Experiment: L-Leucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS01665 and AZOBR_RS01670 are separated by 52 nucleotides AZOBR_RS01670 and AZOBR_RS01675 are separated by 2 nucleotides AZOBR_RS01675 and AZOBR_RS01680 are separated by 14 nucleotides AZOBR_RS01680 and AZOBR_RS01685 overlap by 1 nucleotides
AZOBR_RS01665: AZOBR_RS01665 - imidazoleglycerol-phosphate dehydratase, at 342,356 to 342,979
_RS01665
AZOBR_RS01670: AZOBR_RS01670 - imidazole glycerol phosphate synthase, at 343,032 to 343,676
_RS01670
AZOBR_RS01675: AZOBR_RS01675 - acetyltransferase, at 343,679 to 344,221
_RS01675
AZOBR_RS01680: AZOBR_RS01680 - 1-(5-phosphoribosyl)-5-((5-phosphoribosylamino)methylideneamino)imidazole-4-carboxamide isomerase (EC 5.3.1.16) <small>(from data)</small>, at 344,236 to 344,985
_RS01680
AZOBR_RS01685: AZOBR_RS01685 - imidazole glycerol phosphate synthase, at 344,985 to 345,782
_RS01685
Position (kb) 343
344
345 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 342.719 kb on + strand, within AZOBR_RS01665 at 342.719 kb on + strand, within AZOBR_RS01665 at 342.720 kb on - strand, within AZOBR_RS01665 at 342.878 kb on + strand, within AZOBR_RS01665 at 342.878 kb on + strand, within AZOBR_RS01665 at 342.879 kb on - strand, within AZOBR_RS01665 at 343.491 kb on + strand, within AZOBR_RS01670 at 343.492 kb on - strand, within AZOBR_RS01670 at 343.821 kb on - strand, within AZOBR_RS01675 at 344.096 kb on + strand, within AZOBR_RS01675 at 344.138 kb on + strand, within AZOBR_RS01675 at 344.138 kb on + strand, within AZOBR_RS01675 at 344.177 kb on + strand at 344.177 kb on + strand at 344.178 kb on - strand at 344.178 kb on - strand at 344.178 kb on - strand at 344.219 kb on + strand at 344.220 kb on - strand at 344.220 kb on - strand at 344.245 kb on + strand at 344.245 kb on + strand at 344.246 kb on - strand at 344.635 kb on + strand, within AZOBR_RS01680 at 344.635 kb on + strand, within AZOBR_RS01680 at 344.635 kb on + strand, within AZOBR_RS01680 at 344.636 kb on - strand, within AZOBR_RS01680 at 344.636 kb on - strand, within AZOBR_RS01680 at 344.636 kb on - strand, within AZOBR_RS01680 at 344.729 kb on - strand, within AZOBR_RS01680 at 344.729 kb on - strand, within AZOBR_RS01680 at 345.171 kb on + strand, within AZOBR_RS01685 at 345.171 kb on + strand, within AZOBR_RS01685 at 345.171 kb on + strand, within AZOBR_RS01685 at 345.172 kb on - strand, within AZOBR_RS01685 at 345.172 kb on - strand, within AZOBR_RS01685 at 345.172 kb on - strand, within AZOBR_RS01685 at 345.172 kb on - strand, within AZOBR_RS01685
Per-strain Table
Position Strand Gene LocusTag Fraction L-Leucine (N) remove 342,719 + AZOBR_RS01665 0.58 -2.9 342,719 + AZOBR_RS01665 0.58 -3.3 342,720 - AZOBR_RS01665 0.58 -5.6 342,878 + AZOBR_RS01665 0.84 +0.5 342,878 + AZOBR_RS01665 0.84 +0.5 342,879 - AZOBR_RS01665 0.84 -2.9 343,491 + AZOBR_RS01670 0.71 -4.6 343,492 - AZOBR_RS01670 0.71 -4.3 343,821 - AZOBR_RS01675 0.26 -3.6 344,096 + AZOBR_RS01675 0.77 +0.7 344,138 + AZOBR_RS01675 0.85 -2.6 344,138 + AZOBR_RS01675 0.85 -0.2 344,177 + +0.6 344,177 + -0.1 344,178 - -2.8 344,178 - -1.4 344,178 - -4.2 344,219 + +0.5 344,220 - -2.5 344,220 - -0.5 344,245 + -0.8 344,245 + -2.4 344,246 - -1.6 344,635 + AZOBR_RS01680 0.53 -2.7 344,635 + AZOBR_RS01680 0.53 -3.6 344,635 + AZOBR_RS01680 0.53 -2.5 344,636 - AZOBR_RS01680 0.53 -0.3 344,636 - AZOBR_RS01680 0.53 -2.6 344,636 - AZOBR_RS01680 0.53 -1.5 344,729 - AZOBR_RS01680 0.66 -4.7 344,729 - AZOBR_RS01680 0.66 -4.7 345,171 + AZOBR_RS01685 0.23 -1.8 345,171 + AZOBR_RS01685 0.23 -1.2 345,171 + AZOBR_RS01685 0.23 -1.0 345,172 - AZOBR_RS01685 0.23 -3.2 345,172 - AZOBR_RS01685 0.23 -3.1 345,172 - AZOBR_RS01685 0.23 -3.5 345,172 - AZOBR_RS01685 0.23 -3.7
Or see this region's nucleotide sequence