Experiment: R2A with SpecU metal mix 1 X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK54_RS04625 and LRK54_RS04630 are separated by 189 nucleotides LRK54_RS04630 and LRK54_RS04635 are separated by 103 nucleotides LRK54_RS04635 and LRK54_RS04640 are separated by 24 nucleotides
LRK54_RS04625: LRK54_RS04625 - serine hydroxymethyltransferase, at 983,665 to 984,918
_RS04625
LRK54_RS04630: LRK54_RS04630 - energy-dependent translational throttle protein EttA, at 985,108 to 986,775
_RS04630
LRK54_RS04635: LRK54_RS04635 - orotidine-5'-phosphate decarboxylase, at 986,879 to 987,697
_RS04635
LRK54_RS04640: LRK54_RS04640 - hypothetical protein, at 987,722 to 987,979
_RS04640
Position (kb) 985
986
987 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 984.281 kb on + strand, within LRK54_RS04625 at 984.282 kb on - strand, within LRK54_RS04625 at 984.282 kb on - strand, within LRK54_RS04625 at 984.389 kb on + strand, within LRK54_RS04625 at 984.390 kb on - strand, within LRK54_RS04625 at 984.390 kb on - strand, within LRK54_RS04625 at 984.390 kb on - strand, within LRK54_RS04625 at 984.390 kb on - strand, within LRK54_RS04625 at 984.390 kb on - strand, within LRK54_RS04625 at 984.456 kb on + strand, within LRK54_RS04625 at 984.456 kb on - strand, within LRK54_RS04625 at 984.456 kb on - strand, within LRK54_RS04625 at 984.485 kb on + strand, within LRK54_RS04625 at 984.485 kb on + strand, within LRK54_RS04625 at 984.485 kb on + strand, within LRK54_RS04625 at 984.485 kb on + strand, within LRK54_RS04625 at 984.486 kb on - strand, within LRK54_RS04625 at 984.486 kb on - strand, within LRK54_RS04625 at 984.486 kb on - strand, within LRK54_RS04625 at 984.486 kb on - strand, within LRK54_RS04625 at 984.486 kb on - strand, within LRK54_RS04625 at 984.602 kb on + strand, within LRK54_RS04625 at 984.602 kb on + strand, within LRK54_RS04625 at 984.602 kb on + strand, within LRK54_RS04625 at 984.602 kb on + strand, within LRK54_RS04625 at 984.602 kb on + strand, within LRK54_RS04625 at 984.602 kb on + strand, within LRK54_RS04625 at 984.602 kb on + strand, within LRK54_RS04625 at 984.603 kb on - strand, within LRK54_RS04625 at 984.603 kb on - strand, within LRK54_RS04625 at 984.603 kb on - strand, within LRK54_RS04625 at 984.603 kb on - strand, within LRK54_RS04625 at 984.603 kb on - strand, within LRK54_RS04625 at 984.603 kb on - strand, within LRK54_RS04625 at 984.603 kb on - strand, within LRK54_RS04625 at 984.603 kb on - strand, within LRK54_RS04625 at 984.650 kb on + strand, within LRK54_RS04625 at 984.650 kb on + strand, within LRK54_RS04625 at 984.650 kb on + strand, within LRK54_RS04625 at 984.650 kb on + strand, within LRK54_RS04625 at 984.651 kb on - strand, within LRK54_RS04625 at 984.651 kb on - strand, within LRK54_RS04625 at 984.651 kb on - strand, within LRK54_RS04625 at 984.710 kb on + strand, within LRK54_RS04625 at 984.710 kb on + strand, within LRK54_RS04625 at 984.710 kb on + strand, within LRK54_RS04625 at 984.710 kb on + strand, within LRK54_RS04625 at 984.710 kb on + strand, within LRK54_RS04625 at 984.710 kb on + strand, within