Experiment: R2A with SpecU metal mix 1 X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK54_RS01575 and LRK54_RS01580 are separated by 213 nucleotides LRK54_RS01580 and LRK54_RS01585 overlap by 7 nucleotides LRK54_RS01585 and LRK54_RS01590 overlap by 4 nucleotides LRK54_RS01590 and LRK54_RS01595 are separated by 109 nucleotides
LRK54_RS01575: LRK54_RS01575 - bifunctional phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE, at 340,553 to 341,182
_RS01575
LRK54_RS01580: LRK54_RS01580 - imidazole glycerol phosphate synthase subunit HisF, at 341,396 to 342,169
_RS01580
LRK54_RS01585: LRK54_RS01585 - 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino]imidazole-4- carboxamide isomerase, at 342,163 to 342,891
_RS01585
LRK54_RS01590: LRK54_RS01590 - imidazole glycerol phosphate synthase subunit HisH, at 342,888 to 343,478
_RS01590
LRK54_RS01595: LRK54_RS01595 - bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB, at 343,588 to 344,652
_RS01595
Position (kb) 342
343 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 341.187 kb on + strand at 341.187 kb on + strand at 341.187 kb on + strand at 341.188 kb on - strand at 341.188 kb on - strand at 341.188 kb on - strand at 341.433 kb on + strand at 341.433 kb on + strand at 341.433 kb on + strand at 341.433 kb on + strand at 341.433 kb on + strand at 341.433 kb on + strand at 341.433 kb on + strand at 341.433 kb on + strand at 341.433 kb on + strand at 341.434 kb on - strand at 341.434 kb on - strand at 341.434 kb on - strand at 341.434 kb on - strand at 341.610 kb on + strand, within LRK54_RS01580 at 341.739 kb on + strand, within LRK54_RS01580 at 341.739 kb on + strand, within LRK54_RS01580 at 341.739 kb on + strand, within LRK54_RS01580 at 341.739 kb on + strand, within LRK54_RS01580 at 341.740 kb on - strand, within LRK54_RS01580 at 342.021 kb on + strand, within LRK54_RS01580 at 342.021 kb on + strand, within LRK54_RS01580 at 342.021 kb on + strand, within LRK54_RS01580 at 342.021 kb on + strand, within LRK54_RS01580 at 342.021 kb on + strand, within LRK54_RS01580 at 342.021 kb on + strand, within LRK54_RS01580 at 342.021 kb on + strand, within LRK54_RS01580 at 342.021 kb on + strand, within LRK54_RS01580 at 342.021 kb on + strand, within LRK54_RS01580 at 342.021 kb on + strand, within LRK54_RS01580 at 342.021 kb on + strand, within LRK54_RS01580 at 342.021 kb on + strand, within LRK54_RS01580 at 342.021 kb on + strand, within LRK54_RS01580 at 342.021 kb on + strand, within LRK54_RS01580 at 342.022 kb on - strand, within LRK54_RS01580 at 342.022 kb on - strand, within LRK54_RS01580 at 342.022 kb on - strand, within LRK54_RS01580 at 342.022 kb on - strand, within LRK54_RS01580 at 342.022 kb on - strand, within LRK54_RS01580 at 342.329 kb on + strand, within LRK54_RS01585 at 342.410 kb on + strand, within LRK54_RS01585 at 342.410 kb on + strand, within LRK54_RS01585 at 342.411 kb on - strand, within LRK54_RS01585 at 342.411 kb on - strand, within LRK54_RS01585 at 342.530 kb on + strand, within LRK54_RS01585 at 342.530 kb on + strand, within LRK54_RS01585 at 342.531 kb on - strand, within LRK54_RS01585 at 342.640 kb on + strand, within LRK54_RS01585 at 342.640 kb on + strand, within LRK54_RS01585 at 342.640 kb on + strand, within LRK54_RS01585 at 342.770 kb on + strand, within