Experiment: H2 N-free with 2g/L casaminos
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS00120 and MMJJ_RS00125 are separated by 17 nucleotides MMJJ_RS00125 and MMJJ_RS00130 are separated by 256 nucleotides
MMJJ_RS00120: MMJJ_RS00120 - histidinol dehydrogenase, at 22,922 to 24,196
_RS00120
MMJJ_RS00125: MMJJ_RS00125 - KEOPS complex subunit Cgi121, at 24,214 to 24,645
_RS00125
MMJJ_RS00130: MMJJ_RS00130 - uroporphyrinogen-III C-methyltransferase, at 24,902 to 25,621
_RS00130
Position (kb) 24
25 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 24.472 kb on - strand, within MMJJ_RS00125 at 24.502 kb on + strand at 24.524 kb on - strand, within MMJJ_RS00125 at 24.528 kb on - strand, within MMJJ_RS00125 at 24.529 kb on + strand, within MMJJ_RS00125 at 24.540 kb on - strand, within MMJJ_RS00125 at 24.553 kb on + strand, within MMJJ_RS00125 at 24.556 kb on - strand, within MMJJ_RS00125 at 24.690 kb on + strand at 24.690 kb on + strand at 24.714 kb on + strand at 24.725 kb on - strand at 24.726 kb on + strand at 24.743 kb on - strand at 24.744 kb on + strand at 24.745 kb on - strand at 24.745 kb on - strand at 24.749 kb on - strand at 24.753 kb on - strand at 24.754 kb on + strand at 24.779 kb on + strand at 24.796 kb on + strand at 24.817 kb on + strand at 24.829 kb on + strand at 24.833 kb on + strand at 24.855 kb on - strand at 24.855 kb on - strand at 24.856 kb on + strand at 24.856 kb on + strand at 24.861 kb on + strand at 24.861 kb on + strand at 24.861 kb on + strand at 25.147 kb on + strand, within MMJJ_RS00130 at 25.221 kb on - strand, within MMJJ_RS00130 at 25.246 kb on - strand, within MMJJ_RS00130 at 25.615 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 2g/L casaminos remove 24,472 - MMJJ_RS00125 0.60 -0.7 24,502 + +1.2 24,524 - MMJJ_RS00125 0.72 -2.0 24,528 - MMJJ_RS00125 0.73 -2.6 24,529 + MMJJ_RS00125 0.73 -1.7 24,540 - MMJJ_RS00125 0.75 -2.3 24,553 + MMJJ_RS00125 0.78 -0.2 24,556 - MMJJ_RS00125 0.79 -3.2 24,690 + -2.0 24,690 + -1.0 24,714 + -0.7 24,725 - -3.3 24,726 + -2.5 24,743 - -2.3 24,744 + -0.2 24,745 - -3.1 24,745 - -2.2 24,749 - -0.5 24,753 - +1.3 24,754 + +0.4 24,779 + -1.0 24,796 + +0.7 24,817 + +1.2 24,829 + -1.7 24,833 + -3.1 24,855 - -1.2 24,855 - -3.5 24,856 + -3.7 24,856 + -1.4 24,861 + -1.5 24,861 + +1.4 24,861 + -4.2 25,147 + MMJJ_RS00130 0.34 -2.2 25,221 - MMJJ_RS00130 0.44 -1.5 25,246 - MMJJ_RS00130 0.48 -3.5 25,615 + -1.2
Or see this region's nucleotide sequence