Experiment: MoYLS4 with A22 0.043 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_2283 and flgB are separated by 659 nucleotides flgB and DvMF_2285 overlap by 1 nucleotides DvMF_2285 and DvMF_2286 are separated by 19 nucleotides
DvMF_2283: DvMF_2283 - TPR repeat-containing protein (RefSeq), at 2,871,815 to 2,872,375
_2283
DvMF_2284: flgB - flagellar basal body rod protein FlgB (RefSeq), at 2,873,035 to 2,873,445
flgB
DvMF_2285: DvMF_2285 - flagellar basal-body rod protein FlgC (RefSeq), at 2,873,445 to 2,873,882
_2285
DvMF_2286: DvMF_2286 - flagellar hook-basal body complex subunit FliE (RefSeq), at 2,873,902 to 2,874,234
_2286
Position (kb)
2873
2874 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2872.200 kb on + strand, within DvMF_2283 at 2872.406 kb on + strand at 2872.495 kb on - strand at 2872.495 kb on - strand at 2872.559 kb on + strand at 2872.695 kb on + strand at 2872.709 kb on + strand at 2873.064 kb on - strand at 2873.236 kb on + strand, within flgB at 2873.403 kb on + strand, within flgB at 2873.566 kb on - strand, within DvMF_2285 at 2873.566 kb on - strand, within DvMF_2285 at 2873.817 kb on - strand, within DvMF_2285 at 2874.020 kb on - strand, within DvMF_2286 at 2874.219 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with A22 0.043 mg/ml remove 2,872,200 + DvMF_2283 0.69 +1.9 2,872,406 + -0.3 2,872,495 - -0.2 2,872,495 - -2.6 2,872,559 + -0.1 2,872,695 + -0.3 2,872,709 + +0.5 2,873,064 - -1.0 2,873,236 + flgB DvMF_2284 0.49 -1.2 2,873,403 + flgB DvMF_2284 0.90 -1.2 2,873,566 - DvMF_2285 0.28 -1.2 2,873,566 - DvMF_2285 0.28 -1.3 2,873,817 - DvMF_2285 0.85 -1.9 2,874,020 - DvMF_2286 0.35 -1.4 2,874,219 - -1.2
Or see this region's nucleotide sequence