 -0.3 88,844 + MMJJ_RS00480 0.10 +0.2 88,859 - MMJJ_RS00480 0.12 -0.1 88,874 - MMJJ_RS00480 0.13 +0.5 88,882 + MMJJ_RS00480 0.13 +0.4 88,882 + MMJJ_RS00480 0.13 -0.7 88,884 + MMJJ_RS00480 0.14 -1.1 88,933 - MMJJ_RS00480 0.17 -1.2 88,935 - MMJJ_RS00480 0.18 -1.2 88,935 - MMJJ_RS00480 0.18 +0.0 88,936 + MMJJ_RS00480 0.18 -1.8 88,936 + MMJJ_RS00480 0.18 -0.4 88,968 + MMJJ_RS00480 0.20 -0.3 88,996 - MMJJ_RS00480 0.22 -0.5 88,997 + MMJJ_RS00480 0.22 -1.3 89,001 + MMJJ_RS00480 0.23 -0.7 89,018 - MMJJ_RS00480 0.24 -1.3 89,018 - MMJJ_RS00480 0.24 -0.5 89,060 - MMJJ_RS00480 0.27 -1.9 89,141 - MMJJ_RS00480 0.34 -1.2 89,142 + MMJJ_RS00480 0.34 +0.7 89,214 - MMJJ_RS00480 0.39 -1.6 89,234 - MMJJ_RS00480 0.41 -0.2 89,235 + MMJJ_RS00480 0.41 -1.5 89,281 - MMJJ_RS00480 0.45 -0.0 89,304 - MMJJ_RS00480 0.47 -0.0 89,304 - -0.7 89,304 - MMJJ_RS00480 0.47 -0.3 89,305 + MMJJ_RS00480 0.47 -1.0 89,305 + MMJJ_RS00480 0.47 +0.5 89,305 + MMJJ_RS00480 0.47 +0.2 89,305 + MMJJ_RS00480 0.47 -0.7 89,307 + MMJJ_RS00480 0.47 +0.2 89,307 + MMJJ_RS00480 0.47 -1.7 89,403 - MMJJ_RS00480 0.54 -0.4 89,475 - MMJJ_RS00480 0.60 +0.5 89,475 - MMJJ_RS00480 0.60 -0.0 89,564 - MMJJ_RS00480 0.67 +0.3 89,607 + MMJJ_RS00480 0.70 -0.8
Or see this region's nucleotide sequence