Experiment: R2A with SpecU metal mix 1 X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK54_RS02440 and LRK54_RS02445 are separated by 145 nucleotides LRK54_RS02445 and LRK54_RS02450 are separated by 120 nucleotides
LRK54_RS02440: LRK54_RS02440 - RNA polymerase-binding protein DksA, at 530,679 to 531,923
_RS02440
LRK54_RS02445: LRK54_RS02445 - membrane protein insertion efficiency factor YidD, at 532,069 to 532,311
_RS02445
LRK54_RS02450: LRK54_RS02450 - GTP cyclohydrolase FolE2, at 532,432 to 533,379
_RS02450
Position (kb)
532
533 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 531.162 kb on - strand, within LRK54_RS02440 at 531.300 kb on + strand, within LRK54_RS02440 at 531.301 kb on - strand, within LRK54_RS02440 at 531.565 kb on - strand, within LRK54_RS02440 at 531.565 kb on - strand, within LRK54_RS02440 at 531.905 kb on + strand at 531.905 kb on + strand at 531.905 kb on + strand at 531.905 kb on + strand at 531.905 kb on + strand at 531.905 kb on + strand at 531.905 kb on + strand at 531.905 kb on + strand at 531.906 kb on - strand at 531.906 kb on - strand at 531.906 kb on - strand at 531.906 kb on - strand at 531.906 kb on - strand at 531.906 kb on - strand at 531.906 kb on - strand at 531.906 kb on - strand at 531.906 kb on - strand at 531.906 kb on - strand at 531.906 kb on - strand at 531.906 kb on - strand at 531.906 kb on - strand at 531.906 kb on - strand at 531.906 kb on - strand at 531.906 kb on - strand at 531.906 kb on - strand at 531.924 kb on - strand at 531.924 kb on - strand at 531.924 kb on - strand at 531.924 kb on - strand at 531.924 kb on - strand at 531.924 kb on - strand at 531.960 kb on + strand at 531.960 kb on + strand at 531.960 kb on + strand at 531.960 kb on + strand at 531.960 kb on + strand at 531.961 kb on - strand at 531.961 kb on - strand at 531.961 kb on - strand at 531.961 kb on - strand at 531.961 kb on - strand at 531.961 kb on - strand at 531.961 kb on - strand at 531.961 kb on - strand at 531.961 kb on - strand at 531.961 kb on - strand at 531.961 kb on - strand at 531.962 kb on + strand at 531.962 kb on + strand at 531.962 kb on + strand at 531.962 kb on + strand at 531.962 kb on + strand at 531.962 kb on + strand at 531.963 kb on - strand at 531.963 kb on - strand at 531.963 kb on - strand at 531.963 kb on - strand at 531.963 kb on - strand at 531.963 kb on - strand at 531.963 kb on - strand at 531.963 kb on - strand at 531.963 kb on - strand at 531.963 kb on - strand at 531.963 kb on - strand at 531.963 kb on - strand at 531.976 kb on + strand at 531.977 kb on - strand at 531.977 kb on - strand at 532.001 kb on + strand at 532.001 kb on + strand at 532.001 kb on + strand at 532.001 kb on + strand at 532.001 kb on + strand at 532.001 kb on + strand at 532.001 kb on + strand at 532.001 kb on + strand at 532.001 kb on + strand at 532.001 kb on + strand at 532.001 kb on + strand at 532.001 kb on + strand at 532.001 kb on + strand at 532.001 kb on + strand at 532.001 kb on + strand at 532.001 kb on + strand at 532.001 kb on + strand at 532.001 kb on + strand at 532.001 kb on + strand at 532.001 kb on + strand at 532.002 kb on - strand at 532.002 kb on - strand at 532.002 kb on - strand at 532.002 kb on - strand at 532.002 kb on - strand at 532.002 kb on - strand at 532.002 kb on - strand at 532.002 kb on - strand at 532.002 kb on - strand at 532.002 kb on - strand at 532.002 kb on - strand at 532.002 kb on - strand at 532.007 kb on + strand at 532.007 kb on + strand at 532.007 kb on + strand at 532.007 kb on + strand at 