Experiment: R2A with SpecU metal mix 1 X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK54_RS04575 and LRK54_RS04580 overlap by 1 nucleotides LRK54_RS04580 and LRK54_RS04585 are separated by 110 nucleotides LRK54_RS04585 and LRK54_RS04590 are separated by 83 nucleotides
LRK54_RS04575: LRK54_RS04575 - transcription antitermination factor NusB, at 974,816 to 975,268
_RS04575
LRK54_RS04580: LRK54_RS04580 - 6,7-dimethyl-8-ribityllumazine synthase, at 975,268 to 975,729
_RS04580
LRK54_RS04585: LRK54_RS04585 - muramoyltetrapeptide carboxypeptidase, at 975,840 to 976,784
_RS04585
LRK54_RS04590: LRK54_RS04590 - 3,4-dihydroxy-2-butanone-4-phosphate synthase, at 976,868 to 977,959
_RS04590
Position (kb) 975
976
977 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 976.027 kb on + strand, within LRK54_RS04585 at 976.039 kb on + strand, within LRK54_RS04585 at 976.039 kb on + strand, within LRK54_RS04585 at 976.040 kb on - strand, within LRK54_RS04585 at 976.120 kb on + strand, within LRK54_RS04585 at 976.120 kb on + strand, within LRK54_RS04585 at 976.121 kb on - strand, within LRK54_RS04585 at 976.121 kb on - strand, within LRK54_RS04585 at 976.168 kb on + strand, within LRK54_RS04585 at 976.168 kb on + strand, within LRK54_RS04585 at 976.168 kb on + strand, within LRK54_RS04585 at 976.168 kb on + strand, within LRK54_RS04585 at 976.168 kb on + strand, within LRK54_RS04585 at 976.168 kb on + strand, within LRK54_RS04585 at 976.168 kb on + strand, within LRK54_RS04585 at 976.169 kb on - strand, within LRK54_RS04585 at 976.169 kb on - strand, within LRK54_RS04585 at 976.169 kb on - strand, within LRK54_RS04585 at 976.169 kb on - strand, within LRK54_RS04585 at 976.169 kb on - strand, within LRK54_RS04585 at 976.169 kb on - strand, within LRK54_RS04585 at 976.169 kb on - strand, within LRK54_RS04585 at 976.169 kb on - strand, within LRK54_RS04585 at 976.213 kb on + strand, within LRK54_RS04585 at 976.213 kb on + strand, within LRK54_RS04585 at 976.213 kb on + strand, within LRK54_RS04585 at 976.213 kb on + strand, within LRK54_RS04585 at 976.214 kb on - strand, within LRK54_RS04585 at 976.214 kb on - strand, within LRK54_RS04585 at 976.214 kb on - strand, within LRK54_RS04585 at 976.214 kb on - strand, within LRK54_RS04585 at 976.214 kb on - strand, within LRK54_RS04585 at 976.215 kb on - strand, within LRK54_RS04585 at 976.481 kb on + strand, within LRK54_RS04585 at 976.516 kb on + strand, within LRK54_RS04585 at 976.516 kb on + strand, within LRK54_RS04585 at 976.516 kb on + strand, within LRK54_RS04585 at 976.517 kb on - strand, within LRK54_RS04585 at 976.517 kb on - strand, within LRK54_RS04585 at 976.643 kb on - strand, within LRK54_RS04585 at 976.813 kb on + strand at 976.813 kb on + strand at 976.813 kb on + strand at 976.813 kb on + strand at 976.814 kb on - strand at 976.814 kb on - strand at 976.814 kb on - strand at 976.875 kb on + strand at 976.875 kb on + strand at 976.875 kb on + strand at 976.875 kb on + strand at 976.875 kb on + strand at 976.875 kb on + strand at 976.875 kb on + strand