Experiment: MoYLS4 with A22 0.043 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_0696 and DvMF_0697 overlap by 4 nucleotides DvMF_0697 and DvMF_0698 overlap by 1 nucleotides DvMF_0698 and DvMF_0699 are separated by 44 nucleotides
DvMF_0696: DvMF_0696 - secretion protein HlyD family protein (RefSeq), at 843,362 to 844,423
_0696
DvMF_0697: DvMF_0697 - ABC transporter related (RefSeq), at 844,420 to 845,118
_0697
DvMF_0698: DvMF_0698 - protein of unknown function DUF214 (RefSeq), at 845,118 to 846,248
_0698
DvMF_0699: DvMF_0699 - putative two component, sigma54 specific, transcriptional regulator, Fis family (RefSeq), at 846,293 to 847,762
_0699
Position (kb)
845
846
847 Strain fitness (log2 ratio)
-1
0
1 at 844.237 kb on - strand, within DvMF_0696 at 844.248 kb on - strand, within DvMF_0696 at 844.254 kb on + strand, within DvMF_0696 at 844.262 kb on - strand, within DvMF_0696 at 844.327 kb on + strand at 844.660 kb on - strand, within DvMF_0697 at 845.463 kb on + strand, within DvMF_0698 at 845.631 kb on - strand, within DvMF_0698 at 845.844 kb on + strand, within DvMF_0698 at 845.983 kb on - strand, within DvMF_0698 at 845.995 kb on + strand, within DvMF_0698 at 846.680 kb on - strand, within DvMF_0699 at 846.747 kb on + strand, within DvMF_0699 at 846.747 kb on + strand, within DvMF_0699 at 846.830 kb on - strand, within DvMF_0699
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with A22 0.043 mg/ml remove 844,237 - DvMF_0696 0.82 -0.7 844,248 - DvMF_0696 0.83 +0.1 844,254 + DvMF_0696 0.84 -0.2 844,262 - DvMF_0696 0.85 -0.7 844,327 + -0.5 844,660 - DvMF_0697 0.34 -0.5 845,463 + DvMF_0698 0.31 -0.2 845,631 - DvMF_0698 0.45 -0.0 845,844 + DvMF_0698 0.64 +0.5 845,983 - DvMF_0698 0.76 -0.1 845,995 + DvMF_0698 0.78 +0.5 846,680 - DvMF_0699 0.26 +0.1 846,747 + DvMF_0699 0.31 +0.1 846,747 + DvMF_0699 0.31 -1.4 846,830 - DvMF_0699 0.37 -0.2
Or see this region's nucleotide sequence