Strain Fitness in Bifidobacterium breve UCC2003 around BBR_RS12930

Experiment: Recovery broth with Ampicillin 4e-05 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBBR_RS12920 and BBR_RS12925 are separated by 181 nucleotidesBBR_RS12925 and BBR_RS12930 are separated by 273 nucleotidesBBR_RS12930 and BBR_RS12935 are separated by 20 nucleotides BBR_RS12920: BBR_RS12920 - glycine synthesis protein GlyXL (from data), at 650,899 to 652,263 _RS12920 BBR_RS12925: BBR_RS12925 - hypothetical protein, at 652,445 to 652,648 _RS12925 BBR_RS12930: BBR_RS12930 - tRNA (cytidine(34)-2'-O)-methyltransferase, at 652,922 to 653,584 _RS12930 BBR_RS12935: BBR_RS12935 - A/G-specific adenine glycosylase, at 653,605 to 654,567 _RS12935 Position (kb) 652 653 654Strain fitness (log2 ratio) -2 -1 0 1 2 3at 651.934 kb on - strand, within BBR_RS12920at 651.949 kb on + strand, within BBR_RS12920at 651.950 kb on + strand, within BBR_RS12920at 651.950 kb on + strand, within BBR_RS12920at 651.951 kb on + strand, within BBR_RS12920at 651.958 kb on + strand, within BBR_RS12920at 651.958 kb on + strand, within BBR_RS12920at 651.966 kb on + strand, within BBR_RS12920at 651.966 kb on + strand, within BBR_RS12920at 651.966 kb on + strand, within BBR_RS12920at 651.973 kb on + strand, within BBR_RS12920at 651.980 kb on - strand, within BBR_RS12920at 651.981 kb on - strand, within BBR_RS12920at 651.981 kb on - strand, within BBR_RS12920at 651.991 kb on - strand, within BBR_RS12920at 651.998 kb on + strand, within BBR_RS12920at 652.028 kb on + strand, within BBR_RS12920at 652.033 kb on + strand, within BBR_RS12920at 652.043 kb on + strand, within BBR_RS12920at 652.069 kb on + strand, within BBR_RS12920at 652.087 kb on - strand, within BBR_RS12920at 652.089 kb on + strand, within BBR_RS12920at 652.092 kb on + strand, within BBR_RS12920at 652.094 kb on + strand, within BBR_RS12920at 652.113 kb on - strand, within BBR_RS12920at 652.130 kb on - strandat 652.156 kb on + strandat 652.156 kb on + strandat 652.199 kb on + strandat 652.201 kb on + strandat 652.201 kb on + strandat 652.262 kb on + strandat 652.273 kb on + strandat 652.375 kb on + strandat 652.493 kb on + strand, within BBR_RS12925at 652.561 kb on - strand, within BBR_RS12925at 652.638 kb on + strandat 652.658 kb on - strandat 652.743 kb on + strandat 652.743 kb on - strandat 652.743 kb on - strandat 652.744 kb on - strandat 652.749 kb on - strandat 652.830 kb on - strandat 652.845 kb on - strandat 652.849 kb on - strandat 652.849 kb on - strandat 652.913 kb on + strandat 652.949 kb on + strandat 652.953 kb on - strandat 652.957 kb on - strandat 652.990 kb on + strand, within BBR_RS12930at 652.990 kb on + strand, within BBR_RS12930at 652.990 kb on + strand, within BBR_RS12930at 652.990 kb on + strand, within BBR_RS12930at 652.990 kb on + strand, within BBR_RS12930at 652.990 kb on + strand, within BBR_RS12930at 652.990 kb on + strand, within BBR_RS12930at 652.990 kb on + strand, within BBR_RS12930at 652.998 kb on - strand, within BBR_RS12930at 652.998 kb on - strand, within BBR_RS12930at 652.998 kb on - strand, within BBR_RS12930at 652.998 kb on - strand, within BBR_RS12930at 652.998 kb on - strand, within BBR_RS12930at 652.998 kb on - strand, within BBR_RS12930at 652.998 kb on - strand, within BBR_RS12930at 652.998 kb on - strand, within BBR_RS12930at 652.998 kb on - strand, within BBR_RS12930at 652.998 kb on - strand, within BBR_RS12930at 652.998 kb on - strand, within BBR_RS12930at 652.998 kb on - strand, within BBR_RS12930at 652.998 kb on - strand, within BBR_RS12930at 653.011 kb on + strand, within BBR_RS12930at 653.013 kb on + strand, within BBR_RS12930at 653.032 kb on + strand, within BBR_RS12930at 653.068 kb on + strand, within BBR_RS12930at 653.086 kb on + strand, within BBR_RS12930at 653.096 kb on + strand, within BBR_RS12930at 653.096 kb on + strand, within BBR_RS12930at 653.135 kb on + strand, within BBR_RS12930at 653.157 kb on - strand, within BBR_RS12930at 653.187 kb on + strand, within BBR_RS12930at 653.199 kb on - strand, within BBR_RS12930at 653.204 kb on - strand, within BBR_RS12930at 653.204 kb on - strand, within BBR_RS12930at 653.223 kb on + strand, within BBR_RS12930at 653.225 kb on - strand, within BBR_RS12930at 653.235 kb on - strand, within BBR_RS12930at 653.235 kb on - strand, within BBR_RS12930at 653.242 kb on + strand, within BBR_RS12930at 653.243 kb on + strand, within BBR_RS12930at 653.251 kb on - strand, within BBR_RS12930at 653.263 kb on + strand, within BBR_RS12930at 653.271 kb on - strand, within BBR_RS12930at 653.276 kb on - strand, within BBR_RS12930at 653.278 kb on + strand, within BBR_RS12930at 653.286 kb on - strand, within BBR_RS12930at 653.286 kb on - strand, within BBR_RS12930at 653.286 kb on - strand, within BBR_RS12930at 653.286 kb on - strand, within BBR_RS12930at 653.286 kb on - strand, within BBR_RS12930at 653.286 kb on - strand, within BBR_RS12930at 653.368 kb on + strand, within BBR_RS12930at 653.370 kb on + strand, within BBR_RS12930at 653.371 kb on - strand, within BBR_RS12930at 653.378 kb on + strand, within BBR_RS12930at 653.378 kb on - strand, within BBR_RS12930at 653.389 kb on + strand, within BBR_RS12930at 653.399 kb on - strand, within BBR_RS12930at 653.421 kb on + strand, within BBR_RS12930at 653.429 kb on + strand, within BBR_RS12930at 653.451 kb on + strand, within BBR_RS12930at 653.459 kb on - strand, within BBR_RS12930at 653.483 kb on - strand, within BBR_RS12930at 653.602 kb on - strandat 653.605 kb on + strandat 653.639 kb on + strandat 653.639 kb on + strandat 653.639 kb on - strandat 653.643 kb on + strandat 653.651 kb on - strandat 653.685 kb on + strandat 653.685 kb on + strandat 653.692 kb on - strandat 653.711 kb on + strand, within BBR_RS12935at 653.711 kb on + strand, within BBR_RS12935at 653.715 kb on + strand, within BBR_RS12935at 653.736 kb on + strand, within BBR_RS12935at 653.736 kb on + strand, within BBR_RS12935at 653.780 kb on + strand, within BBR_RS12935at 653.780 kb on + strand, within BBR_RS12935at 653.787 kb on + strand, within BBR_RS12935at 653.788 kb on - strand, within BBR_RS12935at 653.788 kb on - strand, within BBR_RS12935at 653.788 kb on - strand, within BBR_RS12935at 653.797 kb on - strand, within BBR_RS12935at 653.797 kb on - strand, within BBR_RS12935at 653.809 kb on + strand, within BBR_RS12935at 653.809 kb on + strand, within BBR_RS12935at 653.809 kb on + strand, within BBR_RS12935at 653.816 kb on - strand, within BBR_RS12935at 653.816 kb on - strand, within BBR_RS12935at 653.816 kb on - strand, within BBR_RS12935at 653.818 kb on + strand, within BBR_RS12935at 653.826 kb on - strand, within BBR_RS12935at 653.826 kb on - strand, within BBR_RS12935at 653.916 kb on + strand, within BBR_RS12935at 653.916 kb on + strand, within BBR_RS12935at 653.916 kb on + strand, within BBR_RS12935at 653.930 kb on - strand, within BBR_RS12935at 653.946 kb on + strand, within BBR_RS12935at 653.959 kb on - strand, within BBR_RS12935at 653.963 kb on + strand, within BBR_RS12935at 653.966 kb on - strand, within BBR_RS12935at 653.977 kb on + strand, within BBR_RS12935at 653.977 kb on + strand, within BBR_RS12935at 653.977 kb on + strand, within BBR_RS12935at 653.977 kb on + strand, within BBR_RS12935at 653.977 kb on + strand, within BBR_RS12935at 653.977 kb on + strand, within BBR_RS12935at 653.985 kb on - strand, within BBR_RS12935at 653.985 kb on - strand, within BBR_RS12935at 654.052 kb on - strand, within BBR_RS12935at 654.052 kb on - strand, within BBR_RS12935at 654.084 kb on - strand, within BBR_RS12935at 654.110 kb on - strand, within BBR_RS12935at 654.123 kb on + strand, within BBR_RS12935at 654.124 kb on + strand, within BBR_RS12935at 654.124 kb on - strand, within BBR_RS12935at 654.147 kb on + strand, within BBR_RS12935at 654.147 kb on + strand, within BBR_RS12935at 654.161 kb on + strand, within BBR_RS12935at 654.190 kb on + strand, within BBR_RS12935at 654.195 kb on + strand, within BBR_RS12935at 654.195 kb on - strand, within BBR_RS12935at 654.226 kb on - strand, within BBR_RS12935at 654.240 kb on - strand, within BBR_RS12935at 654.245 kb on + strand, within BBR_RS12935at 654.260 kb on - strand, within BBR_RS12935at 654.260 kb on - strand, within BBR_RS12935at 654.262 kb on + strand, within BBR_RS12935at 654.270 kb on - strand, within BBR_RS12935at 654.312 kb on + strand, within BBR_RS12935at 654.332 kb on + strand, within BBR_RS12935at 654.332 kb on + strand, within BBR_RS12935at 654.369 kb on + strand, within BBR_RS12935at 654.377 kb on + strand, within BBR_RS12935at 654.382 kb on + strand, within BBR_RS12935at 654.387 kb on + strand, within BBR_RS12935at 654.417 kb on - strand, within BBR_RS12935at 654.480 kb on - strandat 654.535 kb on - strandat 654.539 kb on + strandat 654.555 kb on + strandat 654.577 kb on + strandat 654.577 kb on + strandat 654.577 kb on + strandat 654.577 kb on + strandat 654.577 kb on + strandat 654.577 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Recovery broth with Ampicillin 4e-05 mM