at 976.875 kb on + strand at 976.876 kb on - strand at 976.876 kb on - strand at 976.876 kb on - strand at 976.876 kb on - strand at 976.876 kb on - strand at 976.876 kb on - strand at 976.876 kb on - strand at 976.876 kb on - strand at 976.876 kb on - strand at 976.876 kb on - strand at 976.876 kb on - strand at 976.876 kb on - strand at 976.876 kb on - strand at 976.876 kb on - strand at 976.876 kb on - strand at 976.876 kb on - strand at 976.876 kb on - strand at 976.876 kb on - strand at 977.055 kb on - strand, within LRK54_RS04590 at 977.253 kb on + strand, within LRK54_RS04590 at 977.253 kb on + strand, within LRK54_RS04590 at 977.253 kb on + strand, within LRK54_RS04590 at 977.253 kb on + strand, within LRK54_RS04590 at 977.254 kb on - strand, within LRK54_RS04590 at 977.254 kb on - strand, within LRK54_RS04590
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with SpecU metal mix 1 X remove 976,027 + LRK54_RS04585 0.20 -1.3 976,039 + LRK54_RS04585 0.21 -1.3 976,039 + LRK54_RS04585 0.21 -1.3 976,040 - LRK54_RS04585 0.21 -3.2 976,120 + LRK54_RS04585 0.30 -3.1 976,120 + LRK54_RS04585 0.30 -1.6 976,121 - LRK54_RS04585 0.30 -1.8 976,121 - LRK54_RS04585 0.30 -0.3 976,168 + LRK54_RS04585 0.35 -2.4 976,168 + LRK54_RS04585 0.35 +0.7 976,168 + LRK54_RS04585 0.35 -2.1 976,168 + LRK54_RS04585 0.35 -1.6 976,168 + LRK54_RS04585 0.35 -1.6 976,168 + LRK54_RS04585 0.35 +0.7 976,168 + LRK54_RS04585 0.35 +0.7 976,169 - LRK54_RS04585 0.35 -1.3 976,169 - LRK54_RS04585 0.35 -0.3 976,169 - LRK54_RS04585 0.35 -1.6 976,169 - LRK54_RS04585 0.35 -1.6 976,169 - LRK54_RS04585 0.35 -1.3 976,169 - LRK54_RS04585 0.35 +0.7 976,169 - LRK54_RS04585 0.35 -1.6 976,169 - LRK54_RS04585 0.35 -0.3 976,213 + LRK54_RS04585 0.39 -1.3 976,213 + LRK54_RS04585 0.39 -1.4 976,213 + LRK54_RS04585 0.39 +0.7 976,213 + LRK54_RS04585 0.39 -0.6 976,214 - LRK54_RS04585 0.40 -0.8 976,214 - LRK54_RS04585 0.40 +0.7 976,214 - LRK54_RS04585 0.40 -1.6 976,214 - LRK54_RS04585 0.40 -1.4 976,214 - LRK54_RS04585 0.40 -2.0 976,215 - LRK54_RS04585 0.40 -1.3 976,481 + LRK54_RS04585 0.68 -1.3 976,516 + LRK54_RS04585 0.72 -1.3 976,516 + LRK54_RS04585 0.72 -1.3 976,516 + LRK54_RS04585 0.72 -0.8 976,517 - LRK54_RS04585 0.72 +0.7 976,517 - LRK54_RS04585 0.72 -0.3 976,643 - LRK54_RS04585 0.85 -2.4 976,813 + +0.2 976,813 + -1.3 976,813 + +0.6 976,813 + -1.5 976,814 - +0.7 976,814 - +1.3 976,814 - -0.3 976,875 + -0.7 976,875 + +0.1 976,875 + -0.0 976,875 + +0.5 976,875 + +0.2 976,875 + -1.3 976,875 + -0.0 976,875 + -0.6 976,876 - +0.7 976,876 - +0.1 976,876 - +0.3 976,876 - -0.3 976,876 - +1.7 976,876 - +1.1 976,876 - +2.3 976,876 - +0.2 976,876 - +0.1 976,876 - +0.7 976,876 - +0.4 976,876 - -0.3 976,876 - +0.3 976,876 - +0.6 976,876 - +0.2 976,876 - +1.1 976,876 - +0.1 976,876 - -1.2 977,055 - LRK54_RS04590 0.17 +0.3 977,253 + LRK54_RS04590 0.35 -0.8 977,253 + LRK54_RS04590 0.35 -0.8 977,253 + LRK54_RS04590 0.35 -0.5 977,253 + LRK54_RS04590 0.35 +1.2 977,254 - LRK54_RS04590 0.35 -1.0 977,254 - LRK54_RS04590 0.35 -1.4
Or see this region's nucleotide sequence