LRK54_RS01585 at 342.771 kb on - strand, within LRK54_RS01585 at 342.803 kb on + strand, within LRK54_RS01585 at 342.803 kb on + strand, within LRK54_RS01585 at 342.803 kb on + strand, within LRK54_RS01585 at 342.803 kb on + strand, within LRK54_RS01585 at 342.803 kb on + strand, within LRK54_RS01585 at 342.803 kb on + strand, within LRK54_RS01585 at 342.803 kb on + strand, within LRK54_RS01585 at 342.803 kb on + strand, within LRK54_RS01585 at 342.803 kb on + strand, within LRK54_RS01585 at 342.803 kb on + strand, within LRK54_RS01585 at 342.803 kb on + strand, within LRK54_RS01585 at 342.803 kb on + strand, within LRK54_RS01585 at 342.803 kb on + strand, within LRK54_RS01585 at 342.803 kb on + strand, within LRK54_RS01585 at 342.803 kb on + strand, within LRK54_RS01585 at 342.803 kb on + strand, within LRK54_RS01585 at 342.804 kb on - strand, within LRK54_RS01585 at 342.804 kb on - strand, within LRK54_RS01585 at 342.804 kb on - strand, within LRK54_RS01585 at 342.804 kb on - strand, within LRK54_RS01585 at 342.804 kb on - strand, within LRK54_RS01585 at 342.804 kb on - strand, within LRK54_RS01585 at 342.804 kb on - strand, within LRK54_RS01585 at 342.804 kb on - strand, within LRK54_RS01585 at 342.804 kb on - strand, within LRK54_RS01585 at 342.804 kb on - strand, within LRK54_RS01585 at 342.822 kb on - strand at 342.891 kb on + strand at 342.891 kb on + strand at 342.891 kb on + strand at 342.891 kb on + strand at 342.891 kb on + strand at 342.891 kb on + strand at 342.891 kb on + strand at 342.891 kb on + strand at 342.891 kb on + strand at 342.891 kb on + strand at 342.891 kb on + strand at 342.891 kb on + strand at 342.892 kb on - strand at 342.892 kb on - strand at 342.892 kb on - strand at 342.892 kb on - strand at 342.892 kb on - strand at 342.892 kb on - strand at 342.892 kb on - strand at 342.892 kb on - strand at 342.892 kb on - strand at 342.892 kb on - strand at 342.892 kb on - strand at 343.003 kb on + strand, within LRK54_RS01590 at 343.003 kb on + strand, within LRK54_RS01590 at 343.003 kb on + strand, within LRK54_RS01590 at 343.003 kb on + strand, within LRK54_RS01590 at 343.003 kb on + strand, within LRK54_RS01590 at 343.004 kb on - strand, within LRK54_RS01590 at 343.045 kb on + strand, within LRK54_RS01590 at 343.045 kb on + strand, within LRK54_RS01590 at 343.045 kb on + strand, within LRK54_RS01590 at 343.045 kb on + strand, within LRK54_RS01590 at 343.045 kb on + strand, within LRK54_RS01590 at 343.045 kb on + strand, within LRK54_RS01590 at 343.045 kb on + strand, within LRK54_RS01590 at 343.045 kb on + strand, within LRK54_RS01590 at 343.045 kb on + strand, within LRK54_RS01590 at 343.045 kb on + strand, within LRK54_RS01590 at 343.045 kb on + strand, within LRK54_RS01590 at 343.045 kb on + strand, within LRK54_RS01590 at 343.045 kb on + strand, within LRK54_RS01590 at 343.045 kb on + strand, within LRK54_RS01590 at 343.046 kb on - strand, within LRK54_RS01590 at 343.046 kb on - strand, within LRK54_RS01590 at 343.046 kb on - strand, within LRK54_RS01590 at 343.046 kb on - strand, within LRK54_RS01590 at 343.046 kb on - strand, within LRK54_RS01590 at 343.046 kb on - strand, within LRK54_RS01590 at 343.046 kb on - strand, within LRK54_RS01590 at 343.046 kb on - strand, within LRK54_RS01590 at 343.046 kb on - strand, within LRK54_RS01590 at 343.046 kb on - strand, within LRK54_RS01590 at 343.046 kb on - strand, within LRK54_RS01590 at 343.046 kb on - strand, within LRK54_RS01590 at 343.046 kb on - strand, within LRK54_RS01590 at 343.046 kb on - strand, within LRK54_RS01590 at 343.046 kb on - strand, within LRK54_RS01590 at 343.046 kb on - strand, within LRK54_RS01590 at 343.046 kb on - strand, within LRK54_RS01590 at 343.046 kb on - strand, within LRK54_RS01590 at 343.046 kb on - strand, within LRK54_RS01590 at 343.046 kb on - strand, within LRK54_RS01590 at 343.046 kb on - strand, within LRK54_RS01590 at 343.046 kb on - strand, within LRK54_RS01590 at 343.046 kb on - strand, within LRK54_RS01590 at 343.060 kb on + strand, within LRK54_RS01590 at 343.060 kb on + strand, within LRK54_RS01590 at 343.060 kb on + strand, within LRK54_RS01590 at 343.060 kb on + strand, within LRK54_RS01590 at 343.060 kb on + strand, within LRK54_RS01590 at 343.061 kb on - strand, within LRK54_RS01590 at 343.061 kb on - strand, within LRK54_RS01590 at 343.061 kb on - strand, within LRK54_RS01590 at 343.061 kb on - strand, within LRK54_RS01590 at 343.061 kb on - strand, within LRK54_RS01590 at 343.391 kb on + strand, within LRK54_RS01590 at 343.426 kb on + strand at 343.426 kb on + strand at 343.426 kb on + strand at 343.426 kb on + strand at 343.426 kb on + strand at 343.427 kb on - strand at 343.427 kb on - strand at 343.431 kb on + strand at 343.431 kb on + strand at 343.431 kb on + strand at 343.443 kb on - strand at 343.482 kb on + strand at 343.482 kb on + strand at 343.482 kb on + strand at 343.482 kb on + strand at 343.482 kb on + strand at 343.482 kb on + strand at 343.482 kb on + strand at 343.482 kb on + strand at 343.483 kb on - strand at 343.483 kb on - strand at 343.483 kb on - strand at 343.483 kb on - strand at 343.483 kb on - strand at 343.483 kb on - strand at 343.483 kb on - strand at 343.483 kb on - strand at 343.483 kb on - strand at 343.483 kb on - strand at 343.483 kb on - strand at 343.540 kb on + strand at 343.540 kb on + strand at 343.540 kb on + strand at 343.540 kb on + strand at 343.541 kb on - strand at 343.541 kb on - strand at 343.589 kb on + strand at 343.589 kb on + strand at 343.589 kb on + strand at 343.589 kb on + strand at 343.589 kb on + strand at 343.589 kb on + strand at 343.589 kb on + strand at 343.589 kb on + strand at 343.589 kb on + strand at 343.589 kb on + strand at 343.589 kb on + strand at 343.589 kb on + strand at 343.589 kb on + strand at 343.589 kb on + strand at 343.589 kb on + strand at 343.589 kb on + strand at 343.589 kb on + strand at 343.589 kb on + strand at 343.589 kb on + strand at 343.589 kb on + strand at 343.589 kb on + strand at 343.589 kb on + strand at 343.589 kb on + strand at 343.589 kb on + strand at 343.590 kb on - strand at 343.590 kb on - strand at 343.590 kb on - strand at 343.590 kb on - strand at 343.590 kb on - strand at 343.590 kb on - strand at 343.590 kb on - strand at 343.590 kb on - strand at 343.590 kb on - strand at 343.590 kb on - strand at 343.590 kb on - strand at 343.590 kb on - strand at 343.590 kb on - strand at 343.590 kb on - strand at 343.590 kb on - strand at 343.590 kb on - strand at 343.590 kb on - strand at 343.590 kb on - strand at 343.590 kb on - strand at 343.590 kb on - strand at 343.606 kb on - strand at 343.823 kb on + strand, within LRK54_RS01595 at 343.823 kb on + strand, within LRK54_RS01595 at 343.823 kb on + strand, within LRK54_RS01595 at 343.823 kb on + strand, within LRK54_RS01595 at 343.823 kb on + strand, within LRK54_RS01595 at 343.823 kb on + strand, within LRK54_RS01595 at 343.824 kb on - strand, within LRK54_RS01595
