Experiment: R2A with SpecU metal mix 1 X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK54_RS02450 and LRK54_RS02455 are separated by 109 nucleotides LRK54_RS02455 and LRK54_RS02460 overlap by 4 nucleotides LRK54_RS02460 and LRK54_RS02465 overlap by 4 nucleotides LRK54_RS02465 and LRK54_RS02470 are separated by 92 nucleotides
LRK54_RS02450: LRK54_RS02450 - GTP cyclohydrolase FolE2, at 532,432 to 533,379
_RS02450
LRK54_RS02455: LRK54_RS02455 - squalene/phytoene synthase family protein, at 533,489 to 534,343
_RS02455
LRK54_RS02460: LRK54_RS02460 - phosphoglycolate phosphatase, at 534,340 to 535,020
_RS02460
LRK54_RS02465: LRK54_RS02465 - bifunctional 2-polyprenyl-6-hydroxyphenol methylase/3-demethylubiquinol 3-O-methyltransferase UbiG, at 535,017 to 535,721
_RS02465
LRK54_RS02470: LRK54_RS02470 - YciI family protein, at 535,814 to 536,068
_RS02470
Position (kb) 534
535
536 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 533.453 kb on + strand at 533.453 kb on + strand at 533.453 kb on + strand at 533.453 kb on + strand at 533.453 kb on + strand at 533.453 kb on + strand at 533.453 kb on + strand at 533.454 kb on - strand at 533.454 kb on - strand at 533.454 kb on - strand at 533.454 kb on - strand at 533.454 kb on - strand at 533.454 kb on - strand at 533.454 kb on - strand at 533.454 kb on - strand at 533.490 kb on + strand at 533.490 kb on + strand at 533.490 kb on + strand at 533.490 kb on + strand at 533.490 kb on + strand at 533.490 kb on + strand at 533.491 kb on - strand at 533.491 kb on - strand at 533.491 kb on - strand at 533.499 kb on + strand at 533.499 kb on + strand at 533.499 kb on + strand at 533.500 kb on - strand at 533.500 kb on - strand at 533.500 kb on - strand at 533.500 kb on - strand at 533.500 kb on - strand at 533.500 kb on - strand at 533.500 kb on - strand at 533.500 kb on - strand at 533.500 kb on - strand at 534.440 kb on + strand, within LRK54_RS02460 at 534.441 kb on - strand, within LRK54_RS02460 at 534.441 kb on - strand, within LRK54_RS02460 at 534.441 kb on - strand, within LRK54_RS02460 at 534.441 kb on - strand, within LRK54_RS02460 at 534.441 kb on - strand, within LRK54_RS02460 at 534.441 kb on - strand, within LRK54_RS02460 at 534.464 kb on + strand, within LRK54_RS02460 at 534.464 kb on + strand, within LRK54_RS02460 at 534.464 kb on + strand, within LRK54_RS02460 at 534.464 kb on + strand, within LRK54_RS02460 at 534.464 kb on + strand, within LRK54_RS02460 at 534.464 kb on + strand, within LRK54_RS02460 at 534.465 kb on + strand, within LRK54_RS02460 at 534.465 kb on - strand, within LRK54_RS02460 at 534.465 kb on - strand, within LRK54_RS02460 at 534.465 kb on - strand, within LRK54_RS02460 at 534.765 kb on + strand, within LRK54_RS02460 at 534.765 kb on + strand, within LRK54_RS02460 at 534.765 kb on + strand, within LRK54_RS02460 at 534.765 kb on + strand, within LRK54_RS02460 at 534.765 kb on + strand, within LRK54_RS02460 at 534.765 kb on + strand, within LRK54_RS02460 at 534.765 kb on + strand, within LRK54_RS02460 at 534.765 kb on + strand, within LRK54_RS02460 at 534.766 kb on - strand, within LRK54_RS02460 at 534.766 kb on - strand, within