Experiment: MoYLS4 with A22 0.043 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_0496 and DvMF_0497 are separated by 8 nucleotides DvMF_0497 and rpsU are separated by 131 nucleotides rpsU and DvMF_0499 are separated by 195 nucleotides DvMF_0499 and DvMF_0500 are separated by 15 nucleotides DvMF_0500 and ksgA are separated by 191 nucleotides
DvMF_0496: DvMF_0496 - Smr protein/MutS2 (RefSeq), at 583,728 to 586,181
_0496
DvMF_0497: DvMF_0497 - GatB/YqeY family protein (RefSeq), at 586,190 to 586,636
_0497
DvMF_0498: rpsU - 30S ribosomal protein S21 (RefSeq), at 586,768 to 586,971
rpsU
DvMF_0499: DvMF_0499 - hypothetical protein (RefSeq), at 587,167 to 587,355
_0499
DvMF_0500: DvMF_0500 - histone family protein DNA-binding protein (RefSeq), at 587,371 to 587,643
_0500
DvMF_0501: ksgA - dimethyladenosine transferase (RefSeq), at 587,835 to 588,647
ksgA
Position (kb) 586
587 Strain fitness (log2 ratio) -2
-1
0
1 at 585.898 kb on - strand, within DvMF_0496 at 585.911 kb on - strand, within DvMF_0496 at 586.027 kb on + strand at 586.035 kb on - strand at 586.090 kb on - strand at 586.160 kb on + strand at 586.278 kb on - strand, within DvMF_0497 at 586.364 kb on + strand, within DvMF_0497 at 587.057 kb on - strand at 587.178 kb on - strand at 587.259 kb on + strand, within DvMF_0499 at 587.482 kb on + strand, within DvMF_0500 at 587.514 kb on + strand, within DvMF_0500 at 587.522 kb on - strand, within DvMF_0500 at 587.590 kb on - strand, within DvMF_0500 at 587.633 kb on + strand at 587.792 kb on - strand at 587.792 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with A22 0.043 mg/ml remove 585,898 - DvMF_0496 0.88 -0.3 585,911 - DvMF_0496 0.89 -0.3 586,027 + -0.1 586,035 - -0.4 586,090 - -0.4 586,160 + +0.3 586,278 - DvMF_0497 0.20 -2.0 586,364 + DvMF_0497 0.39 -1.8 587,057 - -0.4 587,178 - -0.0 587,259 + DvMF_0499 0.49 -0.2 587,482 + DvMF_0500 0.41 -0.7 587,514 + DvMF_0500 0.52 -0.2 587,522 - DvMF_0500 0.55 +0.1 587,590 - DvMF_0500 0.80 -0.2 587,633 + +0.3 587,792 - +0.1 587,792 - -0.0
Or see this region's nucleotide sequence