LRK54_RS04625 at 984.711 kb on - strand, within LRK54_RS04625 at 984.711 kb on - strand, within LRK54_RS04625 at 984.711 kb on - strand, within LRK54_RS04625 at 984.711 kb on - strand, within LRK54_RS04625 at 984.725 kb on + strand, within LRK54_RS04625 at 984.725 kb on + strand, within LRK54_RS04625 at 984.725 kb on + strand, within LRK54_RS04625 at 984.726 kb on - strand, within LRK54_RS04625 at 984.726 kb on - strand, within LRK54_RS04625 at 984.726 kb on - strand, within LRK54_RS04625 at 984.726 kb on - strand, within LRK54_RS04625 at 984.728 kb on + strand, within LRK54_RS04625 at 984.728 kb on + strand, within LRK54_RS04625 at 984.729 kb on - strand, within LRK54_RS04625 at 984.755 kb on + strand, within LRK54_RS04625 at 984.755 kb on + strand, within LRK54_RS04625 at 984.755 kb on + strand, within LRK54_RS04625 at 984.755 kb on + strand, within LRK54_RS04625 at 984.755 kb on + strand, within LRK54_RS04625 at 984.755 kb on + strand, within LRK54_RS04625 at 984.755 kb on + strand, within LRK54_RS04625 at 984.755 kb on + strand, within LRK54_RS04625 at 984.756 kb on - strand, within LRK54_RS04625 at 984.756 kb on - strand, within LRK54_RS04625 at 984.756 kb on - strand, within LRK54_RS04625 at 984.756 kb on - strand, within LRK54_RS04625 at 984.756 kb on - strand, within LRK54_RS04625 at 984.756 kb on - strand, within LRK54_RS04625 at 984.756 kb on - strand, within LRK54_RS04625 at 984.756 kb on - strand, within LRK54_RS04625 at 984.756 kb on - strand, within LRK54_RS04625 at 984.806 kb on + strand at 984.806 kb on + strand at 984.806 kb on + strand at 984.806 kb on + strand at 984.806 kb on + strand at 984.806 kb on + strand at 984.806 kb on + strand at 984.806 kb on + strand at 984.806 kb on + strand at 984.806 kb on + strand at 984.806 kb on + strand at 984.806 kb on + strand at 984.806 kb on + strand at 984.806 kb on + strand at 984.806 kb on + strand at 984.806 kb on + strand at 984.806 kb on + strand at 984.806 kb on + strand at 984.806 kb on + strand at 984.806 kb on + strand at 984.806 kb on + strand at 984.806 kb on + strand at 984.806 kb on + strand at 984.807 kb on - strand at 984.807 kb on - strand at 984.807 kb on - strand at 984.807 kb on - strand at 984.807 kb on - strand at 984.807 kb on - strand at 984.807 kb on - strand at 984.807 kb on - strand at 984.807 kb on - strand at 984.807 kb on - strand at 984.807 kb on - strand at 984.807 kb on - strand at 984.807 kb on - strand at 984.807 kb on - strand at 984.807 kb on - strand at 984.807 kb on - strand at 984.807 kb on - strand at 984.807 kb on - strand at 984.807 kb on - strand at 984.887 kb on + strand at 984.960 kb on + strand at 984.960 kb on + strand at 984.960 kb on + strand at 984.961 kb on - strand at 985.033 kb on + strand at 985.033 kb on + strand at 985.033 kb on + strand at 985.033 kb on + strand at 985.033 kb on + strand at 985.033 kb on + strand at 985.034 kb on - strand at 985.034 kb on - strand at 985.034 kb on - strand at 985.034 kb on - strand at 985.034 kb on - strand at 985.035 kb on + strand at 985.035 kb on + strand at 985.035 kb on + strand at 985.035 kb on + strand at 985.035 kb on + strand at 985.035 kb on + strand at 985.035 kb on + strand at 985.035 kb on + strand at 985.035 kb on + strand at 985.035 kb on + strand at 985.035 kb on + strand at 985.035 kb on + strand at 985.035 kb on + strand at 985.120 kb on + strand at 985.120 kb on + strand at 985.120 kb on + strand at 985.120 kb on + strand at 985.126 kb on + strand at 985.126 kb on + strand at 985.126 kb on + strand at 985.126 kb on + strand at 985.126 kb on + strand at 985.344 kb on + strand, within LRK54_RS04630 at 985.768 kb on + strand at 985.840 kb on + strand, within LRK54_RS04630 at 985.840 kb on + strand, within LRK54_RS04630 at 985.840 kb on + strand, within LRK54_RS04630 at 986.278 kb on + strand, within LRK54_RS04630 at 986.278 kb on + strand, within LRK54_RS04630 at 986.389 kb on + strand, within LRK54_RS04630 at 986.389 kb on + strand, within LRK54_RS04630 at 986.389 kb on + strand, within LRK54_RS04630 at 986.389 kb on + strand, within LRK54_RS04630 at 986.689 kb on + strand at 986.689 kb on + strand at 986.689 kb on + strand at 986.689 kb on + strand at 986.689 kb on + strand at 986.689 kb on + strand at 986.689 kb on + strand at 986.689 kb on + strand at 986.698 kb on + strand at 986.758 kb on + strand at 986.758 kb on + strand at 986.758 kb on + strand at 986.758 kb on + strand at 986.758 kb on + strand at 986.758 kb on + strand at 986.758 kb on + strand at 986.758 kb on + strand at 986.758 kb on + strand at 987.775 kb on + strand, within LRK54_RS04640
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with SpecU metal mix 1 X remove 984,281 + LRK54_RS04625 0.49 -0.8 984,282 - LRK54_RS04625 0.49 -2.7 984,282 - LRK54_RS04625 0.49 -1.3 984,389 + LRK54_RS04625 0.58 -0.3 984,390 - LRK54_RS04625 0.58 -0.3 984,390 - LRK54_RS04625 0.58 -1.3 984,390 - LRK54_RS04625 0.58 -1.8 984,390 - LRK54_RS04625 0.58 -0.7 984,390 - LRK54_RS04625 0.58 +0.2 984,456 + LRK54_RS04625 0.63 -2.1 984,456 - LRK54_RS04625 0.63 -1.8 984,456 - LRK54_RS04625 0.63 -0.8 984,485 + LRK54_RS04625 0.65 -0.8 984,485 + LRK54_RS04625 0.65 -1.6 984,485 + LRK54_RS04625 0.65 -2.3 984,485 + LRK54_RS04625 0.65 -1.8 984,486 - LRK54_RS04625 0.65 -0.3 984,486 - LRK54_RS04625 0.65 -1.8 984,486 - LRK54_RS04625 0.65 -0.3 984,486 - LRK54_RS04625 0.65 -0.3 984,486 - LRK54_RS04625 0.65 -1.3 984,602 + LRK54_RS04625 0.75 -2.3 984,602 + LRK54_RS04625 0.75 -0.3 984,602 + LRK54_RS04625 0.75 -0.6 984,602 + LRK54_RS04625 0.75 -2.4 984,602 + LRK54_RS04625 0.75 -2.8 984,602 + LRK54_RS04625 0.75 -3.0 984,602 + LRK54_RS04625 0.75 -2.4 984,603 - LRK54_RS04625 0.75 -1.6 984,603 - LRK54_RS04625 0.75 +0.3 984,603 - LRK54_RS04625 0.75 -0.1 984,603 - LRK54_RS04625 0.75 -0.9 984,603 - LRK54_RS04625 0.75 -1.8 984,603 - LRK54_RS04625 0.75 -1.5 984,603 - LRK54_RS04625 0.75 -0.4 984,603 - LRK54_RS04625 0.75 -0.7 984,650 + LRK54_RS04625 0.79 -1.3 984,650 + LRK54_RS04625 0.79 +0.2 984,650 + LRK54_RS04625 0.79 -0.3 984,650 + LRK54_RS04625 0.79 -0.4 984,651 - LRK54_RS04625 0.79 -2.1 984,651 - LRK54_RS04625 0.79 -2.3 984,651 - LRK54_RS04625 0.79 -0.1 984,710 + LRK54_RS04625 0.83 -0.4 984,710 + LRK54_RS04625 