LRK54_RS02460 at 534.766 kb on - strand, within LRK54_RS02460 at 534.766 kb on - strand, within LRK54_RS02460 at 534.766 kb on - strand, within LRK54_RS02460 at 534.766 kb on - strand, within LRK54_RS02460 at 534.766 kb on - strand, within LRK54_RS02460 at 534.766 kb on - strand, within LRK54_RS02460 at 534.776 kb on + strand, within LRK54_RS02460 at 534.776 kb on + strand, within LRK54_RS02460 at 534.776 kb on + strand, within LRK54_RS02460 at 534.776 kb on + strand, within LRK54_RS02460 at 534.776 kb on + strand, within LRK54_RS02460 at 534.776 kb on + strand, within LRK54_RS02460 at 534.776 kb on + strand, within LRK54_RS02460 at 534.776 kb on + strand, within LRK54_RS02460 at 534.776 kb on + strand, within LRK54_RS02460 at 534.777 kb on - strand, within LRK54_RS02460 at 534.777 kb on - strand, within LRK54_RS02460 at 534.777 kb on - strand, within LRK54_RS02460 at 534.777 kb on - strand, within LRK54_RS02460 at 534.777 kb on - strand, within LRK54_RS02460 at 534.777 kb on - strand, within LRK54_RS02460 at 534.777 kb on - strand, within LRK54_RS02460 at 534.777 kb on - strand, within LRK54_RS02460 at 534.777 kb on - strand, within LRK54_RS02460 at 534.777 kb on - strand, within LRK54_RS02460 at 534.777 kb on - strand, within LRK54_RS02460 at 534.777 kb on - strand, within LRK54_RS02460 at 534.777 kb on - strand, within LRK54_RS02460 at 534.777 kb on - strand, within LRK54_RS02460 at 534.777 kb on - strand, within LRK54_RS02460 at 534.777 kb on - strand, within LRK54_RS02460 at 534.788 kb on + strand, within LRK54_RS02460 at 534.788 kb on + strand, within LRK54_RS02460 at 534.788 kb on + strand, within LRK54_RS02460 at 534.788 kb on + strand, within LRK54_RS02460 at 534.788 kb on + strand, within LRK54_RS02460 at 534.789 kb on - strand, within LRK54_RS02460 at 534.789 kb on - strand, within LRK54_RS02460 at 534.789 kb on - strand, within LRK54_RS02460 at 534.789 kb on - strand, within LRK54_RS02460 at 534.789 kb on - strand, within LRK54_RS02460 at 534.789 kb on - strand, within LRK54_RS02460 at 534.790 kb on - strand, within LRK54_RS02460 at 534.796 kb on + strand, within LRK54_RS02460 at 534.797 kb on - strand, within LRK54_RS02460 at 534.797 kb on - strand, within LRK54_RS02460 at 534.797 kb on - strand, within LRK54_RS02460 at 534.890 kb on + strand, within LRK54_RS02460 at 534.890 kb on + strand, within LRK54_RS02460 at 534.890 kb on + strand, within LRK54_RS02460 at 534.891 kb on - strand, within LRK54_RS02460 at 534.891 kb on - strand, within LRK54_RS02460 at 534.891 kb on - strand, within LRK54_RS02460 at 534.891 kb on - strand, within LRK54_RS02460 at 534.941 kb on + strand, within LRK54_RS02460 at 534.941 kb on + strand, within LRK54_RS02460 at 534.941 kb on + strand, within LRK54_RS02460 at 534.942 kb on - strand, within LRK54_RS02460 at 534.942 kb on - strand, within LRK54_RS02460 at 534.942 kb on - strand, within LRK54_RS02460 at 534.942 kb on - strand, within LRK54_RS02460 at 534.942 kb on - strand, within LRK54_RS02460 at 534.942 kb on - strand, within LRK54_RS02460 at 534.942 kb on - strand, within LRK54_RS02460 at 535.750 kb on + strand at 535.750 kb on + strand at 535.751 kb on - strand at 535.833 kb on + strand at 535.833 kb on + strand at 535.833 kb on + strand at 535.833 kb on + strand at 535.833 kb on + strand at 535.833 kb on + strand at 535.833 kb on + strand at 535.833 kb on + strand at 535.833 kb on + strand at 535.833 kb on + strand at 535.833 kb on + strand at 535.833 kb on + strand at 535.833 kb on + strand at 535.833 kb on + strand at 535.834 kb on - strand at 535.834 kb on - strand at 535.834 kb on - strand at 535.834 kb on - strand at 535.834 kb on - strand at 535.834 kb on - strand at 535.834 kb on - strand at 535.834 kb on - strand at 535.834 kb on - strand at 535.834 kb on - strand at 535.834 kb on - strand at 535.834 kb on - strand at 535.834 kb on - strand at 535.834 kb on - strand at 535.834 kb on - strand at 535.834 kb on - strand at 535.834 kb on - strand at 535.834 kb on - strand at 535.834 kb on - strand at 535.923 kb on + strand, within LRK54_RS02470 at 535.923 kb on + strand, within LRK54_RS02470 at 535.923 kb on + strand, within LRK54_RS02470 at 535.923 kb on + strand, within LRK54_RS02470 at 535.923 kb on + strand, within LRK54_RS02470 at 535.924 kb on - strand, within LRK54_RS02470 at 535.924 kb on - strand, within LRK54_RS02470 at 535.924 kb on - strand, within LRK54_RS02470 at 535.924 kb on - strand, within LRK54_RS02470 at 535.924 kb on - strand, within LRK54_RS02470 at 535.924 kb on - strand, within LRK54_RS02470 at 535.924 kb on - strand, within LRK54_RS02470 at 535.924 kb on - strand, within LRK54_RS02470 at 535.924 kb on - strand, within LRK54_RS02470
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with SpecU metal mix 1 X remove 533,453 + +0.1 533,453 + +0.5 533,453 + -0.5 533,453 + +0.7 533,453 + +1.7 533,453 + +0.0 533,453 + +3.3 533,454 - +1.7 533,454 - -0.8 533,454 - -0.9 533,454 - +0.1 533,454 - +0.9 533,454 - -1.9 533,454 - +0.7 533,454 - -0.7 533,490 + +0.9 533,490 + +0.6 533,490 + -0.0 533,490 + +0.4 533,490 + +0.2 533,490 + +1.4 533,491 - -0.5 533,491 - +1.3 533,491 - +0.7 533,499 + -0.8 533,499 + -0.6 533,499 + -0.3 533,500 - +0.1 533,500 - +0.9 533,500 - -0.6 533,500 - +0.3 533,500 - -0.3 533,500 - -1.1 533,500 - -0.5 533,500 - -2.2 533,500 - -0.0 534,440 + LRK54_RS02460 0.15 -0.3 534,441 - LRK54_RS02460 0.15 -1.6 534,441 - LRK54_RS02460 0.15 -2.4 534,441 - LRK54_RS02460 0.15 -3.0 534,441 - LRK54_RS02460 0.15 -1.2 534,441 - LRK54_RS02460 0.15 -2.3 534,441 - LRK54_RS02460 0.15 -1.3 534,464 + LRK54_RS02460 0.18 -0.9 534,464 + LRK54_RS02460 0.18 -1.6 534,464 + LRK54_RS02460 0.18 -0.6 534,464 + LRK54_RS02460 0.18 -0.9 534,464 + LRK54_RS02460 0.18 -0.3 534,464 + LRK54_RS02460 0.18 -1.3 534,465 + LRK54_RS02460 0.18 -3.1 534,465 - LRK54_RS02460 0.18 -0.9 534,465 - LRK54_RS02460 0.18 +0.7 534,465 - LRK54_RS02460 0.18 -0.3 534,765 + LRK54_RS02460 0.62 -1.3 534,765 + LRK54_RS02460 0.62 -3.2 534,765 + LRK54_RS02460 0.62 -0.9 534,765 + LRK54_RS02460 0.62 +0.7 534,765 + LRK54_RS02460 0.62 -1.9 534,765 + LRK54_RS02460 0.62 -1.3 534,765 + LRK54_RS02460 0.62 +0.7 534,765 + LRK54_RS02460 0.62 -1.5 534,766 - LRK54_RS02460 0.63 -1.3 534,766 - LRK54_RS02460 0.63 -2.0 534,766 - LRK54_RS02460 0.63 -1.5 534,766 - LRK54_RS02460 0.63 -0.5 534,766 - LRK54_RS02460 0.63 -1.9 534,766 - LRK54_RS02460 0.63 -1.3 534,766 - LRK54_RS02460 0.63 -0.3 534,766 - LRK54_RS02460 0.63 -1.8 534,776 + LRK54_RS02460 0.64 +1.7 534,776 + LRK54_RS02460 0.64 +1.7 534,776 + LRK54_RS02460 0.64 -2.3 534,776 + LRK54_RS02460 0.64 -1.3 534,776 + LRK54_RS02460 0.64 -1.9 534,776 + LRK54_RS02460 0.64 +1.1 534,776 + LRK54_RS02460 0.64 -1.1 534,776 + LRK54_RS02460 0.64 -0.1 534,776 + LRK54_RS02460 0.64 +0.7 534,777 - LRK54_RS02460 0.64 -0.7 534,777 - LRK54_RS02460 0.64 -1.3 534,777 - LRK54_RS02460 0.64 -2.4 534,777 - LRK54_RS02460 0.64 -2.8 534,777 - LRK54_RS02460 0.64 -1.7 534,777 - LRK54_RS02460 0.64 +1.4 534,777 - LRK54_RS02460 0.64 +0.7 534,777 - LRK54_RS02460 0.64 -2.5 534,777 - LRK54_RS02460 0.64 -1.9 534,777 - LRK54_RS02460 0.64 -1.3 534,777 - LRK54_RS02460 0.64 -1.3 534,777 - LRK54_RS02460 0.64 -0.7 534,777 - LRK54_RS02460 0.64 -1.3 534,777 - LRK54_RS02460 0.64 -1.1 534,777 - LRK54_RS02460 0.64 -1.9 534,777 - LRK54_RS02460 0.64 -2.8 534,788 + LRK54_RS02460 0.66 -1.9 534,788 + LRK54_RS02460 0.66 -1.3 534,788 + LRK54_RS02460 0.66 -1.7 534,788 + LRK54_RS02460 0.66 +0.7 534,788 + LRK54_RS02460 0.66 -1.3 534,789 - LRK54_RS02460 0.66 -1.3 534,789 - LRK54_RS02460 0.66 -0.9 534,789 - LRK54_RS02460 0.66 +1.3 534,789 - LRK54_RS02460 0.66 -2.9 534,789 - LRK54_RS02460 0.66 -2.4 534,789 - LRK54_RS02460 0.66 -2.5 534,790 - LRK54_RS02460 0.66 +0.1 534,796 + LRK54_RS02460 0.67 -2.3 534,797 - LRK54_RS02460 0.67 -1.1 534,797 - LRK54_RS02460 0.67 -1.9 534,797 - LRK54_RS02460 0.67 +0.7 534,890 + LRK54_RS02460 0.81 -1.6 534,890 + LRK54_RS02460 0.81 -2.6 534,890 + LRK54_RS02460 0.81 -0.9 534,891 - LRK54_RS02460 0.81 -0.8 534,891 - LRK54_RS02460 0.81 -1.6 534,891 - LRK54_RS02460 0.81 -3.1 534,891 - LRK54_RS02460 0.81 -1.5 534,941 + LRK54_RS02460 0.88 +0.1 534,941 + LRK54_RS02460 0.88 -0.9 534,941 + LRK54_RS02460 0.88 -3.6 534,942 - LRK54_RS02460 0.88 -2.5 534,942 - LRK54_RS02460 0.88 -2.5 534,942 - LRK54_RS02460 0.88 -2.0 534,942 - LRK54_RS02460 0.88 -1.6 534,942 - LRK54_RS02460 0.88 -0.1 534,942 - LRK54_RS02460 0.88 -1.3 534,942 - LRK54_RS02460 0.88 -2.5 535,750 + +1.1 535,750 + +0.4 535,751 - +1.0 535,833 + -0.0 535,833 + -0.3 535,833 + +0.7 535,833 + -0.5 535,833 + +0.7 535,833 + -0.9 535,833 + -0.5 535,833 + +0.1 535,833 + -1.5 535,833 + +0.2 535,833 + -0.0 535,833 + -1.1 535,833 + +0.2 535,833 + +0.9 535,834 - -0.9 535,834 - +0.4 535,834 - -0.4 535,834 - -1.6 535,834 - -0.0 535,834 - +2.3 535,834 - +0.3 535,834 - +1.0 535,834 - -2.6 535,834 - -0.1 535,834 - +0.0 535,834 - +0.7 535,834 - -0.7 535,834 - +0.7 535,834 - -2.0 535,834 - -0.2 535,834 - +0.0 535,834 - +1.0 535,834 - +0.8 535,923 + LRK54_RS02470 0.43 +0.2 535,923 + LRK54_RS02470 0.43 -0.6 535,923 + LRK54_RS02470 0.43 -0.8 535,923 + LRK54_RS02470 0.43 -1.1 535,923 + LRK54_RS02470 0.43 -0.1 535,924 - LRK54_RS02470 0.43 -0.1 535,924 - LRK54_RS02470 0.43 +1.1 535,924 - LRK54_RS02470 0.43 -0.3 535,924 - LRK54_RS02470 0.43 +0.7 535,924 - LRK54_RS02470 0.43 +0.4 535,924 - LRK54_RS02470 0.43 -0.2 535,924 - LRK54_RS02470 0.43 -1.4 535,924 - LRK54_RS02470 0.43 -0.2 535,924 - LRK54_RS02470 0.43 -2.3
Or see this region's nucleotide sequence