Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_519 and Pf6N2E2_520 are separated by 10 nucleotides Pf6N2E2_520 and Pf6N2E2_521 are separated by 15 nucleotides Pf6N2E2_521 and Pf6N2E2_522 are separated by 51 nucleotides
Pf6N2E2_519 - Myo-inositol 2-dehydrogenase (EC 1.1.1.18), at 589,541 to 590,593
_519
Pf6N2E2_520 - hypothetical protein, at 590,604 to 590,720
_520
Pf6N2E2_521 - hypothetical protein, at 590,736 to 590,888
_521
Pf6N2E2_522 - Inositol transport system sugar-binding protein, at 590,940 to 591,866
_522
Position (kb) 590
591 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 589.998 kb on - strand, within Pf6N2E2_519 at 590.121 kb on - strand, within Pf6N2E2_519 at 590.261 kb on + strand, within Pf6N2E2_519 at 590.262 kb on - strand, within Pf6N2E2_519 at 590.262 kb on - strand, within Pf6N2E2_519 at 590.262 kb on - strand, within Pf6N2E2_519 at 590.429 kb on + strand, within Pf6N2E2_519 at 590.430 kb on - strand, within Pf6N2E2_519 at 590.430 kb on - strand, within Pf6N2E2_519 at 590.430 kb on - strand, within Pf6N2E2_519 at 590.430 kb on - strand, within Pf6N2E2_519 at 590.430 kb on - strand, within Pf6N2E2_519 at 590.430 kb on - strand, within Pf6N2E2_519 at 590.724 kb on + strand at 590.724 kb on + strand at 590.724 kb on + strand at 590.724 kb on + strand at 590.725 kb on - strand at 590.729 kb on - strand at 590.763 kb on + strand, within Pf6N2E2_521 at 590.763 kb on + strand, within Pf6N2E2_521 at 590.763 kb on + strand, within Pf6N2E2_521 at 590.763 kb on + strand, within Pf6N2E2_521 at 590.764 kb on - strand, within Pf6N2E2_521 at 590.764 kb on - strand, within Pf6N2E2_521 at 590.907 kb on + strand at 591.060 kb on + strand, within Pf6N2E2_522 at 591.061 kb on - strand, within Pf6N2E2_522 at 591.061 kb on - strand, within Pf6N2E2_522 at 591.478 kb on - strand, within Pf6N2E2_522 at 591.658 kb on + strand, within Pf6N2E2_522 at 591.770 kb on + strand, within Pf6N2E2_522 at 591.770 kb on + strand, within Pf6N2E2_522
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 589,998 - Pf6N2E2_519 0.43 +1.1 590,121 - Pf6N2E2_519 0.55 +0.1 590,261 + Pf6N2E2_519 0.68 +0.8 590,262 - Pf6N2E2_519 0.68 +0.3 590,262 - Pf6N2E2_519 0.68 +0.3 590,262 - Pf6N2E2_519 0.68 -0.2 590,429 + Pf6N2E2_519 0.84 +1.0 590,430 - Pf6N2E2_519 0.84 +0.0 590,430 - Pf6N2E2_519 0.84 +0.8 590,430 - Pf6N2E2_519 0.84 +0.1 590,430 - Pf6N2E2_519 0.84 +0.2 590,430 - Pf6N2E2_519 0.84 +0.6 590,430 - Pf6N2E2_519 0.84 +0.4 590,724 + +0.9 590,724 + +0.6 590,724 + +0.3 590,724 + +0.8 590,725 - -0.5 590,729 - -0.7 590,763 + Pf6N2E2_521 0.18 -1.7 590,763 + Pf6N2E2_521 0.18 +0.5 590,763 + Pf6N2E2_521 0.18 -0.3 590,763 + Pf6N2E2_521 0.18 -2.8 590,764 - Pf6N2E2_521 0.18 +0.3 590,764 - Pf6N2E2_521 0.18 +0.1 590,907 + -0.1 591,060 + Pf6N2E2_522 0.13 +0.2 591,061 - Pf6N2E2_522 0.13 +0.0 591,061 - Pf6N2E2_522 0.13 -1.9 591,478 - Pf6N2E2_522 0.58 +0.1 591,658 + Pf6N2E2_522 0.77 +0.2 591,770 + Pf6N2E2_522 0.90 -0.3 591,770 + Pf6N2E2_522 0.90 -1.2
Or see this region's nucleotide sequence