0.83 +0.7 984,710 + LRK54_RS04625 0.83 -1.3 984,710 + LRK54_RS04625 0.83 -0.6 984,710 + LRK54_RS04625 0.83 -0.3 984,710 + LRK54_RS04625 0.83 -1.3 984,711 - LRK54_RS04625 0.83 -0.6 984,711 - LRK54_RS04625 0.83 -0.8 984,711 - LRK54_RS04625 0.83 -0.8 984,711 - LRK54_RS04625 0.83 -0.3 984,725 + LRK54_RS04625 0.85 -0.3 984,725 + LRK54_RS04625 0.85 +0.3 984,725 + LRK54_RS04625 0.85 -0.2 984,726 - LRK54_RS04625 0.85 -1.3 984,726 - LRK54_RS04625 0.85 +1.3 984,726 - LRK54_RS04625 0.85 -1.3 984,726 - LRK54_RS04625 0.85 -1.6 984,728 + LRK54_RS04625 0.85 -0.8 984,728 + LRK54_RS04625 0.85 -1.7 984,729 - LRK54_RS04625 0.85 -0.8 984,755 + LRK54_RS04625 0.87 -1.8 984,755 + LRK54_RS04625 0.87 +0.7 984,755 + LRK54_RS04625 0.87 +0.5 984,755 + LRK54_RS04625 0.87 +0.7 984,755 + LRK54_RS04625 0.87 +0.3 984,755 + LRK54_RS04625 0.87 -0.8 984,755 + LRK54_RS04625 0.87 +0.7 984,755 + LRK54_RS04625 0.87 -0.4 984,756 - LRK54_RS04625 0.87 -2.3 984,756 - LRK54_RS04625 0.87 -1.5 984,756 - LRK54_RS04625 0.87 -1.3 984,756 - LRK54_RS04625 0.87 -0.5 984,756 - LRK54_RS04625 0.87 -1.0 984,756 - LRK54_RS04625 0.87 +0.7 984,756 - LRK54_RS04625 0.87 -2.0 984,756 - LRK54_RS04625 0.87 -0.1 984,756 - LRK54_RS04625 0.87 +0.2 984,806 + -1.3 984,806 + -0.8 984,806 + -2.6 984,806 + -0.6 984,806 + -1.1 984,806 + +0.7 984,806 + +0.3 984,806 + +0.3 984,806 + -1.6 984,806 + -0.6 984,806 + -1.3 984,806 + -0.3 984,806 + -0.8 984,806 + -1.1 984,806 + +0.7 984,806 + -0.3 984,806 + -2.4 984,806 + -2.4 984,806 + -2.0 984,806 + -0.1 984,806 + -2.2 984,806 + -1.8 984,806 + -2.1 984,807 - -2.3 984,807 - +0.2 984,807 - -0.8 984,807 - -1.3 984,807 - -1.7 984,807 - -0.6 984,807 - -0.3 984,807 - -1.8 984,807 - -1.4 984,807 - -2.3 984,807 - -1.2 984,807 - -1.3 984,807 - -0.8 984,807 - -0.8 984,807 - -0.6 984,807 - -1.6 984,807 - +0.3 984,807 - -0.8 984,807 - -1.1 984,887 + -0.1 984,960 + +0.0 984,960 + -0.8 984,960 + -0.7 984,961 - +0.3 985,033 + +2.2 985,033 + +0.3 985,033 + -1.5 985,033 + -0.3 985,033 + -0.6 985,033 + +0.0 985,034 - -1.3 985,034 - -0.9 985,034 - -1.1 985,034 - +0.1 985,034 - +0.4 985,035 + +0.9 985,035 + -0.4 985,035 + +0.1 985,035 + +0.6 985,035 + -0.5 985,035 + +0.1 985,035 + +0.4 985,035 + -1.3 985,035 + +0.2 985,035 + -1.1 985,035 + -0.1 985,035 + -0.8 985,035 + -0.3 985,120 + +0.7 985,120 + -0.5 985,120 + -0.8 985,120 + +0.7 985,126 + -0.3 985,126 + -0.7 985,126 + -0.8 985,126 + -0.8 985,126 + +0.7 985,344 + LRK54_RS04630 0.14 -0.8 985,768 + +0.7 985,840 + LRK54_RS04630 0.44 +1.7 985,840 + LRK54_RS04630 0.44 -0.3 985,840 + LRK54_RS04630 0.44 -0.3 986,278 + LRK54_RS04630 0.70 -1.3 986,278 + LRK54_RS04630 0.70 -0.8 986,389 + LRK54_RS04630 0.77 +0.7 986,389 + LRK54_RS04630 0.77 -0.3 986,389 + LRK54_RS04630 0.77 -1.3 986,389 + LRK54_RS04630 0.77 -0.8 986,689 + +0.2 986,689 + -1.3 986,689 + -2.3 986,689 + -2.3 986,689 + -1.8 986,689 + -1.8 986,689 + -1.8 986,689 + +0.2 986,698 + -0.8 986,758 + -0.2 986,758 + -0.7 986,758 + +0.1 986,758 + -2.3 986,758 + +0.0 986,758 + -0.8 986,758 + +0.7 986,758 + -0.4 986,758 + +0.3 987,775 + LRK54_RS04640 0.21 +0.5
Or see this region's nucleotide sequence