532.007 kb on + strand at 532.007 kb on + strand at 532.007 kb on + strand at 532.007 kb on + strand at 532.007 kb on + strand at 532.007 kb on + strand at 532.007 kb on + strand at 532.007 kb on + strand at 532.007 kb on + strand at 532.007 kb on + strand at 532.007 kb on + strand at 532.007 kb on + strand at 532.007 kb on + strand at 532.007 kb on + strand at 532.007 kb on + strand at 532.007 kb on + strand at 532.007 kb on + strand at 532.007 kb on + strand at 532.007 kb on + strand at 532.008 kb on - strand at 532.008 kb on - strand at 532.008 kb on - strand at 532.008 kb on - strand at 532.008 kb on - strand at 532.008 kb on - strand at 532.008 kb on - strand at 532.008 kb on - strand at 532.008 kb on - strand at 532.008 kb on - strand at 532.008 kb on - strand at 532.008 kb on - strand at 532.008 kb on - strand at 532.008 kb on - strand at 532.008 kb on - strand at 532.008 kb on - strand at 532.008 kb on - strand at 532.008 kb on - strand at 532.008 kb on - strand at 532.008 kb on - strand at 532.008 kb on - strand at 532.008 kb on - strand at 532.008 kb on - strand at 532.008 kb on - strand at 532.008 kb on - strand at 532.013 kb on + strand at 532.013 kb on + strand at 532.013 kb on + strand at 532.013 kb on + strand at 532.013 kb on + strand at 532.013 kb on + strand at 532.013 kb on + strand at 532.013 kb on + strand at 532.014 kb on - strand at 532.014 kb on - strand at 532.014 kb on - strand at 532.014 kb on - strand at 532.014 kb on - strand at 532.014 kb on - strand at 532.014 kb on - strand at 532.014 kb on - strand at 532.014 kb on - strand at 532.014 kb on - strand at 532.061 kb on + strand at 532.061 kb on + strand at 532.061 kb on + strand at 532.061 kb on + strand at 532.061 kb on + strand at 532.061 kb on + strand at 532.061 kb on + strand at 532.061 kb on + strand at 532.061 kb on + strand at 532.061 kb on + strand at 532.062 kb on - strand at 532.062 kb on - strand at 532.062 kb on - strand at 532.062 kb on - strand at 532.062 kb on - strand at 532.062 kb on - strand at 532.062 kb on - strand at 532.062 kb on - strand at 532.062 kb on - strand at 532.062 kb on - strand at 532.062 kb on - strand at 532.062 kb on - strand at 532.062 kb on - strand at 532.062 kb on - strand at 532.081 kb on + strand at 532.081 kb on + strand at 532.081 kb on + strand at 532.081 kb on + strand at 532.081 kb on + strand at 532.081 kb on + strand at 532.081 kb on + strand at 532.081 kb on + strand at 532.081 kb on + strand at 532.081 kb on + strand at 532.082 kb on - strand at 532.082 kb on - strand at 532.082 kb on - strand at 532.082 kb on - strand at 532.082 kb on - strand at 532.082 kb on - strand at 532.082 kb on - strand at 532.082 kb on - strand at 532.082 kb on - strand at 532.082 kb on - strand at 532.144 kb on + strand, within LRK54_RS02445 at 532.144 kb on + strand, within LRK54_RS02445 at 532.144 kb on + strand, within LRK54_RS02445 at 532.144 kb on + strand, within LRK54_RS02445 at 532.144 kb on + strand, within LRK54_RS02445 at 532.144 kb on + strand, within LRK54_RS02445 at 532.145 kb on - strand, within LRK54_RS02445 at 532.379 kb on + strand at 532.379 kb on + strand at 532.379 kb on + strand at 532.380 kb on - strand at 532.380 kb on - strand at 532.380 kb on - strand at 532.380 kb on - strand at 532.448 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with SpecU metal mix 1 X remove 531,162 - LRK54_RS02440 0.39 -1.9 531,300 + LRK54_RS02440 0.50 -4.2 531,301 - LRK54_RS02440 0.50 -0.9 531,565 - LRK54_RS02440 0.71 -2.5 531,565 - LRK54_RS02440 0.71 -0.3 531,905 + -0.3 531,905 + -0.9 531,905 + -0.3 531,905 + -0.3 531,905 + +0.7 531,905 + -2.5 531,905 + -2.1 531,905 + -2.6 531,906 - -2.9 531,906 - -3.0 531,906 - -0.3 531,906 - -0.9 531,906 - -1.9 531,906 - -2.6 531,906 - -3.8 531,906 - -3.9 531,906 - -1.3 531,906 - -2.3 531,906 - -1.5 531,906 - -3.6 531,906 - -0.3 531,906 - -2.7 531,906 - +0.7 531,906 - -2.9 531,906 - -2.9 531,924 - -0.3 531,924 - -3.7 531,924 - -1.6 531,924 - -0.9 531,924 - -0.9 531,924 - -2.7 531,960 + +0.3 531,960 + -3.2 531,960 + -0.3 531,960 + -0.3 531,960 + -2.5 531,961 - -2.1 531,961 - -0.8 531,961 - -0.7 531,961 - -2.6 531,961 - -0.9 531,961 - +0.7 531,961 - -2.5 531,961 - -1.2 531,961 - -1.2 531,961 - -1.1 531,961 - -1.1 531,962 + -1.9 531,962 + -2.3 531,962 + -1.9 531,962 + -2.1 531,962 + -1.7 531,962 + +0.7 531,963 - -2.5 531,963 - +0.1 531,963 - -0.8 531,963 - -0.2 531,963 - -3.2 531,963 - -3.0 531,963 - +0.5 531,963 - -0.7 531,963 - -2.8 531,963 - -1.5 531,963 - -1.5 531,963 - -0.9 531,976 + -2.8 531,977 - -2.3 531,977 - -2.0 532,001 + -1.1 532,001 + -0.3 532,001 + +0.2 532,001 + -0.3 532,001 + -0.6 532,001 + -0.3 532,001 + -1.1 532,001 + -0.2 532,001 + -1.3 532,001 + -0.6 532,001 + +0.2 532,001 + -0.3 532,001 + -1.6 532,001 + -0.8 532,001 + +2.1 532,001 + -2.5 532,001 + -0.5 532,001 + -0.2 532,001 + -0.8 532,001 + +0.3 532,002 - +0.7 532,002 - -0.5 532,002 - -2.6 532,002 - -0.3 532,002 - -0.1 532,002 - -0.5 532,002 - +0.4 532,002 - +1.2 532,002 - +0.3 532,002 - -0.0 532,002 - +1.0 532,002 - -2.6 532,007 + -0.9 532,007 + -1.0 532,007 + +0.7 532,007 + -0.3 532,007 + +1.5 532,007 + +1.3 532,007 + -0.2 532,007 + +0.4 532,007 + +0.4 532,007 + -2.3 532,007 + -0.3 532,007 + +0.4 532,007 + -0.5 532,007 + -2.0 532,007 + -1.1 532,007 + -1.7 532,007 + +1.3 532,007 + +0.3 532,007 + -0.9 532,007 + +0.2 532,007 + -1.7 532,007 + -0.6 532,007 + -1.2 532,008 - +0.1 532,008 - +0.0 532,008 - +0.4 532,008 - -0.6 532,008 - -1.2 532,008 - +0.3 532,008 - +0.5 532,008 - -0.5 532,008 - -0.1 532,008 - +0.6 532,008 - +0.3 532,008 - +0.4 532,008 - +1.3 532,008 - +0.7 532,008 - -0.1 532,008 - +0.7 532,008 - +0.3 532,008 - +0.9 532,008 - +0.7 532,008 - -0.8 532,008 - -1.5 532,008 - -0.9 532,008 - +0.7 532,008 - -1.1 532,008 - -2.3 532,013 + -0.6 532,013 + +0.4 532,013 + -0.5 532,013 + +0.8 532,013 + -0.9 532,013 + -1.1 532,013 + -0.5 532,013 + +0.5 532,014 - -0.3 532,014 - +0.0 532,014 - -1.3 532,014 - -0.6 532,014 - -0.3 532,014 - -0.6 532,014 - +1.3 532,014 - -0.5 532,014 - +1.6 532,014 - +0.9 532,061 + -0.3 532,061 + -1.6 532,061 + -1.1 532,061 + -0.4 532,061 + +0.0 532,061 + -2.6 532,061 + +0.7 532,061 + -1.1 532,061 + -1.9 532,061 + -0.3 532,062 - -0.6 532,062 - +1.3 532,062 - +0.1 532,062 - +0.1 532,062 - -0.1 532,062 - -2.5 532,062 - +0.1 532,062 - +0.9 532,062 - +0.1 532,062 - +0.3 532,062 - +0.7 532,062 - -2.5 532,062 - -0.1 532,062 - +0.5 532,081 + -1.6 532,081 + -2.1 532,081 + -3.3 532,081 + -1.3 532,081 + -2.7 532,081 + -0.1 532,081 + -1.1 532,081 + -1.9 532,081 + -1.1 532,081 + -0.9 532,082 - -2.3 532,082 - -0.3 532,082 - -1.3 532,082 - -0.3 532,082 - -2.3 532,082 - -0.3 532,082 - -0.3 532,082 - -0.9 532,082 - -0.6 532,082 - -2.3 532,144 + LRK54_RS02445 0.31 -1.6 532,144 + LRK54_RS02445 0.31 -1.3 532,144 + LRK54_RS02445 0.31 -1.9 532,144 + LRK54_RS02445 0.31 -2.9 532,144 + LRK54_RS02445 0.31 -0.2 532,144 + LRK54_RS02445 0.31 -2.5 532,145 - LRK54_RS02445 0.31 -0.6 532,379 + +3.3 532,379 + +1.7 532,379 + +0.4 532,380 - +1.7 532,380 - -0.0 532,380 - +1.4 532,380 - +0.6 532,448 + +0.9
Or see this region's nucleotide sequence