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651,934 - BBR_RS12920 0.76 +0.3
651,949 + BBR_RS12920 0.77 -0.4
651,950 + BBR_RS12920 0.77 +0.5
651,950 + BBR_RS12920 0.77 -0.0
651,951 + BBR_RS12920 0.77 +0.1
651,958 + BBR_RS12920 0.78 -0.1
651,958 + BBR_RS12920 0.78 -0.5
651,966 + BBR_RS12920 0.78 -0.0
651,966 + BBR_RS12920 0.78 +0.9
651,966 + BBR_RS12920 0.78 -0.5
651,973 + BBR_RS12920 0.79 +0.4
651,980 - BBR_RS12920 0.79 -0.3
651,981 - BBR_RS12920 0.79 +1.3
651,981 - BBR_RS12920 0.79 -0.2
651,991 - BBR_RS12920 0.80 -0.6
651,998 + BBR_RS12920 0.81 -0.4
652,028 + BBR_RS12920 0.83 +0.4
652,033 + BBR_RS12920 0.83 +0.8
652,043 + BBR_RS12920 0.84 +0.4
652,069 + BBR_RS12920 0.86 -0.3
652,087 - BBR_RS12920 0.87 -0.6
652,089 + BBR_RS12920 0.87 +0.2
652,092 + BBR_RS12920 0.87 +2.5
652,094 + BBR_RS12920 0.88 -0.7
652,113 - BBR_RS12920 0.89 -0.5
652,130 - -0.5
652,156 + -0.2
652,156 + +0.3
652,199 + +0.0
652,201 + +0.6
652,201 + +1.0
652,262 + -0.2
652,273 + +0.7
652,375 + +0.1
652,493 + BBR_RS12925 0.24 -0.6
652,561 - BBR_RS12925 0.57 +0.4
652,638 + +1.1
652,658 - -0.1
652,743 + -0.5
652,743 - -0.2
652,743 - +0.5
652,744 - -2.0
652,749 - -0.2
652,830 - +0.7
652,845 - +0.2
652,849 - +1.0
652,849 - -0.3
652,913 + +1.1
652,949 + -0.0
652,953 - -2.0
652,957 - -0.0
652,990 + BBR_RS12930 0.10 -0.5
652,990 + BBR_RS12930 0.10 +0.1
652,990 + BBR_RS12930 0.10 -0.3
652,990 + BBR_RS12930 0.10 +0.9
652,990 + BBR_RS12930 0.10 +1.3
652,990 + BBR_RS12930 0.10 -1.2
652,990 + BBR_RS12930 0.10 -0.3
652,990 + BBR_RS12930 0.10 +0.3
652,998 - BBR_RS12930 0.11 +0.7
652,998 - BBR_RS12930 0.11 +0.6
652,998 - BBR_RS12930 0.11 -1.6
652,998 - BBR_RS12930 0.11 -0.9
652,998 - BBR_RS12930 0.11 -0.2
652,998 - BBR_RS12930 0.11 +1.0
652,998 - BBR_RS12930 0.11 +0.3
652,998 - BBR_RS12930 0.11 -0.4
652,998 - BBR_RS12930 0.11 +0.8
652,998 - BBR_RS12930 0.11 -0.8
652,998 - BBR_RS12930 0.11 +1.0
652,998 - BBR_RS12930 0.11 +1.9
652,998 - BBR_RS12930 0.11 +0.2
653,011 + BBR_RS12930 0.13 -0.1
653,013 + BBR_RS12930 0.14 +0.6
653,032 + BBR_RS12930 0.17 -1.1
653,068 + BBR_RS12930 0.22 -0.7
653,086 + BBR_RS12930 0.25 -0.9
653,096 + BBR_RS12930 0.26 -0.7
653,096 + BBR_RS12930 0.26 +0.2
653,135 + BBR_RS12930 0.32 +0.1
653,157 - BBR_RS12930 0.35 +0.6
653,187 + BBR_RS12930 0.40 +0.8
653,199 - BBR_RS12930 0.42 -1.2
653,204 - BBR_RS12930 0.43 -0.4
653,204 - BBR_RS12930 0.43 -0.4
653,223 + BBR_RS12930 0.45 -0.8
653,225 - BBR_RS12930 0.46 -1.5
653,235 - BBR_RS12930 0.47 -0.4
653,235 - BBR_RS12930 0.47 +0.7
653,242 + BBR_RS12930 0.48 +0.8
653,243 + BBR_RS12930 0.48 +0.6
653,251 - BBR_RS12930 0.50 -0.3
653,263 + BBR_RS12930 0.51 +3.0
653,271 - BBR_RS12930 0.53 +0.0
653,276 - BBR_RS12930 0.53 -0.3
653,278 + BBR_RS12930 0.54 -0.2
653,286 - BBR_RS12930 0.55 -1.0
653,286 - BBR_RS12930 0.55 -0.8
653,286 - BBR_RS12930 0.55 +0.1