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with SpecU metal mix 1 X remove 341,187 + -0.5 341,187 + -0.7 341,187 + +0.6 341,188 - +0.6 341,188 - +0.2 341,188 - -0.2 341,433 + -2.1 341,433 + +0.7 341,433 + -0.1 341,433 + +0.1 341,433 + -0.3 341,433 + -1.3 341,433 + -2.6 341,433 + +0.7 341,433 + -1.9 341,434 - -3.6 341,434 - -0.9 341,434 - -1.9 341,434 - -1.6 341,610 + LRK54_RS01580 0.28 -2.6 341,739 + LRK54_RS01580 0.44 +0.7 341,739 + LRK54_RS01580 0.44 -0.1 341,739 + LRK54_RS01580 0.44 -0.9 341,739 + LRK54_RS01580 0.44 -2.1 341,740 - LRK54_RS01580 0.44 -0.7 342,021 + LRK54_RS01580 0.81 -0.9 342,021 + LRK54_RS01580 0.81 -2.2 342,021 + LRK54_RS01580 0.81 -2.5 342,021 + LRK54_RS01580 0.81 -2.6 342,021 + LRK54_RS01580 0.81 -1.9 342,021 + LRK54_RS01580 0.81 -1.7 342,021 + LRK54_RS01580 0.81 -1.5 342,021 + LRK54_RS01580 0.81 +0.4 342,021 + LRK54_RS01580 0.81 -1.9 342,021 + LRK54_RS01580 0.81 -3.2 342,021 + LRK54_RS01580 0.81 -1.1 342,021 + LRK54_RS01580 0.81 -2.1 342,021 + LRK54_RS01580 0.81 -1.6 342,021 + LRK54_RS01580 0.81 -1.9 342,022 - LRK54_RS01580 0.81 -0.3 342,022 - LRK54_RS01580 0.81 -0.1 342,022 - LRK54_RS01580 0.81 -1.9 342,022 - LRK54_RS01580 0.81 -1.3 342,022 - LRK54_RS01580 0.81 -2.8 342,329 + LRK54_RS01585 0.23 -1.3 342,410 + LRK54_RS01585 0.34 -0.3 342,410 + LRK54_RS01585 0.34 -0.6 342,411 - LRK54_RS01585 0.34 -2.6 342,411 - LRK54_RS01585 0.34 -1.9 342,530 + LRK54_RS01585 0.50 -3.2 342,530 + LRK54_RS01585 0.50 -0.3 342,531 - LRK54_RS01585 0.50 -2.1 342,640 + LRK54_RS01585 0.65 -1.6 342,640 + LRK54_RS01585 0.65 -1.9 342,640 + LRK54_RS01585 0.65 -2.6 342,770 + LRK54_RS01585 0.83 +0.7 342,771 - LRK54_RS01585 0.83 -0.9 342,803 + LRK54_RS01585 0.88 -0.3 342,803 + LRK54_RS01585 0.88 -0.6 342,803 + LRK54_RS01585 0.88 -1.5 342,803 + LRK54_RS01585 0.88 -0.3 342,803 + LRK54_RS01585 0.88 -1.7 342,803 + LRK54_RS01585 0.88 -2.3 342,803 + LRK54_RS01585 0.88 +0.7 342,803 + LRK54_RS01585 0.88 -3.0 342,803 + LRK54_RS01585 0.88 -0.9 342,803 + LRK54_RS01585 0.88 -1.0 342,803 + LRK54_RS01585 0.88 -2.6 342,803 + LRK54_RS01585 0.88 -0.3 342,803 + LRK54_RS01585 0.88 -1.4 342,803 + LRK54_RS01585 0.88 -1.3 342,803 + LRK54_RS01585 0.88 -1.3 342,803 + LRK54_RS01585 0.88 -3.3 342,804 - LRK54_RS01585 0.88 -0.3 342,804 - LRK54_RS01585 0.88 -2.5 342,804 - LRK54_RS01585 0.88 -1.5 342,804 - LRK54_RS01585 0.88 +0.7 342,804 - LRK54_RS01585 0.88 -2.1 342,804 - LRK54_RS01585 0.88 -0.1 342,804 - LRK54_RS01585 0.88 -3.7 342,804 - LRK54_RS01585 0.88 +0.7 342,804 - LRK54_RS01585 0.88 +0.7 342,804 - LRK54_RS01585 0.88 +0.7 342,822 - -2.2 342,891 + -1.1 342,891 + -0.9 342,891 + -1.6 342,891 + -0.4 342,891 + -1.2 342,891 + -0.3 342,891 + +0.3 342,891 + -0.5 342,891 + -0.3 342,891 + -2.6 342,891 + -0.3 342,891 + -0.9 342,892 - -0.3 342,892 - -0.0 342,892 - +0.7 342,892 - -0.3 342,892 - +0.7 342,892 - -0.4 342,892 - +0.2 342,892 - -0.7 342,892 - -0.3 342,892 - -0.3 342,892 - +0.1 343,003 + LRK54_RS01590 0.19 -2.2 343,003 + LRK54_RS01590 0.19 -1.2 343,003 + LRK54_RS01590 0.19 -2.1 343,003 + LRK54_RS01590 0.19 -0.4 343,003 + LRK54_RS01590 0.19 +0.7 343,004 - LRK54_RS01590 0.20 -0.5 343,045 + LRK54_RS01590 0.27 -0.3 343,045 + LRK54_RS01590 0.27 -0.5 343,045 + LRK54_RS01590 0.27 -1.6 343,045 + LRK54_RS01590 0.27 +0.7 343,045 + LRK54_RS01590 0.27 -1.3 343,045 + LRK54_RS01590 0.27 -2.6 343,045 + LRK54_RS01590 0.27 -1.3 343,045 + LRK54_RS01590 0.27 -1.0 343,045 + LRK54_RS01590 0.27 -1.2 343,045 + LRK54_RS01590 0.27 +0.3 343,045 + LRK54_RS01590 0.27 +0.7 343,045 + LRK54_RS01590 0.27 -2.5 343,045 + LRK54_RS01590 0.27 -2.6 343,045 + LRK54_RS01590 0.27 -2.3 343,046 - LRK54_RS01590 0.27 -0.9 343,046 - LRK54_RS01590 0.27 -1.1 343,046 - LRK54_RS01590 0.27 -1.1 343,046 - LRK54_RS01590 0.27 -0.3 343,046 - LRK54_RS01590 0.27 -0.5 343,046 - LRK54_RS01590 0.27 -2.0 343,046 - LRK54_RS01590 0.27 -1.3 343,046 - LRK54_RS01590 0.27 -2.6 343,046 - LRK54_RS01590 0.27 -3.1 343,046 - LRK54_RS01590 0.27 -1.8 343,046 - LRK54_RS01590 0.27 -1.8 343,046 - LRK54_RS01590 0.27 -1.9 343,046 - LRK54_RS01590 0.27 -1.5 343,046 - LRK54_RS01590 0.27 -1.8 343,046 - LRK54_RS01590 0.27 -2.8 343,046 - LRK54_RS01590 0.27 -1.4 343,046 - LRK54_RS01590 0.27 +0.3 343,046 - LRK54_RS01590 0.27 -1.9 343,046 - LRK54_RS01590 0.27 -1.1 343,046 - LRK54_RS01590 0.27 -0.3 343,046 - LRK54_RS01590 0.27 -0.9 343,046 - LRK54_RS01590 0.27 -1.6 343,046 - LRK54_RS01590 0.27 -1.9 343,060 + LRK54_RS01590 0.29 -2.0 343,060 + LRK54_RS01590 0.29 -1.8 343,060 + LRK54_RS01590 0.29 -1.0 343,060 + LRK54_RS01590 0.29 -1.3 343,060 + LRK54_RS01590 0.29 -1.6 343,061 - LRK54_RS01590 0.29 -1.9 343,061 - LRK54_RS01590 0.29 -1.2 343,061 - LRK54_RS01590 0.29 -1.6 343,061 - LRK54_RS01590 0.29 -2.2 343,061 - LRK54_RS01590 0.29 -1.1 343,391 + LRK54_RS01590 0.85 -0.3 343,426 + -1.8 343,426 + -0.3 343,426 + -1.3 343,426 + +0.7 343,426 + -2.3 343,427 - +0.7 343,427 - -0.6 343,431 + -0.9 343,431 + -0.9 343,431 + -1.0 343,443 - -0.3 343,482 + -1.3 343,482 + -1.4 343,482 + -1.9 343,482 + -1.3 343,482 + -0.6 343,482 + -1.4 343,482 + -0.3 343,482 + -1.4 343,483 - +1.3 343,483 - -0.4 343,483 - +0.3 343,483 - -1.8 343,483 - -1.0 343,483 - +0.2 343,483 - -0.3 343,483 - -1.4 343,483 - -0.0 343,483 - -2.3 343,483 - -0.4 343,540 + -2.0 343,540 + -2.3 343,540 + -0.1 343,540 + -0.8 343,541 - +0.5 343,541 - +0.1 343,589 + -1.9 343,589 + -2.6 343,589 + -0.7 343,589 + +0.8 343,589 + +0.6 343,589 + -0.3 343,589 + -0.1 343,589 + +0.7 343,589 + +0.9 343,589 + +0.2 343,589 + -1.3 343,589 + +1.1 343,589 + -0.2 343,589 + +0.3 343,589 + -0.7 343,589 + +0.7 343,589 + +3.0 343,589 + +1.3 343,589 + -1.6 343,589 + +0.4 343,589 + +0.1 343,589 + +1.5 343,589 + +0.5 343,589 + +0.4 343,590 - -0.1 343,590 - -0.9 343,590 - +1.6 343,590 - -0.1 343,590 - +1.0 343,590 - +0.0 343,590 - -1.4 343,590 - +0.1 343,590 - -2.1 343,590 - -0.7 343,590 - +0.2 343,590 - +1.4 343,590 - +0.2 343,590 - -1.0 343,590 - -0.7 343,590 - -0.1 343,590 - +1.1 343,590 - +1.7 343,590 - +0.7 343,590 - +2.0 343,606 - -1.1 343,823 + LRK54_RS01595 0.22 -0.5 343,823 + LRK54_RS01595 0.22 -0.9 343,823 + LRK54_RS01595 0.22 -0.1 343,823 + LRK54_RS01595 0.22 -0.3 343,823 + LRK54_RS01595 0.22 -0.9 343,823 + LRK54_RS01595 0.22 -3.1 343,824 - LRK54_RS01595 0.22 -0.5
Or see this region's nucleotide sequence