Experiment: MoYLS4 with A22 0.043 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_1000 and DvMF_1001 are separated by 96 nucleotides DvMF_1001 and DvMF_1002 overlap by 4 nucleotides
DvMF_1000: DvMF_1000 - biotin/acetyl-CoA-carboxylase ligase (RefSeq), at 1,215,217 to 1,216,215
_1000
DvMF_1001: DvMF_1001 - biotin synthase (RefSeq), at 1,216,312 to 1,217,406
_1001
DvMF_1002: DvMF_1002 - adenosylmethionine-8-amino-7-oxononanoate aminotransferase (RefSeq), at 1,217,403 to 1,218,878
_1002
Position (kb)
1216
1217
1218 Strain fitness (log2 ratio)
-2
-1
0
1 at 1215.589 kb on + strand, within DvMF_1000 at 1215.897 kb on - strand, within DvMF_1000 at 1215.957 kb on + strand, within DvMF_1000 at 1215.965 kb on - strand, within DvMF_1000 at 1215.965 kb on - strand, within DvMF_1000 at 1216.096 kb on - strand, within DvMF_1000 at 1216.935 kb on + strand, within DvMF_1001 at 1216.943 kb on - strand, within DvMF_1001 at 1217.149 kb on + strand, within DvMF_1001 at 1217.288 kb on + strand, within DvMF_1001 at 1217.337 kb on + strand at 1217.435 kb on - strand at 1217.492 kb on + strand at 1217.492 kb on + strand at 1217.492 kb on + strand at 1217.500 kb on - strand at 1217.513 kb on + strand at 1217.636 kb on - strand, within DvMF_1002 at 1217.726 kb on - strand, within DvMF_1002 at 1217.778 kb on - strand, within DvMF_1002 at 1217.952 kb on - strand, within DvMF_1002 at 1217.972 kb on - strand, within DvMF_1002 at 1217.996 kb on + strand, within DvMF_1002 at 1218.112 kb on + strand, within DvMF_1002 at 1218.319 kb on + strand, within DvMF_1002
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with A22 0.043 mg/ml remove 1,215,589 + DvMF_1000 0.37 -0.5 1,215,897 - DvMF_1000 0.68 -0.7 1,215,957 + DvMF_1000 0.74 +0.9 1,215,965 - DvMF_1000 0.75 -0.3 1,215,965 - DvMF_1000 0.75 +0.4 1,216,096 - DvMF_1000 0.88 +0.0 1,216,935 + DvMF_1001 0.57 -0.2 1,216,943 - DvMF_1001 0.58 -0.1 1,217,149 + DvMF_1001 0.76 -0.1 1,217,288 + DvMF_1001 0.89 -0.2 1,217,337 + -0.1 1,217,435 - -0.4 1,217,492 + -0.4 1,217,492 + -2.0 1,217,492 + -0.2 1,217,500 - -0.3 1,217,513 + -0.2 1,217,636 - DvMF_1002 0.16 -0.3 1,217,726 - DvMF_1002 0.22 -0.0 1,217,778 - DvMF_1002 0.25 +0.2 1,217,952 - DvMF_1002 0.37 -0.1 1,217,972 - DvMF_1002 0.39 +0.0 1,217,996 + DvMF_1002 0.40 -0.6 1,218,112 + DvMF_1002 0.48 -0.3 1,218,319 + DvMF_1002 0.62 -0.3
Or see this region's nucleotide sequence