653,286 - BBR_RS12930 0.55 -1.6
653,286 - BBR_RS12930 0.55 -1.5
653,286 - BBR_RS12930 0.55 -0.7
653,368 + BBR_RS12930 0.67 +0.2
653,370 + BBR_RS12930 0.68 -0.6
653,371 - BBR_RS12930 0.68 +1.0
653,378 + BBR_RS12930 0.69 -0.5
653,378 - BBR_RS12930 0.69 +1.0
653,389 + BBR_RS12930 0.70 -1.4
653,399 - BBR_RS12930 0.72 +0.9
653,421 + BBR_RS12930 0.75 +1.3
653,429 + BBR_RS12930 0.76 -0.1
653,451 + BBR_RS12930 0.80 -0.5
653,459 - BBR_RS12930 0.81 +0.4
653,483 - BBR_RS12930 0.85 +0.2
653,602 - -0.7
653,605 + +1.2
653,639 + -0.4
653,639 + +0.1
653,639 - -0.3
653,643 + +0.9
653,651 - +0.8
653,685 + +0.3
653,685 + +0.0
653,692 - -1.2
653,711 + BBR_RS12935 0.11 -0.5
653,711 + BBR_RS12935 0.11 +0.5
653,715 + BBR_RS12935 0.11 -0.6
653,736 + BBR_RS12935 0.14 -0.4
653,736 + BBR_RS12935 0.14 -0.0
653,780 + BBR_RS12935 0.18 +0.4
653,780 + BBR_RS12935 0.18 +0.9
653,787 + BBR_RS12935 0.19 +0.2
653,788 - BBR_RS12935 0.19 +0.7
653,788 - BBR_RS12935 0.19 +0.1
653,788 - BBR_RS12935 0.19 +0.4
653,797 - BBR_RS12935 0.20 -0.5
653,797 - BBR_RS12935 0.20 +0.2
653,809 + BBR_RS12935 0.21 +0.9
653,809 + BBR_RS12935 0.21 +0.3
653,809 + BBR_RS12935 0.21 +0.1
653,816 - BBR_RS12935 0.22 +0.1
653,816 - BBR_RS12935 0.22 -0.2
653,816 - BBR_RS12935 0.22 -0.7
653,818 + BBR_RS12935 0.22 +0.2
653,826 - BBR_RS12935 0.23 -1.8
653,826 - BBR_RS12935 0.23 -0.0
653,916 + BBR_RS12935 0.32 +0.3
653,916 + BBR_RS12935 0.32 -0.2
653,916 + BBR_RS12935 0.32 -1.1
653,930 - BBR_RS12935 0.34 -0.6
653,946 + BBR_RS12935 0.35 -0.6
653,959 - BBR_RS12935 0.37 -0.2
653,963 + BBR_RS12935 0.37 +0.6
653,966 - BBR_RS12935 0.37 +1.6
653,977 + BBR_RS12935 0.39 +1.1
653,977 + BBR_RS12935 0.39 -1.9
653,977 + BBR_RS12935 0.39 -1.6
653,977 + BBR_RS12935 0.39 -0.3
653,977 + BBR_RS12935 0.39 +0.0
653,977 + BBR_RS12935 0.39 +0.2
653,985 - BBR_RS12935 0.39 -0.8
653,985 - BBR_RS12935 0.39 -0.4
654,052 - BBR_RS12935 0.46 +0.3
654,052 - BBR_RS12935 0.46 -0.6
654,084 - BBR_RS12935 0.50 +1.3
654,110 - BBR_RS12935 0.52 -2.5
654,123 + BBR_RS12935 0.54 -0.1
654,124 + BBR_RS12935 0.54 -1.6
654,124 - BBR_RS12935 0.54 +0.6
654,147 + BBR_RS12935 0.56 -0.2
654,147 + BBR_RS12935 0.56 +0.4
654,161 + BBR_RS12935 0.58 -0.4
654,190 + BBR_RS12935 0.61 -0.3
654,195 + BBR_RS12935 0.61 -2.5
654,195 - BBR_RS12935 0.61 +0.1
654,226 - BBR_RS12935 0.64 -1.2
654,240 - BBR_RS12935 0.66 -0.1
654,245 + BBR_RS12935 0.66 +1.4
654,260 - BBR_RS12935 0.68 +1.4
654,260 - BBR_RS12935 0.68 -0.4
654,262 + BBR_RS12935 0.68 +0.6
654,270 - BBR_RS12935 0.69 +0.3
654,312 + BBR_RS12935 0.73 -0.9
654,332 + BBR_RS12935 0.75 +2.0
654,332 + BBR_RS12935 0.75 +1.0
654,369 + BBR_RS12935 0.79 +0.3
654,377 + BBR_RS12935 0.80 -0.2
654,382 + BBR_RS12935 0.81 -0.9
654,387 + BBR_RS12935 0.81 +0.2
654,417 - BBR_RS12935 0.84 +0.0
654,480 - -0.4
654,535 - -0.8
654,539 + -1.5
654,555 + -0.8
654,577 + -0.3
654,577 + -0.4
654,577 + -0.7
654,577 + -1.2
654,577 + -0.1
654,577 + +0.2

Or see this region's nucleotide sequence